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Citations for Millipore Sigma :
2551 - 2600 of 4702 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... and then moved into 6-well-plates containing digestion medium (DMEM + 10% FBS + 1% amphotericin B (A2942, Sigma)) and 10% liberase (Roche ...
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bioRxiv - Microbiology 2024Quote: ... The plate was then rinsed with PBS and counter-stained with 4′,6-diamidino-2-phenylindole DAPI (Sigma) for 15 min ...
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bioRxiv - Cell Biology 2020Quote: Glomerular DNA was used for PCR using the RedTaq Ready MasterMix (Sigma-Aldrich, St. Louis, USA) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Embryos were treated with RED extract-N-Amp Tissue PCR reagents (Millipore Sigma, Saint-Louis, MO) to purify genomic DNA and perform the genotyping PCR ...
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bioRxiv - Biophysics 2022Quote: ... sub-cloned into the pNKS2 vector) via PCR with custom-designed primers (Eurofins Genomics, Sigma-Aldrich) and PfuUltra II Fusion HS DNA polymerase (Agilent Technologies) ...
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bioRxiv - Immunology 2022Quote: ... All cell lines were verified as mycoplasma free by the LookOut Mycoplasma PCR detection kit (Sigma). SenV Cantell strain was obtained from Charles River Laboratories and used at 200 hemagglutination units/mL (HAU) ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR reactions were run under standard conditions using KOD Hot Start Master Mix (Sigma-Aldrich: 71842). After subsequent ligation with T4 ligase (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... The amplified DNA was cleaned using a GenElute 96 Well PCR Clean-up kit (Sigma-Aldrich) and sent for sequencing.
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bioRxiv - Developmental Biology 2019Quote: ... and then diluted 1:10 for subsequent qRT-PCR analysis using SYBR Green JumpStart Taq (Sigma) on a CFX Connect Real-Time PCR machine (Biorad) ...
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bioRxiv - Molecular Biology 2019Quote: ... and cells were verified as mycoplasma free by the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich). Infectious stocks of a cell culture-adapted strain of genotype 2A JFH1 HCV were generated and titered in Huh-7.5 cells by focus-forming assay (FFA) ...
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bioRxiv - Molecular Biology 2019Quote: ... H69 cells were Mycoplasma free as established using the Lookout Mycoplasma PCR detection kit (Sigma-Aldrich) and authenticated using STR profiling by ATCC before the start of this study (not shown).
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bioRxiv - Biochemistry 2020Quote: ... cerevisiae DNA the coding sequence for Reb1 was amplified by PCR and cloned into pET21b (Novagen) via InFusion cloning (Clontech ...
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bioRxiv - Biochemistry 2020Quote: ... The PCR reaction was carried out using LiTIMFP and LiTIMRP (Table S1. Sigma-Aldrich Co, India) as the forward and the reverse primers ...
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bioRxiv - Neuroscience 2021Quote: ... DNA was extracted from 6 embryos using the Extract-N-Amp Tissue PCR Kit (Millipore-Sigma) and amplified using Amplitaq Gold Taq Polymerase (ThermoFisher) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lines were tested every fortnight for Mycoplasma using LookOut Mycoplasma PCR Detection Kit (Sigma Aldrich). Cell line identity was confirmed using STR profiling.
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bioRxiv - Physiology 2020Quote: ... Genotypes were confirmed from ear notches by using REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich) and primers as previously described12 ...
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bioRxiv - Synthetic Biology 2021Quote: ... the truncated AtCCD7 gene was amplified by PCR and cloned into the pCDFDuet-1 plasmid (Novagen) using NcoI and NotI to yield the plasmid pCDFDuet-trAtCCD7 ...
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bioRxiv - Systems Biology 2021Quote: ... 15 µL from each PCR product were mixed with 5 µL 6x loading dye solution (Sigma) before being loaded onto a 2% (w/v ...
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bioRxiv - Cancer Biology 2020Quote: ... All cultures were routinely tested for mycoplasma contamination using the LookOut Mycoplasma PCR Detection kit (Sigma). To induce differentiation ...
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bioRxiv - Cell Biology 2021Quote: ... RT-qPCR analysis was performed using the KAPA SYBR Fast PCR master mix (Sigma Aldrich, #KK4605) or SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: ... Genomic DNA extracted from the whole embryos (using Extract-N-Amp™ Tissue PCR Kit, Sigma) was used for the genotyping after vasculature imaging.
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bioRxiv - Physiology 2022Quote: ... Total genomic DNA from blood was isolated using REDExtract-N-Amp Blood PCR Kit (Millipore Sigma) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were routinely subjected to mycoplasma testing using the Mycoplasma PCR ELISA kit (Sigma Aldrich, 11663925910) and tested negative.
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bioRxiv - Microbiology 2019Quote: ... Genes or loci of interest were PCR amplified with KOD Xtreme Hot Start polymerase (Millipore Sigma) using the primers listed in Table S6 ...
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bioRxiv - Paleontology 2019Quote: 3µl of bisulfite treated DNA were amplified with the FastStart High Fidelity PCR System (Sigma, 03553400001) using the primers listed in Extended Data Table 12 ...
