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Citations for Millipore Sigma :
2651 - 2700 of 10000+ citations for Anti FLAG M5 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... The supernatant was mixed with ANTI-FLAG M2 affinity beads (Sigma-Aldrich) and incubated on a rocker for 2 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies used Drosophila tissues include mouse anti-Flag (Sigma, 1:200) and rabbit anti-HA (CST ...
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bioRxiv - Cell Biology 2024Quote: ... rabbit anti-FLAG (Sigma, Cat no. F7425, Lot #06M4757, IF 1:100).
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies were used: mouse anti-Flag F7425 (Sigma-Aldrich, F7425); rabbit anti-Phospho-PAK4/5/6 (pSer474 ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant containing Abo1 was incubated with anti-FLAG M2 resin (Sigma) at 4 ℃ for 2 h with rotating ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were detected using anti-FLAG (Sigma-Aldrich, ST. Louis, MO, USA) and HRP-labeled anti-mouse IgG (Cytiva ...
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bioRxiv - Genomics 2024Quote: ... and 10ul of Anti-FLAG M2 Magnetic Beads (Sigma-Aldrich, Cat. #M8823) was used for each reaction in pull-down ...
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bioRxiv - Cell Biology 2024Quote: ... The primary antibodies were a mouse anti-FLAG antibody (F1804, Sigma-Aldrich), a rat anti-HA antibody (11867423001 ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were incubated with anti-FLAG M2 affinity beads (Sigma-Aldrich) for 1 hour at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... at 4°C before either antibody (mouse anti-FLAG, Sigma-Aldrich F1804) or glutathione sepharose was added for overnight incubation ...
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bioRxiv - Molecular Biology 2024Quote: ... and the supernatant was incubated with Anti-Flag M2 affinity gel (Sigma). The affinity gel was washed twice with lysis buffer and twice with SEC buffer (50 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... rabbit anti-V5 (PRB-189P, Covance, 1/1000) or mouse anti-FLAG (M2, Sigma, 1/1000) antibodies ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 µg of the respective antibodies (Anti-ERα; ThermoFisher MA5-13065 or Anti-Flag; Sigma F3165) were added to the lysate and incubated on a rotating wheel at 4°C overnight.
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bioRxiv - Microbiology 2021Quote: ... Anti-Flag (catalog number F3165) and Anti-FASN (catalog number SAB4300700) antibodies were purchased from Sigma and used at a final concentration of 1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... The lysates were incubated with anti-HA or anti-Flag Agarose beads (Sigma, A2095, and A9469) as indicated overnight at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was incubated with 25 μL of anti-FLAG M2 or anti-HA resin (Sigma) for 2.5 hours ...
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bioRxiv - Microbiology 2020Quote: ... and anti-HA- and anti-FLAG-conjugated agarose beads were purchased from Sigma (St. Louis, MO). The following siRNAs targeting human genes were obtained ...
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bioRxiv - Genetics 2020Quote: ... Epitope-tagged proteins were immunoprecipitated with either anti-Flag- or anti-HA-coated magnetic beads (Sigma and Pierce ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse anti-FLAG M2 (F3165) and mouse anti-Acetylated Tubulin (T6793) were purchased from Sigma-Aldrich. The rabbit antibody specific for the DDDDK-tag (PM020 ...
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bioRxiv - Molecular Biology 2020Quote: Anti-Flag M2 agarose beads (A2220) and anti-HA agarose beads (A2095) were purchased from Sigma. Anti-Chk1 was purchased from Santa Cruz (sc-56288) ...
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bioRxiv - Molecular Biology 2019Quote: ... 300 μl of CHAMP buffer containing 4 nM Alexa488-conjugated anti-Flag antibody (Alexa Fluor 488 antibody labeling kit, Thermo Fisher; monoclonal BioM2, Sigma-Aldrich) was injected to wash off unbound RNP and label DNA-bound RNP complex ...
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bioRxiv - Biochemistry 2023Quote: ... 1:2500, anti-Ubiquitin P4D1, Biolegend, 1:10,000, anti-UBR4/p600 ab86738, Abcam, 1:5000, anti-FLAG M2, Sigma F1804 ...
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bioRxiv - Neuroscience 2020Quote: ... and FLAG sequence (FLAG, mouse, 1:200; Sigma; RRID: AB_259529). As secondary antibodies we used Alexa Fluor 488–coupled donkey antibodies recognizing chicken IgG (1:800 ...
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bioRxiv - Cell Biology 2019Quote: ... Flag-ERK2 was immunoprecipitated using Flag M2-conjugated agarose (Sigma) and washed at least three times with salt-adjusted Buffer A prior to denaturation by boiling at 100°C in Laemmli sample buffer.
