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Citations for Millipore Sigma :
2801 - 2850 of 7712 citations for West Nile Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Solubilized protein was loaded onto 1.5 mL of M2 affinity matrix (Sigma) equilibrated with wash buffer (20 mM MES pH 6.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The concentrated protein was immobilized on cyanogen bromide activated Sepharose 4G (Sigma) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... the reduced proteins were alkylated with 150 mM Iodoacetamide (Millipore, Bedford, USA) in darkness at room temperature for 30 min ...
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bioRxiv - Biochemistry 2022Quote: All proteins were reduced by incubating with 10 mM of dithiothreitol (Sigma) at 56 °C and alkylated by 27.5 mM of iodoacetamide (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... DNA-protein complexes were crosslinked using 1% final concentration of formaldehyde (Sigma) for 10min at RT ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were then transferred to 0.2 μm nitrocellulose membranes (Millipore, 10600001) using a wet tank system at 115 V for 1 h at 4°C in Tris-Glycine buffer (25 mM Tris ...
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bioRxiv - Immunology 2022Quote: ... Commercially available flavivirus E protein-specific mAb 4G2 (EMD Millipore, Darmstadt, Germany), rabbit anti-ZIKV prM and C antibodies (GeneTex ...
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bioRxiv - Genetics 2022Quote: ... and 0.1% sodium dodecyl sulfate) containing a protein inhibitor cocktail (Sigma-Aldrich). The homogenate was centrifuged for 10 minutes at 10,000g and the supernatant was collected ...
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bioRxiv - Plant Biology 2022Quote: ... Protein concentrations were determined using a Direct Detect Infrared Spectrometer (Millipore Sigma).
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bioRxiv - Molecular Biology 2022Quote: ... we used the protein synthesis inhibitor cycloheximide (CHX, Sigma-Aldrich, Deisenhofen, Germany). CHX inhibits eEF2-mediated translocation in eukaryotic ribosomes (Schneider-Poetsch et al. ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-microtubule-associated protein 2a+b (MAP2, Sigma #M1406; 1:2000), rabbit anti-tyrosine hydroxylase (TH ...
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bioRxiv - Plant Biology 2022Quote: ... and fusion proteins purified according to manufacturer’s instructions (GE Healthcare or Novagen). Protein-protein binding buffer contained ...
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bioRxiv - Neuroscience 2022Quote: ... RRID:AB_839504) and myelin basic protein (MBP; rat anti-MBP; #MAB386, Millipore; RRID:AB_94975).
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bioRxiv - Neuroscience 2022Quote: Total protein concentrations were measured using the Pierce BCA method (Sigma, USA). Equivalent amounts of protein (40 mg per lane ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated using SDS-PAGE and transferred to PVDF membrane (Millipore). Immunoblotting was achieved using the indicated antibodies listed in the supplemental table 3 ...
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bioRxiv - Neuroscience 2023Quote: ... Protein bands were revealed with enhanced chemiluminescence reagent (211595, Millipore, Immobilon Crescendo) and images captured with a KwikQuant Imager (Kindle Biosciences) ...
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bioRxiv - Neuroscience 2023Quote: ... Anti-Myelin Basic Protein antibody [12] (MBP) (Millipore; rat monoclonal, 1:500), Mouse anti-A2B5 (Invitrogen ...
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bioRxiv - Physiology 2023Quote: ... Oxygen consumption rates were calculated and normalized for protein content by Novagen BSA assay (71285-3 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Eluted proteins were desalted using either Amicon Ultra centrifugation filters (Merc Millipore) or Zeba Spin desalting columns (Thermo Fisher scientific ...
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bioRxiv - Immunology 2022Quote: ... samples were incubated in the presence of Protein A Sepharose beads (Millipore) for 1-2h ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were visualized using chemiluminescence (Immobilon Western Chemiluminescent HRP Substrate, Millipore, #WBKLS0500) on Bio-Rad ChemiDoc (XRS+) ...
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bioRxiv - Neuroscience 2022Quote: ... and the protein concentration determined with a BCA assay (Sigma, Deisenhofen, Germany). Equal amounts of protein samples were separated on 8% Tris/glycine gels and then transferred to nitrocellulose membranes (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... Transferring the protein from the gel to the PVDF membrane (Millipore #ISEQ00010). Membranes were blocked with 5% milk for one hour at room temperature and incubated with appropriate dilutions of primary antibody overnight at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... in the presence or absence of 0.5 mg/mL OVA protein (Sigma) in cRPMI at 37℃ in 5% CO2 incubator for 72 hours ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were detected with anti-Flag antibody (Sigma, F1804, monoclonal, 1:1,000) or anti-Sso antibody (rabbit antiserum ...
