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Citations for Millipore Sigma :
2701 - 2750 of 10000+ citations for 6 7 Dichloro 1H indole 2 3 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Confluent ESCs monolayers were decidualized in DMEM/F12 containing 2 % FBS supplemented with 0.3 mM N6,2′-O-Dibutyryladenosine 3′,5′-cyclic monophosphate sodium salt (cAMP) (Sigma-Aldrich, USA), 10 nM β-Estradiol (E2 ...
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bioRxiv - Immunology 2021Quote: ... were pelleted at 3.000g for 3 minutes and induced by resuspension in YNB-CAA + 2% D-(+)-Galactose (Sigma G0625-500G) + phosphate buffer + P/S induction medium at a final OD600 of 0.5 ...
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bioRxiv - Bioengineering 2022Quote: ... the cells were kept at dim light (2-3 μE) for 16 h and plated on fresh TAP plates containing 10 μg/mL Paramomycin (Sigma). Plates were incubated for 10 d at 30-50 μE constant light ...
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bioRxiv - Bioengineering 2022Quote: ... with a 3:1 M ratio of 5-norbornene-2-carboxylic acid (mixture of endo and exo isomers; Millipore-Sigma) to HA-TBA repeat units ...
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bioRxiv - Bioengineering 2022Quote: ... the wells were rinsed 3 times with PBS and covered with 500 μL 2% Alizarin Red solution (Sigma-Aldrich, 05600) diluted in UPW for 15 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... containing 0.1 % Tween-20 and 2 mM EDTA before incubating in a blocking solution (3 % acetylated bovine serum albumin (Sigma, MO), 0.1 % Tween-20 ...
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bioRxiv - Microbiology 2022Quote: ... Liquid cultures were grown in a mixture of 2:3 ratio of Todd-Hewitt media with 0.5% yeast extract (Sigma-Aldrich), and Brain-Heart Infusion media (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2022Quote: ... media was exchanged for 2 mL of culture media supplemented with 500 µM 3-indoleacetic acid (IAA, Millipore Sigma, I2886). Each dish was transfected with 400 ng of the reporter plasmid using X-tremeGENE HP DNA transfection reagent (Roche ...
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bioRxiv - Molecular Biology 2023Quote: Cytotoxicity of Quercetin and other natural compounds was evaluated using MTT (3-(4, 5- dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye) assay (Sigma Aldrich). Following the drug treatment in triplicate at indicated concentrations ...
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bioRxiv - Neuroscience 2023Quote: ... Proliferative capability of BMECs was tested by 3-[4,5-dimethylthiazyol-2-yl] -2,5-diphenyltetrazolium bromide (MTT, 5mg/ml, Sigma, cat# CT01) assay ...
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bioRxiv - Cell Biology 2022Quote: ... cells were synchronised in early S phase by two consecutive rounds of treatment with 2 mM thymidine and 3 ug/ml (Sigma) aphidicolin ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the cells were incubated for 30 minutes with 3 µmol/L 4,5-diaminofluorescein diacetate (DAF-2 DA; Sigma-Aldrich, D225). After that ...
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bioRxiv - Systems Biology 2024Quote: ... 2M thiourea, 100mM Tris-HCl, pH 8, 150mM NaCl, 1mM EDTA, phosphatase inhibitor cocktail 2 and 3, 10mM NaF; Sigma), reduced with 10mM DTT (dithiothreitol ...
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bioRxiv - Neuroscience 2024Quote: ... and 2-(14-carboxytetradecyl)-2-ethyl-4,4-di methyl-3-oxazolidinyloxy (16-doxyl-stearicacid, 16-DSA, cat. no. 253596) from Sigma-Aldrich. 5-DSA (spin probe containing the nitroxide group attached on C5 that is located on the opposite terminal relative to the charged carboxyl fatty acid terminus ...
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bioRxiv - Neuroscience 2024Quote: ... Flies were housed singly for 2–3 days after the procedure and were subsequently head-fixed to a custom mount with eicosane (Sigma) and imaged on a Movable Objective Microscope with resonant scanners (MOM ...
