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Citations for Millipore Sigma :
2801 - 2850 of 10000+ citations for 6 Chloro 5 fluoro 1H indole 2 3 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... washed 3 times in 1X PBS and incubated for 5 min in 50mM ammonium chloride (Sigma-Aldrich, 254134) before mounting on cavity blades (Marienfeld ...
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bioRxiv - Molecular Biology 2019Quote: ... To create the Tmem98A193P allele Edit-R crRNA (sequence 5’-CCAAUCACUGUCUGCCGCUG-3’) (Dharmacon) was annealed to tracrRNA (Sigma) in IDT Duplex buffer (IDT) ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were washed with 1X TBS 3 × 5 minutes followed by treatment with 0.05% 3,3’-Diaminobenzidine (Sigma Aldrich) and 0.01% H2O2/1X TBS for 20 minutes ...
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bioRxiv - Biochemistry 2019Quote: ... Dialyzed CTPS2 was then concentration approximately 5-fold using a 3 kDa cut-off centrifugal filter unit (Millipore), flash-frozen in liquid nitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... TACC3 (5’ GAGCGGACCUGUAAAACUA 3’) and a non-targeting oligo against Luciferase was used as control (Sigma, Custom order). RNAi Max (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... thiolated ssDNA oligonucleotide (5’ Thiol-C6-AAT ATC GCG GAC AGA AGA CG 3’) was obtained from Sigma. This sequence was derived from a plasmid of Neisseria gonorrhoeae (pCmGFP ...
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bioRxiv - Microbiology 2021Quote: ... and the sequences of the siRNAs targeting DDX42 were siDDX42-1: 5’-CAGAAUGCCUGGUUUCGGA-3’ (SASI_Hs01_00119846, Sigma-Aldrich®), siDDX42-2 ...
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bioRxiv - Biophysics 2020Quote: ... a mica surface was treated for 5 min with 0.01% (v/v) (3-aminopropyl)triethoxysilane (APTES) (Sigma-Aldrich). HS-AFM observations of the 50S subunits from Pyrococcus furiosus or pre-assembled hybrid 50S subunits were performed as follows ...
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bioRxiv - Physiology 2019Quote: ... ESFs were decidualized by adding of 0.5 mM 8-bromoadenosine 3′,5′-cyclic monophosphate (8-Br-cAMP) (Sigma) and 0.5 μM of the synthetic progestin medroxyprogesterone acetate (MPA ...
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bioRxiv - Genomics 2021Quote: ... Cross-linked chromatin was immunoprecipitated with either 5 μg anti-TCF4/TCF7L2 (Clone 6H5-3, #05-511, Millipore) or normal mouse control IgG (#12-371 ...
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bioRxiv - Bioengineering 2022Quote: For HP-β-CD-treatment 3×SNCA hMOs were exposed to 5 μM HP-β-CD (Sigma Aldrich) in cell culture media starting from D10 of differentiation.
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bioRxiv - Cancer Biology 2022Quote: ... following additional 5 min incubation with 100 nM Anionic Oxonol Dye DiBAC4(3) (D8189, Sigma Aldrich, MO, USA). Fluorescence was analysed on a BD FACSCalibur flow cytometer ...
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bioRxiv - Physiology 2023Quote: ... Samples were separated on a SeQuant ZIC-pHILIC column (100 3 2.1 mm, 5 mm, polymer, Merck-Millipore) including a ZIC-pHILIC guard column (2.1 mm x 20 mm ...
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bioRxiv - Neuroscience 2024Quote: Larval zebrafish were mounted at 5 dpf in 3% low melting point agarose (Sigma-Aldrich, St. Louis, Missouri) on top of a 22x22 mm coverslip ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was then stripped and re-probed with an 18s rRNA targeting probe (5’-CCATCCAATCGGTAGTAGCG-3’, Sigma) which was used for signal normalisation.
