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Citations for Millipore Sigma :
2851 - 2900 of 10000+ citations for IL 4 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: Astrocytes were plated on PO/Lam coated 96 well plates and treated with 3 ng/mL IL-1α (Sigma; 3901), 30 ng/mL tumor necrosis factor alpha (R&D Systems ...
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bioRxiv - Cell Biology 2019Quote: ... We therefore investigated the bioactive LM profiles in tendon-derived stromal cells isolated from the tendons of healthy volunteers and patients in the presence of IL-1β (10 ngmL-1, Sigma) in medium (DMEM F12 phenol red free medium ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed with PBS-T and 3,3’,5,5’-tetramethylbenzidine peroxidase substrate (TMB, T0440-IL, Sigma, St Louis, MO, USA) was added ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed with PBS-T and 3,3’,5,5’-tetramethylbenzidine peroxidase substrate (TMB, T0440-IL, Sigma, St Louis, MO, USA) was added ...
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bioRxiv - Microbiology 2022Quote: ... according to the manufacturer’s instructions and activated by incubation in the presence of 100 U/ml IL-2 (Sigma Aldrich) and T Cell TransAct™ human (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... expanded in the presence of recombinant human IL-2 (1000 U/mL, Proleukin) and rapamycin (50 nmol/L, Sigma-Aldrich), and transduced after 2 days ...
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bioRxiv - Immunology 2024Quote: ... Human PBMC were pre-activated with 30 ng/ml anti-CD3 (OKT3, Miltenyi) and 50 IU/ml IL-2 (Sigma) and subsequently transduced two times with viral supernatant in the presence of 6 ug/ml polybrene (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 and 7 post IL-12mRNA/scRNA injection (day 11, 13 and 17 post inoculation) and digested in 30% collagenase (Sigma) for 30 minutes at 37°C ...
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bioRxiv - Genetics 2022Quote: ... cells were grown in a basal erythroid medium (BEM) supplemented with hSCF, IL-3, EPO (Eprex, Janssen-Cilag) and hydrocortisone (Sigma). From day 6 to 20 ...
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bioRxiv - Immunology 2023Quote: ... Sorted Treg cultures also contained 1000 U/mL of IL-2 (Proleukin) and 50 nmol/L of rapamycin (Sigma- Aldrich), whereas Tconv cultures contained 100 U/mL of IL-2 ...
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bioRxiv - Neuroscience 2023Quote: ... and reconstituted to 1 mg/mL in sterile water and diluted to 250 μg/mL (IL-9) or 10 μg/mL (IFNγ and IP-10) in 0.1% cell-culture grade bovine serum albumin (Sigma A9418) in 1X PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were cultured in 50µL of IL-11 and SCF media2 for 2h before being supplemented with polybrene (Sigma-Aldrich) and up to 20uL of a lentivirus equipped with the gene of interest (STAT5B-WT or STAT5B-Y699F ...
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bioRxiv - Bioengineering 2024Quote: ... the hydrogel- containing cells were maintained in RoosterBasal™ medium and treated with 1 ng/mL of IL-1β (Sigma) for 4 days at 37 °C and 5% CO2 ...
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bioRxiv - Molecular Biology 2020Quote: ... Mouse anti-Gapdh (clone GAPDH-71.1, cat# G8795) and mouse anti-MBNL1 (clone 3A4) were from Sigma. Rabbit anti-Tubulin (cat# 2148) ...
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REV-ERBα mediates complement expression and circadian regulation of microglial synaptic phagocytosisbioRxiv - Neuroscience 2020Quote: ... 2% Mouse-on-mouse (M.O.M) blocking reagent and 0.4% Triton X-100 (Sigma-Aldrich, St. Louis, MO). Sections were then incubated in TBS containing 10% goat (or donkey ...
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bioRxiv - Cell Biology 2019Quote: ... The CRP concentration in mouse serum was measured using the mouse CRP ELISA kit (Sigma-Aldrich, RAB1121) according to the manufacturer’s protocol.
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bioRxiv - Physiology 2019Quote: Mouse serum insulin levels were determined using the mouse metabolic hormone panel (Millipore Sigma, Milliplex MMHMAG-44K) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... An HRP-conjugated goat anti-mouse IgG or an HRP-conjugated goat anti-mouse IgA (Sigma, USA) was used as the secondary antibody.
