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Citations for Millipore Sigma :
2551 - 2600 of 10000+ citations for 6 cyclopropylpyridazin 3 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: The competitive inhibition of human fumarase activity in the presence of 2-amino-3-phosphonopropionic acid (AP-3, Sigma-Aldrich A4910) was fluorometrically assessed using a coupled enzyme assay ...
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bioRxiv - Biophysics 2021Quote: ... was performed using a pre-annealed DNA-RNA template (DNA45: 5’-TTCTTT ATCTCTTATTTCCTTCTATTTTCCACGCGCCTTCTATTT-3’; RNA13: 5’-[6FAM]-GGCGCGUGGAAAA-3’, Sigma Aldrich). The reaction buffer comprised 25 mM HEPES pH 7.2 ...
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bioRxiv - Synthetic Biology 2020Quote: ... indole-3-propionic acid (order no. 57400) and indole-3-butyric acid (order no. i5386) have been obtained from Sigma-Aldrich. All ligands have been dissolved in 50mM Tris / 300mM NaCl pH8 buffer with if necessary 1% dimethyl-sulfoxide (DMSO) ...
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bioRxiv - Neuroscience 2019Quote: ... KO2 along with Cas9 donor plasmid were used as template DNA and amplified with F 5’-GAAGAAGAATGGCCTGTTCG-3’ and R 5’-GCCTTATCCAGTTCGCTCAG-3’ with KOD Xtreme (Novagen, #71975). Cycling conditions were as follows ...
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bioRxiv - Microbiology 2019Quote: ... we performed growth curves in YPD liquid medium containing different concentrations of Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) in a 96-well plate (Costar ...
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bioRxiv - Microbiology 2019Quote: ... 3×FLAG- and GST-tagged proteins were detected with a mouse monoclonal HRP-conjugated anti-3×FLAG antibody (M2; Sigma-Aldrich) and a rabbit polyclonal anti-GST antibody (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... RNAs were then chemically cross-linked to the membrane during 90 min at 65°C using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) (Sigma Aldrich). Membranes were prehybridized for 30 min in PerfectHyb™ plus (Sigma Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... and Grace Bio-Labs reusable CultureWellTM gaskets (3 mm x 1 mm, well capacity 3-10 ul, Sigma-Aldrich, GBL103250-10EA) were cleaned 3 times sequentially in 100% isopropanol and Milli-Q H2O ...
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bioRxiv - Developmental Biology 2022Quote: ... Medium was refreshed 3 days after seeding and 3 days later cells were fixed with citrate/acetone/3% formaldehyde and stained with Leukocyte Alkaline Phosphatase Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... in 0,5× TBE buffer for 1h at 350 mA of current and EDC-cross-linked for 45 min at 60°C with a solution containing 33 mg/ml of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) (Sigma Aldrich), 11 ng/ul of 1-methylimidazol and 0.46% of HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... with either the wild type Cacna2d2-3’UTR or the Cacna2d2 3’UTR bearing mutations in the miR-423-5p binding sites (Cacna2d2 mut 3’UTR) in HEK 293 cells (Millipore Sigma). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... 53 from the RNAi Consortium (clones TRCN0000087290 and TRCN0000087292 with target sequence 5’-GCGGGAGTATAGTGAGTTTAA-3’ and 5’-CGGACAGTTTGGCACAATCAA-3’, respectively, distributed by Sigma-Aldrich, Merck ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated with HRP-conjugated secondary antibodies (Supplementary Table 1) in 3% dried milk (Marvel) or 3% BSA (Sigma Aldrich) at room temperature for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... DHEA sulfate (5-androsten-3β-ol-17-one-3-sulfate) and testosterone (4-androsten-17β-ol-3-one) were purchased from Sigma Aldrich. 11β-hydroxyandrostenedione (11β-hydroxy-4-androstene-3,17-dione) ...
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bioRxiv - Neuroscience 2022Quote: ... 3 x 10 min 100% EtOH) and cleared (3 x 5 min) in xylene (247642, CAS: 1330-20-7, Sigma Aldrich). Finally ...