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bioRxiv - Cell Biology 2022Quote: ... The protein complex was concentrated using centrifuge filters (Amicon Ultracel-30K, Millipore) and subjected to SEC using a Superdex 200 column (HiLoad 16/600 Superdex 200 pg ...
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bioRxiv - Physiology 2022Quote: ... Protein extraction was carried out on ice using RIPA buffer (Sigma Aldrich) supplemented with protease inhibitor cocktail (Sigma) ...
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bioRxiv - Bioengineering 2022Quote: ... and then filtered through a 0.45-μm low protein binding membrane (Millipore). HEK293T cells were seeded into 12-well plates at approximately 40-50% confluency and 50 μl filtered virus-containing supernatant was added to the 12-well plates ...
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bioRxiv - Neuroscience 2022Quote: ... the expression of GST fusion proteins was induced with isopropylthiogalactoside (IPTG, Sigma) at 16 °C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were detected using an enhanced chemiluminescent detection system (EMD Millipore, #WBKLS0500) and ChemiDoc Imaging System (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... Protein bands were visualized with Immobolin Forte Western HRP substrate (Sigma, WBLUF0100) and imaged with a ChemiDoc Imaging System (BioRad) ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were visualized using Immobilon Western Chemiluminescent HRP Substrate (Millipore Sigma, WBKLS0500) on Pro Signal Blotting film (Genesee Scientific ...
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bioRxiv - Immunology 2023Quote: ... proteins (50 μM) and TCEP (5 mM) reducing agent (Sigma Aldrich, USA) were mixed 1 hour at RT and then left overnight (O/N ...
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bioRxiv - Pathology 2024Quote: ... and the blotted protein bands were revealed by the ECL system (Millipore).
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bioRxiv - Microbiology 2024Quote: ... the separated proteins were transferred onto Immobilon-P PVDF membrane (Merck Millipore). The membrane was blocked with 10% skim milk in Tris Buffered Saline ...
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bioRxiv - Microbiology 2024Quote: ... The cleaved protein was concentrated using Amicon 10 KDa MWCO concentrators (Millipore), and further purified by size exclusion chromatography using a Superdex 200 (16/60 ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were then transferred onto Immobilon-P PVDF membranes (#IPVH00010; Millipore) for immunoblotting ...
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bioRxiv - Cancer Biology 2024Quote: mTOR kinase domain protein was purchased from Sigma-Aldrich (Cat # 14-770). ETV7 protein (Cat # TP307742 ...
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bioRxiv - Physiology 2024Quote: ... Proteins were then transferred to PVDF membranes (Millipore, Burlington, MA, USA, #IPFL00010) in NuPAGE transfer buffer at 20V for 60 minutes at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... MAPK6 and MKP2 protein were detected with anti-AtMPK3 antibody (Sigma M8318), anti-AtMPK6 antibody (Sigma M7104 ...
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bioRxiv - Microbiology 2024Quote: ... Optimal protein expression and solubility was achieved in RosettaTM(DE3) pLysS (Novagen) for wild-type His::PfPKG ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were then transferred to 3 kDa protein-filter columns (Sigma-Aldrich) and centrifuged at 12,000g for 45 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... Rabbit polyclonal antibody against protein disulfide isomerase (1:100) (PDI; P7122, Sigma). Secondary antibody Alexa Fluor 555 (A-21422 ...
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bioRxiv - Neuroscience 2024Quote: ... Mouse Anti-Glial Fibrillary Acidic Protein (1:100, Millipore, Cat# MAB360, RRID:AB_11212597), Mouse Anti-NeuN (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-amyloid precursor protein (APP, C-terminal, 1:1000, Sigma, #A8717), rat anti-green fluorescent protein (GFP ...
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bioRxiv - Immunology 2024Quote: ... Protein extracts were immunoprecipitated with a PP2A C-specific antibody (1D6; Millipore). An appropriate phosphorylated peptide (amino acid sequence K-R-pT-I-R-R ...
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bioRxiv - Microbiology 2024Quote: ... The procedure involved reducing the proteins with 10 mM DTT (Sigma-Aldrich), followed by carbamidomethylation with 100 mM iodocetamide (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... Protein bands were detected by Luminol-based enhanced chemiluminescence (EMD Millipore WBKLS0500). Immunoblots were captured on film and quantified using Image J.
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bioRxiv - Biophysics 2024Quote: ... BCA Protein Assay Kit and oligonucleotides were purchased from Sigma Aldrich (international). Low melting temperature agarose was purchased from Fisher Bioreagents (Massachusetts ...
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bioRxiv - Cell Biology 2023Quote: ... we precipitated the eluted proteins by adding trichloroacetic acid (#T0699, Sigma Aldrich) to 20% final concentration ...