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bioRxiv - Immunology 2021Quote: ... An HRP-conjugated goat anti-mouse IgG or an HRP-conjugated goat anti-mouse IgA (Sigma, USA) was used as the secondary antibody.
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bioRxiv - Microbiology 2022Quote: ... with the following modifications: Mouse anti-GST tag or mouse anti-His tag 1:200× (Sigma-Aldrich) and HRP-conjugated goat anti-mouse IgG 1:2,000× (Seracare Life Sciences ...
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bioRxiv - Immunology 2024Quote: ... then incubated with goat anti-mouse secondary HRP antibody (Sigma-Aldrich, #A0168, 1:10,000, for mouse plasma) or goat anti-human secondary HRP antibody (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibody (Mouse Anti-Influenza A NP, Millipore MAB8251 or Mouse Anti-Influenza B NP, Millipore MAB8661) was diluted 1:4000 in antibody diluent ...
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bioRxiv - Microbiology 2023Quote: ... Mouse IZ-actin (Clone AC-74) and mouse FLAG M2 F3165-1MG were obtained from Sigma-Aldrich and used at 1:10 000 and 1:2 000 dilutions in western blot ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse anti-α-tubulin (1:1000; CST3873) and mouse anti-IDH1 (R132H) (1:500, SAB4200548, Sigma-Aldrich).
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bioRxiv - Molecular Biology 2021Quote: Protein-G (Protein G Sepharose® 4 Fast, cat# GE17-0618-01) or Protein-A (ProteinA-Sepharose® 4, cat#P9424 Millipore) beads were washed twice with 1x PBS and twice with IP100 buffer (25 mM Tris-HCl 7.9 ...
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bioRxiv - Neuroscience 2019Quote: ... before fixation with 4% paraformaldehyde/ 4% sucrose (v/v), and subsequently permeabilized (DPBS, 0.2% triton X-100 (Sigma-Aldrich #9002-93-1)) ...
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bioRxiv - Cell Biology 2020Quote: ... and was centrifuged twice at 300 g for 10 min at 4 °C in a Sigma 4-16KS centrifuge (Sigma Laboratory Centrifuges) to remove cells and the supernatant was stored at −80 °C until use.
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with 500nM 4-hydroxytamoxifen (4-OHT) for 72h in 5i+LIF (Supplementary Table1) with 100 μM apoptosis inhibitor Z-vad (Sigma-Aldrich®) and 50 μM of necroptosis inhibitor Necrostatin-1 (Sigma-Aldrich®) ...
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bioRxiv - Neuroscience 2022Quote: ... and the cochleae were fixed in 4% paraformaldehyde (PFA) overnight at 4 °C and then decalcified in 10% EDTA (#798681, Sigma, Darmstadt, Germany) in PBS for 2–3 days at 4 °C ...
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bioRxiv - Genomics 2019Quote: Cells were seeded at a density of 4 × 104 cells per well in laminin coated 4 well chamber slides (Millicell® EZ slide, Merck Millipore, PEZGS0816). Cells were washed in cold PBS and fixed using 4% paraformaldehyde (PFA) ...
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bioRxiv - Cancer Biology 2019Quote: ... Established cell lines were propagated in DMEM containing 10% FBS and maintained with 100nM of (Z)-4-hydroxytamoxifen (4-OHT) (Sigma-Aldrich H7904) and routinely evaluated for mycoplasma contamination ...
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bioRxiv - Neuroscience 2019Quote: ... Specimens for histamine immunohistochemistry were prefixed overnight at 4°C in 4% N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDAC, Sigma–Aldrich E6383). Brains were washed six times for 15 minutes each in PBS and subsequently immersed in Poly-L-Lysin (Sigma-Aldrich ...
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bioRxiv - Genomics 2021Quote: ... three times for 30 minutes each at room temperature and transferred into freshly made X-gal staining solution (4 mM potassium ferricyanide [Sigma-Aldrich, catalog no. P3667], 4 mM potassium ferrocyanide [Sigma-Aldrich, catalog no ...
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bioRxiv - Neuroscience 2021Quote: ... and perfused with 40 ml of 0.1 M/L phosphate-buffered saline and 40 ml of 4% (w/v) PFA (Sigma, 30525-89-4) 24h after social interaction ...
