Labshake search
Citations for Millipore Sigma :
2851 - 2900 of 3348 citations for IL 1RA Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... rabbit anti-human βIII-tubulin (1:500; Covance, cat. MRB-435P) and α-tubulin (1:500; Sigma-Aldrich, cat. T9026). Primary antibodies were tagged using either goat anti-mouse AF647 (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... Cytokines secreted by primary CF-HBECs were analyzed by the MILLIPLEX MAP Human Cytokine/Chemokine 48-Plex cytokine assay (Millipore). Cytokines were measure in the basolateral solution only since CF-HBEC were grown in air-liquid interface culture and there was insufficient apical fluid to measure cytokines.
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bioRxiv - Cell Biology 2023Quote: ... The coding sequence of mouse TRAIL (aa118-291) or human TRAIL (aa115-281) was amplified from its cDNA and was cloned into pET21b (Novagen) using NdeI and XhoI sites ...
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bioRxiv - Bioengineering 2024Quote: ... Tumor Necrosis factor alpha (TNF-α)) was carried out with human cytokine/chemokine magnetic bead panel (MilliPlex MAP, Millipore, Sigma). Median fluorescence intensity (MFI ...
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bioRxiv - Bioengineering 2024Quote: ... Tumor Necrosis factor alpha (TNF-α)) was carried out with human cytokine/chemokine magnetic bead panel (MilliPlex MAP, Millipore, Sigma). Median fluorescence intensity (MFI ...
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bioRxiv - Molecular Biology 2024Quote: ... and human epithelial type 2 (HEp-2, CCL-23, ATCC) cells were cultured in Dulbecco’s modified eagle medium (DMEM, Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: CM was collected from TIS MDA-MB-231 cells for chemokine and cytokine analyses using a Milliplex MAP human cytokine/chemokine magnetic bead panel (Merck Millipore) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: ... the HepG2 human hepatocyte carcinoma cell line and the HT29 human colon adenocarcinoma cell lines were purchased from the European Collection of Authenticated Cell Cultures (ECACC) through their distributor (Sigma). The HL1 cell line was purchased from Millipore ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Supplemented IVM media for OSC-IVM consisted of IVM media (Medicult, Origio) supplemented with 75 mIU/ml of recombinant human FSH (Millipore), 100 mIU/ml recombinant human chorionic gonadotropin (hCG ...
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bioRxiv - Molecular Biology 2024Quote: ... Follicles were then cultured in αMEM-based maturation media supplemented with 10% FBS and 1.5 IU/mL Human chorionic gonadotropin (hCG, Sigma-Aldrich) in a an incubator (37°C ...
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bioRxiv - Neuroscience 2024Quote: ... the plate was washed 5 times with PBS and incubated for 1 hour with goat anti-human IgG (Fc specific) –peroxidase (#A0170, Sigma). Binding signals were developed and stopped by the addition of TMB (3,3’,5,5’-tetramethylbenzidine ...
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bioRxiv - Cell Biology 2023Quote: ... Rescue experiments were performed by supplementing the medium with either 25 µg/mL human plasma fibronectin (Millipore Sigma; Catalogue #FC010) or with 10 ng/mL of human TGFβ1 (Peprotech ...
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bioRxiv - Cell Biology 2024Quote: ... Anti-Flag affinity matrix (catalog no. A2220) and albumin from human serum (HSA, Cat No. A3782) was purchased from Sigma. The FITC Goat anti-Rabbit IgG (H+L ...
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bioRxiv - Immunology 2024Quote: ... Antibody for β-actin (Catalog no. A2228) and human α-thrombin (Catalog no. T6884-100N) was purchased from Sigma-Aldrich. All other reagents were bought from Fisher-Scientific except where noted.
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bioRxiv - Microbiology 2024Quote: ... 10 ng recombinant IdeS was pre-incubated with sera dilutions as indicated at 37°C 1 h before addition of 100 ng human IgG (Sigma) in assay diluent (AD ...
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bioRxiv - Cell Biology 2023Quote: ... gondii RH ΔKU80Δhxgprt AtTIR1 and RH TatiΔKU80 strains were grown in Human Foreskin Fibroblasts (HFF) (ATCC, SCRC-1041) in DMEM media (Millipore Sigma) supplemented with 10% FBS [56 ...
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bioRxiv - Immunology 2023Quote: ... EasySep™ Human NK Cell Isolation Kit) and were frozen down in CS10 CryoStor cell cryopreservation media media (Sigma-Aldrich).
