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Citations for Millipore Sigma :
2701 - 2750 of 10000+ citations for 7 Methylimidazo 1 2 a pyridine 3 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... follicular fluid (FF) was slowly layered on 3:1 Ficoll gradient (Histopaque 1077, Sigma-Aldrich) and centrifuged at 400 g for 20 min ...
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bioRxiv - Cell Biology 2021Quote: ... with a 3:1 concentrated solution of 20 nm:5 nm colloidal gold (Sigma Aldrich) blocked with bovine serum albumin ...
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bioRxiv - Bioengineering 2022Quote: ... 1 μM α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA, No.A6816, Sigma-Aldrich) is added in ACSF to enhance the Ca2+ signals ...
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bioRxiv - Cell Biology 2021Quote: ... then 85°C for 10 minutes and transfected in a 3:1 polyethylenimine (PEI, Sigma):DNA ratio into 70% confluent HEK 293A cells (ThermoFisher ...
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bioRxiv - Biochemistry 2020Quote: N-protein was dyed by adding (3:1) Atto 488 NHS ester (Millipore Sigma 41698) to purified protein and incubating mix at 30°C for 30 minutes ...
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bioRxiv - Microbiology 2019Quote: ... Cells were washed twice with PBS and anti-Gal-3 antibody (1:50; Sigma-Aldrich) was added ...
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bioRxiv - Molecular Biology 2019Quote: ... Resumption of meiosis was prevented with 0.2 mM 3-isobutyl-1-methyl-xanthine (IBMX, Sigma). MII oocytes were obtained from animals superovulated with 7 IU of PMSG administered on day 1 and 7 IU of hCG administered 46 – 48 h post PMSG ...
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bioRxiv - Biochemistry 2021Quote: ... The 3’ biotinylated TAR and RNA 30-mer (Table 1) were chemically synthesized (Sigma Aldrich). RNA (50 pmol ...
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bioRxiv - Neuroscience 2022Quote: ... the 3:1-DMEM/F12-medium was supplemented with 10 μM retinoic acid (Sigma, #R2625), and the medium was daily exchanged ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 3-isobutyl-1-methyl xanthine and poly-lysine were from Sigma Aldrich (St Louis, MO). All other reagents were of analytical grade.
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bioRxiv - Cell Biology 2022Quote: ... Sections were incubated with 3% hydrogen peroxidase (Sigma-Aldrich, St. Louis, MO, H1009, 1:10) for 20 min and incubated with a blocking reagent (Vector Laboratories ...
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bioRxiv - Biochemistry 2024Quote: ... The membranes were blocked for 1 hour in blocking buffer [3% Skim milk (Sigma-Aldrich) in T-TBS buffer (0.05% Tween 20 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated in rabbit anti-GluR2/3 (AB1506, Millipore Sigma, Burlington, MA, 1:200) and mouse anti-calretinin (MAB1568 ...
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bioRxiv - Cell Biology 2023Quote: ... 1 cm3 tumors were resected and embedded in 3% low gelling agarose (Sigma-Aldrich #A701) prepared in PBS ...
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bioRxiv - Biophysics 2023Quote: ... 1.5 equivalents of diethylaminosulfur trifluoride (3 ml of 1 M solution in dichloromethane, Sigma-Aldrich) were added dropwise under argon ...
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bioRxiv - Cancer Biology 2023Quote: ... for 1 min and blocking was performed by 3% bovine serum albumin (#A7906, Sigma-Aldrich) for 10 min at RT ...
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Juxtacrine DLL4-NOTCH1 signaling between astrocytes drives neuroinflammation via the IL-6-STAT3 axisbioRxiv - Neuroscience 2023Quote: ... Anti-RNA binding fox-1 homolog 3 (NEUN) (rabbit) was from Millipore (Billerica, MA, USA). Anti-human-DLL4 (Rabbit ...
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bioRxiv - Molecular Biology 2024Quote: ... in a ratio of 1:3 (μg DNA/μg PEI) in DMEM (Sigma-Aldrich, D6429) without supplements ...
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bioRxiv - Cancer Biology 2024Quote: ... (2) 2% Alconox (Sigma 242985 ...
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bioRxiv - Cancer Biology 2023Quote: ... (2) 2% Alconox (Sigma, 242985 ...
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bioRxiv - Cell Biology 2020Quote: Cultured CT26 cells were collected and their viability was assayed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma, St. Louis, MO, USA) colorimetric method ...
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bioRxiv - Neuroscience 2019Quote: ... BW723C86 (α-methyl-5-(2-thienylmethoxy)-1H-Indole-3-ethanamine monohydrochloride) and a primary antibody raised against β-actin were purchased from Sigma (USA). Other primary antibodies ...
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bioRxiv - Biophysics 2021Quote: ... Membranes were washed with PBST 2-3 times and bands were visualized upon addition of SigmaFast BCIP®/NBT tablets (Sigma B5655) (Fig ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were restreaked onto SD/-Trp/-Leu and incubated at 30 °C for 2–3 days before performing a colony-lift β-galactosidase assay (X-gal, Sigma-Aldrich) according to the Yeast Protocols Handbook (PT3024-1 ...
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bioRxiv - Cell Biology 2021Quote: ... Oocytes were surgically removed from anesthetized frogs (40, 46) under anesthesia (2 g/L Tricain, 3-aminobenzoic acid ethyl ester, Sigma A-5040). Preparation of defolliculated oocytes was carried out as described in (106) ...
