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Citations for Millipore Sigma :
2801 - 2850 of 6152 citations for IL 8 CXCL8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... the metabolic activity was quantified using the Cell counting kit-8 (Sigma-Aldrich, cat# 96992), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Media containing viral particles was supplemented with 8 μg/mL Hexadimethrine bromide (Sigma-Aldrich, 107689) to transduce target cells ...
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bioRxiv - Cell Biology 2023Quote: ... 8 explants were transferred to a culture plate insert (Millicell®-CM low height, Millipore) and carefully laid on 2 ml culture medium (DMEM containing 2% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... virus at the MOI of 50 was applied with 8 μg/ml Polybrene (Merck Millipore). Virus-containing medium was replaced with medium containing puromycin (3.5 μg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... K562 cells were transduced with lentivirus in the presence of 8 ug/mL polybrene (Millipore). Medium was replaced after 24 hours ...
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bioRxiv - Plant Biology 2023Quote: ... 8-Hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (HPTS; Sigma; 5mg/ml in 0.4% plant agar) was introduced by performing a minor wound to the epidermis with a fine dental needle 1cm from the root tip ...
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bioRxiv - Systems Biology 2024Quote: 8-well chamber slides were coated with fibronectin in PBS (2.5 µg/cm2, Sigma-Aldrich) for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... Some HCT-8 cell specimens were treated with heparinase II (1.0 U/mL) (Sigma-Aldrich) in RPMI-1640 for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... the metabolic activity was quantified using the Cell counting kit-8 (Sigma-Aldrich, cat# 96992), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... the upper surface of the Transwell filters (8 μm pore size Millipore MultiScreen-MIC plates) was coated with Matrigel as described previously (52) ...
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bioRxiv - Cancer Biology 2023Quote: Cells were seeded in 8-chamber microscopy slides and fixed in 4% formaldehyde (SIGMA, 158127) at 3 ...
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bioRxiv - Neuroscience 2023Quote: ... (4) Negative photoresist SU-8 2000.5 (Kayaku Advanced Materials) was diluted in cyclopentanone (Sigma-Aldrich) at a 4:1 ratio ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were aqueously exposed to 10µM benzo(a)pyrene (Sigma-Aldrich, CAS 50-32-8) in dimethyl sulfoxide (DMSO) ...
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bioRxiv - Cell Biology 2024Quote: ... The obtained cells were cultured with retrovirus and 8 μg/ml polybrane (H9268; Sigma-Aldrich). Uninfected cells were eliminated by puromycin (P8833 ...
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bioRxiv - Cell Biology 2024Quote: ... 200 μL of the virus-containing media and 8 μg/mL polybrene (Sigma-Aldrich, H9268) was added to the cells ...
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bioRxiv - Cell Biology 2024Quote: ... Dilute with ammonium bicarbonate according to checking pH ≥ 8 using pH paper strips (Sigma-Aldrich).
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bioRxiv - Genetics 2024Quote: ... proteins were loaded on an 8% polyacrylamide gel and transferred onto a PVDF membrane (Millipore). Chemiluminescence was induced by Luminata Forte Western HRP Substrate (Millipore) ...
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bioRxiv - Neuroscience 2024Quote: ... antennae were blocked frontally with a drop of eicosane (Sigma Aldrich, CAS: 112-95-8). A small window was opened in the head cuticle ...
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bioRxiv - Neuroscience 2024Quote: ... cells were differentiated on Petri dishes or 8-well Permanox chamber slides (177445, Sigma-Aldrich) precoated with 10 μg/ml solution of laminin 111 (23017–015 ...
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bioRxiv - Molecular Biology 2024Quote: ... 6 or 8 h with 500 µM Indole-3-Acetic Acid (IAA, Sigma-Aldrich #I5148). For induction of Oct4 knockdown in ZHBTc4 cells ...
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bioRxiv - Cell Biology 2024Quote: 1mM Leu-Leu methyl ester hydrobromide (LLOMe) (Sigma-Aldrich, L7393-500MG, CAS: 16689-14-8), 200 μM Chloroquine (Sigma-Aldrich ...
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bioRxiv - Genomics 2024Quote: ... Cells were transduced by plating in viral supernatant with polybrene (8 µg/mL, Sigma-Alrich) to obtain an infection rate of 30%-40% based on GFP measurement by FACS (FACSAria2 ...
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bioRxiv - Neuroscience 2024Quote: ... lentivirus vectors were added in fresh E8 Flex medium containing 8 μg/ml Polybrene (Millipore). The next day (day 0) ...
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bioRxiv - Microbiology 2024Quote: ... The pCDH-lentivirus was used to infect CHO cells with 8 μg/ml Polybrene (Sigma). The transfectants were selected with 5 μg/ml puromycin for 7 days ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were transduced overnight using lentiviral stocks supplemented with 8 μg/ml polybrene (Sigma-Aldrich), after which lentiviral supernatant was replaced by fresh culture medium ...
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bioRxiv - Cancer Biology 2024Quote: ... 8–12-week-old organoids were positioned at the air– liquid interface on Millicell (Millipore) inserts to restrict movement ...
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bioRxiv - Cell Biology 2024Quote: 3D culture cells for 8 days were incubated with collagenase type VII (#C0773; Sigma-Aldrich) at RT for 15 minutes ...
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bioRxiv - Microbiology 2020Quote: ... culture media with serum (DMEM, low glucose, from Sigma-Aldrich; 10% HI-FBS; 1% penicillin-streptomycin solution from Sigma-Aldrich; 1% 200mM L-glutamine from Sigma-Aldrich). All assays were performed with cells in passages 4 to 10.
