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Citations for Millipore Sigma :
2801 - 2850 of 10000+ citations for Mouse FAM117A shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: Plasmids for sgRNA expression were cloned by annealing synthetic oligonucleotides (Sigma-Aldrich) and ligating them into the appropriate vector ...
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bioRxiv - Microbiology 2022Quote: The C1-07020 gene was cloned into the plasmid pETDuet-1 (Novagen) and then transformed into E ...
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bioRxiv - Bioengineering 2022Quote: ... DogCatcher-NANP18 and SpyCatcher were cloned into expression plasmid pET45(+) (EMD Millipore) for protein production in BL21(DE3 ...
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bioRxiv - Biochemistry 2021Quote: ... Retromer plasmids were co-transformed into BL21(DE3) Rosetta2 pLysS cells (Millipore). Cells were grown to OD600 between 0.8-1.0 and induced for 16-20 hours at 20°C with 0.4 mM IPTG ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmid pET28a(+) was purchased from Novagen (EMD Biosciences, San Diego, CA, USA). Preparation of plasmid pACYCcamAB for coexpression of putidaredoxin reductase (CamA or PdR ...
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bioRxiv - Molecular Biology 2022Quote: ... All plasmids were prepared in Stbl3 or DH5α using MiniPrep (Sigma-Aldrich) or MidiPrep (Macherey-Nagel ...
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bioRxiv - Biochemistry 2019Quote: ... the pRSFMBP vector was constructed based on the plasmid pRSFDuetTM-1 (NOVAGEN) with the replacement of the coding sequence for the His-tag by that of MBP (Maltose Binding Protein ...
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bioRxiv - Neuroscience 2019Quote: ... DNA plasmids mixed in saline (PBS) solution and 0.025% Fast Green (Sigma) were injected through the uterine wall into the lateral ventricle of each embryo ...
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bioRxiv - Developmental Biology 2020Quote: ... Plasmids were transfected in respective iFNP cells cultured in DMEM (Sigma #5796) supplemented with 10% calf serum with 50 U/mL each penicillin and streptomycin ...
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bioRxiv - Synthetic Biology 2019Quote: ... The digested plasmids were checked by agarose gel electrophoresis (1% agarose (Sigma), 1xTris-boric acid EDTA buffer ...
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bioRxiv - Microbiology 2020Quote: ... The resulting plasmid (pBEL1307) was transformed into Rosetta 2 (DE3) cells (Novagen). For expression ...
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bioRxiv - Pathology 2021Quote: ... The lentiviral plasmids (LV01) and lentivirus production were obtained from Sigma-Aldrich. Cells were sorted for GFP expression by flow cytometry and maintained in puromycin (3μg/mL) ...
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bioRxiv - Biochemistry 2021Quote: ... The digested PCR product was cloned into a pET-52b(+) plasmid (Novagen) in frame with an N-terminal Strep-tag II obtaining the B35SSB expression vector pET52b::B35SSB ...
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bioRxiv - Genetics 2021Quote: ... coli using the GenElute Plasmid Miniprep Kit (Sigma-Aldrich, St. Louis, MO) or the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: HEK293T cells transfected with indicated plasmids were lysed using SDC (D6750, Sigma) buffer (4% SDS ...
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bioRxiv - Cell Biology 2021Quote: ... plasmids were transformed into BL21(DE3)pLysE bacteria (Sigma-Aldrich, CMC0015-20X40UL) and grown in TB supplemented with 50 µg/mL kanamycin and 33 µg/mL chloramphenicol at 37 °C until an OD600 of 0.4 was reached ...
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bioRxiv - Developmental Biology 2022Quote: ... Plasmid DNA was mixed with Fast Green FCF (final 0.05%; F7252, Sigma) and injected at 2μg/μl into the cochlear duct using the Wiretroll plunger system (53507-426 ...
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bioRxiv - Genetics 2022Quote: ... Transgenic cells were selected with puromycin (sgRNA plasmids) (1 ng/μl, Sigma) or hygromycin (FIREWach plasmids ...
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bioRxiv - Microbiology 2022Quote: ... NS5 coding sequences were subsequently transferred into expression plasmid pET-15b (Novagen) using standard molecular biology procedures (Supplementary Figure S1) ...
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bioRxiv - Cancer Biology 2024Quote: calf thymus DNA and supercoiled plasmid pUC19 DNA were obtained from Sigma Chemical Company ...
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bioRxiv - Synthetic Biology 2023Quote: ... lac operon amplicon was amplified from the pETDuet-1 plasmid (Novagen 71146) and cloned into the pBBR1-MCS1 plasmid to construct the pBBR1-MCS1-plac plasmid ...
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bioRxiv - Neuroscience 2023Quote: ... and 32.5µg gag-pol packaging plasmid (pCMVΔR8.74) using 10 mM polyethylenimine (Sigma) in OptiMEM for three hours (48) ...
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bioRxiv - Plant Biology 2023Quote: ... ATG3 coding regions were cloned into the pET28a (+) plasmid (Novagen, 69864-3) for expression of the corresponding His-tagged protein ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids were transformed into Escherichia coli BL21 Tuner (DE3) strain (Sigma-Aldrich) or Rossetta DE3 (Novagen ...
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bioRxiv - Microbiology 2023Quote: ... or control plasmid expressing 3xFLAG-tagged TRIM25 through X-tremeGENE9 (Millipore Sigma). Twenty-four hours later ...
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bioRxiv - Immunology 2023Quote: ... 293T cells were transfected with premixed plasmids and PEI (MW25000Da, Sigma, USA) and incubated for 72 h ...
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bioRxiv - Cell Biology 2023Quote: ... transfection agent with 3rd generation packaging plasmids and either shScr (SHC332; Sigma), shYAP (addgene #42541) ...