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bioRxiv - Genetics 2024Quote: ... differentiation of iPSCs was induced through the Wnt modulation method with 7 μM glycogen synthase kinase 3 b (GSK3b) inhibitor CHIR99021 (Selleckchem) and 5 μM inhibitor of WNT production 2 (IWP2) (Sigma). Basal Media was composed of RPMI media supplemented with B27 (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... tricaine-anesthetized 3 or 4 days-post fertilization (dpf) larvae were mounted in 2% low-melting point agarose (Sigma-Aldrich) on a 35 mm glass bottom dish (#1.5H Glass ...
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bioRxiv - Genetics 2023Quote: ... animals of similar age were depleted of AID::GFP expression by culturing for 2 h on NGM plates containing 100 µM auxin (3-indoleacetic acid; Sigma). Directly after depletion ...
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bioRxiv - Plant Biology 2023Quote: ... and incubated at 28°C shaking at 200 rpm for 2–3 h before plating on LB agar supplemented with 50 µg/ml Kanamycin (Merck Millipore). The plates were incubated for 2– 3 days at 28°C ...
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bioRxiv - Cell Biology 2023Quote: ... Penis and lymph nodes were dissected and fixed for 1.5-2 h in 3% paraformaldehyde (penis) or overnight in 1% paraformaldehyde (PFA, Sigma, P6148) at 4°C (lymph nodes ...
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bioRxiv - Immunology 2023Quote: ... for 1-2 hours at RT followed by 3 washes in PBS + 0.1% Tween 20 (PBS-T; Sigma P7949-100ML). Primary antibody was then fixed to the tissue for 30 minutes in 4% PFA for 30 minutes at RT followed by 3 washes with PBS-T ...
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bioRxiv - Bioengineering 2023Quote: ... 3 µL of 2 M CaCl2 and 5 µL of 10 mg/mL ε-aminocaproic acid (ε-ACA) (Sigma-Aldrich), and 1 µL of 1U/µL Thrombin (MP Biomedicals ...
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bioRxiv - Cell Biology 2023Quote: ... composed of Egg yolk L-α-Phosphatidylcholine (EyPC, Avanti Polar), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS, Avanti Polar) and Cholesterol (Sigma) (50:20:30 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were inoculated in YEP containing 3% lactic acid and treated with a final concentration of 2% galactose (Sigma-Aldrich). For the inducible degradation of the proteins ...
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bioRxiv - Neuroscience 2023Quote: ... Then the brains were dissected to 2 mm slices and incubated for 10 minutes in freshly prepared 0.5% TTC solution (2, 3, 5-Triphenyltetrazolium chloride, Sigma-Aldrich). Afterward ...
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bioRxiv - Cancer Biology 2023Quote: ... two to four-month-old mice were gavaged 3 times over 5 days with 2 mg tamoxifen (TX; Sigma #T5648) in corn oil (Sigma #C8267) ...
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bioRxiv - Neuroscience 2023Quote: ... 1 mm3 tissue pieces from 2-3 brains were pooled and enzymatically digested in a dissociation medium containing 0.16 mg/mL cysteine (Sigma C9768), 7 U/mL Papain (Sigma P3125) ...
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bioRxiv - Developmental Biology 2023Quote: ... lentiviruses were generated by transfecting 3 x 106 HEK-293T cells in antibiotic-free medium with 6 µg of total DNA in a 1:2:3 ratio of pMD2.G:psPAX2:transfer plasmid using polyethylenimine (PEI) (Sigma-Aldrich). 48 h after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... (phenylmethylsulfonyl fluoride, PMSF 1mM; 1-chloro-3-tosylamido-4-phenyl-2-butanone, TPCK, 10 μg/ml; aprotinin, 10 μg/ml Sigma) incubated for 30min on ice ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions (some of which were pooled from tumors harvested from 2-3 mice) were stimulated for 5 h with PMA (5 ng/ml, Sigma) and ionomycin (500 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... The activity of trypsin was inhibited by dropping the pH to 2-3 by the addition of 10% trifluoroacetic acid (TFA, Sigma). The samples were loaded on Evosep tips to separate the digested peptides using nanoflow reversed-phase chromatography with an Evosep One liquid chromatography (LC ...
