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Citations for Millipore Sigma :
2551 - 2600 of 10000+ citations for Urotensin 2 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... the cells were incubated with the corresponding primary antibodies diluted in blocking solution: anti-tyrosine hydroxylase (TH) (Millipore, #MAB318; 1:2000) or anti-NeuN (Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were blocked with 1 drop of blocking solution provided with the Duolink® PLA Probes (DUO92002 and DUO92004, Sigma-Aldrich) for 1h at 37°C in a humid chamber ...
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bioRxiv - Developmental Biology 2023Quote: ... all samples were incubated with Blocking One Histo (Nacalai Tesque) for 10 min at RT and 10% normal donkey serum (Sigma-Aldrich) in PBS/0.5% Triton X-100 with 5% Blocking One Histo for 30 min at RT.
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bioRxiv - Biochemistry 2024Quote: ... Cells were incubated with the following primary antibodies diluted in blocking buffer overnight at 4°C: mouse anti-FLAG M2 (1:500; Sigma; F1804), rabbit anti-PEX14 (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... preparations were washed for 1 hour in blocking buffer containing 5% (vol/vol) normal goat serum (NGS) (Cat# G9023, Sigma-Aldrich) in PBT then incubated for 24–48 hr at 4 °C in the primary antibody mouse anti-GFP (1:500 in blocking buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... After blocking in LiCOR blocking buffer the following primary antibodies were used: DDX5 (Pab204, gift from Prof. Frances Fuller-Pace; clone204, Sigma-Aldrich), DHX9 (ab26271 ...
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Molecular identification of ALDH1A1 and SIRT2 in the astrocytic putrescine-to-GABA metabolic pathwaybioRxiv - Neuroscience 2023Quote: ... The cells were then incubated with primary antibodies in blocking solution in the following composition: guinea-pig anti-GABA antibody (1:1000, AB175, Millipore, USA), chicken anti-GFAP antibody (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... Embryos were washed multiple times with PBST (0.8% Triton-X 100 in PBS) and incubated in blocking buffer (10% NGS (Sigma-Aldrich, #G6767) and 4% BSA (Sigma Aldrich ...
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bioRxiv - Physiology 2023Quote: ... and then incubated overnight at 4°C in primary antibodies diluted in 50% blocking buffer / 50% Tris buffered saline (bioWORLD #42020056–3) + 0.1% TWEEN (Sigma-Aldrich #P9416) (TBS-T) ...
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bioRxiv - Systems Biology 2023Quote: ... Thereafter the blocking buffer was discarded and the membranes were incubated with 1:1000 dilutions of mouse anti-FLAG M2 antibody (Sigma-Aldrich) in 1X TBST (overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... They were then incubated in primary antibodies (supplementary table 7) diluted in Odyssey blocking buffer supplemented with 0.2% (v/v) Tween 20 (Sigma-Aldrich, P9416) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... GFP-Trap®_MA beads were washed with low-salt buffer and blocked for 60 mins at room temperature in Casein Blocking Buffer (Sigma-Aldrich) supplemented with 50 μg/mL Heparin ...
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bioRxiv - Biophysics 2023Quote: ... we added a 1:400 solution of primary antibody in blocking buffer (anti-GM130 ab52649 Abcam, anti-tubulin Millipore Sigma T6199). We incubated coverslips with the primary antibody overnight at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... We blocked the membrane for 1 hour with blocking buffer: 5% bovine serum albumin (BSA, BioShop) in tris-buffered saline (BioShop) with Tween 20 (Millipore Sigma) (TBST ...
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bioRxiv - Microbiology 2023Quote: ... in PBS 1X at RT for 40 min and incubated with a blocking buffer containing 3% bovine serum albumin (BSA, Sigma Aldrich) in PBS 1X RT for 40 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies were diluted in blocking reagent to detect Pax7 (Developmental Studies Hybridoma Bank, 1:10) and laminin (Sigma, 1:50). After 16h incubation at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were subsequently blocked for 1 hour at room temperature in blocking buffer (PBS with 5% skimmed milk powder (Sigma-Aldrich) and 0.1% Tween) ...
