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Citations for Millipore Sigma :
2551 - 2600 of 10000+ citations for 6 Fluoro 4 hydroxy 1 7 naphthyridine 3 carbonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... where 10 seedlings each were incubated 2.5-3 h in the dark at RT with agitation in 3 ml of liquid medium (same as above) supplemented with either 3 µl of 1 mM Concanamycin A (Sigma, C9705) dissolved in DMSO or with 3 µl DMSO alone (control) ...
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bioRxiv - Immunology 2023Quote: ... The immobilized liposomes were completely removed with an injection of 40 mM 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS) (Sigma-Aldrich) (20 μl ...
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bioRxiv - Genomics 2023Quote: ... then quickly was cut to small pieces (1-3 mm) in petri dish with 3 -5 ml RMPI medium containing 0.05mg/ml TM Liberase (Sigma Aldrich, 5401119001) and 0.02 mg/ml DNAaseI (ThermoFisher ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated with HRP-conjugated secondary antibodies (Supplementary Table 1) in 3% dried milk (Marvel) or 3% BSA (Sigma Aldrich) at room temperature for 1 h ...
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bioRxiv - Microbiology 2024Quote: ... HEK 293FT cells were transfected with 1μg of TRC-pLKO.1-Puro plasmid containing either non-targeting shRNA (5’-CAACAAGATGAAGAGCACCAA-3’) or ATF4-targeted shRNA (5’-GCCTAGGTCTCTTAGATGATT-3’) (Sigma-Aldrich), together with 1 μg mixture of packaging plasmids (pMD2.G and psPAX2 ...
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bioRxiv - Genetics 2021Quote: ... 3 mM MgCl2 (Sigma, 7786-30-3), 0.1% Tween-20 (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... and EIF4E2 shRNA#3 (sh4EHP#3) (Sigma,). GIGYF2 shRNA#1 (shGIGYF2#1 ...
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bioRxiv - Cancer Biology 2022Quote: ... covered with 3-aminopropyltrimethoxysilane (3-APTS, Sigma) for 3 min for activation ...
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bioRxiv - Neuroscience 2021Quote: ... oxygenated DAB (3-3’-diaminobenzidine, Sigma-Aldrich) was dissolved in 0.1 N HCl at a concentration of 5.4 mg/ml and subsequently diluted ten-fold into sodium cacodylate buffer (pH 7.4 ...
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bioRxiv - Bioengineering 2022Quote: ... 3-mercaptobenzoic acid (3-MBA, Sigma-Aldrich)-capped gold nanoclusters (3-MBA-Au nanoclusters ...
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bioRxiv - Neuroscience 2020Quote: ... 2-(5-Benzo [1,3] dioxol-5-yl-2-tert-butyl-3HImidazol-4-yl)-6-methylpyridine hydrochloride hydrate (SB505124) (Sigma-Aldrich; St. Louis, MO) and implanted subdermally ...
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bioRxiv - Cancer Biology 2020Quote: Tetracycline-inducible CALM-AF10 mouse hematopoietic progenitor cells were cultured in 15% FBS DMEM medium without IL-6 and mCSF and treated with either 4 µg/mL doxycycline (DOX) (Millipore Sigma, Billerica, MA) or DMSO for 3 Days ...
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bioRxiv - Neuroscience 2021Quote: ... Milliplex magnetic bead panel assays (CCL2, CXCL10, IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-6, and IL-10; Millipore Sigma, Burlington, MA) were used as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... mice were gavaged at 4-6 weeks of age with five doses of 100 mg/kg of Tamoxifen (T5648, Sigma, St. Louis, MO) in corn oil (C8267 ...
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bioRxiv - Microbiology 2021Quote: Each pellet was resuspended in 500 μL (samples collected at 0 and 6 hr) or 650 μL (samples collected at 12 and 24 hr) with chilled (4°C) nuclease-free water (Sigma-Aldrich, Gillingham, UK). The resuspensions were frozen overnight at −80°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... spun down again and resuspended the cells in 200 µL of 1X PBS (phosphate buffered saline) with 1.5 μl 4′,6- diamidino-2-phenylindole (DAPI, Sigma-Aldrich, 100 ng/ml). DAPI stains dead cells ...
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bioRxiv - Plant Biology 2020Quote: ... supplemented with 10 g L−1 sucrose and 3 g L−1 phytagel (Sigma, Steinheim, Germany) or 8 g L−1 agar (Winkler ...
