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Citations for Millipore Sigma :
2801 - 2850 of 10000+ citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... SDS lysate was centrifuged at 20,000 x g for 30 minutes and supernatants were subsequently diluted with 7 volumes of 1.15% Triton-X 100 in TNE with Phosphatase Inhibitor Cocktail 1 and 2 (Sigma) to produce a mixed-micelle lysate with final detergent concentrations of 0.125% SDS and 1% Triton-X 100 ...
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bioRxiv - Neuroscience 2022Quote: ... DNA plasmids were used at 2 µg/µl and mixed with 1% fast green (Sigma-Aldrich, final concentration 0.2%). Plasmids were injected into the ventricle with a pump-controlled micropipette ...
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bioRxiv - Plant Biology 2022Quote: ... were also amplified by PCR using the primers listed in Supplemental Table 2 and cloned into pGEX4T-1 (Novagen) using BamHI/EcoRI sites to N-terminal glutathione S-transferase (GST ...
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bioRxiv - Cell Biology 2022Quote: ... The cell pellet was re-extracted with 450 µL methanol:water (2:1, v/v) containing 1nmol of scyllo-inositol (Sigma) as an internal standard ...
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bioRxiv - Biochemistry 2024Quote: ... homogenised in 6 mL of 2:1 (v/v) chloroform:methanol containing 0.01% (w/v) butylated hydroxytoluene (BHT, Sigma-Aldrich) as antioxidant ...
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bioRxiv - Cancer Biology 2024Quote: ... Lipids were resuspended in chloroform: methanol (2:1 v/v) and loaded onto a silica TLC plate (Sigma 60778). For nonpolar lipid separation ...
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bioRxiv - Neuroscience 2024Quote: Lhx6-Cre;Ppargc1a+/+ and Lhx6-Cre;Ppargc1aF/F animals were injected at P1 with 600nL of custom-made AAV8-hSyn-dio-mito-EmGFP (diluted 1:2 with sterile PBS and 0.5% Fast Green, Sigma). The Vanderhaeghen lab gifted the mito-EmGFP construct ...
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bioRxiv - Neuroscience 2024Quote: ... mixed with AAV8-hSyn-dio-GFP (Addgene #50457-AAV8, 1:2 dilution with sterile PBS and 0.5% Fast Green (Sigma)) ...
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bioRxiv - Neuroscience 2024Quote: ... at a 1:40 dilution for sparse cell targeting and AAV8-hSyn-dio-GFP (Addgene #50457-AAV8, 1:2 dilution with sterile PBS and 0.5% Fast Green, Sigma). The dilution factor of the DREADDs viruses (here 1:40 ...
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bioRxiv - Developmental Biology 2023Quote: ... Secondary antibodies used were as follows: 1:2000 goat anti-mouse F(ab’)2 Alexa488 (Sigma-Aldrich, cat. F8521) and 1:1000 sheep anti-rabbit F(ab’)2 Cy3 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... From this 30ul was used to transduce 0.1 million HUDEP-2 cells taken in 24 well plate with 1% HEPES and 3μg polybrene (Sigma-Aldrich). Spinfection was carried out at 800G for 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% SDS, 1% Triton X-100, 0.5% sodium deoxycholate, 2 mM EDTA, and protease inhibitor cocktail from Sigma Aldrich). Protein content was determined using BCA assay (Life Technologies) ...
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bioRxiv - Physiology 2024Quote: ... washed 2 x 5 min in PBS and then incubated with primary antibodies against Laminin-β1γ1 (1:200; #L9393, Sigma) in 3% BSA in PBS ...
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bioRxiv - Microbiology 2023Quote: The differentiated THP-1 cell lines were seeded into 24-well plates at a density of 1 ×105 cells per well in RPMI-1640 (supplemented with 10% v/v FBS, 1% Penicillin/Streptomycin and 0.05 mM 2-mercaptoethanol) (Sigma) and incubated for 24 hours in 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... The organic fraction was removed from the mixture and diluted 1:2 in 100% ethanol (Sigma cat: E7023-1L). Scanning absorbance values were then taken between 230nm and 290nm on a Nanodrop spectrophotometer ...
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bioRxiv - Microbiology 2023Quote: ... Once the cultures reached OD 0.1 then 108 CFU/ml of each strain was incubated with 10µM of 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Sigma) in 1xPBS (pH 7.2 ...
