Labshake search
Citations for Millipore Sigma :
2851 - 2900 of 10000+ citations for Catenin alpha 1 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... 20 ng/ml recombinant human epidermal growth factor (hEGF, #E9644, Sigma), 0.25 ng/mL hydrocortisone (#H0888 ...
-
bioRxiv - Bioengineering 2022Quote: ... and recombinant insulin human (≥ 98%) were all purchased from Sigma-Aldrich. Water (LC-MS grade) ...
-
bioRxiv - Genomics 2022Quote: ... 1000 U/ml recombinant leukemia inhibitory factor (LIF/ESGRO) (Merck Millipore), and 50U/ml Penicillin/Streptomycin (Thermo Fisher Scientific) ...
-
bioRxiv - Biochemistry 2024Quote: ... 10 µg/ml of recombinant human insulin (Sigma-Aldrich, 19278-5ML), 2 IU/ml heparin (Sigma-Aldrich ...
-
bioRxiv - Immunology 2024Quote: ... and 5 ng/ml of mouse recombinant IL-4 (Sigma-Aldrich) (L-I) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Recombinant human Hepatocyte Growth Factor (HGF) was obtained from Sigma Aldrich Israel ...
-
bioRxiv - Biophysics 2022Quote: ... The recombinant proteins were first purified by Ni2+-affinity column (Novagen) under native conditions and subsequently in-gel cleavage by TEV protease was conducted ...
-
bioRxiv - Developmental Biology 2023Quote: ... 103 units/mL ESGRO recombinant mouse LIF protein (Millipore Sigma, ESG1107), 2 μM PD0325901 (Tocris ...
-
bioRxiv - Plant Biology 2023Quote: ... The recombinant fusion protein was purified with Glutathione Sepharose beads (Sigma). Definite probes were designed for different cis-elements and their mutated versions (Table S3) ...
-
bioRxiv - Systems Biology 2024Quote: ... and 500 U/mL recombinant leukemia inhibitory factor (LIF; Millipore, ESG1107) at 37 °C in 5% CO2 ...
-
bioRxiv - Developmental Biology 2024Quote: ... Recombinant protein was produced in Escherichia coli strain BL21(DE3) (Novagen). The cells were grown in LB medium at 37°C and the protein expression was induced at OD600 of 0.7 with 0.2 mM IPTG (Isopropyl β-d-1-thiogalactopyranoside ...
-
bioRxiv - Cell Biology 2023Quote: Human recombinant Cdk9/cyclin K and CDK9/cyclin T (Sigma Aldrich) were incubated with the substrate at 30°C for 30 minutes in reaction buffer (25 mM Tris-acetate (pH 7.9) ...
-
bioRxiv - Bioengineering 2024Quote: ... 500 μg/mL recombinant human albumin (Sigma-Aldrich, cat. no. A9731), and 213 μg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich ...
-
bioRxiv - Evolutionary Biology 2024Quote: All recombinant proteins were expressed using pET-11a (Sigma #69436-3) based vectors in BL21(DE3 ...
-
bioRxiv - Cell Biology 2024Quote: ... recombinant full-length human active AAK (#14-511 Merck Millipore, Germany) were incubated either in the presence or the absence of 14nM of N-terminal GST-fused active HsMPS1/TTK (SignalChem ...
-
bioRxiv - Developmental Biology 2020Quote: ... individual components can be added to 500 ml media bottle at the indicated final concentrations: 1) Recombinant Human Insulin (Sigma I-1882) – 12.5 µg/ml final concentration ...
-
bioRxiv - Evolutionary Biology 2022Quote: The enzyme assay for the zebrafish Irg1-like was performed by incubating 7 µg recombinant protein with 1 mM cis-aconitate (Sigma A3412-1G) in 300 µl reaction buffer (10 mM HEPES ...
-
bioRxiv - Developmental Biology 2022Quote: ... containing the same supplements) freshly supplemented with 25 ng/mL of human recombinant FGF4 (prepared in house) and 1 μg/mL heparin (Sigma-Aldrich, USA). TSCs were passaged every 3-5 days ...
