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Citations for Millipore Sigma :
2851 - 2900 of 4915 citations for Recombinant Human CTLA4 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 10ug human epithelial growth factor (hEGF) and 10% FBS (Sigma); LNCaP ...
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bioRxiv - Cell Biology 2023Quote: ... cells were purified on collagen IV from human placenta (Sigma) and human plasma fibronectin (Life Technologies ...
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bioRxiv - Genetics 2023Quote: ... and 5 IU of hCG (Human chorionic gonadotrophin, Sigma-Aldrich) with a 48-hour interval between injections ...
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bioRxiv - Immunology 2023Quote: ... Signals were visualized with anti-human IgG alkaline phosphatase (Sigma, diluted 1:2,000 in PBS 1× + Tween20 0.05% ...
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bioRxiv - Bioengineering 2023Quote: ... we prepared Human Placenta Collagen Type IV (Sigma, catalog #C7521) according to protocols established by University of North Carolina ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 5% human AB serum (Millipore Sigma, Burlington, MA) and 1% MACS supplement (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2023Quote: ... 10 ng mL−1 human leukemia inhibitory factor (hLIF, Millipore), 2 μM SB431542 ...
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bioRxiv - Cell Biology 2023Quote: ... 3% v/v heat-inactivated Human Male AB Serum (Sigma), 2 mg/ml Human Serum Albumin (HSA ...
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bioRxiv - Developmental Biology 2023Quote: ... and 5 IU of hCG (Human chorionic gonadotrophin, Sigma-Aldrich) with a 48-hour interval between injections ...
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bioRxiv - Bioengineering 2024Quote: IgG (from human serum; Sigma-Aldrich, St Louis, MO, USA) and fetal bovine Fetuin (Sigma-Aldrich ...
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bioRxiv - Physiology 2024Quote: ... rabbit-anti-human MUC5B (Sigma, Cat. HPA008246, 1:500 dilution), and biotin-conjugated Ulex europaeus agglutinin-1 (UEA1 ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.023 U/ml human insulin (Sigma-Aldrich, I9278-5ML). HCC1937 cells were cultured in RPMI-1640 medium (VWR ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.01 mg/ml human insulin (Sigma-Aldrich, I9278-5ML). U2OS cells were purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Systems Biology 2024Quote: Ovulation was induced by injection of human chorionic gonadotropin (Sigma) into the dorsal lymph sac according to standard procedure166 and in accordance with approved UCSF IACUC protocols ...
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bioRxiv - Bioengineering 2024Quote: Human fibrinogen from non-diseased donors (Merck Millipore, #341576-1GM) was reconstituted in sterile 0.9% saline to a stock concentration of 67mg/ml ...
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bioRxiv - Bioengineering 2024Quote: Human dermal fibroblasts were available in-house (Sigma, #106-05a). Depending on experiments ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.2 U/ml human insulin (Sigma-Aldrich, I9278-5ML). MCF7 cells were cultured in DMEM (VWR ...
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bioRxiv - Molecular Biology 2024Quote: ... human lymphoblast cells were maintained in RPMI-1640 media (Sigma), and U2OS (ATCC 30-2007 ...
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bioRxiv - Bioengineering 2024Quote: ... was coated with 10ug/ml human fibronectin (EMD Millipore, FC010) for 30 min at 37°C then washed three times with deionized water (DI) ...
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bioRxiv - Immunology 2024Quote: ... and human monoclonal IgE (2 µg/ml; Sigma-Aldrich, #401152) to enhance the expression of the FcεRI before stimulation (Saleh et al. ...
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bioRxiv - Neuroscience 2024Quote: ... and 5 mg/mL human insulin (cat#: I9278; Millipore Sigma), freshly supplemented with 100 ng/mL IL-34 (cat# ...
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bioRxiv - Cell Biology 2024Quote: ... 10 μg/mL human apo-Transferrin (Sigma-Aldrich; cat. #T1147), 2.6 ng/mL sodium selenite (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 mU/mL human insulin (Sigma-Aldrich, I9278-5ML). The fGS/OSS cells were cultured using 25% conditioned medium (from the old culture ...
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bioRxiv - Genetics 2020Quote: ... Approximately 5 mg of extract containing the corresponding recombinant protein was incubated with 25 µl of glutathione sepharose (SIGMA) and subsequently mixed with yeast extracts expressing Sen1 TAP-Nter (typically 0.5 mg of extract per binding reaction) ...
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bioRxiv - Neuroscience 2021Quote: ... parental SK-N-SH cells were trypsinized and resuspended in grafting media in presence of 10μg/ml recombinant NXPH1 (rNXPH1) or 100μg/ml BSA (Ctrl; SIGMA #A7906). To test the growth of NB cells depleted from their α-NRXN1+ subpopulation ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GST-mm-FMN2-eFSI (GST-FMN2-eFSI) proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Cell Biology 2019Quote: A GFP-expressing MDA-MB-231 cell line lacking functional Nav1.5 expression was produced previously by transduction of recombinant lentivirus for shRNA targeting Nav1.5 (MISSION pLKO.1-puro shRNA transduction particles; Sigma) (Nelson ...
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bioRxiv - Pathology 2020Quote: ... each recombinant tau isoform was thawed at room temperature and filtered with a 100 kDa spin column filter (Millipore) to remove unwanted tau aggregates ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were loaded to have roughly similar band intensity for the band of interest to enable better estimation of relative protein size (recombinant pro-conA: 0.75 μg, conA (Sigma): 1.5 μg ...
