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Citations for Millipore Sigma :
2701 - 2750 of 10000+ citations for Recombinant Human IL18 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... Protein concentration was measured using Bradford reagent (Sigma) and the entire L1 lysates were added to 25 ul magnetic Protein-G beads (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein concentration was measured using BSA (Sigma-Aldrich) serial dilutions and the “DCTM Protein Assay” kit (BioRad) ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were blotted onto polyvinylidene difluoride membranes (Millipore). The membranes were probed with the indicated antibodies (all from Cell Signaling) ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein was transferred to Immobilon-FL membrane (Millipore). To detect tubulin ...
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bioRxiv - Microbiology 2023Quote: ... 30 µl of agarose protein A beads (Millipore) washed with RNA extraction buffer were added to 1 mg of protein lysate for a 1.5 h incubation at 4 ℃ on a rocking platform ...
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bioRxiv - Physiology 2023Quote: ... proteins were transferred to polyvinyldienefluoride membranes (Millipore #IPFL00010) and subjected to western blotting as described previously5.
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bioRxiv - Microbiology 2023Quote: ... Protein concentration was determined by the Bradford (Sigma) method using 2 mg/mL BSA as a standard (ThermoFisher).
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bioRxiv - Molecular Biology 2023Quote: ... anti-Protein A antibodies (Sigma, P3775, 1:5000). IRDye secondary antibodies (800CW and 680RD ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were reduced using 1 mM DTT (Sigma) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: Active SLO protein was obtained commercially (Sigma Aldrich) or expressed and purified recombinantly in-house ...
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bioRxiv - Biophysics 2023Quote: ... Proteins were overexpressed in E.coli Rosetta (DE3) (Novagen) cells and cultured in LB broth to an OD600 of 0.6-0.8 ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were transferred onto Immobilon-P membranes (Millipore) and probed with anti-rabbit-peroxidase (Jacksons) ...
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bioRxiv - Cell Biology 2024Quote: ... and proteins alkylated with 55 mM iodoacetamide (Sigma) in 100 mM NH4HCO3 for 20 mins at RT (protected from light) ...
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bioRxiv - Cancer Biology 2024Quote: ... 50μg protein emulsified with complete Freund’s adjuvant (Sigma) was subcutaneously (s.c. ...
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bioRxiv - Neuroscience 2024Quote: Proteins were reduced (10 mM DTT, EMD Millipore) and alkylated (30 mM iodoacetamide ...
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bioRxiv - Biochemistry 2024Quote: ... and 0.1% protein inhibitor cocktail (Sigma- Aldrich: P8849)] ...
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bioRxiv - Neuroscience 2024Quote: ... using BCA Protein Assay (Sigma-Aldrich, CA, USA). For all calculations ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were wet transferred onto PVDF membrane (Millipore) for 1 h at 100 V ...
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bioRxiv - Neuroscience 2024Quote: Protein was extracted in RIPA buffer (R0278, Sigma) supplemented with protease inhibitor cocktail (P8340 ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were transferred onto polyvinylidene fluoride membrane (Millipore). Membranes were blocked with 10% non-fat milk in tris-buffered saline containing 0.3% Tween 20 (TBST ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were transferred to a PVDF membrane (Millipore). Membranes were blocked with 5% (wt/vol ...
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bioRxiv - Developmental Biology 2024Quote: ... and biotinylated-Hyaluronan Binding Protein (Millipore, 1:50) at 4°C ...
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bioRxiv - Systems Biology 2024Quote: ... The proteins were reduced with TCEP (Sigma-Aldrich), final concentration 5 mM for 30 min at 37 °C and alkylated with 10 mM IAA (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... Parallel protein A/G beads (EMD Millipore, #IP0515ML), H3K4Me (Cell Signalling Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were then transferred to nitrocellulose (Sigma #10600001) using a wet transfer apparatus (Bio-rad #1704070) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 1% protein inhibitor cocktail (100×) (Sigma-Aldrich).
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was quantified using BCA1 from Sigma. Thirty micrograms of protein lysate were separated by 6% Bis-Tris Gel ...
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bioRxiv - Bioengineering 2021Quote: ... Marimastat ((2S,3R)-N-[(1S)-2,2-dimethyl-1-(methylcarbamoyl)propyl]-N’,2-dihydroxy-3-(2-methylpropyl)butanediamide), ɑ2-antiplasmin (SRP6313-100) and recombinant plasminogen activator inhibitor 1 (PAI-1, 528208) were purchased from Sigma-Aldrich (Saint-Louis, MI). Sodium alginate (SA ...
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bioRxiv - Microbiology 2022Quote: ... Expression of recombinant antigens in induced and un-induced controls was assessed by immunoblot using a monoclonal anti-poly- histidine antibody (Millipore-Sigma, diluted 1:2000) after SDS-PAGE ...
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bioRxiv - Developmental Biology 2024Quote: ... EBs and CBs were cultured in the presence of either 3mg/l recombinant SPARC (S5174-25UG, Sigma-Aldrich; 942-SP-050, R&D Systems), various amounts of anti-SPARC antibodies (Santa Cruz ...