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bioRxiv - Cell Biology 2023Quote: ... Specimens were washed with phosphate-buffered saline and blocking solution (1% BSA, 0.1% gelatin from cold-water fish skin (Sigma-Aldrich G7041), 0.5% Triton X-100 and 0.05% Tween-20 (Sigma-Aldrich P1379 ...
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bioRxiv - Cancer Biology 2023Quote: ... cleared biopsies were blocked for 3–4 h at room temperature (RT) or overnight at 4°C in blocking buffer (PBS (Sigma-Aldrich), 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were kept in the blocking solution (5 % Normal Goat Serum, 0.1 % Triton-X, 0,1 % DMSO in PBS) (Sigma Life Science, 1002135493) for at least 1 hour before incubating in the primary AB containing-blocking solution overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed once with PBS for 10 min followed by a blocking- permeabilization step for 1 hr at RT in blocking buffer (PBS/ 5% donkey serum Bio-Rad C06SB / 0.1% Triton Sigma X100). Primary antibodies diluted in blocking buffer were then added to the cells (Table 1 ...
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bioRxiv - Developmental Biology 2023Quote: ... the slides were rinsed three times with PBS and blocked for 1 hour at RT in blocking buffer made with 3% BSA (Merck Millipore, 0218072801) and 0.02% Triton X-100 (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... for 30 minutes at room temperature and then overnight at 4°C in blocking solution containing mouse anti-Alpha-tubulin (1:500, Sigma, #T9026). Next ...
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bioRxiv - Neuroscience 2023Quote: ... PLUS and MINUS PLA probes were diluted in the Duolink Antibody diluent in 1:5 ratio with blocking buffer from the PLA kit (Millipore Sigma). The coverslips were then incubated in PLA probe solution for 1 hour at 37°C in humidity chamber ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos after in situ HCR were washed with PBST and soaked in blocking solution [5% heat inactivated (56°C, 30 min) sheep serum (S2263, Sigma-Aldrich) in PBST] at room temperature for 2 hours ...
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bioRxiv - Bioengineering 2023Quote: ... followed by 1-hour blocking of non-specific binding sites with PBS with 5%v/v bovine serum albumin (BSA) (Sigma Aldrich) at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... membranes were incubated ON at 4 °C with primary antibodies diluted in blocking solution supplemented in 0.05 % Tween-20 (Sigma-Aldrich Corp.); anti-HSP70 (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... Gradual conversion of each sample to 1XPBT was performed using a diluted series of SSC/PBT and samples were blocked in blocking buffer for 3 hours at room temperature before adding 1:10000 of anti-DIG antibody (Millipore/Sigma) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Once dried, coverslips were placed upside down on a drop of hybridizing solution (70% formamide, 0.5% blocking reagent (Sigma, Cat:11096176001), 10 mM Tris-HCl pH 7.2 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with homemade anti-RBM20 (1:400) primary antibody in blocking buffer [PBS containing 5% goat serum (cat# G6767; Sigma-Aldrich), 0.1% Triton X-100 (cat# X100 ...
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bioRxiv - Cell Biology 2022Quote: ... and then incubated with primary antibodies in blocking buffer [1:400 homemade rabbit anti-RBM20,15 or 1:500 anti-α-actinin (cat# A7811; Sigma-Aldrich)] overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... and then incubation overnight at 4°C in blocking solution with primary antibodies: mouse anti-CRE (Sigma, F3165-2MG, 1:1000); mouse anti-Luciferase (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... After washing 3x in ddH20 slides were blocked for 1 hour with blocking solution: 5% normal donkey serum (Sigma, Cat#D9663), 0.1% Tween-20 in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... followed by permeabilization and blocking for 1 hour at room temperature in PBS with 0.5% Triton-X-100 (Sigma #X100-500ML)/0.2% Tween-20 (Sigma #P1379)/2% BSA (Sigma #A7906) ...
