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Citations for Millipore Sigma :
2751 - 2800 of 10000+ citations for Recombinant Human BDH2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: RNA-Binding protein immunoprecipitation (RIP) for PCBP1 was perform using Magna RIP RNA-binding protein immunoprecipitation kit (Millipore, cat 17-700) and the same PCBP1 antibody used for eCLIP-seq (Abcam cat# ab168377) ...
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Immunogenic fusion proteins induce neutralizing SARS-CoV-2 antibodies in the serum and milk of sheepbioRxiv - Bioengineering 2022Quote: Antigen functional activity was confirmed by assessing ACE2 binding relative to a spike protein positive control (Callaghan Innovation, New Zealand) and ovalbumin negative control protein (Sigma, #A5503). ELISA plates were coated for 1 hour by incubation at ambient temperature with 50 μL antigen (0.072 nM ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were mixed with 200uL of pre-washed slurry of a 1:1 mix of EZview Red Protein G and EZview Red Protein A bead affinity gel slurry (Sigma-Aldrich) and rotated at 4C for 1hr ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was eluted with 4 mL of elution buffer and concentrated to 1 mL in a 3K protein concentrator (Millipore Sigma). Concentrated protein was purified by SEC as described above.
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bioRxiv - Cell Biology 2024Quote: ... Pelleted debris was discarded and supernatants (solubilized mitochondrial proteins or protein complexes) were transferred to fresh tubes and coomasie brilliant blue G (CBBG, Sigma B0770) was added from a 5% stock to give a 4 g:g (digitonin:CBBG ...
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bioRxiv - Microbiology 2024Quote: ... at low voltage (50 V) and constant current in a 4°C until the protein marker (BLUeye Prestained Protein Ladder, Sigma-Aldrich) front reached the end of the gel ...
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bioRxiv - Microbiology 2020Quote: ... bacteria were incubated with 0.5 µg/mL of chicken anti-human C1qA (Sigma-Aldrich), followed by incubation with PE-conjugated donkey F(ab’)2 anti-chicken (Jackson ImmunoResearch) ...
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bioRxiv - Immunology 2021Quote: ... 0.4µg pCMV-VSV-G and 1.6µg of a lentiviral vector (for human shSUV39H1, Sigma, TRCN0000157251 ...
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bioRxiv - Developmental Biology 2021Quote: ... were soaked overnight with human SDF-1 3.3 μg ml−1 (Sigma-Aldrich, S1577) at 4 °C ...
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bioRxiv - Genomics 2021Quote: ... Human HEK293T embryonic kidney cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2021Quote: Human neutrophils were purified from fresh peripheral blood by Histopaque®-1119 (Millipore Sigma) and subsequent discontinuous Percoll® gradient centrifugation (102) ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated with primary antibodies (mouse anti-human synuclein, Sigma S5566 (clone SYN211) for human alpha-synuclein ...
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bioRxiv - Immunology 2022Quote: Human monocytes from 3 donors were culture in 8-well chamber slides (Millipore-Sigma) in SFM ...
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bioRxiv - Immunology 2022Quote: Human monocytes from 3 donors were culture in 8-well chamber slides (Millipore-Sigma) in SFM ...
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bioRxiv - Immunology 2022Quote: Human monocytes from 3 donors were cultured in 8-well chamber slides (Millipore-Sigma) in SFM ...
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bioRxiv - Immunology 2022Quote: Human monocytes from 3 donors were cultured in 8-well chamber slides (Millipore-Sigma) in SFM ...
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bioRxiv - Biochemistry 2020Quote: ... full length human PCNA (accession no. NM182649) was cloned into pETDuet-1 MCS1 (Novagen) Amp+ to obtain 6× His N-terminally tagged protein ...
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bioRxiv - Developmental Biology 2019Quote: ... S4 are anti human Tcf7l2 (N-20, SCBT) and anti-gamma tubulin (T9026, Sigma) HRP coupled secondary antibodies (1/2,000 ...
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bioRxiv - Genomics 2019Quote: ... RNA was extracted from human islets using Trizol (Ambion, UK or Sigma Aldrich, Canada). To clean remaining media from the islets ...
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bioRxiv - Immunology 2019Quote: ... incubated with alkaline phosphatase-conjugated goat anti-human IgG (1 μg/mL, Sigma-Aldrich) for 1 hr at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... Monocyte-derived macrophages were grown in RPMI 1640 supplemented with 10% human serum (Sigma) with added L-Glutamine ...
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bioRxiv - Developmental Biology 2019Quote: ... Human cells were detected using directly Cy3-conjugated anti-HNA (Millipore, MAB1281C3, 1:100). After washing in TBS ...
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bioRxiv - Pathology 2019Quote: ... CG (22 ng/μL in PBS) was isolated from human leukocytes (Sigma-Aldrich, Germany). Purified native lubricin (SF Lub ...
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bioRxiv - Immunology 2020Quote: ... pMCPECs media supplemented with 5μg/ml human insulin (Sigma Aldrich, St Louis, MO, USA), 10ng/ml hEGF (Peprotech ...