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bioRxiv - Bioengineering 2023Quote: ... Cell-laden hydrogels were cut into smaller pieces and cultured in growth medium supplemented with 0.05 mg ml-1 L-ascorbic acid-2-phosphate (Sigma) and 1 mM calcium chloride (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were resuspended in 150 µl of FACS buffer (PBS supplemented with 0.5 % fetal bovine serum and 2 mM EDTA) containing DAPI (1:50,000, Sigma-Aldrich) as previously described (Fischer et al ...
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bioRxiv - Cell Biology 2023Quote: ... buffered with 0.15 mM HEPES (pH = 7.6) and supplemented with 200 mM of 1-Phenyl-2-thiourea (Sigma-Aldrich) to inhibit the melanogenesis ...
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bioRxiv - Bioengineering 2023Quote: ... Cell-laden were cut into 6x6 mm squares and cultured in growth medium supplemented with 0.05 mg ml-1 L-ascorbic acid-2-phosphate (Sigma) and 1 mM calcium chloride (Sigma ...
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bioRxiv - Immunology 2022Quote: ... samples were re-loaded four times before washing with 1 mL of 2% acetonitrile (ACN) (#1000292500, Sigma-Aldrich, Merck) in 0.2% acetic acid ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 μL droplets containing a total of 1000 cancer and stellate cells in a 1:2 ratio were prepared in a 0.24% solution of methylcellulose (M0512, Sigma) and plated on the underside of a tissue culture plate lid ...
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bioRxiv - Immunology 2022Quote: ... and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma)) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: Recordings were then performed in oxygenated aCSF superfused at a rate of 2-3 ml/min and supplemented with 100 µM picrotoxin (Alomone Labs) and 0.5-1 µM Strychnine (Sigma). The internal solution was the same as above with 2-4 mM QX314 to block Na+ channels from driving AP firing ...
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bioRxiv - Molecular Biology 2022Quote: ... and pellets were resuspended in NP40 buffer (20 mM Tris pH 7.5, 150 mM NaCl, 2 mM EDTA, 1% NP40) and treated with Benzonase (Sigma). Anti-HA magnetic beads (Pierce ...
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bioRxiv - Neuroscience 2022Quote: ... and 80% Buffer-Z (0.1 M Na2HPO4, 10 mM KCl, 1 mM MgSO4, and 3.4 µL/mL 2-mercaptoethanol; all from Sigma-Aldrich). Luminescence and absorbance (410 nm ...
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bioRxiv - Plant Biology 2022Quote: ... as well as commercially available mouse anti-alpha-tubulin (Sigma-Aldrich, USA, cat. no. T9026-2, dilution 1:300), rabbit anti-histone H3K4me2 (abcam ...
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bioRxiv - Biochemistry 2023Quote: ... pH 7.4) and adjusted to an OD600 of 1.0 in HEPES buffer containing either 1 mM Aldrithriol-2 (Sigma-Aldrich, Darmstadt ...
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bioRxiv - Bioengineering 2022Quote: A mixed solution containing 2 μL/mL of Ethd-1 and 0.5 μL/mL of Calcein AM (Sigma-Aldrich) was prepared ...
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bioRxiv - Developmental Biology 2023Quote: ... The dried residues were reconstituted in 50 µL of 2% MeOX in anhydrous pyridine (110-86-1, Sigma-Aldrich) followed by derivatization at 70°C for 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... HUVEC or HUAEC cells were transfected with the above lentiviral supernatants diluted (2:1) with ECMB supplemented media with polybrene (8μg/ml) (Sigma) overnight ...
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bioRxiv - Biophysics 2023Quote: ... blocked and stained with anti-alpha tubulin primary antibodies (monoclonal mouse clones B-5-1-2 and DM1a, Sigma) overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... consisting of 1% FBS and gluMax along with 5 μM CHIR99021 and 2 μM retinoic acid (R2625, Sigma-Aldrich) for 72 hrs ...
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bioRxiv - Developmental Biology 2024Quote: ... pCAG-PiggyBac transposase or PB-pCAG-H2B-GFP (final concentration: 1-2 μg/μL) plasmid was mixed with 0.05% Fast Green (Sigma) and injected into the lateral ventricle of embryos (0.5 μL per embryo ...