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bioRxiv - Microbiology 2021Quote: ... from strain BetaCoV/Wuhan/WIV04/2019 was ordered from IDT (Coralville, IA, USA) and cloned into a HIS-SUMO expression vector (a modified pET-DUET; Novagen, Madison, WI, USA). After transformation into BL21(DE3) ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 μM mVP40 proteins or His-tag alone were incubated with 50 μg/ml Ni-NTA-Atto 550 dye (Millipore Sigma, Burlington, MA) in a final volume of 500 μl ...
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bioRxiv - Microbiology 2019Quote: ... The washing step was repeated and 200 µl of a 1:5,000 dilution of the monoclonal Anti-His antibody (Sigma-Aldrich, St. Louis, MO) in PBS + 2% BSA was added to each well ...
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bioRxiv - Cell Biology 2019Quote: ... 20mM reduced glutathione) and analyzed by SDS-PAGE followed by Coomassie staining and immunoblotting with anti-His (dilution: 1:5000; Sigma-Aldrich, Steinheim, Germany), or anti-GFP-antibodies (dilution 1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... and subjected to SDS-PAGE followed by Coomassie Blue staining and Western Blot analysis using an anti-His antibody (Sigma-Aldrich, Darmstadt, Germany) or anti-MYC antibodies (Hruby et al. ...
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bioRxiv - Systems Biology 2021Quote: ... The N-terminal His-tag was digested at room temperature overnight using up to 12 NIH Units per mL of bovine thrombin (Sigma-Aldrich T4648-10KU). Proteins were then purified using size exclusion chromatography (HiLoad 26/600 Superdex 200 pg column from GE Healthcare) ...
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bioRxiv - Biophysics 2021Quote: ... Protein production was confirmed by SDS-PAGE and subsequent Western blotting analysis of a K30 sample after 24 h using an anti-His antibody from mouse (dilution 1:3000, Sigma-Aldrich, cat.no. H1029) and secondary anti-mouse IgG-HRP antibody produced in goat (dilution 1:20,000 ...
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bioRxiv - Plant Biology 2022Quote: ... in the same buffer containing 1/5000 of primary antibody raised against poly-His tag directly coupled with peroxidase (Sigma, catalog no. A7058). After washing 3x by PBS 1x ...
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bioRxiv - Microbiology 2022Quote: ... and then transferred to medium differing by the absence of histidine (his) and the presence of 5 mM 3-amino-1,2,4-triazole (3-AT; Sigma-Aldrich, Saint-Louis, MO, USA). Homologous recombination between pPC86 and the PCR product then reconstituted the expression cassette encoding the AD-fused prey protein ...
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bioRxiv - Plant Biology 2022Quote: ... approximately 100 μg His-IAA17 recombinant proteins were treated with 200 μM GSNO and then labeled with biotin-maleimide (Sigma-Aldrich, Cat #: B1267). After biotinylated proteins were precipitated with cold acetone ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was sequentially incubated at room temperature for one hour with anti-His and conjugated anti-mouse antibodies (Sigma-Aldrich, Darmstadt, Germany) (Wu et al. ...
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bioRxiv - Genetics 2023Quote: ... The samples were collected for a range of incubation times and then examined by western blotting using an anti-His antibody (Sigma, catalogue no. SAB1305538) or anti-GFP (Roche ...
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bioRxiv - Developmental Biology 2021Quote: ... On the day of the experiment cells were treated with 3 ng/ml IL-1α (SRP3310, Millipore Sigma), 30 ng/ml tumor necrosis factor (8902SF ...
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bioRxiv - Microbiology 2020Quote: ... PBMCs were stimulated with 50 U/ml IL-2 and 5◻μg/ml phytohemagglutinin (PHA; Sigma-Aldrich) for 72 h prior to infection or transduction ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then washed twice and resuspended in RPMI complete media containing 40 U/ml IL-2 (Sigma) and cultured for 2 days ...
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bioRxiv - Cancer Biology 2021Quote: ... Nilotinib (AMN107) (Novartis, Basel, Switzerland), and vincristine (Hospira Worldwide Inc., Lake Forest, IL) (Sigma-Aldrich, St. Louis, MO) were dissolved in DMSO and stored at −20°C.
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bioRxiv - Microbiology 2021Quote: ... PBMCs were cultured in 20% FBS-supplemented RPMI media supplemented with 100 ng/mL IL-2 (Sigma, USA) and 50 ng/mL concanavalin A (Sigma ...
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bioRxiv - Immunology 2024Quote: ... anti-IL-12 (RyD Systems) in the presence of 0.25 μg/mL Brefeldin A and 0.0025 mM Monensin (SIGMA) or in combination with anti-MICa/b ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... IL-1β and TNF-α using indirect sandwich enzyme-linked immunosorbent assay (ELISA) (Sigma-Aldrich; #RAB0308 and #RAB0477) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... 10% and 12% SDS-PAGE gel and then transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Kankakee, IL, USA) in the transfer buffer ...
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bioRxiv - Plant Biology 2020Quote: ... the first aliquot was derivatized using cysteamine (0.25 M; pH 8; 1h; room temperature; Sigma-Aldrich), the second aliquot was immediately further processed as following ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein samples were resolved on 8–12% SDS–polyacrylamide gels and transferred to nitrocellulose membranes (Millipore). Subsequently ...