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bioRxiv - Microbiology 2020Quote: ... RT-PCR on both gDNA and cDNA was performed using the SYBR® Green JumpStart (Sigma) in a 7500 Fast Real Time PCR cycler from Applied Biosystems.
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bioRxiv - Microbiology 2020Quote: ... and the resulting PCR products were ligated into the same sites in plasmid pET-22b(+) (Novagen). An identical strategy was used to generate plasmids pET-YpTatB22-220 and pET-YpTatB22-91 for expressing and purifying full-length or truncated versions of TatB from Y ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were routinely tested for mycoplasma contamination using the LookOut mycoplasma PCR detection kit (Sigma-Aldrich). Cell lines were authenticated by ATCC with no further authentication carried out after purchase.
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bioRxiv - Neuroscience 2021Quote: ... DNA-free isolation was verified by PCR amplification (RedTaq® Polymerase, catalog no. D4309, Sigma-Aldrich). For this purpose ...
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bioRxiv - Microbiology 2020Quote: ... The cell line was tested free of mycoplasma contamination using LookOut Mycoplasma PCR Detection Kit (SIGMA). Cultured cell monolayers were maintained in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Microbiology 2020Quote: ... All cell lines tested negative for mycoplasma using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich).
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bioRxiv - Immunology 2020Quote: ... All cell lines were verified as mycoplasma free by the LookOut Mycoplasma PCR detection kit (Sigma).
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bioRxiv - Molecular Biology 2021Quote: ... All cell lines were verified as mycoplasma free by the LookOut Mycoplasma PCR detection kit (Sigma).
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bioRxiv - Molecular Biology 2020Quote: ... The cells were tested for mycoplasma using the LookOut® Mycoplasma PCR Detection Kit (Sigma, # MP0035), following the manufacturer’s instructions and using the JumpStart™ Taq DNA Polymerase (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... DNA was extracted from 6 embryos using the Extract-N-Amp Tissue PCR Kit (Millipore-Sigma) and amplified using Amplitaq Gold Taq Polymerase (ThermoFisher) ...
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bioRxiv - Microbiology 2022Quote: ... After amplification PCR products were checked on 1% (w/v) agarose gel (Sigma-Aldrich, Darmstadt, Germany) (5 µL of each PCR product mixed with 1 µL 6x loading dye for 50 min at 120 V ...
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bioRxiv - Microbiology 2022Quote: ... Cell lines were tested for mycoplasma using a PCR assay (Venor GeM Mycoplasma Detection Kit, Sigma).
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bioRxiv - Plant Biology 2024Quote: ... PCR-grade H2O and RedTaq Ready Mix according to the manufacturer’s instructions (Sigma-Aldrich, Rehovot, Israel). The amplified products were subjected to electrophoresis on 1.2% agarose gel containing agarose in 0.5 X TBE buffer and were stained with ethidium bromide ...
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bioRxiv - Microbiology 2024Quote: ... The PCR reaction mixture consisted of Kappa Probe Fast qPCR Master Mix (2X) Kit (Sigma-Aldrich), 0.75 μL of primers and 2.5 μL of RNA in a final volume of 10 μL reaction ...
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bioRxiv - Microbiology 2024Quote: ... The second PCR products were cleaned up using SpeedBeads™ magnetic carboxylate (obtained from Sigma Aldrich). Cleaned PCR2 products were quantified using Qubit® dsDNA HS Assay Kit (Life Technologies ...
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bioRxiv - Microbiology 2024Quote: ... All cell lines were confirmed mycoplasma free using the Lookout Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Bioengineering 2024Quote: ... The PCR product was then mixed with 1/3 volume 10 M ammonium acetate (Sigma, A1542) and 2 volume ethanol (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... DNA fragments encoding human Kid and mNeonGreen were amplified by PCR and assembled into pFastbac1 (Novagen) by Gibson assembly as described (42) ...
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bioRxiv - Cell Biology 2023Quote: ... All cell lines were tested every 6-12 months either by PCR (LookOut kit, Sigma/Merck) or Mycostrips (Invivogen ...
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bioRxiv - Genomics 2023Quote: ... The amplified cDNA was purified using the HighPrep PCR Clean-up System (Sigma Aldrich AC-60050) adding a volume of magnetic beads equal to 0.8x the sample volume ...
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bioRxiv - Synthetic Biology 2023Quote: ... Splicing by overlap extension PCR was performed with the KOD Xtreme Hot Start DNA Polymerase (Novagen). Generally ...
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bioRxiv - Cell Biology 2023Quote: TRPM8 sequences were amplified by PCR using the following primers (ordered from Millipore Sigma, Burlington, MA):
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bioRxiv - Microbiology 2023Quote: ... Each 20 µl PCR reaction contained 10 µl of JumpStart RedTaq DNA Polymerase Master Mix (Sigma), 8 µl dH2O ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were regularly tested for mycoplasma contamination with the LookOut mycoplasma PCR detection kit (Sigma, MP0035), using JumpStart Taq DNA Polymerase (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... PCRs were performed in 25 µl with JumpStart RED Accutaq LA polymerase (Sigma-Aldrich, cat# D8045) in the supplied buffer ...