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bioRxiv - Microbiology 2020Quote: ... 1 mg ml-1 of 3–5 kDa fluorescein isothio-cyanate (FITC)-dextran (Sigma-Aldrich, Germany) in phenol-red free DMEM/F12 medium (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... The cleaned dishes were incubated with 1 mL of 1% (3-aminopropyl) triethoxysilane (APTES; Sigma Aldrich) in ethanol for 1hour at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were infected with pLKO.1 control or pLKO.1-shZSCAN4 (5’-GAATGCAACAACTCTTGTAATCTCGAGATTACAAGAGTTGTTGCATTCT-3’, Millipore Sigma) and further selected with Puromycin (1ug/ml ...
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bioRxiv - Immunology 2020Quote: ... and S100A9 (rA9) were concentrated using an Amicon Ultra-4 centrifugal filter device with a 3 kDa molecular-weight cutoff (Millipore). The primary sequence ...
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bioRxiv - Microbiology 2019Quote: Methylcobalamin (Vitamin B12 CAS Number 13422-55-4) and L-methionine (CAS Number 63-68-3) were purchased from Sigma and dissolved in water at the highest possible soluble concentrations to prepare stock solution ...
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bioRxiv - Developmental Biology 2020Quote: ... and then incubated with nitro blue tetrazolium chloride/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) substrates (Sigma-Aldrich) in NTM (100mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... and the blotting signals were chemically visualized with either the nitro-blue tetrazolium/5-bromo-4-chloro-3′-indolyphosphate (NBT/BCIP) chromogenic assay (Sigma) or infrared scanner ...
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bioRxiv - Immunology 2021Quote: ... Histone neutralisation experiments were performed via intraperitoneal injection with dialysed and combined a-Histone 3 and a-Histone 4 antibodies (Merck Millipore) or control polyclonal rabbit IgG (BioXCell) ...
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bioRxiv - Genetics 2020Quote: ... Samples were immunoprecipitated with antibodies (5-8 μg) overnight at 4°C followed by incubation for 3 hours with protein G-sepharose beads (Millipore) and washed sequentially ...
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bioRxiv - Genetics 2020Quote: ... and precipitated with antibodies overnight at 4°C followed by incubation for 3 h with protein G Sepharose beads (Millipore). Normal mouse ...
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bioRxiv - Plant Biology 2020Quote: ... plants were incubated overnight at 37°C in GUS buffer (3 mM 5-bromo-4-chloro-3-indolyl-β-D-glucuronide [Duchefa Biochemie, Haarlem, The Netherlands], 0.1% v/v Triton X-100 [Sigma, Steinhaim ...
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bioRxiv - Neuroscience 2019Quote: ... The DNA (3–4 μg/μl) together with the dye Fast Green (0.3 mg/ml; Sigma, St Louis, MO, USA) was injected (~1 μl ...
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bioRxiv - Biochemistry 2019Quote: ... CsgA was concentrated to 1.8 mg/ml using a 3 kDa cut-off spin column at 4ºC (Amicon® Ultra-4, Sigma-Aldrich). The size exclusion chromatography (SEC ...
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bioRxiv - Biophysics 2021Quote: ... was also placed into 7 M Guanidium HCl using three successive concentrations and dilutions using a centrifugal concentrator (3 K MWCO, Amicon Ultra-4, Millipore), and diluted to 2 mg/mL ...
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bioRxiv - Biophysics 2021Quote: ... Free dye was removed from the labeled proteins by successive concentrations and dilutions using a 3 K MWCO centrifugal concentrator (Amicon Ultra-4, Millipore). The labeling efficiency was computed by measuring the concentration of the protein and Cy3 dye using their absorbances at 280 nm and 550 nm ...
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bioRxiv - Bioengineering 2021Quote: ... we incubated them with suspended lentiviral solutions (MOI 3-5) for 24 h with 4 µg/mL polybrene (Sigma-Aldrich) before we replaced the medium with refresh culture medium ...