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bioRxiv - Microbiology 2023Quote: ... This plasmid carries the pRSF1030 replicon from RSFDuet™ (Novagen, Merck Millipore), araC and the araBAD promoter from pBAD/His (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... This plasmid carries the pRSF1030 replicon from RSFDuet™ (Novagen, Merck Millipore), araC and the araBAD promoter from pBAD/His (Invitrogen) ...
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bioRxiv - Cancer Biology 2024Quote: 293T cells were transfected with the modified plasmid using GeneJuice (Novagen 70967) mixed with a combination of the assembled plasmid containing the luciferase tag ...
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bioRxiv - Cancer Biology 2024Quote: ... lentivirus containing plasmids from either non-target short hairpin RNA (Sigma #SHC0020) or shCSPG4 (gene target NM_001897 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... DNA was isolated and purified by GeneEluteTM Plasmid Miniprep kits (Sigma, USA). Presence of the mutations was verified by DNA sequencing (The Applied Genomics Core ...
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bioRxiv - Synthetic Biology 2024Quote: ... These cell suspensions were midiprepped (GenElute HP Plasmid Midiprep Kit, Millipore Sigma), and the final pools of library DNA were used for lentivirus production and titering at the University of San Francisco Viracore ...
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bioRxiv - Cancer Biology 2020Quote: ... then incubated with primary antibodies (mouse anti-PAR, 1:400 Merck Millipore Cat#AM80 RRID: AB_2155072; mouse anti-γH2AX pS139, 1:2000 Merck Millipore Cat#05-636 RRID ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were immunoblotted with rabbit monoclonal anti-mouse caveolin-1 and rat monoclonal anti-mouse total β1-integrin (clone MB1.2, MAB1997 Millipore) as a loading control and a marker for PM fraction ...
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bioRxiv - Cell Biology 2022Quote: ... used for HA::SYP-1) or mouse anti-HA (SYP-4) and mouse anti-α-tubulin (DM1A, EMD Millipore), each diluted 1:5,000 ...
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bioRxiv - Physiology 2023Quote: ... Mouse monoclonal anti-FLAG M2 antibody (F1804) and mouse monoclonal anti-β-actin antibody (A1978) were obtained from Sigma. HRP-conjugated goat anti-rabbit (CST#7074 ...
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bioRxiv - Immunology 2023Quote: ... the bound antibody was incubated with horseradish-peroxidase labelled subclass-specific anti-mouse IgG1 at 1:8,000 or anti-mouse IgG2c at 1:2,000 (Sigma, Aldrich). After five washes with PBS-T ...
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bioRxiv - Immunology 2022Quote: ... we used the appropriate corresponding isotype control antibodies (mouse IgG1 K and mouse IgG2 B, both from Biolegend, goat IgG from SIGMA) and a drug carrier control.
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bioRxiv - Immunology 2021Quote: The levels of 32 different cytokines and chemokines in mouse sera were measured using a Milliplex MAP Mouse Cytokine/Chemokine premixed 32 Plex (Millipore), following the manufacturer’s protocols ...
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bioRxiv - Bioengineering 2020Quote: ... the plates were incubated with the mouse anti-HA antibody followed by goat anti-mouse IgG-alkaline phosphatase (Sigma-Aldrich). The absorbance at 405 nm was read by the microplate reader (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... served as blocking buffer and monoclonal mouse-derived anti-HIS antibody (LS-C57341, LsBio) and HRP conjugated goat anti-mouse IgG antibody (AP308P, Sigma) served as primary and secondary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Mouse anti-Ankyrin-G (Neuromab N106/36; IF 1:200) Mouse anti-ß-actin (Sigma A5316; Western blot 1:5000), Rabbit anti-ß-actin (Cell Signaling Technologies #4907 ...
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bioRxiv - Cell Biology 2022Quote: ... The mouse monoclonal anti-β-actin antibody (catalog #: A1978) and mouse monoclonal FLAG antibody (catalog #: F1804) came from Sigma Aldrich. The fluorescent anti-β-actin antibody Rhodamine came from Biorad (catalog # ...
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bioRxiv - Neuroscience 2020Quote: ... then blocked with 3 % BSA in PBS for 1 h at room temperature and incubated with primary antibodies (mouse anti-GFAP, Sigma, 1:500; mouse anti-NeuN, Millipore, 1:500 ...
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bioRxiv - Molecular Biology 2020Quote: ... rabbit anti-phospho-eIF2α (1:1000, CST), mouse anti-eIF2α (1:1000, CST) or mouse anti-GAPDH (1:20000, Millipore) antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit, AB15580, Abcam, 1:250; TuJ1: mouse, MAB1195, R and D Systems, 1:100; GFAP: mouse, clone GA5, MAB3402, Millipore, 1:500 ...
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bioRxiv - Microbiology 2019Quote: ... mouse anti-rabbit IgG antibody:alkaline phosphatase (Sigma-Aldrich; anti-mouse IgG (whole molecule:alkaline phosphatase antibody produced in rabbit (Sigma-Aldrich), and anti-rabbit IgG ...
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bioRxiv - Zoology 2019Quote: ... and then incubated overnight at 4°C in primary antibodies (mouse anti acetylated tubulin, Sigma T6793 or mouse anti tyrosinated tubulin, Sigma T9028 and rabbit anti serotonin ...
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bioRxiv - Zoology 2019Quote: ... and then incubated overnight at 4°C in primary antibodies (mouse anti acetylated tubulin, Sigma T6793 or mouse anti tyrosinated tubulin, Sigma T9028 and rabbit anti serotonin, Sigma S5545 or rabbit anti FMRF-amide ...