Labshake search
Citations for Millipore Sigma :
2601 - 2650 of 3774 citations for FGF 21 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... then 50 μL of alkaline phosphatase-conjugated secondary goat anti-Human anti-IgG (Sigma Cat # A9544), anti-IgA (Abcam Cat # AB97212) ...
-
bioRxiv - Bioengineering 2021Quote: Immortalized human neural progenitor cells derived from the ventral midbrain (ReNCell VM) were obtained from Millipore. Cells were expanded on laminin-coated tissue culture flasks ...
-
bioRxiv - Neuroscience 2020Quote: ... Cultured human embryonic kidney 293 (HEK293) cells were transfected using 2 μg/μl polyethylenimine (Sigma-Aldrich) with indicated plasmids for the expression of either Flag-KCTDs alone or in combination with Flag-Cav2.3 ...
-
bioRxiv - Cell Biology 2021Quote: ... Cytokines: interferon gamma (IFNγ) (SRP3058) and human Interleukin-4 (IL-4) (I4269) were from Sigma-Aldrich, UK ...
-
bioRxiv - Bioengineering 2021Quote: Human serum albumin (A1653) and mouse serum albumin (A3139) were purchased from Sigma (St Louis, MO). Fluorescently labeled human serum albumin (HS1-S5-1 ...
-
bioRxiv - Neuroscience 2020Quote: ... were co-transfected into U87 human primary glioblastma cells by using ESCORT V transfection reagent (Sigma). The fold increase in relative luciferase activity is a product of the luciferase activity induced by TCF7l2-S2 or TCF7l2-E2 (backbone vector pCMV ...
-
bioRxiv - Cancer Biology 2019Quote: ... cells were incubated with FITC-conjugated goat anti-human IgG (Fc specific) (Sigma St-Louis, MI) or FITC-conjugated goat anti-mouse IgG (H+L ...
-
bioRxiv - Biochemistry 2021Quote: ... membranes were washed and incubated with HRP-conjugated anti-human IgG antibody (Sigma-Aldrich; 1:10.000) followed by chemiluminescence detection.
-
bioRxiv - Cell Biology 2021Quote: ... HAP1 cells were transfected with 600 ng/ml human VRK2 MISSION® esiRNA (EHU070731, Sigma-Aldrich) using Lipofectamine RNAiMAX (13778075 ...
-
bioRxiv - Cell Biology 2021Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1: 85580C, Sigma-Aldrich, MO, USA), and 20 ng/mL human bFGF] for 35 days ...
-
bioRxiv - Cell Biology 2021Quote: ... Fibronectin-coated coverslips were prepared by first covering them with 100 µL of human (Sigma F0556) or bovine (Sigma F01141 ...
-
bioRxiv - Microbiology 2020Quote: ... Parasites were cultured at 4% haematocrit (using human O+ RBCs) and synchronised with 5% sorbitol (Sigma). For transfection ...
-
bioRxiv - Systems Biology 2021Quote: Dispersed human islets and iPSC-beta cells were exposed to 0.5 mmol/L palmitate (Sigma-Aldrich) for 24 h and 48h ...
-
bioRxiv - Neuroscience 2021Quote: ... This was followed by blocking for 1 hour in 1% human serum albumin (HSA; Sigma-Aldrich) and 0.03-0.1% Triton X-100 dissolved in TBS and 200 ug/ml Digitonin (D141-100MG ...
-
bioRxiv - Microbiology 2021Quote: ... ChIP MIC in serum was determined in the presence of 50% (heat inactivated) human serum (Sigma) and 50% MHB-II ...
-
bioRxiv - Microbiology 2019Quote: ... The immortalized human cerebral microvascular endothelial cell line hCMEC/D3 (59) was purchased from Millipore Sigma and maintained in Endothelial Cell Growth Basal Medium-2 (EBM2 ...