-
bioRxiv - Immunology 2024Quote: ... MASP-3 and MAP-1 was measured in an ELISA assay where 10 µg/ml recombinant human albumin (rHA) (Sigma-Aldrich, A9731) was coated on Nunc MaxiSorp flat-bottom 96-well plates (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2023Quote: BCBL-1/Cas9 cells containing pLenti SpBsmBI Guide Hygro (AAVS1 or TRAIL-R1 #1/2) were treated with the indicated serial dilutions of recombinant human TRAIL (Sigma-Aldrich, T9701) and absolute cell numbers were measured 24 hours later by flow cytometry as described above for cumulative growth curves.
-
bioRxiv - Neuroscience 2023Quote: ... CaBP (1:2000, rabbit, CB38, Swant or 1:2000, mouse, #300) and VGLUT2 (1:7000, guinea pig, AB2251, Millipore). The slices were washed 3 times in 1% Triton X-100 in PBS for 5 to 10 minutes ...
-
bioRxiv - Microbiology 2019Quote: ... Cells were incubated with purified mAbs (0.1-2.0 µg/mL) diluted in 10% NGS (Sigma-Aldrich) / 0.1% saponin ...
-
bioRxiv - Molecular Biology 2022Quote: ... The primary antibodies purchased from commercial sources are listed below: mouse anti-HA mAb (Sigma, USA), mouse anti-FLAG mAb (Sigma ...
-
bioRxiv - Biochemistry 2021Quote: ... was initially subjected to affinity chromatography using a protein-A/anti-BSA mAB matrix (Sigma-Aldrich) to remove excess albumin (a heme-binding protein contaminant) ...
-
bioRxiv - Immunology 2021Quote: ... were then added to the mAb solution supplemented with 0.25% guinea pig complement sera (Sigma, Israel) and the mixture incubated at 37°C ...
-
bioRxiv - Biophysics 2020Quote: ... pH 7.4 for affinity purification of the FLAG-tagged cHMM on M2 mAb-Sepharose beads (Sigma). Bound cHMM was eluted with 0.1 mg⋅mL−1 FLAG peptide (Sigma) ...
-
bioRxiv - Immunology 2020Quote: ... Antibody binding was detected with goat anti-mouse IgG mAb conjugated with AlexaFluor 647 fluorochrome (Sigma). Sample acquisition was performed on a LSR Fortessa-X20 cytofluorimeter (BD biosciences ...
-
bioRxiv - Cell Biology 2022Quote: ... Mouse anti-b-actin mAb (clone AC-15; #A1978) was obtained from Sigma-Aldrich (MO, USA). Rabbit anti-GFP pAb was from MBL (Nagoya ...
-
bioRxiv - Microbiology 2023Quote: ... HCoV-OC43 rN was additionally characterized by using mouse anti-OC43 N mAb (Sigma-Aldrich # MAB9013) followed by IRDye® 800CW Goat anti-Mouse IgG Secondary Ab (LI-COR # 926-32210).
-
bioRxiv - Biochemistry 2023Quote: ... Purified mAbs in PBS were then filtered using sterile 0.2-μm pore size filter devices (Millipore), and stored in aliquots at 4°C until future use.
-
bioRxiv - Microbiology 2023Quote: ... incubated with mouse anti-HA mAb clone 12CA5 conjugated with protein G agarose beads (Sigma-Aldrich) for 16 h at 4°C ...
-
bioRxiv - Cell Biology 2019Quote: ... and incubated overnight at 4°C with primary antibodies (rabbit anti-TMEM98, Proteintech, 1:1000; rabbit anti-FLAG polyclonal, Sigma, 1:2000 ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were immunodetected using 1:2500 dilution of the rabbit anti-TbKH polyclonal antibody and 1:15,000 dilution of goat anti-rabbit-HRP antibody (Sigma-Aldrich). The chemiluminescent protocol was used for developing as indicated below.
-
bioRxiv - Genetics 2019Quote: ... The infectious blood meal was composed of a virus suspension diluted (1:3) in washed rabbit blood and resuspended at 50% (vol/vol) in dialyzed rabbit serum (R4505; Sigma). ATP was added to a final concentration of 5 μM ...
