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Citations for Millipore Sigma :
2551 - 2600 of 8403 citations for Papain Inhibitor since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... containing protease inhibitors (1.0mM PMSF and 1.0g/mL each leupeptin, aprotinin, and pepstatin) and Phosphatase Inhibitor Cocktail V (EMD Millipore), 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... The p38 MAPK inhibitor SB203580 (#S8307) and ERK inhibitor U0126 (#U120) were purchased from Sigma-Aldrich (St Louis, MO). They were added (20 μM ...
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bioRxiv - Neuroscience 2022Quote: ... containing protease inhibitors (1.0mM phenylmethylsulfonyl fluoride and 1.0g/mL each leupeptin, aprotinin, and pepstatin) and Phosphatase inhibitor cocktail V (EMD Millipore) was (when evaluating protein phosphorylation) ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with phosphatase inhibitors (okadaic acid 1 µM and sodium fluoride 100 mM) and protease inhibitors (Complete cocktail, Sigma). After one-hour incubation at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed once with RNase free PBS/1%BSA and resuspended in PBS/1%BSA+0.2U/ml RNase inhibitor (Protector RNAse inhibitor, Sigma) at a final concentration of 1000 cells/µL ...
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bioRxiv - Cancer Biology 2020Quote: ... The cOmplete protease inhibitor cocktail (CAT# 11697498001) and PhosSTOP phosphatase inhibitor tablets (CAT# 4906845001) were purchased from Millipore Sigma. The siGENOME non-targeting siRNA control pool was purchased from Horizon Discovery (CAT# D-001206-14-05) ...
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bioRxiv - Cell Biology 2020Quote: ... RNA Polymerase inhibitors were added to cells for 24 h at the following concentrations: BMH-21 (RNAPI inhibitor; Millipore) at 1 µM ...
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bioRxiv - Pathology 2021Quote: ... 10 mM Na4P2O7) in the presence of protease inhibitors (completeTM, Mini, EDTA-free Protein inhibitor cocktail tablets, Roche, Sigma). Gut tissue and GDE were homogenized in the lysis buffer and sonicated three time for 30 seconds each at 10 μm amplitude ...
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bioRxiv - Microbiology 2019Quote: ... pH 7.4) lysis buffers supplemented with a protease inhibitor cocktail (cOmplete™, EDTA-free Protease Inhibitor Cocktail, Sigma-Aldrich). Proteins were identified by mass spectrometry as previously described by Springstein et al ...
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bioRxiv - Cancer Biology 2021Quote: Treatments with ATR inhibitor VE-821 (5 μM, Bioconnect, #S8007) and ATM inhibitor KU-55933 (10 μM, Sigma, #SML1109) were started 2 hours before 5-FU administration ...
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bioRxiv - Molecular Biology 2020Quote: ... the cysteine protease inhibitor E64 and the PDE inhibitor zaprinast were all purchased from Sigma-Aldrich (Missouri, United States). The calcium chelator Fluo-4-AM was purchased from Thermo Fisher Scientific (Massachusetts ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1% Triton X-100) plus 0.5 mg/ml bovine serum albumin (BSA) and protease inhibitors (Complete EDTA-free Protease Inhibitor cocktail; Sigma) and the reaction allowed to proceed for 2 h at 4°C ...
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bioRxiv - Cell Biology 2024Quote: Proteins from larval samples were extracted in 300 µL 5% sodium deoxycholate (SDC) in 50 mM HEPES (pH 8.5) containing Roche cOmplete protease inhibitor and PhosSTOP phosphatase inhibitors (Sigma) using a FastPrep-24 bead beater (MP Biomedicals ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were treated with 25 μM EUG and 1 μM TRPV1 inhibitor AMG-517 or 0.5 μM CaN inhibitor cyclosporin A (CsA, Sigma).
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bioRxiv - Biophysics 2023Quote: ... cells were suspended in ORC lysis buffer supplemented with protease inhibitors (cOmpleteTM EDTA-free Protease Inhibitors (Sigma-Aldrich #5056489001) and 0.3 mM PMSF ...
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bioRxiv - Biophysics 2023Quote: ... cells were suspended in MCM lysis buffer supplemented with protease inhibitors (cOmpleteTM EDTA-free Protease Inhibitors (Sigma-Aldrich #5056489001) and 0.3 mM PMSF ...