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bioRxiv - Immunology 2023Quote: ... Blocking was performed in 5% non-fat dry milk in PBS containing 0.2% Tween 20 (Sigma-Aldrich, St. Louis, Missouri, USA). Membranes were incubated in primary antibodies for 1-3 h at room temperature (20-25°C ...
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bioRxiv - Immunology 2023Quote: ... in PBS for at least 30 min at 37 °C and permeabilized with PBS containing 0.1 % Triton-X for 5 minutes and blocked with blocking solution (3 % BSA in PBS) at RT for 30 minutes (all reagents from Sigma-Aldrich). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells where thrice washed in PBS then incubated in blocking buffer (5% Donkey Serum (Jackson Immuno Research Labs 017-000-121, 1% BSA (Sigma A9647), 0.1% Triton X-100 (Sigma T8787 ...
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bioRxiv - Neuroscience 2023Quote: ... Free-floating brain sections were then incubated for 1 hour at 4C in a blocking solution of phosphate-buffered saline (PBS) containing 3% bovine serum albumin (BSA, Sigma; A4503) (vol/vol ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with anti-calbindin-D28k mouse monoclonal antibody (1:2000 dilution by the blocking solution, C9848, Millipore Sigma) at 4 °C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... brain sections were washed three times for 15 min with 1X TBS and then incubated for 1.5 h in blocking solution: 2.5% Donkey Serum (Sigma, Merck, Germany, S30); 2.5% Goat Serum (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were then incubated overnight at 4°C with the primary antibody diluted in blocking solution (5% goat and/or donkey serum in PBS, #G9023 and #D9663 Sigma-Aldrich). Cells were washed with complete PBS 3 times for 5min at RT and then incubated with the following secondary antibodies for 1h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Free-floating brain sections were then incubated for 1 hour at 4C in a blocking solution of phosphate-buffered saline (PBS) containing 3% bovine serum albumin (BSA, Sigma; A4503) (vol/vol ...
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bioRxiv - Physiology 2023Quote: ... the secondary antibody was applied by incubating the slides for 1 hour at room temperature with blocking buffer containing Alexa Fluor 647-conjugated goat anti-mouse antibody (1:1000, Sigma, SAB4600354). The slides were then briefly rinsed in PBS-Tw before a 10-minute incubation in PBS-Tw containing 1:2000 SYTOX Orange (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... Coverslips were washed twice more with 0.01M PBS before blocking in 5% (v/v) normal donkey serum (NDS; catalogue # S30-100ml, Sigma Aldrich, UK) in 0.01M PBS (3% Tween-20 ...
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bioRxiv - Genetics 2023Quote: ... The next morning membranes were washed once with blocking buffer for 5-10min and incubated with horseradish peroxidase-conjugated secondary antibodies (Sigma-Aldrich) diluted 1:10 000 in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Overnight primary antibody incubation was performed on a rocker in blocking solution containing the Anti-Tyrosine Hydroxylase (1/1000, Millipore, AB152) antibody ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µl of a blocking/permeabilization buffer (5% BSA and 0.2% Triton in PBS; BSA from Sigma; TritonX-100 from VWR) was added and cells were incubated for 1h at RT ...
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bioRxiv - Bioengineering 2023Quote: ... The membrane was cut at 25 kDa to separate actin and LC3 bands and primary antibody in 5% BSA blocking buffer (anti-LC3B, rabbit, Sigma-Aldrich L7543 at 1:1000 and anti-actin ...
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bioRxiv - Cell Biology 2024Quote: ... Fixed cells were then incubated for 30 min at 37°C in blocking buffer [0.5% (w/v) bovine serum albumin (BSA, Sigma Cat. #A9647), 10% (w/v ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 µl of each stock was then spotted onto the surface of YPD plate (Yeast Extract 1%, Peptone 2%, Dextrose 2%, Agar 2%) without and with 1µg/mL tunicamycin (Sigma-Aldrich Inc., MI, USA). Plates were then incubated for 48-72 hours at 30°C before capturing their photographs ...