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Citations for Millipore Sigma :
2751 - 2800 of 4323 citations for Recombinant Human VWC2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: TKO and QKO mESC were cultured in Serum/LIF media (DMEM-KO, 15% FBS, 1000 U/ml murine recombinant LIF (Millipore Sigma #ESG1106), 1× nonessential amino acids (100x ...
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bioRxiv - Developmental Biology 2024Quote: ... Serial dilutions from 2μg to 0.0625μg of recombinant SPARC (SRP3159, Sigma-Aldrich; S5174-25UG, Sigma-Aldrich; 942-SP-050, R&D Systems) were spotted and used as standards ...
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bioRxiv - Biochemistry 2023Quote: We performed the phosphorylation kinetics presented in Figure 4a using commercial recombinant kinases GST-p38βat 10 μg/mL (Sigma-Aldrich, ref. B4437), GST-Erk2 at 20 μg/mL (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products from each targeted exon were purified and mixed with 0.5 units of recombinant spCas9-GFP (Sigma-Aldrich, Cat # CAS9GPRO), 10 μM gRNA in 2 μL of 10x Cas9 reaction buffer ...
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bioRxiv - Microbiology 2023Quote: ... were transformed with the lithium acetate method 45 and strains containing the recombinant plasmids were selected on YPD agar with 200 μg/mL geneticin (G418 – Sigma A1720-5G).
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bioRxiv - Cell Biology 2023Quote: The custom recombinant antibody-like anti-MAR binding reagent (anti-MAR) was generated and purified in-house (now available from Millipore Sigma, MABE1076) (Gibson et al. ...
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bioRxiv - Cell Biology 2024Quote: ... The recombinant protein from the cell lysate was purified using Glutathione Sepharose® 4B GST affinity columns (Millpore Sigma, GE17-0756-05). The purified PCM1-GST fusion protein was analyzed on SDS-PAGE stained with Coomassie Blue (Invitrogen) ...
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bioRxiv - Genetics 2021Quote: ... Anti-His (SCBT, #sc-8036; used in 1:2000), anti-GST (SCBT, #459; used in 1:5000) and anti-Flag (Sigma-Aldrich, #F7425;used in 1:2000). Peroxidase conjugated anti-mouse (Jackson Immunoresearch ...
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bioRxiv - Cell Biology 2019Quote: ... Protein eluates were separated by SDS PAGE and stained with Coomassie Brilliant Blue or with anti-His antibody after transfer onto a nitrocellulose membrane (Sigma-Aldrich, Steinheim, Germany; dilution 1: 5000).
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bioRxiv - Cell Biology 2019Quote: ... The proteins of the eluate were separated by SDS PAGE and stained with Coomassie Brilliant Blue or with anti-His antibody after transfer onto nitrocellulose membrane (Sigma-Aldrich, Steinheim, Germany; dilution 1: 5000).
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bioRxiv - Cell Biology 2020Quote: A2780 human ovarian cancer cells were maintained in RPMI-1640 medium (Sigma-Aldrich) supplemented with 10% (v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-human α-smooth muscle actin (Cy3 conjugated, cat. #C6198, Sigma Aldrich, 1:500 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then suspended in veronal buffer and human serum (Sigma-Aldrich, H4522) (or heat-inactivated for 30min at 56°C) ...
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bioRxiv - Molecular Biology 2021Quote: The gene of human GAPDH was cloned into pET-28a(+) expression vector (Novagen) with an N-terminal His-tag followed by the Tobacco Etch Virus (TEV ...
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bioRxiv - Biochemistry 2022Quote: ... or −3 human cDNA into a p3XFLAG-CMV-14 expression vector (Sigma, E7908). Then 3XFLAG-PRLs were cloned into pLenti-CMV-puro (Addgene 17452 ...
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bioRxiv - Cell Biology 2022Quote: ... dried and incubated with 10 μg/mL of IgG from human serum (Sigma) in PBS for 1 hour at RT ...
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bioRxiv - Neuroscience 2021Quote: ... were injected with 400 IU human chorionic gonadotropin (hCG; Sigma-Aldrich CG10; RRID:SCR_018232).
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bioRxiv - Molecular Biology 2021Quote: ... bleomycin and human activating FAS (anti-FAS) antibody were purchased from Millipore Sigma. PGE2 ...
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bioRxiv - Immunology 2022Quote: ... media was replenished with RPMI containing 5% type AB human serum (Sigma-Aldrich). For single-round experiments with VSV-G-pseudotyped viruses ...
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bioRxiv - Systems Biology 2019Quote: ... The human plasma reference standard NIST SRM 1950 was obtained from Sigma Aldrich. Human cancer cells (HeLa S3 ...
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bioRxiv - Cell Biology 2020Quote: ... in acetic acid (0.02 N) or human plasma fibronectin (5 µg/mL; Millipore) in PBS overnight at 4°C and rinsed with imaging medium to remove the coating solution ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were blocked with human IgG (1.25 mg/mL, 15 min; Sigma Aldrich). Surface staining of unfixed/unpermeabilized cells employed the following antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... PINK1 knockdown was performed using MISSION® esiRNA human PINK1 (EHU057101, Sigma Aldrich). Mff knockdown (SI Fig ...
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... Sections were incubated with rabbit anti-human-IgG (1:100, Sigma, SAB3701291, IgG) and with rabbit anti-human IgM (1:250 ...
