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Citations for Millipore Sigma :
2551 - 2600 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Mitochondrial mass was assessed by measuring citrate synthase activity from 4 million whole cells normalized to protein input (U/mg) in triplicate from three biological replicates using the Citrate Synthase Assay Kit (CS0720, Sigma-Aldrich, St, Louis, MO).
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bioRxiv - Microbiology 2021Quote: The release of inorganic phosphate during 2C-mediated ATPase hydrolysis was measured using the Malachite Green Phosphate Assay Kit (Sigma Aldrich, Zwijndrecht, The Netherlands). Each 50 µL reaction comprised 500 nM recombinant 2C protein and 1 mM ATP in reaction buffer (10 mM HEPES (pH 7.5) ...
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bioRxiv - Immunology 2022Quote: ... Terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick-end labeling (TUNEL) assay was carried out using the ApopTag Peroxidase In Situ Apoptosis Detection Kit (Millipore Sigma, St. Louis, MO); diaminobenzidine (DAB ...
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bioRxiv - Biochemistry 2021Quote: ... Total protein concentration of the supernatant was determined by comparison to a standard curve of bovine serum albumin (BSA) solutions using the bicinchoninic acid (BCA) assay kit (Catalog No. BCA-1, Sigma, St. Louis, MO, USA) and absorbance was read at 562 nm using a BioTek Synergy HT plate reader ...
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bioRxiv - Biochemistry 2021Quote: ... Supernatant was collected and prepared for glycogen measurement alongside a 2 mg/mL glycogen standard using the Sigma glycogen assay kit (Catalog No. MAK016, Sigma, St. Louis, MO, USA) and read using a BioTek Synergy HT plate reader at 570 nm.
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bioRxiv - Immunology 2022Quote: ... sterile filtered and used in a Milliplex assay to measure cytokines and chemokines in each sample using a 5- and 25plex customized kit (Millipore and R&D Systems). Data were analyzed by calculating the average value of the biological duplicates and then the ratio of Mtb treated / control ...
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bioRxiv - Neuroscience 2022Quote: ... Frozen samples were sent to Eve Technologies and analyzed using the Rat Cytokine/Chemokine Array 27-Plex (BioPlex 200 Cytokine Array Assay Kit Source, Millipore Milliplex; Calgary, AB, Canada).
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bioRxiv - Immunology 2023Quote: ... Blood Urea Nitrogen (BUN) was also determined in the same serum samples using a urea colorimetric assay kit (#MAK006, Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Microbiology 2023Quote: LDH activity was quantified in the serum samples using a lactate dehydrogenase activity assay kit (catalog number MAK066, Sigma-Aldrich, St. Louis, MO, USA). The assays were performed according to the manufacturer’s instructions using serum samples diluted 1:10 in 1x Dulbecco’s phosphate buffered saline (1× DPBS ...
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bioRxiv - Physiology 2023Quote: ... and L-citrulline were quantified with HPLC-MS as previously described.49 Arginase activity was assessed with an arginase activity assay kit (Sigma Aldrich, St. Louis, MO) following manufacturer instructions ...
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bioRxiv - Bioengineering 2024Quote: Cell viability on the mouse hindlimb culture was assessed on days 1 and 7 using the XTT cell viability assay kit (Sigma Millipore, Product No. 11465015001, USA). This assay measures metabolic activity as an indicator of cell viability ...
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bioRxiv - Genomics 2022Quote: ... ratio by a Gibson approach with 3h of incubation followed by dialysis for 45 min on a membrane filter (MF-Millipore 0,025 um membrane, Merck). The product was transformed into 10-beta Electrocompetent E.coli (NEB) ...
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bioRxiv - Microbiology 2022Quote: ... lake water samples were prefiltered through a 5.0-μm prefilter membrane to remove debris followed by filtration with a 0.22-μm filter membrane to catch microbes (Millipore Sigma SVLP02500 and GSWP04700, respectively). Multiple 5.0-μm prefilter membranes were used for each water sample as necessary ...
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bioRxiv - Immunology 2020Quote: ... 20µg of protein of each sample was separated on polyacrylamide gels at 160V for 80 minutes and blotted onto PVDF-membranes (Millipore, 80mA/membrane, 90 minutes). After blocking for 2h in Tris-buffered saline with Tween-20 containing 5% nonfat dry milk and/or BSA ...
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bioRxiv - Cell Biology 2024Quote: ... 0.004% (w/v) bromphenol blue) run on a 10% SDS-PAGE and transferred to a PVDF membrane (Immobilon® -FL PVDF membrane, Sigma-Aldrich, cat. #05317). The membrane was blocked with blocking buffer (0.5% Casein ...
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bioRxiv - Cell Biology 2023Quote: ... through electrophoresis at 200 V for 40 min and transferred to a polyvinylidene dilfluoride (PVDF) membrane (Immunobilon™ transfer membrane; Millipore, Bedford, MA, USA) at 100 V for 90 mins ...
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bioRxiv - Genomics 2024Quote: ... ratio by a Gibson approach with 3 h of incubation at 50°C followed by dialysis for 3 h on a membrane filter (MF-Millipore 0.025 μm membrane, Merck) and vacuum concentration ...
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bioRxiv - Genomics 2024Quote: ... and ligated by Gibson with 3 h of incubation at 50°C followed by dialysis for 3 h on a membrane filter (MF-Millipore 0.025 μm membrane, Merck) and vacuum concentration ...
