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Citations for Millipore Sigma :
2551 - 2600 of 4506 citations for Recombinant Human CD274 Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Mouse recombinant EphrinB1-Fc chimera (cat # E0653-200UG) and bovine serum albumin (cat #05470-1G) with purity >98 % were from Sigma. Alexa Fuor-594 BSA (cat #A13101) ...
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bioRxiv - Bioengineering 2021Quote: ... Columns were washed with 20 column volumes PBS and recombinant proteins were eluted with 100 mM β-d-lactose (Sigma) prepared in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... standard conditions (ii) with or without recombinant enzyme Agalsidase alpha (5µl/ml) (Takeda) and (iii) with or without wortmannin (75nM) (Sigma-Aldrich). Of note ...
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bioRxiv - Biochemistry 2020Quote: ... The recombinant proteins were overexpressed in E.coli BL21 (DE3) at 17 °C overnight and then purified by Glutathione-agarose (Sigma #G4510). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... Sample was then diluted 8-fold with 50 mM HEPES pH 8.0 and digested by the addition of 5 μg TrypZean recombinant trypsin (Sigma-Aldrich) and incubation for 8 hours at 30 °C ...
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bioRxiv - Plant Biology 2021Quote: ... residual trypsin activity was measured by monitoring the change in absorbance at 247 nm in presence of increasing concentration of recombinant purified Kunitz Trypsin inhibitor (KTI) when incubated with p-toluene-sulfonyl-L-Arg methyl ester (Sigma). To study the inhibitory action of P235 on KTI action ...
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bioRxiv - Plant Biology 2021Quote: ... Substrate specificity of recombinant AtPME2 activity was determined at pH 7.5 and 28°C using commercial citrus pectin (Sigma-Aldrich, DM >85% ...
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bioRxiv - Systems Biology 2020Quote: ... At time 0 medium was removed from cells and recombinant mouse tumor necrosis factor alpha (TNF-α, Sigma-Aldrich, #T7539) was added at concentrations 0-100 ng/mL as 5-minute pulse ...
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bioRxiv - Microbiology 2021Quote: Six-week-old Balb/cN mice were primed subcutaneously either with 30 μg of recombinant SARS-Cov-2 Spike protein (S) or Spike-RBD (RBD) in the complete Freund’s adjuvant (SIGMA-ALDRICH) and boosted three times at three-week intervals with 20 μg of the same antigen in the incomplete Freund`s adjuvant ...
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bioRxiv - Plant Biology 2021Quote: ... substrate specificity assays for recombinant PcIBMS1 and PcIPMS1 were determined with a spectrophotometric end-point assay with DTNB (Sigma-Aldrich). An aliquot of 1-5 μL of the protein preparation was added to an enzyme assay mixture (100 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... and fractions judged by SDS-PAGE to contain the recombinant protein were pooled and desalted by size exclusion chromatography using 30K centrifugal spin column (Millipore).
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bioRxiv - Biochemistry 2022Quote: ... recombinant proteins were purified by anti-c-Myc Antibody Beads (10D11; Wako) or Anti-FLAG M2 affinity gel (Sigma-Aldrich) following the standard protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The fractions containing the recombinant protein were pooled and concentrated through 15 mL 10-kDa MWCO Amicon-Ultra centrifugal filters (Millipore). Protein concentrations were measured by absorbance at 280 nm in nanodrop (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... pFPV-TurboFP650 recombinant plasmids were selected on Trypticase Soya Agar (TSA - BioMérieux) containing 100 µg/mL of carbenicillin (Sigma-Aldrich) and clones which showed a purple color were selected for restriction analysis ...
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bioRxiv - Microbiology 2021Quote: ... The rabbit polyclonal anti‐Per1 antibody was generated against recombinant Per1 protein (recPer1) corresponding to bases 55‐462 of per1 CDS (Aste010406) expressed in pET‐42a (Novagen) commercially (GL Biochem Ltd ...