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bioRxiv - Immunology 2019Quote: ... anti-human C5aR or nonpeptide C5aR antagonist W-54011 (5 μM, Merck Millipore)(25 ...
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bioRxiv - Cell Biology 2019Quote: ... Staining for murine and human MSCs was performed using Oil Red O (Sigma) stain for adipogenesis ...
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bioRxiv - Cell Biology 2020Quote: The human ovarian cancer cell lines COV318 (purchased from ECACC, via Sigma-Aldrich) and SKOV3 (courtesy of Dr Florence Delie ...
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bioRxiv - Cancer Biology 2019Quote: ... human breast cancer were detached using Trypsin (when grown as monolayer, Sigma-Aldrich) or pelleted and dissociated using StemPro Accutase (when grown as spheres ...
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bioRxiv - Immunology 2021Quote: ... Total IgA purified from human serum was used as a standard (Sigma-Aldrich). To generate the IgA standard curve anti-human IgA capture antibodies ...
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bioRxiv - Immunology 2021Quote: ... and then overlaid with complete DMEM containing 10 μg/mL human IgG (Sigma). 48 hours post infection ...
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bioRxiv - Microbiology 2021Quote: ... Primary human monocytes were treated with 50 ng/ml M-CSF (Sigma-Aldrich) in complete RPMI for a week before infection ...
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bioRxiv - Cancer Biology 2021Quote: MISSION shRNA targeting mouse or human PSME4 or RFP were obtained from Sigma (TRCN0000176569 ...
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bioRxiv - Bioengineering 2021Quote: ... and human β2m were purchased from IDT and cloned into pET3a vectors (Novagen). H2-Db and H2-Kb genes were engineered with a C-terminal cysteine by site-directed mutagenesis (NEB) ...
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bioRxiv - Bioengineering 2021Quote: Human serum albumin (> 97% lyophilized) was purchased from Sigma Aldrich (Oakville, ON, Canada). Glutaraldehyde (25% aq ...
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bioRxiv - Bioengineering 2021Quote: ... Serum pramlintide concentrations were quantified using a human amylin ELISA kit (Millipore Sigma). Serum lispro concentrations were quantified using Northern Lights Mercodia Lispro NL-ELISA ...
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bioRxiv - Bioengineering 2021Quote: ... 100 ng/mL Follicle Stimulating Hormone from human pituitary (FSH, Millipore Sigma, F4021), 10 ng/mL LH ...
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bioRxiv - Biochemistry 2021Quote: FFPE human tonsils were deparaffinized in xylene substitute (Sigma-Aldrich, St. Louis, MO), rehydrated in a graded ethanol series and stained for 10 minutes with Atto 465-carboxcylic acid (8 uM ...
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bioRxiv - Biophysics 2021Quote: ... lyophilized rabbit muscle aldolase and lyophilized human hemoglobin were purchased from Sigma Aldrich. The catalase was diluted to a final concentration of 2.3 mg ml-1 in the buffer of PBS (pH 6.5) ...
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bioRxiv - Neuroscience 2021Quote: ... were injected with 400 IU human chorionic gonadotropin (hCG; Sigma-Aldrich CG10; RRID:SCR_018232). The following day ...
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bioRxiv - Cancer Biology 2021Quote: siRNAs targeting human SUV39H1/H2 and negative control siRNAs72 were from Sigma-Aldrich. Transfections were performed using Lipofectamine RNAiMax (Thermo Fisher ...
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bioRxiv - Biophysics 2021Quote: ... pre-coated with 30 μg/ml of fibronectin from human plasma (Sigma-Aldrich) and nanodiamonds (100 nm ...
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bioRxiv - Neuroscience 2020Quote: ... Plates were incubated with anti-human Fab specific-HRP conjugated secondary antibody (Sigma) for 1 h at RT ...
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bioRxiv - Bioengineering 2021Quote: ... a standard curve was obtained by diluting human Hgb powder (Sigma-Aldrich, USA) in human plasma (Cambridge Bioscience ...
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bioRxiv - Microbiology 2021Quote: Human umbilical VECs (Cell Application 200-05n) were cultured on fibronectin (Sigma 10838039001)-coated glass slides or polyacrylamide (PA ...
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in PBS with 2% Human Heat Inactivated AB Serum (Sigma) and 0.1 M EDTA pH 8.0 (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: Cells were treated with the indicated concentrations of DLK1 human (Sigma Cat# SRP8006) or Recombinant Human DLK-1 protein (Abcam Cat# ab151926) ...
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bioRxiv - Cell Biology 2021Quote: ... 350 μl of 1:25 diluted 0.1% human serum derived fibronectin (Sigma-Aldrich) was added per stamp and incubated at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... human β2 adrenergic receptor (β2ADR) with isoproterenol (Iso; Sigma-Aldrich I5627, in water) Epinephrine (Sigma-Aldrich E4642 ...
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bioRxiv - Cancer Biology 2021Quote: Organoids and human tissue were fixed with 3.7% paraformaldehyde (PFA, Sigma-Aldrich, # 4441244), dehydrated ...
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bioRxiv - Molecular Biology 2022Quote: ... penicillin-streptomycin (Thermo 15140122) and 10 ug/mL human transferrin (Sigma-Aldrich T8158). HCA-7 cells were maintained in a 1:1 mix of DMEM and F12 (Thermo 11320033 ...