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bioRxiv - Microbiology 2024Quote: ... a commercial primary FLAG antibody (α-FLAG, Millipore cat# F1804), or a commercial primary His antibody (α-His ...
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bioRxiv - Microbiology 2021Quote: ... and PilA2-Flag was detected using a horse radish peroxidase (HRP)-coupled primary anti-Flag antibody at a 1:2000 dilution (Sigma, cat. no. SAB4200119). Enhanced chemiluminescence (ECL ...
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bioRxiv - Biochemistry 2020Quote: ... RPS2-Flag tagged 40S ribosomes were immuno-precipitated by addition of 50 µL washed anti-Flag M2 affinity agarose gel (Sigma Aldrich, A2220-5mL) and 5 µL RNasin Plus (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... The overexpressed 3X FLAG-tagged cyclin-Cdk1 complexes were then purified by immunoaffinity chromatography using ANTI-FLAG M2 affinity agarose beads (Sigma-Aldrich Cat #A2220) and eluted with 0.2 mg/mL 3X FLAG peptide (Sigma-Aldrich Cat #F4799) ...
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bioRxiv - Biochemistry 2022Quote: ... Western blots were carried out with two different antibody setups: (i) FLAG tag was incubated with monoclonal ANTI-FLAG® M2-Peroxidase (HRP) produced in mouse (Sigma-Aldrich #A8592) diluted 1:3 000 ...
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bioRxiv - Molecular Biology 2023Quote: ... The Cas9 protein contained an N-terminal FLAG-tag to confirm expression (mouse anti-3x FLAG primary antibody, 1:250 dilution, RT, 3 h, Sigma-Aldrich Cat# F1804). Cells were washed with PBS buffer ...
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bioRxiv - Cell Biology 2023Quote: ... the Anti-Flag M2 Affinity Gel was incubated with 2x vol of 100 µg/ml Flag-Peptide (in TBS, Sigma-Aldrich, cat.no. F3290) at 4 °C and 350 rpm agitation for 30 min ...
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bioRxiv - Cell Biology 2022Quote: Co-immunoprecipitation was performed using the FLAG immunoprecipitation kit (Millipore Sigma, #FLAGIPT1-1KT) following manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... lysates were processed for co-IP analysis by the FLAG Immunoprecipitation Kit (Sigma), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... M2-FLAG (Sigma) at 1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... Flag (F1804, Sigma), HA (H6908 ...
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bioRxiv - Cell Biology 2020Quote: ... FLAG (Sigma-Aldrich), hexa-histidine tag (Qiagen) ...
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bioRxiv - Molecular Biology 2021Quote: ... Flag (Sigma # F3165). The secondary antibodies used were ...
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bioRxiv - Molecular Biology 2021Quote: ... FLAG (F3165; Sigma), GFP (9996 ...
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bioRxiv - Neuroscience 2021Quote: ... Flag (Sigma, F1804). Following primary antibody incubation ...
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bioRxiv - Molecular Biology 2021Quote: ... FLAG (Sigma - F3165), H2AQ105me from (Tessarz et al ...
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bioRxiv - Cancer Biology 2019Quote: ... FLAG (Sigma, F1804). Densitometry was conducted using ImageJ software ...
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bioRxiv - Molecular Biology 2019Quote: ... FLAG (A8592, Sigma), HIV1 p24 (ab9071 Abcam) ...
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bioRxiv - Cell Biology 2019Quote: ... FLAG peptide (Sigma) was applied to the column to elute the FLAG protein complex according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... FLAG (Sigma; F1804), GRK1 (abcam ...
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bioRxiv - Molecular Biology 2019Quote: ... α-FLAG (Sigma, F3161), α-H3K9me2 (Abcam ...
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bioRxiv - Microbiology 2019Quote: ... α-Flag-M2 (Sigma), or α-CyaA[3D1] (#EG800 Kerafast)] were used 1:1000 in blocking buffer and detected with HRP-conjugated secondary antibodies (1:5000 ...
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bioRxiv - Cell Biology 2019Quote: ... Flag (Sigma, F3165); MG-132 (Selleck) ...
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bioRxiv - Cancer Biology 2020Quote: ... FLAG (F3165, Sigma), α-Tubulin (ab7291 ...
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bioRxiv - Cancer Biology 2021Quote: ... FLAG (Sigma#F1804), Actin (CST#3700 ...
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bioRxiv - Biochemistry 2020Quote: ... FLAG (F7425, Sigma), H3 pS10 (9701 ...