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bioRxiv - Cell Biology 2021Quote: ... diluted to an OD600 of 0.1 the next day and grown up to a mid-log phase (OD600 of 0.8) and labeled with 4-thiouracil (4-tU, Sigma, 2M in DMSO) for 6 min at a final concentration of 5 mM ...
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bioRxiv - Molecular Biology 2022Quote: ... cerevisiae was spiked with S. pombe total RNA (4:1 ratio S. cereviasiae : S. pombe)treated with 4-tU 500 μM final concentration (Sigma-Aldrich, 440736). The biotinylation was performed with 200ul EZ-link-HPDP-biotin (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were washed 3x with 1x PBS and incubated with appropriate primary antibody (Table 4) diluted in 4% BSA (Sigma, A9418-50G) in 1x PBS overnight at 4C ...
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bioRxiv - Microbiology 2024Quote: ... the cells were washed with 500 μl of PBS and fixed with 500 μl of fixative solution (4% formaldehyde, Sigma, and 4% acrylamide, Sigma, in PBS) overnight (O/N ...
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bioRxiv - Cell Biology 2023Quote: ... The organoids thus generated were re-plated at 1:4 split ratio and exposed to 400 nM 4-hydroxy tamoxifen (Sigma; Catalog #H7904) in medium to achieve the deletion of Cbl and Cblb ...
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bioRxiv - Biochemistry 2023Quote: ... 2 µL of sample was mixed with 4 µL of TLC-MALDI buffer (MB, 5 mg/mL α-cyano-4-hydroxycinnamic acid (CHCA, Sigma-Aldrich), 25% acetonitrile ...
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bioRxiv - Genomics 2023Quote: ... untethered Dam and Dam-Cbx1 constructs were fused to an ERT2 domain so that the fusion protein would be translocated to the nucleus upon 4-Hydroxytamoxifen addition (4-OHT, Sigma, Cat#SML1666) (Extended Data Table 1).
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Chemical compounds were first identified using the NIST library and later confirmed with co-elution of synthetic 4-methyl-3-heptanone (Pfaltz and Bauer M19160) and 4-methyl-3-heptanol (Sigma Aldrich M48309). Compounds eluting after 30 minutes were excluded from the analysis due to lack of volatility.
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bioRxiv - Cell Biology 2023Quote: ... Enucleated eyes were immersion fixed in 4% PFA diluted in 1x PBS for 1h at RT and incubated in a hydrogel solution: 4% acrylamide (Sigma, Cat# A4058), 2% bis-acrylamide (Alfa Aesar Cat# J63265) ...
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bioRxiv - Bioengineering 2023Quote: ... cells were fixed with 4% paraformaldehyde and the slide was mounted using a medium containing 4′,6-diamidino-2-phenylindole-DAPI (Fluoroshield, Sigma-Aldrich, USA). A fluorescence microscopy setup (Leica DM 2000 LED fitted with a Leica DFC450c camera ...
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bioRxiv - Cancer Biology 2024Quote: ... for 2 h at 4°C before being rotated overnight at 4°C with Anti-acetyl-Histone H3 (Lys27) (Sigma; 07-360) or Anti-MAFG (GeneTex ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for two days at 4°C with secondary antibody along with 0.01 mg/ml 4′-6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich, Cat#: D9542) to label cell nuclei fluorescently and either 0.2 µl/ml phalloidin-iFluor 594 (Abcam ...
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bioRxiv - Microbiology 2024Quote: ... The samples were fixed overnight at 4°C in the mixture of 4% paraformaldehyde (Cat. No. 162-16065, Wako Pure Chemical Industries) and 2% glutaraldehyde (Sigma-Aldrich, G5882) in PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... Tg(actab2:loxP-BFP-STOP-loxP-dsRed)sd27 fish were treated overnight at 5 dpf with 5 μM (Z)−4-Hydroxytamoxifen (4-OHT) (Sigma-Aldrich H7904) in EM ...
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bioRxiv - Cancer Biology 2021Quote: ... with 4’,6-diamidino-2-phenylindole (DAPI)(Sigma-Aldrich) added to the second wash at to a concentration of 300 nM to counterstain DNA ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then fixed with 4% PFA (SIGMA #16005) for 15min at RT ...