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bioRxiv - Physiology 2023Quote: Tissue from the LV of 3 sedentary and 3 exercised mice of each sex was fixed in RNAlaterTM (Millipore Sigma, MFCD03453003), and RNA sequencing was performed by Genewiz from Azenta Life Sciences ...
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bioRxiv - Immunology 2023Quote: ... The immobilized liposomes were completely removed with an injection of 40 mM 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS) (Sigma-Aldrich) (20 μl ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: ... Slides were incubated for 3 minutes in 3% acetic acid and then for 20 minutes in Alcian blue solution (Sigma Aldrich) at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The bacterial DNA was used to amplify the entire 16S region by PCR [5′-AGAGTTTGATCCTGGCTCAG-3′ (forward) and 5′-AAGGAGGTGATCCAGCCGCA-3′ (reverse)] using Expand High-Fidelity Polymerase (Sigma-Aldrich). The PCR products were purified using the QIAquick PCR Purification kit (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: ... a shRNA sequence based on 5’-GCCTAAATGGTCAAGGAGATA-3’ as the sense nucleotide corresponding to the 3’UTR of mouse mDia1 (NM_007858.4; Sigma-Aldrich, Cat# TRCN0000108685) was designed as an oligonucleotide with overhangs (s ...
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bioRxiv - Molecular Biology 2023Quote: ... RNAs were chemically cross-linked to the membrane for 90 minutes at 65°C using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) (Merck, Sigma Aldrich). The membrane was pre-hybridized for 30 minutes in Perfect Hyb plus (Merck ...
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bioRxiv - Plant Biology 2024Quote: ... where 10 seedlings each were incubated 2.5-3 h in the dark at RT with agitation in 3 ml of liquid medium (same as above) supplemented with either 3 µl of 1 mM Concanamycin A (Sigma, C9705) dissolved in DMSO or with 3 µl DMSO alone (control) ...
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bioRxiv - Biochemistry 2023Quote: Two complementary single strand oligonucleotides (5’-GTAATCTGGGCCACCTGCCTGGGAGGA-3’ and 5’-TCCTCCCAGGCAGGTGGCCCAGATTAC-3’) corresponding E2 box44 were Two complementary single strand oligonucleotides (5’-purchased from Sigma-Aldrich. An equal molar ratio of single strand oligonucleotides was mixed and incubated at 95°C for 5 min ...
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bioRxiv - Bioengineering 2023Quote: ... (1) A modification solution was made by dissolving 10 mM of 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC, Sigma-Aldrich Inc.) and 75 mM of N-hydroxysuccinimide (NHS ...
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bioRxiv - Plant Biology 2024Quote: ... The tissue was placed in 99.8% ethanol and LR White resin (at a ratio of 3:1, 1:1, 1:3; Sigma Aldrich, USA), and next in 100% resin LR White ...
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bioRxiv - Genomics 2023Quote: ... then quickly was cut to small pieces (1-3 mm) in petri dish with 3 -5 ml RMPI medium containing 0.05mg/ml TM Liberase (Sigma Aldrich, 5401119001) and 0.02 mg/ml DNAaseI (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... HEK 293FT cells were transfected with 1μg of TRC-pLKO.1-Puro plasmid containing either non-targeting shRNA (5’-CAACAAGATGAAGAGCACCAA-3’) or ATF4-targeted shRNA (5’-GCCTAGGTCTCTTAGATGATT-3’) (Sigma-Aldrich), together with 1 μg mixture of packaging plasmids (pMD2.G and psPAX2 ...
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bioRxiv - Biophysics 2021Quote: ... for alkaline phosphatase and hexokinase and 50 mM MES (pH 6; Sigma-Aldrich) for glucose oxidase and invertase ...
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bioRxiv - Cancer Biology 2021Quote: The C57BL/6 mouse mammary tumor cell line AT3 (Millipore Cat# SCC178, RRID:CVCL_VR89), the mouse melanoma cell line B16F10 (ATCC Cat# CRL-6475 ...