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bioRxiv - Microbiology 2021Quote: ... neoformans cells were added to washed macrophage monolayers at a 1:1 ratio with 10 units (1 unit = 0.1 ng/mL) of recombinant murine IFNγ (Sigma) and 0.3 μg/ml of lipopolysaccharide (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant E1E2 was extracted from cell membranes using 1% NP-9 and purified via sequential Fractogel EMD TMAE (Millipore), Fractogel EMD SO3− (Millipore) ...
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bioRxiv - Cell Biology 2021Quote: BMDC and BMLC maturation was induced by overnight treatment with 100 ng/ml recombinant murine TNFα (Sigma-Aldrich, USA). In some experiments ...
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bioRxiv - Cell Biology 2021Quote: ... Rose-John), 20 U/ml recombinant murine GM-CSF (PharmedArtis, Germany) and 10−6 M dexamethasone (Sigma-Aldrich, USA). Progenitor cells were amplified 5 to 11 days before differentiation into DC was induced by supplementing 200 U/ml recombinant murine GM-CSF ...
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bioRxiv - Biophysics 2022Quote: ... a heparosan trisaccharide amine derivative was extended with recombinant MBP-PmHS1 synthase using UDP-GlcNAc and UDP-GlcA (Sigma) [48] ...
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bioRxiv - Microbiology 2022Quote: ... coli BL21 (DE3) was used as the host for expressing recombinant proteins encoded in the pET30a vector (Novagen, USA). Appropriate antibiotics were added when needed at the following concentrations ...
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bioRxiv - Biophysics 2020Quote: ... beads were removed and recombinant dynein was concentrated in a 100 KDa cut-off filter (Amicon Ultracel, Merck-Millipore) to a final volume of 500 μL ...
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bioRxiv - Immunology 2020Quote: DHFR activity of the recombinant β-lactamase-DHFR fusion protein was evaluated using the DHFR Assay Kit (Sigma-Aldrich), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... 20 µM DTT and phosSTOP in the presence of recombinant cyclin A2/cyclin-dependent kinase (CDK)1 (Sigma-Aldrich) and 10 mM ATP incubated for 2 h at 30 °C ...
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bioRxiv - Biochemistry 2022Quote: Expression of recombinant extracellular proteins was performed by transient transfection in HEK−293F cells using polyethylenimine (PEI, Sigma Aldrich). 300 mL of culture at a density of 1.0 × 106cells/mL was transfected with 300 μg DNA using 450 μg PEI ...
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bioRxiv - Microbiology 2024Quote: ... 150 mM NaCl) in a 1:3 volume ratio for recombinant enterokinase digestion (Novagen, 1 unit/ 50 μg protein) overnight (Gasparian et al. ...
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bioRxiv - Cell Biology 2023Quote: ... imidazole 5 mM and recombinant RAD51-proteins were purified on a NiNTA resin (#P6611, Sigma, St Louis, MO, USA) using the washing buffer ...
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bioRxiv - Immunology 2023Quote: ... SRBCs in Alsever’s (TCS Bioscience) were conjugated to recombinant HEL3X (R. Brink) with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (Sigma) as previously described104 ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing recombinant PKAc verified via SDS-PAGE were pooled and concentrated to 250μL using an Amicon concentrator (10K MWCO; Millipore). The concentrated sample was then applied to a preparative-grade Superdex200 column (GE Healthcare ...
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bioRxiv - Neuroscience 2023Quote: Primary rat cortical cultures were treated with recombinant Tat 1-86 (Diatheva, Italy) and with cocaine-HCl (Sigma Chemicals) as described in each Figure Legend ...
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bioRxiv - Plant Biology 2023Quote: 6xHistidine-Glutathione-S-transferase (GST) tagged proteins of the predicted intracellular region of SOBIR1 (termed the KD) was cloned into pCDF-Duet vector (Novagen, EMD Chemicals, San Diego, CA) and expressed in E ...
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bioRxiv - Microbiology 2020Quote: ... culture media with serum (DMEM, low glucose, from Sigma-Aldrich; 10% HI-FBS; 1% penicillin-streptomycin solution from Sigma-Aldrich; 1% 200mM L-glutamine from Sigma-Aldrich). All assays were performed with cells in passages 4 to 10.
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bioRxiv - Microbiology 2021Quote: ... from strain BetaCoV/Wuhan/WIV04/2019 was ordered from IDT (Coralville, IA, USA) and cloned into a HIS-SUMO expression vector (a modified pET-DUET; Novagen, Madison, WI, USA). After transformation into BL21(DE3) ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 μM mVP40 proteins or His-tag alone were incubated with 50 μg/ml Ni-NTA-Atto 550 dye (Millipore Sigma, Burlington, MA) in a final volume of 500 μl ...
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bioRxiv - Microbiology 2019Quote: ... The washing step was repeated and 200 µl of a 1:5,000 dilution of the monoclonal Anti-His antibody (Sigma-Aldrich, St. Louis, MO) in PBS + 2% BSA was added to each well ...
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bioRxiv - Cell Biology 2019Quote: ... 20mM reduced glutathione) and analyzed by SDS-PAGE followed by Coomassie staining and immunoblotting with anti-His (dilution: 1:5000; Sigma-Aldrich, Steinheim, Germany), or anti-GFP-antibodies (dilution 1:1000 ...