-
bioRxiv - Cell Biology 2020Quote: ... DNA constructs corresponding to the mature form of human UBTD1 were subcloned from pEGFP-N1 (Novagen) to a retroviral vector compatible plasmid (PPRIG ...
-
bioRxiv - Cell Biology 2021Quote: ... FN coating was accomplished by adding phosphate buffered saline (PBS, Cellgro) and human plasma fibronectin (Millipore) to six-well plates (Corning) ...
-
bioRxiv - Biochemistry 2022Quote: ... Type O+) at 4% haematocrit in NaHCO3 and AlbumaxII/human serum supplemented RPMI (RPMI (Sigma Aldrich), 25 mM HEPES (GIBCO) ...
-
bioRxiv - Bioengineering 2022Quote: ... Scaffolds were washed with PBS and incubated with or without 100 nM human insulin (Sigma-Aldrich) in 100 μL glucose-free DMEM/F12 for 1 hour at 37 °C ...
-
bioRxiv - Neuroscience 2022Quote: 90,000 human NPCs were plated in 12-well plates coated with Poly-L-Ornithine (Sigma Aldrich) and Laminin (Sigma Aldrich ...
-
bioRxiv - Pathology 2022Quote: ... and fibrin deposits using DyLight488-anti-human/mouse fibrin antibody (8 μg, clone 59D8, Sigma-Aldrich). All antibodies and dyes were injected intravenously 15 min prior to intravital imaging ...
-
bioRxiv - Genomics 2022Quote: ... full length human LINC00881 was cloned into p3XFLAG-CMV-7 (Millipore Sigma, St. Louis, MO, USA) vector and amplified in DH5α strain of Escherichia coli (E coli ...
-
bioRxiv - Bioengineering 2022Quote: ... The antibodies used were a) purified CHO h-IgG b) IgG from human serum (Sigma-Aldrich, resuspended in deionised H2O and stored at 4°C) ...
-
bioRxiv - Cell Biology 2022Quote: ... 0.324 and 0.108ng of a reference DNA (Human genomic DNA from buffy coat, Sigma cat# 11691112001) are included in each PCR run so that the quantity of targeted templates in each research sample can be determined relative to the reference DNA sample by the standard curve method ...
-
bioRxiv - Cancer Biology 2022Quote: ... mouse Tollip (TRCN0000066136 and TRCN0000066137) and human RIOK1 (TRCN0000295923 and TRCN0000037399) were obtained from Millipore Sigma.
-
bioRxiv - Immunology 2020Quote: ... permeabilized RBCs were stained with anti-human spectrin antibody (1 in 50 dilution; S3396, Sigma Aldrich) concurrently with GNA lectin (8 μg/ml ...
-
bioRxiv - Microbiology 2020Quote: ... were treated with or without 25-100 U/mL of human recombinant IFN-α (Sigma-Aldrich) for 4-12 hours after which total RNA was isolated and subjected to qRT-PCR in order to measure expression of ISGs.
-
bioRxiv - Molecular Biology 2021Quote: ... followed 47-48 hours later with 5 IU of human chorionic gonadotrophin (Sigma-Aldrich, CG10-1VL) before being bred ...
-
bioRxiv - Immunology 2020Quote: ... supplemented with 10% heat-inactivated human AB plasma from healthy donors (SIGMA, male AB, H-4522), 1% l-glutamine ...
-
bioRxiv - Immunology 2020Quote: ... supplemented with 10% heat-inactivated human AB plasma from healthy donors (Sigma, male AB, H-4522), 1% l-glutamine ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: HEK293T cells (Human embryonic kidney, Lehner laboratory stocks) were cultured in Iscove’s Modified Dulbecco’s Media (Sigma) supplemented with 1% GlutaMAX ...
-
bioRxiv - Immunology 2020Quote: ... RPMI1640 + 2.05 mM L-Glutamine (Fisher) was supplemented with 10% heat inactivated human AB serum (Sigma), 12.5 mM HEPES (Fisher) ...