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bioRxiv - Microbiology 2024Quote: ... Each extract was resuspended in 170 µL of 100% methanol containing isotopically labeled internal standards (5-50 µM of 13C,15N Cell Free Amino Acid Mixture, #767964, Sigma; 1 ug/mL 2-amino-3-bromo-5-methylbenzoic acid, ABMBA, #R435902, Sigma) and centrifuge-filtered (0.22 µm hydrophilic PVDF membrane ...
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bioRxiv - Bioengineering 2024Quote: ... and polyIC incubation was evaluated using the MTT (3-(4, 5dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye) assay (Sigma Aldrich). The cells were seeded into 96 well-format cell culture plates at a seeding density of 8ξ103 cells per well ...
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bioRxiv - Plant Biology 2024Quote: Arabidopsis Ws-2 CCR2::LUC WT seeds were surface sterilized and plated on 1x MS medium containing 3% w/v sucrose (Sigma), 1.5% w/v phytoagar (Duchefa) ...
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bioRxiv - Molecular Biology 2021Quote: ... heterozygote (HNF4α fl/+, Cre/+), and knockout (HNF4α fl/fl, Cre/+) mice (N=3-6 per group) were administered tamoxifen (T5648, Sigma, 5 mg/kg ip in corn oil) once daily for 2 d ...
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bioRxiv - Neuroscience 2021Quote: ... The left MFB was injected with either 1μL of 6-Hydroxydopamine hydrochloride (6-OHDA, (Sigma-Aldrich) 3.2µg/µL free-base in a 0.02% ascorbic acid solution (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: Serum IL-6 levels were quantified using a commercial mouse IL-6 ELISA kit (RAB0308, Sigma) according to the manufacturer’s instructions and analyzed using spectra-iMAX (Molecular Devices).
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bioRxiv - Cell Biology 2021Quote: ... 6 mM ATP or 6 mM ADP (conjugated with sodium salt; Sigma-Aldrich, St. Louis MO) were included along with the contents described above ...
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bioRxiv - Physiology 2019Quote: ... 6 mM D-glucose (Sigma-Aldrich), and 34 mM NaCl (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... 100 nM 6-Benzylaminopurine (BAP, Sigma) and 100 nM Zeatin (Sigma) ...
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bioRxiv - Genetics 2019Quote: ... 10−6 M β-estradiol (Sigma) and 20 ng/ml stem cell factor ...
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bioRxiv - Cancer Biology 2020Quote: ... afatinib (850140-72-6, Sigma Aldrich), gefitinib (S1025 ...
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bioRxiv - Bioengineering 2020Quote: ... Aminocaproic acid (6-aminohexanoic acid, Sigma) at a final concentration of 2 mg/mL was added to both media ...
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bioRxiv - Neuroscience 2020Quote: ... 6 μg/mL polybrene (Sigma-Aldrich) and 10 mM HEPES (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... the coumarin 6 (Sigma-Aldrich 546283) was dissolved in methanol (VWR BDH2029 ...
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bioRxiv - Neuroscience 2020Quote: ... the fluorescein (Sigma-Aldrich F245-6) was dissolved in NaOH 1.0N in aqueous solution (VWR BDH7222) ...
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bioRxiv - Neuroscience 2022Quote: 6-OHDA-HCl (Sigma-Aldrich, #H4381) was dissolved in a saline solution (NaCl 0.9% w/v ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Ferric chloride (4-6%; Sigma, UK) was applied to the adventitial surface of the vessel for 3 mins ...
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bioRxiv - Cell Biology 2022Quote: ... 10 mM MgCl2 · 6 H2O (Sigma), 0.1% w/v Triton X-100 (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... 6 mM MgCl2 (Sigma, catalog #: M0250), 1 mM CaCl2 (Sigma ...