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Citations for Millipore Sigma :
2751 - 2800 of 10000+ citations for Mouse Anti Hepatitis C Virus NS3 Helicase Antibody 1847 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... SARS2-57 and SARS2-71 anti-S (VanBlargan et al., 2021) antibodies and HRP-conjugated goat anti-mouse IgG (Sigma, 12-349) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Cell Biology 2020Quote: ... the membrane was incubated with the primary antibody (rabbit anti-Sarm1, 1:500, ANASPEC 55381; Mouse anti-β-Tubulin, 1:2000, Sigma T5168) at 4°C overnight ...
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bioRxiv - Cell Biology 2019Quote: ... For primary antibodies we used hybridoma supernatant of monoclonal antibody 6E3 Rat anti-CLASP2 (Maffini et al., 2009) at 1:150 and Mouse anti-α-tubulin clone B-512 (Sigma Aldrich) at 1:10000 ...
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bioRxiv - Cancer Biology 2020Quote: ... transferred onto nitrocellulose membranes and incubated overnight at +4 with primary antibodies against PROX1 (goat-anti-human, AF2727, R&D, 1:1000) and vinculin (mouse anti-vinculin, V9131, Sigma, 1:1000). HRP conjugated secondary antibodies (Dako ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by 1 h incubation at RT in secondary antibodies (Anti-Rabbit: A0545; Anti-Mouse: A6782, Sigma-Aldrich Quimica S.A., Madrid, Spain) diluted 1:10,000 in blocking buffer ...
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bioRxiv - Cell Biology 2022Quote: ... the membranes were incubated with HRP-conjugated secondary antibody (1:10,000; anti-rabbit, Sigma-Aldrich A0545 or anti-mouse, Sigma-Aldrich A0168) in TBST with 1% milk ...
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bioRxiv - Cancer Biology 2022Quote: ... filters were incubated for 1 hour at RT with either goat-anti rabbit (1:5000) or goat-anti mouse (1:2000) horseradish peroxidase (HRP)-conjugated secondary antibodies (Merck Millipore, Darmstadt, Germany). Blots were developed using SuperSignal West Pico Chemiluminescent Substrate or Femto Maximum Sensitivity (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Systems Biology 2020Quote: ... Blots were incubated with appropriate secondary antibodies (Anti-Rabbit IgG-Peroxidase Sigma-Aldrich A0545 and Anti-Mouse IgG-Peroxidase Sigma-Aldrich A9044) and imaged using GE Amersham Imager 600.
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bioRxiv - Plant Biology 2021Quote: ... The glutathionylated VirE2 was detected by the anti-glutathione monoclonal primary antibody (My biosource, 1:1000) and anti-mouse (Sigma, 1:1000) secondary antibody (Klaus et al ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein detection was achieved using the following antibodies: anti-Toddler (rabbit, 1:500, provided by the Reversade Lab11) and anti-tubulin (mouse, 1:10000, Sigma-Aldrich T6074).
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bioRxiv - Neuroscience 2022Quote: ... Then membranes were incubated with anti-mouse or anti-rabbit horseradish peroxidase conjugated secondary antibodies (1:3000, Sigma Aldrich, St. Louis, MO) for 1 hr ...
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bioRxiv - Genetics 2024Quote: ... and secondary antibodies (anti-mouse HRP [1:2,000; Thermo Fisher Scientific] for TARS1 and anti-rabbit HRP [1:5,000; EMD Millipore] for actin) were applied in 2% milk solution ...
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bioRxiv - Microbiology 2023Quote: ... Horseradish peroxidase-conjugated secondary antibodies (anti-mouse or anti-rabbit, at dilution 1:10000) and Immobilon® Western Chemiluminescent HRP substrate (Merk Millipore, Sigma Aldrich) were used to visualize FLAG-FlaA/FlaB and untagged FlaA/FlaB.