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bioRxiv - Systems Biology 2023Quote: ... proteasome inhibitor MG-132 (Product No. 474790) and SCD1 inhibitor A939572 (Product No. SML2356) were purchased from Sigma-Aldrich.
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bioRxiv - Cancer Biology 2023Quote: ... A lysis buffer supplemented with phosphatase inhibitor cocktails (BioTools, B15001A/B) and a protease inhibitor (Millipore Sigma, P2714-1BTL) was used to lyse the cells ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with protease inhibitors (1.0mM phenylmethylsulfonyl fluoride and 1.0g/mL each leupeptin, aprotinin, and pepstatin) and Phosphatase inhibitor cocktail V (EMD Millipore). Mechanical tissue disruption was also performed by triturating sequentially through a 200µL pipette tip ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM sodium orthovanadate) and protease inhibitors (1 mM benzamidine and 0.1 mM PMSF, Protease inhibitor cocktail, Sigma-Aldrich)] ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.1% SDS and 50mM Tris pH 8.0) supplemented with protease inhibitor cocktail and phosphatase inhibitor cocktail (all from Sigma-Aldrich). Protein concentration was measured using Bradford assay (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: ... and the animals were resuspended in 4 ml of lysis buffer before the addition of a protease inhibitor cocktail (SIGMAFAST protease inhibitor tablets, Sigma). The animals were pelleted and as much liquid as possible was removed before the samples were frozen as droplets in liquid nitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... slices were washed three times in cold dissociation solution and then transferred to an enzyme inhibitor mix containing trypsin inhibitor (10 mg/mL, Sigma), bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2019Quote: ... Tissues were dissociated in RIPA buffer with protease inhibitor and phosphatase inhibitor with pellet pestle (Sigma-Aldrich, Cat. # Z359947-100EA) followed by sonication for 15 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... 50mM Tris pH 8.0 and cOMPLETE™ Mini protease inhibitor cocktail (#11836153001) and phosphatase inhibitor cocktail 2 and 3 (Sigma) for lysis ...
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bioRxiv - Cell Biology 2021Quote: ... cycloheximide (C4859) and the tankyrase inhibitor IWR-1 (I0161) and the deubiquitinating enzyme inhibitor N-Ethylmaleimide (NEM) (E3876) were from Sigma. The tankyrase inhibitor Az6102 (S7767 ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 1% protease inhibitor and phosphatase inhibitor cocktail 2 and 3 (all from Sigma Aldrich, St. Louis, MO, USA). Samples were centrifuged at 14,000 rpm for 20 minutes at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... N2A cells were first incubated the night prior the electrophysiological measurements with the PKA inhibitor KT5720 and the PKC inhibitor GF-109203 (both from Sigma, dissolved in DMSO and both used at a final concentration of 1 μM) ...
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bioRxiv - Neuroscience 2021Quote: ... with freshly added phosphatase inhibitors (50 mM NaF and 1 mM Na3VO4) and 1% (v/v) mammalian protease inhibitor cocktail (Sigma). Sonication was conducted at 50% output (Q55 Sonicator ...
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bioRxiv - Systems Biology 2021Quote: ... 10 mL of lysis buffer (IBA LifeSciences Buffer W supplemented with 0.5% NP-40 substitute and one tablet of protease inhibitor (cOmplete™ EDTA-free Protease Inhibitor Cocktail, Sigma Millipore) was added to the thawed pellet ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cell lysates were prepared using RIPA lysis buffer containing protease inhibitor cocktail (cOmplete) and phosphatase inhibitor cocktail (PhosSTOP) (Sigma). Cell lysates were pooled from 3 wells for each control and treatment group and equal amounts of protein were subjected to gel electrophoresis ...
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bioRxiv - Neuroscience 2019Quote: ... 150 mM NaCl, 1% Triton-X100, 1X Complete protease inhibitor cocktail [Roche], 1X phosphatase inhibitor cocktails 2 and 3 [Sigma]). Protein concentration was determined using BCA assays (Pierce Biotech).