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bioRxiv - Biophysics 2020Quote: ... oocytes were incubated twice (1st 45 min, 2nd variable) in OR-2 containing 3 mg/ml collagenase type IV (Sigma, C-9891). Defolliculated oocytes were stored in ND96 solution containing (in mM) ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.2 mg/mL sheared salmon sperm DNA and 2 mg/mL BSA) containing 50 ng of or HLA-B-biotin DNA probe (5’ TGTCCTAGCAGTTGTGGTCATCGGAGCTGTGGTCGCTGCTGTGAT-biotin 3’) (Sigma-Aldrich, USA) in a humidified chamber overnight ...
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bioRxiv - Cancer Biology 2022Quote: Cell proliferation was assessed by the colorimetric assay of sulforhodamine B 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate (SRB) (Sigma Aldrich, USA)[25] ...
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bioRxiv - Biochemistry 2019Quote: ... All other chemicals including palmitate and 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) (MTT) were obtained from Sigma-Aldrich (St. Louis, USA).
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bioRxiv - Developmental Biology 2021Quote: Larvae at 2-6 dpf were first anesthetized with 0.03 % Ethyl 3-aminobenzoate methane sulfonate salt (Sigma-Aldrich, St. Louis, MO, USA) and pinned onto a Sylgard-filled recording chamber (I-2450 ...
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bioRxiv - Cancer Biology 2021Quote: ... Viable cells were determined post treatment using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich, USA; Cat# M2128) assay by measurement of absorbance at 570 nm.17 The relative rate of cell growth for each cell line was factored into the analysis by subtracting the absorbance at time 0 from both the control and treatment groups ...
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bioRxiv - Immunology 2022Quote: ... type 2 and type 3 were mixed and subsequently concentrated using 10 kDa Amicon® Ultra Centrifugal Filters (Merck Millipore, Billerica, MA) to a nominal concentration of 10000-16000-32000 DU/mL.
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bioRxiv - Plant Biology 2022Quote: Kinase activity assay was carried out by mixing 2 μg of CORK1KD or GST-CORK1KD-G748E with 3 μg of myelin basic protein (MBP; Sigma-Aldrich, Germany) in kinase reaction buffer (25 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2024Quote: ... The number of viable cells was determined by mitochondrial conversion of 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma Aldrich, Missouri, USA) to formazan ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then cleared by replacing ethanol with ethyl cinnamate (ECi; Ethyl 3-phenyl-2-propenoate; Sigma-Aldrich, order no. 112372-100G). After 30-60 min ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were washed 3 times with 1x PBS and incubated for 2 hours with 5% goat serum (NGS -Sigma-Aldrich, G9023) in 1x PBS with 0.3% Triton (Sigma-Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... The surface of the coverslips was functionalized by amine using the silanization solution of N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (Sigma-Aldrich, 8191720100), acetic acid ...
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bioRxiv - Cell Biology 2024Quote: ... logarithmically growing vps4Δ CDC48 or cdc48-3 cells were shifted to 37 °C for 2 h and simultaneously treated with 50 µM MG-132 (Sigma, Cat. # 474787) or the respective vehicle ...
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bioRxiv - Biophysics 2023Quote: ... the sample was resuspended in 100 μL 1 x DPBS and incubated for 1 hour in the dark with ∼ 3 μL of the MPM-2 antibody (anti-phospho-Ser/Thr-Pro, Cy5 conjugate) (Millipore, Sigma-Aldrich, USA) that stains cells containing mitosis-specific phophorylations ...
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bioRxiv - Molecular Biology 2023Quote: ... The locus with expected insertion was amplified by PCR using 5 μL KOD Hot Start 2× Master Mix (Sigma Aldrich 71842-3), 0.2 μL each primer at 10 μM ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Cellular dehydrogenase activity was analyzed by the conversion of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma Aldrich, St. Louis, MO) as described.[22e ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were washed 2× in PBS and then counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; 1:10 000; Sigma) for 10 min at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were permeabilized with 0.5 % TritonX-100 (Wako) in 1x PBS for 1-2 h and blocked in blocking solution (2 % BSA (Sigma-Aldrich), 1 % DMSO ...
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bioRxiv - Cell Biology 2021Quote: ... diluted in 1% normal donkey serum in PBS with 2 µg/mL 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... All strains were cultured in either rich (YPD: 1% yeast extract, 2% peptone, and 2% dextrose) or complete synthetic minimal (CSM) media (Sigma) lacking appropriate amino acids with 2% dextrose ...
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bioRxiv - Molecular Biology 2021Quote: ... The ionic liquid solution was dialyzed into lysis buffer (6 M urea, 2 M thiourea, 2% CHAPS, 1% DTT) using a 3k MWCO cellulose membrane (Amicon Ultra, Millipore).
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bioRxiv - Bioengineering 2022Quote: ... ALP activity in cell lysates was determined by adding 20 μl of 0.75 M 2-amino-2-methyl-1-propanol (A65182, Sigma-Aldrich) to 80 μl sample in 96-wells assay plates ...
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bioRxiv - Immunology 2022Quote: ... ALP activity was determined by adding 20 μl of 0.75 M 2-amino-2-methyl-1-propanol (A65182, Sigma-Aldrich) to 80 μl sample in 96-wells assay plates ...
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bioRxiv - Microbiology 2019Quote: ... referred to here as hCMEC were obtained from Millipore and were maintained in an EndoGRO-MV complete medium kit supplemented with 1 ng/ml fibroblast growth factor-2 (FGF-2; Millipore) (81–84) ...
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bioRxiv - Immunology 2020Quote: ... the membranes were incubated with the appropriate horseradish-peroxidase conjugated secondary Ab (1:5000 in TBST + 2% BSA; 2 h at room temperature; anti-mouse A4416 and anti-rabbit A0545, Sigma). The immunoreactivity was detected by ECL reagents (Amersham) ...