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bioRxiv - Immunology 2023Quote: ... Sections were incubated for 48-72 hours with the following primary antibodies at 1:400: rabbit anti-Iba1 (Wako, 019-19741) and mouse anti-PSD-95 (Sigma-Aldrich, MAB1598). Sections were then washed 3x with PBS containing 0.05% Tween 20 and incubated with Alexa Fluorophore conjugated goat anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were incubated with secondary antibodies conjugated with PLA probes MINUS and PLUS: the PLA probe anti-mouse PLUS and anti-rabbit MINUS (Duolink®, Sigma-Aldrich). The incubation with all antibodies was accomplished in a humidified chamber for 1 h at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... Antibodies used in Western blot were (listed in Appendix Table S4): mouse monoclonal antibodies anti-β-actin and anti-UVRAG (both from Sigma-Aldrich), anti-LC3B (NanoTools) ...
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bioRxiv - Molecular Biology 2021Quote: Retroviral infection was conducted in 6-well plates using either 1 mL of unconcentrated virus or 200 μL of concentrated virus in 20 mM HEPES pH 7.4 and 8 μg/mL polybrene (Sigma-Aldrich). If selection was desired ...
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bioRxiv - Cancer Biology 2020Quote: ... Virus infection was carried out by incubating cells with cell medium containing indicated virus and 5 μg/ml polybrene (Sigma) for 8-12 hours ...
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bioRxiv - Neuroscience 2024Quote: ... Rat anti-PCDH10 antibody (Anti-OL-protocadherin antibody, clone 5G10, Sigma) or mouse anti-GAPDH (Merck ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa cells were seeded at a density of 2.5×106 cells/well in 12-well plates and spin-fected (2000 rpm for 2 hours at 37°C) with increasing concentrations of virus in the presence of 8µg/mL polybrene (Millipore TR-003-G lot#3287963). The next day ...
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bioRxiv - Microbiology 2023Quote: ... Boost vaccination was performed with the homologous virus inactivated at 4°C for 3 days with 0.1% beta propiolactone (BPL) (Sigma-Aldrich Corporation, St. Louis, MO) as previously described (66) ...
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bioRxiv - Cancer Biology 2023Quote: ... RMG-1 ARID1A-WT and ARID1A-KO cells were seeded at a density of 2.5×106 cells/well in 12-well plates and spin-fected (2000 rpm for 2 hours at 37°C) with increasing concentrations of virus in the presence of 8µg/mL polybrene (Millipore TR-003-G lot#3287963). The next day ...
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bioRxiv - Systems Biology 2021Quote: ... virus protein-bound host proteins were enriched by adding 50 µl anti-HA-agarose slurry (Sigma-Aldrich, A2095) with constant agitation for 3 hours at 4°C ...
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bioRxiv - Neuroscience 2020Quote: Virus was produced by polyethylenimine (PEI; Sigma-Aldrich) transfection of 89-90% confluent HEK293T cells with the viral vector together with the lentiviral packaging plasmids ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... or an empty vector control virus (Sigma SHC001V) followed by puromycin (1 μg/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... After virus concentration by PEG 8000 (Sigma, #P5413) and Benzonease treatment (Millipore ...
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bioRxiv - Immunology 2020Quote: ... TZM-bl cells (1 X 104) were added into the mixture virus-antibody mixture containing 25 μg/ml DEAE-dextran (Sigma). The plates were further incubated for 48 h and the extent of virus neutralization was assessed by measuring relative luminescence units in a luminometer (Victor X2 ...
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bioRxiv - Cell Biology 2024Quote: ... using as primary antibody mouse monoclonal antibody 6-11B-1 (Millipore Sigma) diluted 1:50 with PBS ...
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bioRxiv - Microbiology 2020Quote: ... Mouse monoclonal HA-Tag and Mouse Monoclonal Flag antibodies purchased from Sigma (USA); Goat anti-mouse IgG horseradish peroxidase-conjugated secondary antibody and goat anti-rabbit IgG horseradish peroxidase-conjugated secondary antibody purchased from Thermo Fisher Scientific (USA).
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bioRxiv - Cell Biology 2023Quote: ... Mouse Vinculin (V9131) and mouse Flag (F3165) antibody were purchased from Sigma-Aldrich. Antibodies against METTL14 (26158-1-AP) ...
