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Citations for Millipore Sigma :
2751 - 2800 of 7654 citations for Parvovirus VP2 Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Separated proteins were transferred to PVDF Immobilon-FL membranes (Millipore, Billerica, MA) using the BioRad Trans-Blot Turbo Transfer System (Hercules ...
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bioRxiv - Neuroscience 2021Quote: ... immunoreactive proteins were visualized using Immobilon® Classico Western HRP Substrate (Millipore) or Immobilon® Forte Western HRP Substrate (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... Protein was reduced with tris (2-carboxyethyl) phosphine (Sigma-Aldrich, MO, USA) and alkylated with methylmethanethiosulphonate (MMTS ...
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bioRxiv - Physiology 2021Quote: ... All images were normalized to Amido Black total protein stain (A8181, Sigma) using the entire lane corresponding to each sample.
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bioRxiv - Biochemistry 2021Quote: ... Protein solution (4 mg/mL in 50 mM Tris-HCl (Sigma-Aldrich), pH 8.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich) and purified with a Superdex 200 pg (Cytiva ...
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bioRxiv - Neuroscience 2020Quote: ... the protein samples were reduced with 20 mmol/L dithiothreitol (DTT, Sigma) at 37 °C for 1 h and alkylated with 50 mmol/L iodoacetamide (IAA ...
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bioRxiv - Microbiology 2021Quote: ... or thrombin protease (10 U/mg of fusion protein, Sigma-Aldrich, Germany). Importin β1 ...
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bioRxiv - Microbiology 2021Quote: Immunoprecipitation was conducted using PureProteomeTM Protein A/G Mix Magnetic Beads (Millipore). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were transferred to polyvinyl difluoride membranes (Immobilon-P, #IPVH00010, Millipore/Sigma) and incubated overnight at 4 °C with the primary anti-Plin2 antibody (ADFP rabbit polyclonal antibody ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were transferred to polyvinyl difluoride membranes (Immobilon-P, #IPVH00010, Millipore/Sigma) and incubated overnight at 4 °C with the primary anti-Plin2 antibody (ADFP rabbit polyclonal antibody ...
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bioRxiv - Developmental Biology 2022Quote: ... Subsequently protein thiol groups were blocked with 10mM iodoacetamide (Sigma-Aldrich, USA) at RT for 45 min ...
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bioRxiv - Genomics 2022Quote: Fusion protein overexpression was induced by treatment with doxycycline (dox) (Sigma Aldrich) at a concentration of 1µg/mL for the specified amount of time ...
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bioRxiv - Biochemistry 2022Quote: ... All reagents used for protein purification and enzymatic assays were from Sigma.
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bioRxiv - Neuroscience 2022Quote: ... protein content was additionally measured using the Bradford assay (Sigma-Aldrich, USA). Bovine serum albumin dissolved in 1xPBS was used for the standard curve ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Proteins were probed with rabbit anti-HA (1:1,000 Sigma-Aldrich, H3663) or anti-β-actin as loading control (1:10,000 Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Proteins were probed with mouse anti-FLAG (1:5,000 Sigma-Aldrich, F3165) or anti-β-actin (1:5,000 Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... mice received either 5 nmol Endo Grade® Ovalbumin protein (Sigma-Aldrich) alone ...
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bioRxiv - Immunology 2022Quote: ... proteins were transferred to a methanol-activated PVDF-FL membrane (Millipore Sigma) in NuPAGE transfer buffer (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... and fibrinogen from bovine plasma (Type I-S, ≥75% clottable protein, Sigma). A 6-8 wt% gelatin stock solution was made by dissolving lyophilized gelatin in calcium and magnesium-free phosphate-buffered saline (10 mM PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Immunohistochemistry was performed using glial fibrillary acidic protein (GFAP) (1:1000; Millipore) and ionized Ca2+-binding adapter protein (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred onto methanol-activated Immobilon-P PVDF membranes (Merk Millipore) using a Mini-Transblot Cell (Bio-Rad) ...
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bioRxiv - Biochemistry 2019Quote: ... Selenomethionine substituted proteins were overexpressed in Escherichia coli B834 (DE3) cells (Novagen) using PASM-5052 autoinduction media (Studier 2005) ...
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bioRxiv - Immunology 2019Quote: ... Rabbit anti-Myc Abs and Protein A-HRP were purchased from Sigma and Southern Biotech ...