-
bioRxiv - Plant Biology 2024Quote: ... and subjected to immunoblot analysis with the relevant primary and secondary antibodies (anti HA rabbit polyclonal, Abcam ab20084; anti AV2 CP rabbit polyclonal, DSMZ RT-0953-0645/1; anti c-Myc mouse monoclonal, Sigma M4439 ...
-
bioRxiv - Cell Biology 2023Quote: ... The pre-cleared chromatin was then incubated overnight at 4°C with 10 µL of either rabbit IgG (1 mg/mL, IGBMC facility) or rabbit anti-CTCF (07-729, Millipore). Immunoprecipitated chromatin was then adsorbed to 100 µL prepared protein A/protein G-Dynabeads for 3.5 h at 4°C on a rotating wheel ...
-
bioRxiv - Molecular Biology 2021Quote: ... rabbit anti-V5 (PRB-189P, Covance, 1/1000) or mouse anti-FLAG (M2, Sigma, 1/1000) antibodies ...
-
bioRxiv - Microbiology 2020Quote: Antibodies against LeishIF4E2(rabbit polyclonal, 1:2,000) and against the SBP tag (Millipore, monoclonal, 1:10,000), were used to detect the endogenous and tagged LeishIF4E2 proteins ...
-
bioRxiv - Microbiology 2021Quote: ... The following antibodies were used for specific protein detection: anti-PAP (1: 1:5000, rabbit, Sigma), anti-myc (mouse ...
-
bioRxiv - Neuroscience 2022Quote: ... 29] for astrocytes (rabbit anti-GFAP 1:500, Dako, mouse anti-α-tubulin 1:500, Sigma) and microglia (rabbit anti-Iba1 ...
-
bioRxiv - Cell Biology 2022Quote: ... Primary antibodies: goat anti-CB1R primary (1:2,500) and rabbit anti-actin (1:2,500; Sigma Aldrich). Secondary antibodies ...
-
bioRxiv - Cell Biology 2021Quote: ... anti-Rac1 (1:250, Cytoskeleton, Cat. # ACC03, Polyclonal rabbit anti-GAPDH (1:10,000: AB2302; EMD Millipore), Monoclonal mouse anti-phospho-myosin-light-chain (1:500 ...
-
bioRxiv - Neuroscience 2020Quote: The following antibodies were used rabbit and chicken anti-GFP (1:2000, Millipore; 1:1000, Abcam); goat anti-choline acetyltransferase (ChAT ...
-
bioRxiv - Neuroscience 2022Quote: ... membranes were incubated with secondary antibodies for 1 hour (mouse and rabbit HRP, 1:1000, Millipore). Immunoblot signals were detected using an ECL detection kit (PerkinElmer Life Sciences ...
-
bioRxiv - Biochemistry 2023Quote: ... followed by incubation with anti-Sulf-1/2 primary antibody (anti-Sulf-1: rabbit polyclonal, Sigma, SAB1410410 ...
-
bioRxiv - Microbiology 2024Quote: ... 1% BSA at 4 °C overnight: rabbit anti-microtubule-associated protein 2 (MAP2, Millipore 1: 200); sheep polyclonal anti-dopamine beta hydroxylase (DBH ...
-
bioRxiv - Genetics 2020Quote: ... Cells were spun down and resuspended in fresh SC-Dex media containing 24 nM alpha factor (Millipore-Sigma). Cells were incubated at 30°C for 2 hours to induce arrest in G1 phase.
-
bioRxiv - Genetics 2020Quote: ... Cells were spun down and resuspended in fresh SC-Dex media containing 24 nM alpha factor (Millipore-Sigma). Cells were incubated at 30°C for 2 hours to induce arrest in G1 phase.
-
bioRxiv - Immunology 2020Quote: ... and the protein was eluted with MES wash buffer supplemented with 500 mM methyl-alpha-D-mannopyranoside (Sigma). The protein was buffer exchanged into phosphate buffered saline by successive rounds of centrifugation and stored at −80°C.
-
bioRxiv - Genetics 2020Quote: ... log phase cells (OD600~0.5 - 0.6) with bar1Δ were arrested in late G1 phase using 50 ng/ml alpha-factor peptide (Sigma) for ~120 minutes ...