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bioRxiv - Plant Biology 2020Quote: ... After transferring proteins from gels to PVDF membranes (Merck-Millipore) using Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Plant Biology 2020Quote: ... After transferring proteins from gels to PVDF membranes (Merck-Millipore) using Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Plates were sealed with Breathe-Easy® sealing membrane (Sigma) and briefly centrifuged at 1,000 × g for 10 seconds ...
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bioRxiv - Cancer Biology 2021Quote: ... for depletion of membrane cholesterol and soluble cholesterol (Sigma, C8667) (complex of MβCD and cholesterol ...
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bioRxiv - Cancer Biology 2021Quote: ... and were electroblotted to PVDF membranes (Merck Millipore, Darmstadt, Germany). Membranes were probed with specific antibodies (Reagents) ...
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bioRxiv - Cancer Biology 2021Quote: ... Gels were transferred onto an Immobilon-P membrane (IPVH00010, Millipore). Membranes were blocked in 5% milk in TBST for 90 minutes before being placed in a primary antibody solution ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were blocked using 5% bovine serum albumin (Sigma–Aldrich) in Tris-buffered saline with 0,01% Tween followed by overnight incubation with the primary antibodies ...
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bioRxiv - Developmental Biology 2021Quote: ... then transferred onto PVDF membrane (Immobilon-P 0.45 μm, Millipore) either at 25V overnight or at 400mA constant for 90 minutes (both at 4°C and using NuPAGE Transfer Buffer with 20% methanol).
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bioRxiv - Cell Biology 2020Quote: ... Protein was transferred to Immobilon-FL PVDF Membrane (Millipore #IPFL00010) in 1 x Tris/Glycine buffer (Biorad #161-0772 ...
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bioRxiv - Immunology 2021Quote: ... Membranes were then mounted in MOWIOL mounting media (Sigma-Aldrich) and coverslips applied ...
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bioRxiv - Genomics 2020Quote: ... The membrane was stained with Ponceau S solution (Sigma-Aldrich) and the resulting scanned image was used as a loading control ...
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bioRxiv - Microbiology 2021Quote: ... Separated proteins were transferred to PVDF membranes (Millipore, Billerica, MA) and probed with appropriate dilutions of primary antibody followed by appropriate dilutions of horseradish peroxidase-conjugated secondary antibody ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by electroblotting onto an Immobilon PVDF membrane (EMD Millipore). Rabbit polyclonal antibody against TMEM2 was purchased from Sigma Aldrich (SAB2106587) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The plate was covered with a gas-permeable membrane (Sigma) and incubated with orbital shaking in an M200 plate reader (Tecan ...
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bioRxiv - Neuroscience 2020Quote: ... and transferred via electrophoresis to Polyvinylidene difluoride membranes-PVDF (Millipore). Membranes were blocked for 1hour at room temperature in blocking buffer (5%milk in 0.1%triton in PBS/PBST ...
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were blotted on PVDF membrane (Millipore Immobilon-P, #IPVH00010) at 100 V for 1 h at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... followed by transfer on 0.45 μm nitrocellulose membrane (Sigma-Aldrich). Blocking was performed in 2% BSA in PBS for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... transferred onto nitrocellulose membranes (Sigma-Aldrich, St. Louis, MO, USA) and incubated with primary antibodies (Supplementary Table S4) ...
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bioRxiv - Cancer Biology 2022Quote: ... filtered through a 0.45 μm Durapore PVDF Membrane (Millipore SE1M003M00) and added to cell line culture media ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were wet-transferred to PVDF membranes (Immobilon-P, Millipore), blocked in TBS ...
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bioRxiv - Neuroscience 2021Quote: Cells were labeled with cell membrane labeling dye PKH26 (Sigma) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were transferred onto Immobilon-FL PVDF membranes (Millipore, #IPF00010) for 2 h at 30 V on ice ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were blocked in 5% bovine serum albumin (BSA, Sigma) in Tris-buffered saline (TBS ...
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bioRxiv - Molecular Biology 2021Quote: ... and transferred to Immobilon-FL PVDF Membrane (Sigma-Aldrich, IPFL00010). Proteins on the membrane were stained using a Revert 700 Total Protein Stain (LI-COR Biosciences ...
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bioRxiv - Molecular Biology 2020Quote: ... and transferred to a 0.45µm PVDF membrane (Merck Millipore, IPVH00010) with 10mM CAPS (pH 11 ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were transferred to collagen-coated culture membrane (PICM0RG50, Millipore) in 1.2 ml of medium BME-F12 1:1 (41010-026 ...
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bioRxiv - Molecular Biology 2021Quote: ... These membranes were then incubated with mouse anti-MYC (Millipore), rabbit anti-REST (Millipore) ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by transfer onto polyvinylidene fluoride (PVDF) membrane (EMD Millipore). Membranes were blocked with 5% milk in PBS-T and incubated with primary antibody at 4 °C overnight in 5% milk PBST ...
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bioRxiv - Molecular Biology 2020Quote: ... and membranes were blotted with Anti-Flag M2 antibody (Sigma) followed by a IRDye 680RD Goat anti-mouse IgG (Li-COR) ...
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bioRxiv - Cell Biology 2022Quote: ... and proteins were transferred to a nitrocellulose membrane (Merck Millipore). The membrane was then incubated with 5% BSA in Tris-buffered saline with tween 20 (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were then transferred onto a nitrocellulose membrane (Millipore). The membranes were blocked using Odyssey Blocking Buffer (LI-COR ...
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bioRxiv - Molecular Biology 2021Quote: ... transferred to methanol-activated immobilon-FL PVDF membranes (Merck Millipore) by wet transfer (0.2 M glycine ...