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bioRxiv - Immunology 2022Quote: ... 20 ng/ml recombinant IL-1β (Preprotech) or 100 ng/ml lipopolysaccharide from Escherichia coli O55:B5 (LPS, Sigma-Aldrich) for 6h at 37°C ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... For capture of antibodies and Fc-Fusions a CM5-S-Series sensor chip (Cytiva) was functionalized with 2500RU recombinant Protein A (Sigma) using EDC/NHS Coupling Kit (Biacore/Cytiva BR-1000-50 ...
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bioRxiv - Developmental Biology 2019Quote: ... The serum-containing mESCs medium was composed of high glucose DMEM (HyClone) supplemented with 1000 U/mL recombinant mouse leukaemia inhibitory factor (LIF) (Millipore), 100 μM non-essential amino acids (NEAA ...
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bioRxiv - Plant Biology 2019Quote: ... using a VP-ITC microcalorimeter (MicroCal) as described [34] after extensive dialysis of the purified recombinant protein in Chelex 100 (Sigma) to remove contaminating divalent cations ...
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bioRxiv - Developmental Biology 2020Quote: ... The trophectoderm was removed by immunosurgery and “epiblasts” were grown in gelatinised dishes in the presence of NDiff + 2i and ESGRO (recombinant mouse LIF Protein, Millipore) to establish ESC lines.
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bioRxiv - Cancer Biology 2021Quote: Primary HPMECs in EBM2 medium or ST1.6R cells in DMEM/1% FBS were cultured overnight and stimulated with 2 μg/ml of recombinant TNC (Merck Millipore) for 24 h ...
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bioRxiv - Cell Biology 2019Quote: ... Immune sera were generated by immunization of 6 weeks-old female NMRI mice (Charles Liver Laboratories) via subcutaneous injection of 100 µg recombinant protein emulsified in Freund’s incomplete adjuvant (Sigma-Aldrich) followed by a boost after 4 weeks with 50 µg of recombinant protein ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were digested for at least 14 h (overnight) with an animal-free recombinant bovine trypsin expressed in corn (Trypzean T3568 from Sigma) at 1/50 w/w and RT ...
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bioRxiv - Microbiology 2020Quote: ... chondrophila were first evidenced by incubating the purified recombinant enzymes overnight at 30°C with 10 mg.mL-1 glycogen from rabbit liver (Sigma-Aldrich) and 20 mM inorganic phosphate in 20 mM Tris-HCl buffer (pH 6.8) ...
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bioRxiv - Microbiology 2021Quote: ... kinetics was recorded as the HA loaded sensors were dipped into 1.5-fold serially diluted concentrations of recombinant GM2 (Sigma Aldrich). The reaction was carried out in a 1x kinetics buffer comprising of 1x PBS ...
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bioRxiv - Physiology 2020Quote: ... tau/NTF or N-terminal (243-441) tau/CTF cDNA was used to produce recombinant tau protein in Escherichia coli BL21 (DE3) (Merck Millipore). Purification was performed as described previously (Hasegawa et al. ...
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bioRxiv - Biophysics 2021Quote: Catalytic activity was evaluated in vitro using 20 pmol of purified recombinant proteins in a 200 µl reaction buffer supplemented with 20 mM p-nitrophenyl phosphate (Sigma) as substrate ...
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bioRxiv - Microbiology 2021Quote: ... Purification of the recombinant mucoricin was performed by Ni-NTA matrix affinity purification according to the manufacturers’ instructions (Sigma-Aldrich). The purity of the protein was confirmed by SDS-PAGE and quantified by a modified Lowry protein assay (Pierce).
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bioRxiv - Molecular Biology 2022Quote: ... and 1 μg of FhNEJ-Teg or recombinant protein in the presence or absence of 15 ng t-PA (Sigma) or 10 ng u-PA (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... we randomly assigned Ai9:Tmem119cre mice to receive an intravenous injection of control solution or 0.05 mg/kg of recombinant IFNγ from mouse (Sigma #I4777), minutes before initiating the rupture of cortical capillaries and then 12h later ...