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bioRxiv - Neuroscience 2023Quote: ... washed (at 3,724xg at 40C for 3-5 min) (x3) with PBSx1 using Amicon Ultra-15ml (UFC910024 - Millipore), followed by concentration in storage buffer (described in Materials ...
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bioRxiv - Microbiology 2023Quote: ... 3 ×104 THP-1 cells were seeded and differentiated by the addition of 5 ng/ml PMA (Sigma) in complete RPMI media (Himedia ...
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bioRxiv - Developmental Biology 2023Quote: ... Patch pipettes (3-5 mΩ) were filled with potassium gluconate internal solution (130 mM K-gluconate (Sigma-Aldrich), 11.5 mM Na2-phosphocreatine (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2023Quote: ... antisense strand: 5’-GUA UCU CUU CAU AGC CUU A(dT)(dT)-3’) were synthesized by Sigma Aldrich. Early Endosome Antigen 1 (EEA1 ...
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bioRxiv - Cancer Biology 2023Quote: ... fixed with 0.5% glutaraldehyde and incubated for 3 min at RT with 5 mg/ml sodium borohydride (Sigma). After washes ...
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bioRxiv - Cell Biology 2024Quote: ... cells were treated with 10 μM of the agonist N-[(3R)-1-Azabiciclo[2.2.2]oct-3-yl]furo[2,3-c]pyridine-5-carboxamide (PHA-543613, from Sigma, PZ0135) for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... the animal’s drinking water was replaced from days 3-8 with water containing 5% L-arabinose (Sigma Aldrich). InducTn-seq samples were collected 8 days post inoculation by plating fresh feces on solid LB containing 50 µg/ml kanamycin ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 3x PBS washes for 5 minutes at RT before blocking with blocking buffer (BB; 5% normal donkey serum Sigma-Aldrich #S30-M, 3% BSA (Sigma #A4503) and 0.25% Triton-X100 (Sigma #T9284 ...
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bioRxiv - Biophysics 2021Quote: ... The cantilevers were functionalized with amine groups by immersing in 2% (vol/vol) 3-aminopropyltriethoxysilane (Millipore Sigma) solution dissolved in acetone ...
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bioRxiv - Microbiology 2019Quote: Primers used in this study (Table 2) were designed with Primer 3 (http://bioinfo.ut.ee/primer3-0.4.0/) and synthesized by Sigma-Aldrich.
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bioRxiv - Cell Biology 2019Quote: Cell viability was assessed with 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT, #M5655, Sigma Aldrich) after treatment with MMC and the PARP inhibitor Olaparib (AZD2281 ...
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bioRxiv - Bioengineering 2021Quote: ... was added to the PEG solution followed by the addition of 1-[bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxide hexafluorophosphate] (HATU; 2.43 g, 6.4 mmol, 2 eq.; Sigma-Aldrich). Next ...
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bioRxiv - Immunology 2022Quote: ... 2- or 3-days post fertilisation (dpf) zebrafish were anaesthetised in 0.168 mg/ml Tricaine (Sigma-Aldrich) in E3 media and visualised under a dissecting microscope ...
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bioRxiv - Biochemistry 2021Quote: ... cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich, MO) as described previously.52 Equivalent MTT assays were performed on cells cultured in the same ratios of PBS to media ...
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bioRxiv - Microbiology 2021Quote: ... sporozoites were added to 1 ml molten 3% agarose (2-Hydroxyethyl agarose – Sigma-Aldrich, dissolved in ddH2O), centrifuged and incubated at 4°C for 5 min ...
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bioRxiv - Immunology 2021Quote: ... or 25 µM (L-3-trans-(Propylcarbamyl) oxirane-2-carbonyl)-L-isoleucyl-L-proline (CA-074me) (Sigma) to inhibit cathepsin B ...
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bioRxiv - Molecular Biology 2020Quote: ... For cell treatments the following compounds were used: 2′,3′-Dideoxycytidine (1-10 μM, Sigma-Aldrich®), Ethidium bromide (50 ng/mL ...