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bioRxiv - Microbiology 2023Quote: ... a spectrophotometric stop reaction was used to calculate the activity of human salivary α-amylase (Sigma-Aldrich, cat. No. 1031) using Equation 1 (b = intercept of linear regression ...
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bioRxiv - Synthetic Biology 2023Quote: Stable cell lines expressing human or mouse PRLR were generated by lentiviral transduction followed by 0.7 ug/mL puromycin selection (Sigma; P9620). 12ug of each pTwist Lenti Puro SFFV WPRE lentiviral construct encoding either human or mouse PRLR were co-transfected with 9ug of psPAX2 (Addgene ...
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bioRxiv - Developmental Biology 2023Quote: ... Four- to five-week-old C57Bl/6 females were superovulated and MII oocytes collected in fertilisation media (Human Tubal Fluid (HTF, MR-070, Sigma) containing 4 mg/ml BSA and 25mM L-Glutathione 1 (GSH)) ...
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bioRxiv - Cell Biology 2023Quote: ... Polyacrylamide gels were then immediatly coupled to either human plasma fibronectin (1 mg/mL overnight at 37◦C; FC010, Millipore) or ICAM-1 (0.1 mg/mL Recombinant Protein A (Novex ...
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bioRxiv - Immunology 2023Quote: ... DH270 UCA and anti-c-myc antibody binding were detected with 500 µl of 10 µg/ml goat-anti-human PE (Sigma) and 10 µg/ml goat anti-chicken Alexa 647 respectively ...
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bioRxiv - Biophysics 2024Quote: The tsA-201 cells (a variant of human embryonic kidney HEK cells) were obtained from Sigma-Aldrich (St. Louis, MO). Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Immunology 2023Quote: ... Alkaline phosphatase (AP)-labeled goat anti-human IgM (μ-chain specific; Sigma-Aldrich, Vienna, Austria; 1:50,000 in TBS BSA), AP-labeled goat anti-human IgG (γ-chain specific ...
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bioRxiv - Neuroscience 2023Quote: Human iPSCs were grown to 60-80% confluency and dissociated into a single cell suspension by Accutase (Sigma-Aldrich, A6964) treatment for 5 min at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Exponentially growing RPE-1 (human retinal pigment epithelial) cells were pulse-labeled with CldU (5-Chloro-2’-deoxyuridine, Millipore Sigma) and IdU (5-Iodo-2’-deoxyuridine ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells isolated from healthy donor PBMCs were stimulated by a 1:1 ratio of anti-CD3/28 activation beads (Miltenyi) in complete human T cell medium (RPMI1640 from Sigma containing 2 mM glutamine with the addition of 1 mM pyruvate ...
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bioRxiv - Physiology 2023Quote: ... SVG tissue sections were blocked in a phosphate buffered saline solution containing 0.1% NP-40 and 1% BSA followed by overnight incubation in antibodies targeting human VE-cadherin (Catalog No. MABT886, 1:100 dilution, Sigma) or endothelial nitric oxide synthase (eNOS ...
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bioRxiv - Cell Biology 2023Quote: ... gondii RHΔKu80Δhxgprt AtTIR1 and RHTatiΔKu80 strains were maintained in Human foreskin fibroblasts (HFF) (ATCC, SCRC-1041) cells in DMEM media (Millipore Sigma). Mycoplasma Detection (MP Biomedicals ...
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bioRxiv - Microbiology 2023Quote: ... and HOS-ACE2/TMPRSS2 cells (HOS cells stably expressing human ACE2 and TMPRSS2)31,32 were maintained in DMEM (high glucose) (Sigma-Aldrich, Cat# 6429-500M ...
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bioRxiv - Molecular Biology 2023Quote: Human PBMCs were obtained from the whole blood of AS patients by density gradient centrifugation using Histopaque-1077 (Sigma-Aldrich). Primary human monocytes and CD3+ T cells were isolated from PBMCs using CD14 MicroBeads™ and pan T cell isolation kit (Miltenyi Biotec) ...