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bioRxiv - Microbiology 2019Quote: ... antibody and rabbit anti-Flag antibody (Sigma) solution for 1 h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... the oocytes were blocked in washing buffer with 3% BSA for 1 h at room temperature and then incubated overnight at 4°C with FITC-labelled mouse anti-α-tubulin (1:200, Sigma-Aldrich) or mouse monoclonal anti-γ-H2AX antibody ...
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bioRxiv - Biophysics 2020Quote: ... nuclei were incubated with blocking solution (10% goat serum in PBS) for 30 min at 37°C and stained with mouse-anti-γH2AX (1/500, Millipore 05-636) for 1 hr at 37°C followed by O/N incubation at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated 18 hours at 4°C with mouse anti-TH (1/5000, MAB318, Sigma-Aldrich, Saint Louis, MO, USA) in blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated 18 hours at 4°C with mouse anti-TH (1/5000, MAB318, Sigma-Aldrich, Saint Louis, MO, USA) in blocking solution ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies were incubated overnight at 4°C at the following concentrations in IF Buffer: mouse anti-α-tubulin (DM1α, 1:1000, Sigma T6199), rabbit anti-rat kangaroo-Kid (3μg/ml ...
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The Hippo pathway terminal effector TAZ/WWTR1 mediates oxaliplatin sensitivity in colon cancer cellsbioRxiv - Cancer Biology 2023Quote: ... Immunoprecipitations were performed overnight at 4°C on a rocking wheel using mouse EZview Red anti-Flag M2 affinity gel (Sigma-Aldrich #F1804) after transfections of either p2xFlag CMV2 (empty vector) ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti-V5 and rabbit anti-ZBP1 (Sigma-Aldrich), with secondary HRP conjugated donkey anti-mouse or donkey anti-rabbit antibodies (Santa Cruz).
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-Anti-β-Tubulin (1:1000, Sigma, #T8328); rabbit anti-Tyrosine Hydroxylase (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... anti-mouse-STAR580 (Abberior) and anti-rabbit-Atto647 (Sigma) were diluted 1:100 ...
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Appropriate glycemic management protects the germline but not uterine environment in type 1 diabetesbioRxiv - Developmental Biology 2024Quote: ... and anti-MCT4 (donkey anti-mouse, 1:200, Sigma). Staining was performed overnight at 4 □C ...
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bioRxiv - Cancer Biology 2024Quote: ... and mouse anti-human anti-Vimentin (Sigma-Aldrich, CBL202) in 0.5% (v/v ...
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bioRxiv - Developmental Biology 2020Quote: ... Embryos were incubated in primary antibody overnight at 4° C (chick α-GFP, Abcam, Cat# AB13970, Lot# GF305729-1; mouse α- γ-tubulin, Sigma-Aldrich, Cat# T6557 ...
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bioRxiv - Pathology 2019Quote: ... rabbit anti-mouse von-Willebrand factor (vWF) and rabbit anti-mouse podocin (Sigma, St. Louis, MO). All secondary IgG class antibodies were from Thermofisher (Waltham ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse anti-FLAG M2 (F3165) and mouse anti-Acetylated Tubulin (T6793) were purchased from Sigma-Aldrich. The rabbit antibody specific for the DDDDK-tag (PM020 ...
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bioRxiv - Cell Biology 2019Quote: ... mouse cardiomyocytes were treated with 10 µg/ml Mitomycin C (Sigma Aldrich) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... at 56°C for 40min and re-probed with anti– beta-actin antibody for 1h (1:1000, Sigma, ref. A5441).
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bioRxiv - Immunology 2019Quote: ... Samples were then blocked in PBS with 0.5% Triton X-100 solution supplemented with 2% goat serum for 1 h at room temperature and incubated overnight at 4 °C with mixtures of the following primary antibodies: anti-centrin (clone 20H5; Millipore), anti-pericentrin (ab4448 ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were subsequently incubated in blocking buffer containing 0.1% Tween 20 and the appropriate primary antibodies for 1-2 hr at room temperature or 4°C overnight: rabbit anti-Myc (1:2,000, Sigma, C3956), mouse anti-FLAG (1:2,000 ...