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bioRxiv - Neuroscience 2019Quote: ... The resulting pellets were further extracted by sonication in 3X volumes of SDS lysis buffer (50 mM Tris-HCl pH 7.4, 2% SDS, 1X EDTA-free Complete protease inhibitor cocktail [Roche] and 1X phosphatase inhibitor cocktail 2 and 3 [Sigma]). Samples were centrifuged at 21,000g for 30 min at 25°C to obtain the Triton-insoluble (SDS-soluble ...
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bioRxiv - Physiology 2021Quote: ... 0.5% Na-deoxycholate and 1% Triton X100) containing protease inhibitors and phosphatase inhibitor cocktail 1 and 2 (1:100, Sigma). Protein lysates were separated on a 4-15% TGX gel (Biorad ...
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bioRxiv - Cell Biology 2022Quote: ... Inhibitors and drugs (etoposide, trimethoprim, hydroxyurea, methyl methanesulfonate MMS, HIV protease inhibitors, E64, chloroquine and artemisinin) were from Sigma-Aldrich, Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2022Quote: ... in 100µl of binding/wash buffer supplemented with protease inhibitor with protease inhibitor cocktail Set I-Calbiochem 1:100 (Cat# 539131, Merck Millipore) and phosphatase inhibitors ...
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bioRxiv - Genetics 2024Quote: ... differentiation of iPSCs was induced through the Wnt modulation method with 7 μM glycogen synthase kinase 3 b (GSK3b) inhibitor CHIR99021 (Selleckchem) and 5 μM inhibitor of WNT production 2 (IWP2) (Sigma). Basal Media was composed of RPMI media supplemented with B27 (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... and a mixture of 1 μM of the complex III inhibitor antimycin A + 0.1 μM of the complex I inhibitor rotenone (Millipore Sigma)] ...
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bioRxiv - Neuroscience 2023Quote: ... and the gamma secretases inhibitor was purchased as Compound E (γ-Secretase Inhibitor XXI) from EMD Millipore (Burlington, MA, USA). Ammonium acetate (Cat# A11450 ...
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bioRxiv - Molecular Biology 2023Quote: ... Penicillin, Streptomycin, LIF and 2i (3 μM Gsk3 inhibitor CT-99021, 1 μM MEK inhibitor PD0325901) and Vitamin C (Sigma) at a final concentration of 100 μg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... Penicillin/Streptomycin, LIF and 2i (3 μM Gsk3 inhibitor CT-99021, 1 μM MEK inhibitor PD0325901) and Vitamin C (Sigma) at a final concentration of 100 μg/mL ...
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bioRxiv - Plant Biology 2024Quote: ... Phosphatase inhibitor (PI-III; in-house, composition resembling Phosphatase inhibitor cocktail 1,2 and 3 from Sigma-Aldrich, St. Louis, USA) for 1 h ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then lysed in ice-cold RIPA buffer supplemented with protease cocktail inhibitor and PhoSTOPTM phosphatase inhibitor (Sigma-Aldrich). The cell lysate was centrifuged at 16600 x g for 20 minutes at 4°C and the supernatant was collected ...
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bioRxiv - Bioengineering 2024Quote: FMC63 CAR T cells were treated with small-molecule inhibitors targeting Actin (inhibitor: Cytochalasin D, Sigma Aldrich catalog no. C8273), Dynamin (inhibitor ...
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bioRxiv - Plant Biology 2020Quote: ... cOmplete protease inhibitor cocktail EDTA-Free (Sigma-Aldrich), 0.1% (v/v ...
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bioRxiv - Plant Biology 2020Quote: ... and phosphatase inhibitor cocktail 3 (Sigma-Aldrich; P0044). After centrifugation at 13,000 rpm for 10 minutes to remove cell debris ...
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bioRxiv - Biochemistry 2020Quote: ... and Phosphatase inhibitor cocktails (SIGMA, P5726 and P0044) were added.
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bioRxiv - Neuroscience 2021Quote: ... pH 7.4) supplemented with protease inhibitor (Sigma-Aldrich) and incubated for 30 min on ice ...
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bioRxiv - Cancer Biology 2021Quote: ... with protease inhibitor cocktail (Sigma, Mendota Heights, MN). Debris were removed by centrifugation at 13,000 xg for 10 minutes at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... fungizone and 10μg/ml soybean trypsin inhibitor (Sigma). Imaging was subsequently performed on the multiphoton microscopy setup described above with a custom-built temperature-controlled stage insert (59 ...