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bioRxiv - Neuroscience 2021Quote: Synaptosomes were prepared from the striatum or cortex of VGLUT1venus or DAT-Cre eGFP-expressing mice by homogenization in 1ml of ice-cold isosmolar buffer (0.32M sucrose, 4 mM HEPES pH7.4, protease inhibitor cocktail Set 3 EDTA-free (EMD Millipore Corp.)) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in B2 with 2% nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl-phosphate (NBT/BCIP, Sigma) until adequate colour development ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: ... coli DH5α cells were transformed with the resulting pho-lac fusion plasmids and streaked on dual indicator plates containing LB agar with 5-bromo-4-chloro-3-indolyl phosphate disodium salt (X-Phos) (Sigma) at a final concentration of 80μg/ml and 6-chloro-3-indolyl-β-D-galactoside (Red-Gal ...
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bioRxiv - Cancer Biology 2020Quote: ... Percentage of GFP positive cell population was measured at day 3 or day 4 as initial time point using a Guava Easycyte HT instrument (Millipore). GFP% (mCherry% ...
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bioRxiv - Immunology 2022Quote: ... 3 mM 2-mercaptoethanol and the mixture was concentrated to 10 mg/ml (Protein concentration) using an Amicon Ultra-4 filter (3 kDa molecular-weight cutoff, Millipore). The FoxP3ΔN’–DNA complex was then mixed with the reservoir solution (0.1 M Tris-Hcl pH 8.5 ...
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bioRxiv - Biochemistry 2022Quote: ... 0.3% Triton X-100 in PBS) and with primary antibodies overnight at +4 °C (primary Abs: mouse anti-flag –Sigma F1804 ...
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bioRxiv - Microbiology 2021Quote: ... Immunoreactive bands were detected by the addition of BCIP (5-bromo-4-chloro-3-indolylphosphate)-nitroblue tetrazolium solution (Sigma-Aldrich). The reaction was stopped after 2 min by washing the blots with large volumes of deionized water.
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bioRxiv - Physiology 2021Quote: ... was incubated for 2-3 h at 4 °C with 30 µl of FLAG-conjugated beads (anti-DYDDDDDK beads, Sigma) or empty beads (ThermoScientific) ...
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bioRxiv - Bioengineering 2021Quote: ... slides were either treated with SIGMA FAST™ BCIP/NBT (5-Bromo-4-chloro-3-indolulphosphate/Nitro blue tetrazolium, pH 9.5, Sigma) and counterstained with nuclear fast red (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... Loss of Memo was induced by 3 daily intraperitoneal injections with 2mg tamoxifen at age 4 weeks (T5648 Sigma-Aldrich, distributed through Merck ...
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bioRxiv - Plant Biology 2019Quote: ... The total proteins were extracted by using lysis buffer and then incubated for 3 h at 4°C with 20 ul of Anti-HA Affinity Gel (Millipore). The immunoprecipitates were washed five times with lysis buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... Color was developed with freshly prepared 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium (BCIP/NBT; Sigma-Aldrich) and the reaction was stopped by addition of 2 mM ethylenediaminetetraacetic acid (EDTA).
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bioRxiv - Cancer Biology 2021Quote: ... after 72 hours of drug treatment, 20μl of a 5mg/mL 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (M2128-MTT; Sigma-Aldrich, USA) solution was added to each of the wells ...
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bioRxiv - Neuroscience 2019Quote: ... The resulting pellets were further extracted by sonication in 3X volumes of SDS lysis buffer (50 mM Tris-HCl pH 7.4, 2% SDS, 1X EDTA-free Complete protease inhibitor cocktail [Roche] and 1X phosphatase inhibitor cocktail 2 and 3 [Sigma]). Samples were centrifuged at 21,000g for 30 min at 25°C to obtain the Triton-insoluble (SDS-soluble ...
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bioRxiv - Physiology 2021Quote: ... The preparation was bathed in standard locust saline (in mM: 147 NaCl, 10 KCl, 4 CaCl2, 3 NaOH and 10 HEPES buffer; pH = 7.2; chemicals from Sigma-Aldrich).
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bioRxiv - Biochemistry 2021Quote: ... 2-3 mg of sNASP-FLAG-myc and sNASP-Strep-HA extracts were incubated for 3 hours at 4 °C degrees with 50 μl of anti-Flag M2 (A2220, Sigma) or Anti-TwinStrep Mag-Strep beads (2-4090-002 ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing eluted BfNFeoAB were concentrated at 4°C using a 15 mL Amicon 3 kDa molecular-weight cutoff (MWCO) spin concentrator (Millipore). Concentrated protein was then further purified by size exclusion chromatography (SEC ...