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bioRxiv - Cell Biology 2020Quote: ... then a 6-fold molar excess of Maleimide-PEG8-succinimidyl ester (Sigma 746207) was added ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were counter-stained with DAPI (4’,6-diamidino-2-phenylindole; Sigma-Aldrich) before mounting in Mowiol.
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bioRxiv - Cell Biology 2020Quote: ... First antibodies comprised anti-acetylated α-tubulin (T7451, clone 6-11B-1, Sigma), anti-langerin (ab192027 ...
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bioRxiv - Cell Biology 2020Quote: ... Some sections were additionally labeled with 4′,6-diamidino-2-phenylindole (DAPI, Sigma D9542 ...
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bioRxiv - Cell Biology 2022Quote: ... 500 μM (±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox, Sigma-Aldrich, 238813) and 1 mM sodium azide in PBS to ensure that the biotinylation reaction does not proceed beyond 1 minute ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were separated on 6% polyacrylamide gel and transferred on PVDF membrane (Millipore) using semi-dry blotting ...
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bioRxiv - Immunology 2021Quote: Acetone fixed 6 μm spleen sections were blocked with normal goat serum (Sigma). For detection of germinal centres ...
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bioRxiv - Microbiology 2019Quote: ... Bacteria were grown on 6 mm soda lime glass beads (Sigma, Z265950-1EA) for 72 hours in Lysogeny Broth (LB ...
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bioRxiv - Genetics 2019Quote: ... Counterstaining was performed with either DAPI (4’, 6-diamidino-2-phenylindole dihydrochloride, Sigma) at 1μg/ml ...
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bioRxiv - Developmental Biology 2019Quote: ... diluted at 1:500 and acetylated-tubulin antibody (Sigma-Aldrich, 6-11B-1) at 1:500 ...
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bioRxiv - Developmental Biology 2019Quote: ... Nuclear staining was performed with 4’,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich) diluted 1:4000 in PBS ...
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bioRxiv - Molecular Biology 2019Quote: ... heavy L-lysine-[13C[4/6]15N[0/0]] HCL (Sigma-Aldrich, 643459) and heavy L-arginine-[13C6,15N[0/4]] HCL (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2020Quote: ... Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) (Sigma-Aldrich, St. Louis, MO) served as an antioxidant standard and it was likewise suspended in DMSO at 10 mg/ml.
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bioRxiv - Physiology 2021Quote: ... mice were fasted for 6 hours and then 30% D-glucose (Sigma Aldrich) in sterile water was administered by oral gavage at 2g/kg ...
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bioRxiv - Microbiology 2021Quote: ... 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) and chloramphenicol was from Sigma-Aldrich. Haematoxylin and Eosin stain solution was from Thomas Baker.
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bioRxiv - Cancer Biology 2021Quote: Male C57BL/6 Ku703A/3A and Ku70+/+ littermates were injected with DEN (Sigma) (25 mg/kg i.p. ...
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bioRxiv - Biophysics 2021Quote: ... hirtus culture was concentrated by centrifugation (1121 rpm, 2 min, Sigma 6-10) in an oil test cup ...
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bioRxiv - Bioengineering 2020Quote: ... Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI; 1:500, Sigma). The stained sections were mounted in mowiol (Sigma ...
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bioRxiv - Bioengineering 2020Quote: ... potassium ferrocyanide (K3Fe(CN)6) and H2O2 (30%) were obtained from Sigma-Aldrich. Phosphate buffer saline (PBS ...
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bioRxiv - Neuroscience 2021Quote: Brain samples were embedded in 4-5% agarose (Sigma-Aldrich: 9012-36-6) in 0.1M PB and imaged using serial two-photon tomography (Han et al 2018 ...
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bioRxiv - Neuroscience 2021Quote: ... and 0.1 mg/ml of 40-6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) for 2h at RT ...