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Citations for Millipore Sigma :
2601 - 2650 of 10000+ citations for 6 Chloro N methyl pyrimidine 4 5 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... for 1 h at room temperature simultaneously with 4′,6-diamidino-2-phenylindole (DAPI: 1:2500, D9542; Sigma-Aldrich-Aldrich, USA) and Phalloidin Alexa488 (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were mounted on glass microscopy slides with DAPI-containing mounting medium (4’,6-Diamidino-2-phenylindole dihydrochloride, Vectashield-H-1200-10, Sigma-Aldrich). All brain slices were imaged by a confocal microscope (LSM 710 ...
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bioRxiv - Cell Biology 2023Quote: ... Cell nuclei were stained with DAPI (4 ’,6-diamidino-2-fenilindol) and slides were mounted using FluorSave™ Reagent (Merck Millipore). The sections were observed and visualized on a Leica DM2500 fluorescent microscope (Leica Microsystems) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 1 min with 4’,6-diamidino-2-phenylindole (DAPI; 0.5 μg/ml, Sigma-Aldrich, cat# D8417) diluted in PBS to label cell nuclei ...
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bioRxiv - Developmental Biology 2023Quote: ... 25 mg of minced tissue was digested on ice for 4 minutes in 1 ml of Collagenase/Elastase/Dispase enzyme mix (6 mg/ml Collagenase A (Sigma, 10103578001), 4.3 units/ml Elastase (Worthington ...
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bioRxiv - Cancer Biology 2024Quote: ... HUVECs (4.5·106 cells/mL) and MSCs (6·105 cells/mL) were suspended in EGM-2MV supplemented with thrombin (4 U/mL) (Sigma-Aldrich). A solution of fibrinogen (8 mg/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of 0.4–0.5 μg of plasmid and dsRNAs were transfected into BmN4 cells (4–6 × 104 cells per glass bottom 35 mm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche), and the cells were fixed 5–6 days later ...
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bioRxiv - Cell Biology 2024Quote: Cells were cultured in 6-well plates with EGM-2 medium supplemented with 4% dextran (Sigma-Aldrich, St. Louis, MO, 31392). Hemodynamic flows were applied to using a 1o tapered stainless steel motorised cone (UMD-17 (Arcus20 Technology ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were rinsed in PBS followed by a nuclear staining with DAPI (4’,6-diamidino-2-fenylindool, 1:1000 in PBS, 32670, Sigma-Aldrich). All sections were mounted with Mowiol and a glass cover slide ...
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bioRxiv - Neuroscience 2024Quote: Mouse vibrissae were carefully isolated from the upper lip pad of 4-week-old C57BL/6 male mice and cultured in Williams E medium (Sigma-Aldrich), supplemented with 1% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... Cells fixed in 4% PFA were blocked in PBS containing 5% donkey serum (Sigma-Aldrich #D9663) and 0.3% Triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Immunology 2019Quote: ... 4°C for 5 min and washed once with a PBS containing 1% BSA (Sigma Aldrich). The single cell suspensions used for 10X Genomics scRNAseq analysis were cleared of red blood cells by using a Red blood cell lysis buffer (eBioscience ...
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bioRxiv - Synthetic Biology 2021Quote: ... 0.5 μL of matrix solution (5 mg/mL α-cyano-4-hydroxycinnamic acid from Sigma-Aldrich dissolved in 50% acetonitrile containing 0.1% trifluoroacetic acid ...
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bioRxiv - Microbiology 2020Quote: ... Parasites were cultured at 4% haematocrit (using human O+ RBCs) and synchronised with 5% sorbitol (Sigma). For transfection ...
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bioRxiv - Microbiology 2020Quote: ... left 5 min at RT for cell attachment and fixed with 4% paraformaldehyde (PFA) (Sigma P6148) for 10 min at RT ...
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bioRxiv - Cell Biology 2020Quote: Cells were incubated at 4°C for 5 min with mouse serum-opsonized zymosan (Sigma, Z4250) or L ...