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bioRxiv - Biochemistry 2019Quote: ... The protein samples were reduced with 20 mmol/L dithiothreitol (DTT, Sigma) at 37 °C for 1 h and then added to 50 mmol/L iodoacetamide (IAA ...
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bioRxiv - Neuroscience 2019Quote: ... The separated proteins were transferred onto Immobilin-FL PVDF membrane (EMD Millipore), blocked for 1 hour with Odyssey blocking buffer (LICOR ...
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bioRxiv - Cell Biology 2019Quote: Proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes (Millipore). Membranes were blocked with LiCor blocking buffer and incubated with the indicated antibodies ...
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bioRxiv - Molecular Biology 2019Quote: ... supernatant was incubated with protein A+G magnetic beads (Millipore 16-663) for 1h for preclearing at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... Then the proteins were transferred onto a polyvinylidene fluoride (PVDF) membrane (Millipore) using a Mini Trans Blot system (Bio-Rad) ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein content of lysed samples was quantified using BCA assay (Sigma-Aldrich). MILLIPLEX MAP Akt/mTOR phosphoprotein kit ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were probed with rabbit anti-human Cx43 (1:2000) (Sigma-Aldrich) and rabbit anti-ß-Tubulin antibodies (1:2000 ...
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bioRxiv - Microbiology 2019Quote: ... HA-tagged proteins were recognized using a murine antiHA antibody (Sigma-Aldrich) and then an antiMouse IgG-peroxydase antibody (KPL) ...
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bioRxiv - Neuroscience 2019Quote: ... Protein was then transferred to Immobilon-P PVDF membranes (Millipore, Bedford, MA) using a semi-dry transfer apparatus (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2020Quote: ... The purified proteins were concentrated using Amicon concentrators (100-kDa cutoff; Millipore), aliquoted ...
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bioRxiv - Biochemistry 2020Quote: ... The purified proteins were concentrated using Amicon concentrators (100-kDa cutoff; Millipore), aliquoted ...
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bioRxiv - Biophysics 2019Quote: ... All proteins were expressed in Rosetta (DE3) plyS cells (Novagen #70956-3) in an LB medium ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Protein samples were then cleaned using 10 kDa Amicon Filter (UFC501096, Millipore) three times using 9M urea and two times using MyPro-Buffer 1 (Omics Technologies ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were scanned and protein bands were normalized to GAPDH (Millipore, #MAB374). The relative band intensity was determined using Image-J ...
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bioRxiv - Genomics 2019Quote: ... We transferred proteins to an Immobilon-PSQ PVDF membrane (Sigma-Aldrich, ISEQ00010) at 400 mA for 75 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were visualized with either anti-rabbit IgG (whole molecule)-HPeroxidase (Sigma) or ECL Horseradish Peroxidase-linked anti-mouse (NA931 ...
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bioRxiv - Plant Biology 2019Quote: ... proteins were concentrated using Amicon Ultra concentrators (Millipore, MWCO 3,000 and 30,000). Proteins were analyzed for purity and structural integrity by SDS-PAGE and mass spectrometry.
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bioRxiv - Cell Biology 2019Quote: ... The target proteins were detected on the membranes by enhanced chemiluminescence (Millipore).
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bioRxiv - Synthetic Biology 2020Quote: ... The culture supernatant was loaded onto a protein A-sepharose column (Millipore), and the bound antibody was eluted by the addition of 0.2 M glycine–HCl (pH 2.7) ...
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bioRxiv - Biochemistry 2019Quote: ... gels were stained with InstantBlue™ Ultrafast Protein Stain (ISB1L, Sigma-Aldrich) according to the manufacturer’s instructions and the gels were imaged using LICOR Odyssey CLx ...
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bioRxiv - Biochemistry 2019Quote: ... gels were stained with InstantBlue™ Ultrafast Protein Stain (ISB1L, Sigma-Aldrich) according to the manufacturer’s instructions and the gels were imaged using LICOR Odyssey CLx ...
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bioRxiv - Cell Biology 2019Quote: ... The flow chamber was incubated with Protein G (Sigma, Cat. No. 08062) for 10 minutes followed by anti-his antibody (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Complexes were pulled down using Dynabeads Protein A/G (Sigma-Aldrich, 14321D) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were released by addition of 5 μg of RNase A (Sigma) in RNA digestion buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein was then transferred to a 0.45um Immobilon-P PVDF membrane (Millipore). After blocking for 1 hr in 5% nonfat dried milk in TBST ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein activity was measured by an ATPase activity kit (Sigma-Aldrich) as described by the manufacturer.