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Citations for Millipore Sigma :
2501 - 2550 of 7695 citations for NT proBNP Protein Recombinant since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... to standardize protein loading in Laemmli 2X Concentrate sample buffer (Sigma) and deionized water for gel electrophoresis (Bio-Rad) ...
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bioRxiv - Physiology 2022Quote: ... Total protein was quantified with Bradford reagent (SIGMA, cat. no. B6916) and 25 μg of extract were suspended 1:6 (v/v ...
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bioRxiv - Systems Biology 2022Quote: ... Protein was visualized with Immobilon Western Chemiluminescent HRP Substrate (Millipore; #WBKLS0500) and imaged on a ChemiDoc (BioRad) ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were transferred Immobilon-E PVDF membrane (Merck Millipore, Tullagreen, Ireland) and AGAL was detected by monoclonal anti-Galactosidase alpha antibody (# ab168341 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred to a PVDF membrane (Immobilon-P, Millipore, IPVH00010) at 100 mA overnight ...
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bioRxiv - Bioengineering 2023Quote: ... protein was extracted by using RIPA lysis buffer (20-188, Millipore) supplemented with cOmplete Mini protease and phosphatase inhibitors tablets (Roche) ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... After transferring the proteins to PVDF membranes (Millipore, Etobicoke, ON, Canada) as described ...
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bioRxiv - Biophysics 2024Quote: ... The protein was concentrated with Amicon Ultra-15 centrifugal filters (Millipore) and buffer exchanged to storage buffer (20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2024Quote: ... the proteins were transferred onto PVDF membranes (Millipore, Billerica, MA, USA), the membranes were quenched and proteins were detected using specific antibodies ...
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bioRxiv - Genomics 2024Quote: ... The protein concentration was measured with the Bradford Assay (Sigma-Aldrich). Aliquots of the extracts were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (Amersham/GE Healthcare) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was concentrated using Amicon Ultra centrifugal filter units (Merck Millipore). Protein was then aliquoted ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins in the gel bands were reduced with dithiothreitol (DTT) (Sigma) and alkylated by iodoacetamide (IAA ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted protein was concentrated using Amicon Ultra Centrifugal Filter units (Millipore) and further purified by size exclusion chromatography using a Superdex 75 prepgrade column (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was blotted off with filter paper (Sigma–Aldrich, WHA1001110), and immediately after blotting ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins then transferred to 0.45 µM Immobilon-FL PVDF (Millipore Sigma) after methanol activation ...
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bioRxiv - Neuroscience 2024Quote: ... The proteins were transferred to a PVDF membrane (Millipore, catalog #IPVH00010) at 100V for 30 min in transfer buffer (20% methanol ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies and biotinylated Hyaluronic Acid Binding Protein (HABP, #385911 Millipore) were incubated for 72 hours at 4°C or 24 hours at room temperature ...
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bioRxiv - Physiology 2024Quote: ... Protein was visualized with Immobilon Western Chemiluminescent HRP Substrate (Millipore, #WBKLS0500) and imaged on a ChemiDoc (BioRad) ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins bound to anti-Flag M2 affinity beads (Sigma-Aldrich) were recovered from the supernatants and washed by Flag wash buffer (50 mM NaPi (pH 7.5) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred onto Immobilon-P PVDF membranes (Millipore, Darmstadt, Germany) in 1× Tris Glycine with 20% ethanol ...
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bioRxiv - Cell Biology 2024Quote: ... and the protein concentration was determined with Bradford reagent (Sigma-Aldrich). Lysates were subsequently denatured in Laemmli sample buffer for 5 minutes at 95°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were subsequently transferred to a 0.45 µm PVDF membrane (Millipore). Primary antibodies were used at the following dilutions in 5% Blotting-Grade Blocker (BioRad) ...
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bioRxiv - Developmental Biology 2023Quote: ... protein lysates were prepared in 1× RIPA buffer (Millipore, #20-188) from Drosophila head tissue expressing UAS-FLAG-Dx ...