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bioRxiv - Molecular Biology 2019Quote: RAW264.7 mouse macrophage or C32 human melanoma cells were maintained in DMEM (Sigma-Aldrich) supplemented with 10% fetal bovine serum ...
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bioRxiv - Immunology 2019Quote: Human cytokine quantification was performed using a custom 31-plex Luminex panel (EMD Millipore) containing the following analytes ...
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bioRxiv - Immunology 2019Quote: ... wells were incubated with HRP-conjugated anti-human IgG or IgM antibodies (Sigma-Aldrich) diluted 1:500 in PBS/BSA for 90 min at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... and DENV-specific antibody binding quantified using anti-human IgG HRP (Sigma-Aldrich, SAB3701362). Secondary antibody binding was quantified using the TMB Microwell Peroxidase Substrate System (KPL ...
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bioRxiv - Pathology 2021Quote: Cell culture inserts were coated with human placental type IV collagen (Sigma-Aldrich, C7521). CRC expanded cells were seeded at 2.5 × 105 cells on 12-mm Snapwells (Corning Costar ...
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bioRxiv - Developmental Biology 2021Quote: ... they underwent intraperitoneal injection of 20 IU human chorionic gonadotropin (9002-61-3, Sigma) (27) ...
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bioRxiv - Developmental Biology 2021Quote: ... and seeding on 16.7 µg/ml human plasma fibronectin-coated plates (Merck Millipore, FC010). After 48h ...
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bioRxiv - Immunology 2021Quote: ... human endometrial cells were incubated for 4 hours with monensin and Brefeldin A (Sigma) in the presence or absence of 100 ng/ml LPS (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... and DENV-specific antibody binding quantified using anti-human IgG HRP (Sigma-Aldrich, SAB3701362). Secondary antibody binding was quantified using the TMB Microwell Peroxidase Substrate System (KPL ...
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bioRxiv - Microbiology 2021Quote: ... and differentiated for one week in RPMI containing 10% human AB serum (H4522, Sigma), GlutaMAX ...
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bioRxiv - Bioengineering 2020Quote: ... anti-human mitochondria antibody Alexa Fluor® 488 conjugate (MAB1273A4, 1:100, EMD Millipore), and DAPI.
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The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: Co-immunoprecipitation experiments were performed using human kidney HEK293T cells provided by (Sigma-Aldrich). Cells were plated in a 1 ml well microplate and transfected at 70-80% confluence using Lipofectamine 2000 following the manufacturer protocols (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... MaxiSorp plates (Thermo) were coated with 100µl of goat anti-human Fc antibody (Sigma) diluted 1:500 in PBS overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed before detection and goat anti-human Fc-HRP conjugated antibody (Sigma) (1:5000 dilution in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... then resuspended in 1:500 anti-human IgG antibody conjugated to Cy3 (Sigma #C2571). Fluorescence staining was measured by a BD Fortessa flow cytometer.
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bioRxiv - Microbiology 2020Quote: ... 5 μg human insulin mL-1 and 5×10-7 M hydrocortisone hemisuccinate (Sigma). Cells were seeded and expanded for two weeks and subsequently differentiated for another two weeks in the presence of 1.8% DMSO61 (dHepaRG).
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bioRxiv - Microbiology 2020Quote: ... 5 μg human insulin mL−1 and 5×10−7 M hydrocortisone hemisuccinate (Sigma). As a control we generated HepaRG cells expressing an inactive HBx null mutant (HepaRG-HBxSTOP ...
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bioRxiv - Neuroscience 2020Quote: ... The human neuroblastoma cell line SH-SY5Y was obtained from Sigma (Cat no. 94030304) at passage number 5 and used for generation of all other CRISPR-Cas9 edited cell lines ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were treated with 500 ng/ml human anti-Fas (Millipore Sigma, Clone ZB4) with and without 1000 ng/ml of TRAIL ...
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bioRxiv - Cell Biology 2021Quote: ... dishes and flasks were coated in 10 μg/ml human plasma fibronectin (FN) (Millipore) overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... the human cTnC gene (TNNC1) within the pET-21a(+) vector was ordered from Novagen and the Phusion site directed mutagenesis kit (Thermo ...
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bioRxiv - Neuroscience 2022Quote: ... The following synaptic antibodies were used: Rabbit anti-human SYNAPSIN1 (1:1000, Millipore, AB1543), Chicken anti-human MAP2 (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: The human tongue OSCC lines (H357, SCC4 and SCC9) were obtained from Sigma Aldrich, sourced from European collection of authenticated cell culture ...
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bioRxiv - Cancer Biology 2022Quote: ... 50,000 human ECs (RBPJ knock-out or shRNA for CXCL2 (pLKO.1; Sigma Aldrich) and respective controls ...
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bioRxiv - Microbiology 2022Quote: ... Plates with Towne virus were stained with mouse anti-human CMV IE1 (MAB810, Millipore) then goat anti-mouse IgG-AF488 (Millipore) ...
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bioRxiv - Developmental Biology 2022Quote: ... followed 48 hours later by 5 IU of human Chorionic Gonadotropin (hCG) (Sigma-Aldrich). In order to avoid handling-related changes in cortical ER cluster morphology ...