-
bioRxiv - Immunology 2020Quote: ... Cells obtained from tumor and ascites were cryopreserved in 50% heat inactivated human AB serum (Sigma), 40% RPMI-1640 (Fisher ...
-
bioRxiv - Microbiology 2021Quote: The human embryonic kidney 293T cells (HEK293T) were maintained in Iscove’s modified Dulbecco’s medium (IMDM; Sigma), human THP-1 cells were maintained in Roswell Park Memorial Institute medium (RPMI ...
-
bioRxiv - Biochemistry 2021Quote: ... cDNAs for human OCT4 and SOX2 were cloned into the pFLAG-CMV5a mammalian expression vector (Sigma).
-
bioRxiv - Bioengineering 2020Quote: ... To the solution was added pre-warmed human cathepsin B (20 ng µL−1, EMD Millipore) in 30 µL MES buffer ...
-
bioRxiv - Cell Biology 2022Quote: ... The cells were washed with PBS and media replaced with human adipocyte differentiation media (Sigma Aldrich). The medium was replaced with fresh human adipocyte differentiation media every 3 days as per the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2019Quote: ... EVs were incubated with Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) at different final concentration (0 to 10 µg/ml) ...
-
bioRxiv - Neuroscience 2019Quote: ... and PAK/cofilin mechanism experiments used human embryonic kidney cells (HEK293) cultured in DMEM:F12 (Sigma, D6421) supplemented with 10% foetal bovine serum (ClonTech ...
-
bioRxiv - Physiology 2019Quote: ... mice were fasted for 5 h before human insulin (0.75 units/kg body weight, Sigma Aldrich) was injected intraperitoneally ...
-
bioRxiv - Microbiology 2019Quote: ... Human neutrophils were separated from peripheral blood by density gradient centrifugation using Histopaque (Sigma–Aldrich, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... Monoclonal anti-β-actin (mouse anti-human primary, CAT# A2228) antibody was purchased from Sigma-Aldrich. Proteome Profiler Human Apoptosis Array kit (CAT# ARY009 ...
-
bioRxiv - Physiology 2021Quote: ... Insulin concentrations after the 16 h hyperinsulinemia treatment were determined using human insulin RIA kit (Millipore). To mimic starvation ...
-
bioRxiv - Genetics 2020Quote: ... Caveolin knockdown was achieved by transfection with siRNA against caveolin (MISSION® esiRNA human CAV1, Sigma) or negative control siRNA (MISSION® siRNA Universal Negative Control #1 ...
-
bioRxiv - Microbiology 2020Quote: ... membrane-extracted proteins were incubated for 1.5 hours at 4°C with purified human IgG (Sigma) that had been previously coupled to CNBr-activated Sepharose beads (GE Healthcare) ...
-
bioRxiv - Microbiology 2021Quote: For expression of C13/N15 labeled recombinant human wildtype-MIF BL21-(DE3)-Rosetta-pLysS bacteria (Novagen) were transformed with pET11a-MIF and spread on LB-Agar plates containing 50 µg/mL carbenicillin and 34 µg/mL chloramphenicol ...
-
bioRxiv - Synthetic Biology 2020Quote: ... the blots were incubated with 1:20000 HRP-conjugated rabbit anti-human IgG (Sigma-Aldrich, #A8792). For SARS-CoV-2 antigen detection the blots were incubated with 1:2000 Anti-His mouse monoclonal primary antibody (Qiagen ...
-
bioRxiv - Molecular Biology 2021Quote: ... 10 µg/ml BSA fraction V and 10 µg/ml human recombinant in sulin (both Sigma)) ...
-
bioRxiv - Biochemistry 2020Quote: ... The shRNA lentiviral plasmids (pLKO.1-puro) for human/mouse/rat frataxin were purchased from Sigma. The RefSeq used was NM-008044 ...