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bioRxiv - Genetics 2024Quote: ... the proteins were transferred onto a nylon membrane (Immobilon-P, Millipore) and incubated with primary antibodies in a blocking buffer (1ξ PBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... and pellets were resuspended in BugBuster® protein extraction reagent (Millipore). Protein from the lysed cell suspension was then extracted by chloroform/methanol precipitation ...
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bioRxiv - Immunology 2023Quote: ... FLAG-tagged proteins were immunoprecipitated with anti-FLAG M2-agarose (Sigma), following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined by a Bradford assay (Sigma Aldrich; B6916). Proteins were denatured in NuPAGE™ LDS Sample Buffer (4X ...
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bioRxiv - Biochemistry 2023Quote: The lysate was pre-cleared with ∼20µL Protein G agarose (Sigma) per 1mL of lysate at 4°C for 30min ...
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bioRxiv - Biochemistry 2023Quote: ... Ubiquitinated proteins were detected using an anti-FLAG antibody (F7425, Sigma), HA-TAP-Gb1 and endogenous Gb1-4 were detected with anti-HA (16B112 ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-pro-surfactant protein C (SFTPC, 1:1000, AB3786, Millipore), goat anti-SOX9 (SOX9 ...
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bioRxiv - Immunology 2023Quote: ... injection of 30 μg of OVA protein (Grade V; Sigma-Aldrich) adsorbed to 2 mg of aluminum hydroxide (alum ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were blotted onto Immobilon-P PVDF membrane (0.2 µm, Millipore) using a Trans-Blot Semi-Dry transfer Cell (BioRad ...
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bioRxiv - Systems Biology 2023Quote: ... total protein amounts were assayed using Fast Green FCF (Sigma, #F7252) staining solution47 and imaged using LICOR Odyssey 700 nm ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins in gel was visualized with sliver staining kit from Sigma. The gels over 100 KDa (SATB2 molecular weight ...
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bioRxiv - Microbiology 2023Quote: ... lysates were incubated with PureProteome protein A/G magnetic beads (Sigma) for 1-2 h ...
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bioRxiv - Microbiology 2023Quote: ... the protein was transferred onto a polyvinylidene difluoride (PVDF) membrane (Millipore), and proteins were detected by probing with specific antibodies (Table S7 ...
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bioRxiv - Microbiology 2023Quote: ... and then incubated with PureProteome protein A/G magnetic beads (Sigma) for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... along with several dilutions of a SOD1 protein standard (Sigma, S9697) were separated on native 12% Tris-Glycine acrylamide gels (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred to an Immobilon polyvinylidene difluoride (PVDF) membrane (Millipore) using a wet transfer Mini Trans-Blot Cell (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... filtered through a 0.45-µm low-protein-binding Durapore filter (Millipore), and stored at −80°C until use ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were concentrated with a 30 kDa cutoff Amicon concentrator (Millipore), and gel-filtered on a Superose 6 10/30 (GE Healthcare ...
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bioRxiv - Developmental Biology 2023Quote: ... strep tag was removed from the eluted proteins by thrombin (Sigma) digestion overnight at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to the polyvinylidene fluoride (PVDF) membrane (Millipore, USA) which was subsequently fixed with 8% acetic acid for 15 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were visualized using Immobilon Forte Western HRP Substrate (Millipore, WBLUF0500).
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bioRxiv - Biochemistry 2023Quote: ... protein tags were removed by tobaccoetchvirus (TEV) protease (#T4455, Sigma-Aldrich) (1:25 w/w ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... Pure protein solutions were concentrated by centrifugation in Centricon tubes (Millipore).
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bioRxiv - Molecular Biology 2023Quote: Total proteins were extracted with RIPA buffer (Sigma Aldrich, Missouri, USA) supplemented with protease and phosphatase inhibitor cocktail then dosage was carried out with BCA protein quantification kit (Life Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... and then the proteins were transferred to the PVDF membranes (Millipore). After being blocked by the 3% BSA (Sigma ...
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bioRxiv - Physiology 2023Quote: ... Protein concentrations were determined using a bicinchoninic acid assay (Sigma, #BCA1), and the resulting absorbance was read at 562 nm ...
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bioRxiv - Neuroscience 2023Quote: ... The protein coated membrane was blocked with 5% normal serum (Sigma) and 5% skim milk (Sigma ...