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bioRxiv - Biochemistry 2022Quote: ... and trans-4-Phenyl-3-buten-2-one (20a) were purchased from Sigma-Aldrich (St. Louis, MO). Ketoisophorone (2a ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and thus 3-[2-(diethylamino)ethyl]-7-hydroxy-4-methylcoumarin (AHMC; Cat. No. 188611, Sigma-Aldrich Corp.) was used instead as previously described for the fluorescence quantification (Donato et al ...
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bioRxiv - Pathology 2019Quote: ... a cell-permeant NO scavenger 2-(4-car-boxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) (Sigma) at a high concentration of 500 μM was included ...
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bioRxiv - Molecular Biology 2020Quote: ... 3 mg total protein were mixed with 2 mL Urea Buffer (UB) (8 M urea (Sigma, U5128) in 0.1 M Tris/HCl pH 8.0 and 50mM ammonium bicarbonate) ...
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bioRxiv - Cancer Biology 2020Quote: ... collected and lysed with NP-40 lysis buffer supplemented with phosphatase inhibitor cocktails 2 and 3 (Sigma) and protease inhibitor mix (Sigma) ...
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bioRxiv - Microbiology 2019Quote: ... 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide was dissolved in Dulbecco’s PBS (-) (Sigma-Aldrich, India) (pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated for 3 hours before addition of 30 μL of the bacterial growth reporter MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (2.5 mg/mL) (Sigma) and Tween 80 (10% ...
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bioRxiv - Biochemistry 2020Quote: ... phosphatidyl-choline (2-oleoyl-1-palmityl-sn-glycero-3-phosphocholine) were from Sigma-Aldrich (St. Louis, MO); Ezetimibe and lysophosphatidylcholine (1-palmitoyl-sn-glycero-3-phosphocholine ...
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bioRxiv - Developmental Biology 2020Quote: ... Phosphopeptide standards (0.1 pmol of MS PhosphoMix 1, 2, 3 Light; Sigma-Aldrich, St. Louis, Missouri, USA) was added to suspended sample in binding/equilibration buffer ...
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bioRxiv - Biochemistry 2021Quote: ... coli polar lipids (Avanti, US) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) (Sigma Aldrich) in a 3:1 molar ratio ...
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bioRxiv - Biochemistry 2021Quote: ... phosphatase inhibitor cocktail 2 and phosphatase inhibitor cocktail 3 were purchased from Sigma-Aldrich (St. Louis, MO). All other chemicals including V8 protease were purchased from FUJIFILM Wako ...
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bioRxiv - Cancer Biology 2021Quote: Cell proliferation was assessed by 3-(4,5-1,2methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Merck Millipore). K562 cells (5,000/well ...
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bioRxiv - Neuroscience 2022Quote: ... The 2 odors used were 3-Oct (Sigma-Aldrich Cat# 218405-250G; CAS Number: 589-98-0) and MCH (Sigma-Aldrich Cat# 66360-250G ...
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bioRxiv - Neuroscience 2022Quote: ... and blocked for 2 hours with blocking buffer (3% donkey serum in 0.1 M PBS; Sigma-Aldrich). Primary antibodies (ChAT ...
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bioRxiv - Neuroscience 2022Quote: ... Internal solutions also contained 3-5mg/mL (0.3%-0.5%) of biocytin (Sigma-Aldrich, CAS# 576-19-2) which was added the day of recording.
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bioRxiv - Systems Biology 2022Quote: ... The virus was collected both 2 days and 3 days later and 0.45 μm syringe filters (Millipore) were used to eliminate cells ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed 3 times in PBS before addition of 2% Triton X–100 (Sigma-Aldrich) PBS solution to lyse the cells for enumeration of the intracellular bacteria ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... phosphatase inhibitor cocktail 2 (P5726) and 3 (P0044) were purchased from Sigma-Aldrich (St. Louis, MO, USA), and Doxorubicin (Adriamycin ...