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bioRxiv - Bioengineering 2020Quote: ... washed thrice (5 min) with PBS and fixed in 4% w/v paraformaldehyde (PFA, Sigma-Aldrich) for 15 min at room temperature (RT) ...
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bioRxiv - Cell Biology 2020Quote: ... male Lgr5DTRGFP mice aged 4-5 months were administered 50 μg/kg diphtheria toxin (322326 Sigma) or saline vehicle intraperitoneally ...
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bioRxiv - Bioengineering 2021Quote: ... was dissolved in a mixture of 5:4 (mL:mL) DI water:DMSO (Sigma-Aldrich, Saint Louis, MO) at 40 °C ...
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bioRxiv - Cell Biology 2019Quote: ... the coverslips were pre-coated overnight at 4°C with 5 µg/ml fibronectin (EMD Millipore) in 1× PBS and washed with 1× PBS immediately before added the cell mixtures ...
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bioRxiv - Genetics 2019Quote: BzATP (2′(3′)-O-(4-Benzoylbenzoyl) adenosine 5′-triphosphate triethylammonium salt) was purchased from Millipore Sigma and ...
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bioRxiv - Genomics 2021Quote: ... Blots were then exposed for 4-5 days to a GE Storage Phosphor screen (Millipore Sigma) before imaging on a Typhoon™ FLA 7000 imager (GE) ...
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bioRxiv - Neuroscience 2022Quote: ... 4-5-week old mice were treated with 8 mg tamoxifen (TAM, T5648, Sigma-Aldrich, USA) solved in 200 ul corn oil (C8267 ...
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bioRxiv - Immunology 2022Quote: ... for 4 hours in the presence of 5 μg/ml Brefeldin A (Sigma-Aldrich, München. Germany), followed by treatment with 2 % paraformaldehyde and 0.1 % IGEPAL®CA-630 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... 4 °C) and resuspended in 5 mL of Phosphate Buffered Saline (PBS, pH 7.2, Sigma-Aldrich). The resulting pellet was resuspended in 1/50 of the initial volume of the initial sample ...
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bioRxiv - Cell Biology 2024Quote: MEF WT cells were treated with either 4 mM valproic acid (VPA, 1069-66-5, Sigma) or 1µM 3-deazaneplanocin (DZNep ...
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bioRxiv - Genetics 2023Quote: Approximately 5 mm-thick cross-sections of liver tissue were fixed in 4% paraformaldehyde (Sigma-Aldrich) at room temperature for 4 hours or at 4°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... then 5 uL of 10 mg/mL of proteinase K (Millipore Sigma, Cat. No. 70663-4) was added ...
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bioRxiv - Cell Biology 2023Quote: ... zymosan (life technologies, added at a 1:5 ratio) or R848 (Sigma-Aldrich; 4 μg/ml). After this time ...
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bioRxiv - Molecular Biology 2021Quote: ... SC-ura / SC-ura + 50µg/ml 6-azauracil (6-AU, Sigma), SC-leu / SC-leu + 0.4mM CuSO4 (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... 6-hydoxydopamine (6-OHDA; cat. #H4381; Sigma-Aldrich, St. Louis, MO) was dissolved in vehicle solution (0.1 % ascorbic acid [cat ...
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bioRxiv - Physiology 2021Quote: ... and IL-6 (KIT ELISA IL-6-RAB0308-1KT, Sigma-Aldrich) in BALF following the kit instructions ...
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bioRxiv - Microbiology 2022Quote: ... The forward primer contained a 6-FAM (6-carboxyfluorescein) tag (Sigma). EMSA reaction mixtures (25 μL volume ...
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bioRxiv - Neuroscience 2024Quote: For the intranasal administration of 6-hydroxydopamine (6-OHDA; Sigma-Aldrich), MMTV-PyMT +/-female mice at 1 week after the tumor onset were briefly anesthetized with 3% isoflurane ...