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bioRxiv - Evolutionary Biology 2024Quote: Tissue was minced with a scalpel into ∼1 mm3 cubes in 2 mL of digestive solution containing 0.2 mg/mL Liberase TL (05401020001, Sigma). Tissue suspensions were heated at 37°C for 15 minutes and then passed 10 times through a 16-gauge needle attached to a 3-mL syringe ...
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bioRxiv - Molecular Biology 2024Quote: ... 1% yeast extract) and in synthetic complete (SC) medium (2% glucose, 0.67% yeast nitrogen base without amino acids (Sigma), 0.2% synthetic drop-out supplement (Sigma)) ...
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bioRxiv - Molecular Biology 2024Quote: ... Pellets were resuspended in NP40 buffer (20 mM Tris pH 7.5, 150 mM NaCl, 2 mM EDTA, 1% NP40) and treated with Benzonase (Sigma) as described in 21 ...
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bioRxiv - Plant Biology 2024Quote: ... 2 μCi [γ-33P] ATP) with 1 µg substrate (purified GST-NLP7 or myelin basic protein (MBP, Sigma M1891)) at room temperature for 30 min ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were diluted to 100 µg/L in 1× TE buffer with 2 g/L heparin (Sigma-Aldrich, U.K.). Standard solutions from the corresponding RNA stock were also prepared in a 1× TE buffer with 2 g/L heparin to account for any variation in fluorescence ...
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bioRxiv - Plant Biology 2024Quote: ... 50 mM Tris-acetate pH7.5, 2 mM EGTA-Tris, 1% PVP-40, 2mM DTT, 0.5×protease inhibitor cocktail [Sigma-Aldrich]). The protein concentration was determined using a Bio-Rad Bradford protein assay kit (Bio-Rad) ...
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bioRxiv - Bioengineering 2024Quote: ... and chips were blocked and permeabilized for 2 hours by adding PBS containing 1% Triton X-100 (Sigma #T8787) and 3% bovine serum albumin (BSA ...
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bioRxiv - Plant Biology 2024Quote: ... roots underwent a 2-min treatment with 1% bleach followed by immersion in boiling acid fuchsin solution (Sigma-Aldrich) for 2 to 3 min ...
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bioRxiv - Biochemistry 2024Quote: Cells were collected and resuspended in lysis/IP buffer (50 mM HEPES pH 7.4, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 2x cOmplete protease inhibitor tablet [5056489001, SIGMA]) or IP Buffer with 0.5 mM DTT ...
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bioRxiv - Genetics 2024Quote: ... cells were washed with PBS and pellets were resuspended in 350 µl of RLT + 1% 2-Mercaptoethanol (Sigma Aldrich) and stored at −80 °C.
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bioRxiv - Molecular Biology 2024Quote: ... we grew cells in media supplemented with 1 mM or 2 mM of the drug BIX01294 G9a inhibitor (Sigma). For passaging ...
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bioRxiv - Microbiology 2024Quote: ... 20 U/mL IL-2 (AIDS Reagent Program, Germantown, MD) and 1% penicillin/streptomycin (Sigma- Aldrich, St. Louis, MO), Cells were activated for 3 days with 1 μg/mL/million cells of purified NA/LE mouse anti-human CD3 and CD28 (BD Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... 20-5310-05) (2 washes at 1 min each) followed by permeabilization using protease (0.34 µg/ml) (Sigma, P5380) in 1x PBS for 7.5 min ...
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bioRxiv - Cell Biology 2024Quote: ... the beads with bound lysosomes were resuspended in 80 µl ice-chilled 1x extraction buffer (50 mM HEPES at pH 7.4, 40 mM NaCl, and 2 mM EDTA, 1% Triton X-100 (Sigma), 1.5 mM sodium orthovanadate (NaVO4) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 4-OHT was washed off at 30 hpf and replaced by E3 medium containing 0.003% 1-phenyl-2-thiourea (PTU, Cat#P7629; Sigma) to prevent the formation of pigmentation ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The cell pellets were resuspended in 2 mL lyticase buffer (1 M sorbitol, 0.1 M EDTA, 0.1% β-mercaptoethanol) plus 50 U of lyticase (Sigma) and incubated for 30 min at 30°C ...