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bioRxiv - Molecular Biology 2024Quote: ... Further confirmation of purification of the recombinant protein was obtained by immunoblots probed with mouse anti-polyhistidine IgG (Sigma-Aldrich) and goat anti-mouse alkaline phosphatase-conjugated antibodies (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: BMDM were polarized or not with recombinant IL-4 (10 ng mL−1, peprotech) for 24 hours and with LPS (10 ng/ml, Sigma) for 8 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... Jurkat cells were infected with the recombinant viruses in the presence of 8 µg/ml polybrene (hexadimethrine bromide, Sigma-Aldrich) for 15 hours at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... were subcutaneously injected with 100 µg of the pure recombinant protein emulsified in Freund’s incomplete adjuvant (Sigma Aldrich, Taufkirchen, DE) followed by a boost after 4 weeks ...
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bioRxiv - Cancer Biology 2022Quote: ... were cultured in the presence of recombinant mouse IL-4 (25 ng/mL; #404-ML, R&D) and LPS (20 μg/mL; #L2880, Sigma) over night to induce B cell activation and terminal differentiation ...
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bioRxiv - Microbiology 2023Quote: ... All the recombinant viruses were rescued by transfecting BAC DNA into Vero cells using lipofectamine DNA transfection reagent (Millipore-Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... All the recombinant viruses were rescued by transfecting BAC DNA into Vero cells using lipofectamine DNA transfection reagent (Millipore-Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 21 and 42 with 50 μg of recombinant LMW630 mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, MO, USA). Enzyme-linked immunosorbent assay was performed to measure serum responses to antigen (see methods below ...
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bioRxiv - Developmental Biology 2024Quote: Bovine OVGP1 (bOVGP1) and murine OVGP1 (mOVGP1) recombinant proteins were purified using the Amicon Pro Purification System (Merck Millipore Ltd) by combining metal chelation chromatography with an Amicon concentrator membrane ...
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bioRxiv - Systems Biology 2024Quote: ... 60µg of total protein per sample and 5µg recombinant Tau (rTau) (2N4RTau and 1N3R Tau) were digested with either Trypsin Protease (Pierce) or LysargiNase (EMD Millipore) at 1:50 w/w overnight at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... human epidermal growth factor (EGF, 20 ng/ml; Sigma-Aldrich, E9644), human fibroblast growth factor (FGF ...
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bioRxiv - Cancer Biology 2021Quote: ... Human interleukin-2 (IL-2) (H7041) was purchased from Sigma Aldrich. Ficoll-PaqueTM PLUS (17-1440-02 ...
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bioRxiv - Cell Biology 2020Quote: ... 10 ug/ml of Ephrin-A5/Fc (chimera human, Sigma, E0628) and 10 ug/ml of Fc are mixed with 2.5 ug/ ml of Anti-human IgG (Fc specific ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-human MLKL (Sigma-Aldrich M6697; 1:1000/1:200), rabbit anti-RIPK3 (ProSci #2283 ...
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bioRxiv - Immunology 2021Quote: ... with 0.05% human serum albumin (HSA, Sigma-Aldrich, St. Louis, MO) added to the isolation buffer ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibody was diluted in 1% human AB serum (Sigma-Aldrich) in PBS at the concentrations in Table 2 and incubated overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... Forty-five µl of human tubal fluid medium (HTF; Millipore Sigma) containing 4 mg/ml BSA (HTF-BSA ...
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bioRxiv - Developmental Biology 2022Quote: ... and 48 hours later 5 IU human chorionic gonadotropin (hCG, Sigma). Then females were mated with male Hbxip+/- mice overnight ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was isolated from human dermal fibroblasts (106-05A,Sigma) transfected with Dharmacon siRNA cocktails as follows ...
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bioRxiv - Immunology 2021Quote: ... mouse anti-human/mouse SMA-Cy3 (1:200, #C6198, Sigma Aldrich), rat anti-mouse CD35 (1:100 ...