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bioRxiv - Genomics 2024Quote: ... The reaction products were analyzed on 8% PAGE-SDS gels with silver staining or Western blotting for PAR using a recombinant anti-poly-ADP-ribose binding reagent (Millipore, Cat# MABE1031; RRID: AB_2665467).
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bioRxiv - Cancer Biology 2024Quote: ... 100 µM recombinant calmodulin (Enzo, cat. BML-SE325-0001) and 2 mM Adenosine 5’-triphosphate (ATP) disodium salt hydrate (Sigma-Aldrich, cat. A1852-1VL) for 30 min at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... 21 and 42 with 50 μg of each of five recombinant LMWs in alum mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, #70323-44-3). ELISA was performed to measure serum responses to antigen (see methods below ...
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bioRxiv - Immunology 2021Quote: ... Liver triglyceride and total cholesterol concentrations were measured using commercial kits (all from Instruchemie) and expressed as nanomoles per milligram of total protein content using the Bradford protein assay kit (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2021Quote: CDK5 was immunoprecipitated from cells samples at different circadian times (CTs) (circa 200 μg of protein extracts. After immunoprecipitation at 4°C overnight with 2x Protein A agarose (Sigma-Aldrich), samples were diluted in washing buffer and split in two halves ...
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bioRxiv - Microbiology 2021Quote: ... moscoviensis was assessed by measuring total cell protein with the enhanced test tube protocol of the BCA protein assay (Sigma-Aldrich).
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bioRxiv - Bioengineering 2021Quote: Protein adsorption to the films was analyzed by incubation in a bovine serum albumin-FITC protein solution (1mg/ml, Sigma Aldrich) overnight at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... pLysS cells expressing His-topoIB and T18 tagged genome segregation proteins in different combinations using the kit Protein G immunoprecipitation from Sigma-Aldrich as described previously (Maurya et ...
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bioRxiv - Microbiology 2021Quote: ... were immunized in a prime-boost-boost regimen with post-fusion hMPV B2 F protein (50 µg protein/mouse) in a water in oil emulsion with Titermax Gold adjuvant (Sigma-Aldrich) via subcutaneous (SC ...
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bioRxiv - Cell Biology 2021Quote: ... Then cell lysates were subject to protein immunoprecipitations using indicated beads (For N-Flag-S-protein-tagged, FLAG M2 (Sigma-Aldrich) or S-protein beads (Millipore ...
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bioRxiv - Bioengineering 2022Quote: ... Protein content was measured by the BSA (cells) or microBSA (EVs) protein assay according to the manufacturer’s instructions (Sigma Aldrich, USA).
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bioRxiv - Microbiology 2020Quote: Protein concentration was determined by the bicinchoninic acid protein assay kit (BCA-Pierce, USA) using bovine serum albumin (BSA, Sigma-Aldrich) as standard.
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bioRxiv - Systems Biology 2021Quote: ... the gel was scanned for total protein quantification and the proteins were subsequently transferred to an Immobilon-FL PVDF membrane (CAT # IPF00010, EMD Millipore). The membrane was probed with Rabbit anti-RAN (CAT # PA 1-5783 ...
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bioRxiv - Immunology 2021Quote: ... The protein concentration in the final samples was measured with the use of Bicinchoninic Acid Kit for Protein Determination (Sigma-Aldrich) and a 96-well plate analyzer (Tecan Infinite 200 PRO).
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: Native PAGE-separated proteins or SDS PAGE-separated proteins were then transferred to PVDF membranes (EMD Millipore, catalog #IPVH00010, lot #R1EB02212) and subjected to Western blotting analysis as described previously (Li et al ...
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bioRxiv - Plant Biology 2023Quote: ... then proteins were extracted by adding 5 volumes of protein extraction buffer containing 1X MS-SAFE protease and phosphatase inhibitor (Sigma MSSAFE) and 1mM PMSF ...
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bioRxiv - Neuroscience 2023Quote: ... The ATP level for each sample was normalized to the protein content of each sample using the BCA protein assay kit (Sigma-Aldrich). Results are expressed as fold change ± S.E.M ...
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bioRxiv - Microbiology 2024Quote: ... followed by protein G coupled magnetic beads at room temperature for 10 minutes (PureProteome Protein G Magnetic Bead System, Millipore LSKMAGG). The beads were removed using a magnetic rack ...
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bioRxiv - Microbiology 2024Quote: ... The obtained protein lysates were pre-cleared by centrifugation and incubated with 100 μl (50% slurry) of protein A beads (Millipore Sigma), coated specific antibodies or corresponding species-specific IgG isotype control in 1 ml of 1 × IP buffer ...
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bioRxiv - Molecular Biology 2024Quote: The proximity ligation assay (PLA) was conducted to determine potential protein–protein interactions using the Duolink in situ detection kit (Sigma-Aldrich) in accordance with the manufacturer’s instructions ...