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bioRxiv - Cell Biology 2024Quote: ... cells were selected with 6 µg/mL 6-thioguanine (Sigma-Aldrich). The generated mutant cells were stocked in CELLBANKER 1 (Nippon Zenyaku Kogyo).
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bioRxiv - Biophysics 2023Quote: ... Both resiniferatoxin (Alomone Labs) and 6’-iodoresiniferatoxin (6’-iRTX, Sigma-Aldrich) were dissolved in ethanol to create a 1 mM stock and subsequently diluted to a 200 nM working concentration with the bath solution ...
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bioRxiv - Plant Biology 2019Quote: ... and axr3-3 were expressed as 6X His-tag (N-terminal) fusion proteins in Escherichia coli strain Rosetta™ DE3 competent cells (Supplementary Table 4; Novagen, product code: 70954). These proteins were expressed in minimal media with 13C D-glucose and 15N ammonium chloride ...
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bioRxiv - Cell Biology 2021Quote: ... The activity of β-hexosaminidase and glucosylceramidase β measured by a fluorometric method using 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide (Sigma, Cat#69585) and 4-Methylumbelliferyl β-D-glucopyranoside (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... All standard amino acids were purchased from AAPPTec except for: N-α-Fmoc-O-t-butyl-L-trans-4-hydroxyproline (Hyp) was purchased from EMD Millipore (Billerica, MA), Fmoc-γ-carboxy-glutamic acid γ,γ-di-t-butyl ester (Gla ...
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bioRxiv - Microbiology 2019Quote: ... A 200 μL aliquot of a solution containing 100 μg/μL of XTT (2, 3-(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide) (Sigma-Aldrich, St Louis, MO, USA) and 10 μg/μL of PMS (phenazine methosulfate ...
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bioRxiv - Evolutionary Biology 2021Quote: ... extracts were redissolved in 20 µl of n-hexane containing 1 µg/ml n-nonane (Sigma-Aldrich) as an internal standard and vortexed at maximum speed for 10 seconds ...
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bioRxiv - Biophysics 2022Quote: ... encoding CPB was cloned as N-terminal His6 or N-terminal GST fusion into pET19-b (Novagen) using NcoI and BamHI sites ...
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bioRxiv - Bioengineering 2019Quote: ... followed by addition of 1.12 g N-hydroxysuccinimide [9.72 mM] and 1.79 g of N-(3-(dimethylamino)propyl)-N’-ethylcarbodiimide hydrochloride [9.36 mM] (Sigma). The resulting solution was mixed at room temperature (RT °C ...
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bioRxiv - Cell Biology 2021Quote: ... The dye solution is prepared dissolving the dye powder in anhydrous N,N-dimethylformamide (DMF, Sigma-Aldrich). The dye to protein molar ratio was adjusted between 2 - 5 in order to provide a DOL around 1 ...
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bioRxiv - Microbiology 2020Quote: ... gondii were mutagenized with ENU (N-Ethyl-N-nitrosourea, N3385-1G, Sigma Aldrich; 100-200 μg/ml) in DMEM without FBS for 2 hours at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Evans blue dye was extracted from the organs by the addition of N,N-dimethylformamide (Sigma-Aldrich) (adjusted to 4 mL/g wet tissue ...
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bioRxiv - Microbiology 2020Quote: ... We then prepared a solution of 457 mM N,N-Bis(carboxymethyl)-L-Lysine-hydrate (Sigma-Aldrich) in 1 M NaHCO3 (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by addition of 100 µl N-tert-Butyldimethylsilyl-N-methyltrifluoroacetamide + 1% tert-Butyldimethylchlorosilane (Sigma Aldrich, 00942) for 1 hour at 60°C ...
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bioRxiv - Developmental Biology 2020Quote: ... and N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT D5942, 1μM, Sigma-Aldrich).
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bioRxiv - Neuroscience 2022Quote: ... The interior walls of the capillaries were silanized with silan vapors (N,N-Dimethyltrimethylsilylamine, Sigma Aldrich, France) for 15 min and dried at 200°C for 100 min ...