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bioRxiv - Genetics 2023Quote: ... Then cells were resuspended in the BD-FACS staining buffer and labelled through anti-human TRA-1-60 antibody (Millipore) and anti-mouse IgM control ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were grown in human plasma-like medium according to the published formulation (15) with 5% dialyzed fetal bovine serum (FBS) (Sigma) and pen/strep (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... MIA PaCa-2 cells were treated with 0.1 % v/v DMSO vehicle control or with compounds at 37 °C for 4 h and then stimulated with 200 ng/mL human epidermal growth factor (hEGF, #E9644, Sigma) at 37 °C for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... The following antibod-ies were used for western blots of human cell extracts: Mouse anti-FLAG (Sigma F1804, 1:1000); Mouse anti-GAPDH (Proteintech 60004-1-Ig ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... A constant amount of plasmid cDNA (15 μg) was transfected into human embryonic kidney cells 293 T (HEK-293T) using polyethylenimine (PEI; Sigma) in a 1:2 weight ratio in 10 cm plates ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with the primary antibody OC (1:500 for mouse, 1:200 for human; polyclonal, AB2286, Millipore) and respective secondary antibody (1:400 ...
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bioRxiv - Molecular Biology 2023Quote: ... The process was the same as in fibroblasts and the primary antibodies were anti-human NANOG (1:100, Millipore #AB5731) and SSEA4 (1:25 ...
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bioRxiv - Pathology 2023Quote: ... human RPE cells were subjected to treatment with 0.2 μg/ml doxorubicin (DOX) (Sigma-Aldrich, #D1515, St. Louis, MO, USA) for a duration of 3 hours in a culture medium containing 0.5% fetal bovine serum (FBS) ...
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bioRxiv - Microbiology 2023Quote: ... parasites were cultured within O+ human erythrocytes at 5% haematocrit in RPMI 1640 complete medium containing L-glutamine and 25 mM HEPES (Sigma), supplemented with 0.2% sodium bicarbonate ...
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bioRxiv - Cell Biology 2023Quote: The C19orf43.WT plasmid is comprised of an N-terminal 3x Flag tag followed by human full length C19orf43 cloned into the p3XFlag-CMV-10 vector (Sigma). C19orf43.R7G was obtained by mutating the arginine at position 7 to glycine and C19orf43.R106G was obtained by mutating the arginine at position 106 to glycine ...
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bioRxiv - Physiology 2023Quote: Glycerol stocks of pLKO.1-puro lentiviral plasmid vectors containing shRNA targeting human Nup93 (NM_014669) and an empty vector insert (shEmpty) were purchased from Sigma-Aldrich. Lentivirus expressing shNup93 or shEmpty were generated by PEI-mediated co-transfection of pLKO.1-puro ...
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bioRxiv - Bioengineering 2023Quote: ... encoding the human KRASG12V and KRASY64A mutants (residues 1–169) and human SOS1 (residues 564–1049) were cloned as NdeI and XhoI fragments into pET28b (Novagen). His-tagged KRAS•GDP (abbreviated RAS ...
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bioRxiv - Immunology 2022Quote: ... in mid-logarithmic phase was added at a multiplicity of infection of 2 in RPMI with 10% human serum (Sigma). Bacteria were spun down for 5 min at 800 x g and incubated for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... and Mirg-/-littermates injected subcutaneously with Growth factor-reduced Matrigel BD) with 400 ng mL-1 recombinant human bFGF (Millipore). After 7 days Matrigel plugs ...
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bioRxiv - Cancer Biology 2023Quote: The mouse E0771 (derived from a spontaneous triple negative mammary tumor that arose in a C57BL/6 mouse) and the human K562 (originally generated from a female leukemia patient) cell lines were cultured in RPMI-1640 (Sigma) supplemented with 10% FCS (GIBCO) ...
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bioRxiv - Developmental Biology 2023Quote: ... (gift from the laboratory of Jeremy Nathans) rabbit anti-LW/MW-opsins (1:200 for organoids, 1:500 for human retinas) (AB5405 Millipore), and mouse anti-Rhodopsin (1:500 ...
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bioRxiv - Immunology 2023Quote: ... in FACS-blocking buffer (mixture of 0.66% human/rabbit/mouse serum, Sigma-Aldrich, and 1% Bovine Serum Albumin, Sigma-Aldrich) for 40 minutes at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... followed by streptavidin-Cyanine-3 (1:500; Amersham Bioscience) or by goat anti-human IgG coupled to FITC (1:100, Sigma), as appropriate ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... Immunofluorescence in human cells was conducted as previously described using antibodies against tubulin (1:1000 anti-beta tubulin, mouse, T7816, Sigma OR 1:2000/1:1000 anti-alpha tubulin ...