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Citations for Millipore Sigma :
2501 - 2550 of 3542 citations for NECAP2 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... 10% FCS and human fibroblast growth factor-2 (5 ng/mL, Sigma-Aldrich, St Louis, MO, USA) at 37 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... The DNA-binding domain of human Timeless (DBD; residues 816-954) was cloned into pRSF-Duet1 (Novagen) with an N-terminal His14-SUMO tag and expressed in E ...
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bioRxiv - Immunology 2020Quote: ... T cells were cultured in Click’s medium supplemented with 10% heat-inactivated human serum (Sigma-Aldrich, H4522), 1% L-glutamine and 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2021Quote: ... and primary human monocytes were treated with 100 ng/ml LPS (O26:B6) (Sigma Aldrich; Cat.# L2654) for 3-4 hrs ...
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bioRxiv - Cell Biology 2019Quote: ... 4H1 mouse monoclonal antibody targeting amino acids 35 to 44 of human ORF1p40,67,68 (Millipore, cat. number MABC1152) was used at 1:15000 dilution ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... mice were further injected with 5 IU (0.1 mL/animal) human chorionic gonadotropin (hCG) (Sigma-Aldrich, USA). Twenty hours after PMSG injection ...
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bioRxiv - Microbiology 2019Quote: ... Parasites were grown in human O+ erythrocytes at 4% hematocrit in complete RPMI1640-HEPES media (Sigma-Aldrich) supplemented with 0.3% L-glutamine ...
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bioRxiv - Microbiology 2019Quote: ... Routine plaque assays were performed in duplicate in the presence of 1% pooled neutralising human serum (Sigma). Single-step infections were performed at moi 5 or 10 pfu/cell and multi-round infections at a moi of 0.005 pfu/cell ...
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bioRxiv - Microbiology 2019Quote: Human foreskin fibroblasts (HFFs) were cultured in minimum essential medium Eagle (MEM; Sigma-Aldrich, St. Louis, Missouri) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: Human HepG2 cell line was grown in Dulbecco’s Modified Eagle’s medium – high glucose (Sigma-Aldrich®, D6429) supplemented with 10% fetal bovine serum (PAN Biotech ...
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bioRxiv - Bioengineering 2019Quote: ... and fibrinogen (FBG; from human plasma, 20 mM sodium citrate-HCl pH 7.4, Lot #3169957, Millipore Sigma). FAM-proteins were prepared with 10 mg of protein reconstituted in 900 μL of 0.1 M PBS and 8-fold molar excess of FAM NHS ester solubilized in 100 μL DMSO ...
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bioRxiv - Immunology 2019Quote: ... human liver tissue was minced with scissors and digested in 5mg/ml pronase (Sigma-Aldrich, P5147-5G), 2.93mg/ml collagenase B (Roche ...
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bioRxiv - Immunology 2019Quote: ... using the MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel/Immunology Multiplex Assay (EMD Millipore #HCYTOMAG-60K): GM-CSF ...
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bioRxiv - Molecular Biology 2019Quote: Culture plates were prepared by coating with 40 µg/ml hyaluronic acid from human umbilical cord (Sigma) and 25 µg/ml type IV collagen from human placenta (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... anti-HOIP antibodies used for the detection of human HOIP (Aviva systems biology, ARP43241_P050, and Sigma, SAB2102031), anti-HOIL-1L antibody (Merck Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... and UAS-human PrP-random and placed the crosses in fly media without the activator RU486 (Sigma). When the adult flies eclosed ...
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bioRxiv - Microbiology 2021Quote: ... Bound antibodies were detected with Peroxidase conjugated goat anti-human kappa light chains antibody (A7164-1ML, Sigma). 100 ul TMB(Tetramethylbenzidine,Biopanda TMB-S-001 ...
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bioRxiv - Cancer Biology 2020Quote: ... and human brain cancer U-87 MG (ATTC no. HTB-14) cells were cultured in DMEM (Sigma) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... fixed and stained with an anti-human cytokeratin 18 antibody (20 µg/ml, clone CK2, Millipore, Germany). Cells expressing human cytokeratin 18 were laser-microdissected (PALM Microbeam system ...
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bioRxiv - Cancer Biology 2020Quote: The human tongue OSCC cell lines (H357, SCC-9 and SCC-4) were obtained from Sigma Aldrich, sourced from European collection of authenticated cell culture ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were transduced with the Lentiviral particles targeting the human CRYM coding sequence (Mission shRNAlentiviral particles, Sigma) and a non-targeting shRNA construct as a control using a spinoculation procedure ...
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bioRxiv - Biochemistry 2020Quote: ... And 1.5 µl/sample in mouse raised antibody for human HA (Sigma-Aldrich, St. Louis, MO, USA) was added to the beads ...
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bioRxiv - Microbiology 2020Quote: ... the Milliplex® Map Non-Human Primate Cytokine Magnetic Bead Panel Kit (EMD Millipore Corporation, Billerica, MA) was used ...
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bioRxiv - Microbiology 2021Quote: ... Human monocyte cell line U937 cells were maintained in Roswell Park Memorial Institute 1640 media (Sigma-Aldrich) supplemented with 10% FCS (Fetal calf serum ...
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bioRxiv - Microbiology 2020Quote: ... 10 ng/ml human FGF7, and DCI (50 nM Dexamethasone, 0.1 mM 8-bromo-cAMP (Sigma Aldrich) and 0.1 mM IBMX (3,7-dihydro-1-methyl-3-(2-methylpropyl)-1H-purine-2,6-dione ...
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bioRxiv - Cancer Biology 2021Quote: ... and then incubated overnight at 4 ℃ with primary antibodies against human Kv1.3 (1: 1000, P4497, Sigma, USA), P-p38 MAPK (1 ...
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bioRxiv - Bioengineering 2020Quote: The hCMEC/D3 cell line derived from human temporal lobe microvessels (hBMECs, EMD Millipore, Burlington, MA, USA) was maintained in EndoGRO Basal Medium (EMD Millipore ...
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bioRxiv - Biophysics 2021Quote: Human Serum Albumin (HSA) (≥ 99% pure, Lot number: SLBT8667) was purchased from Sigma Aldrich (St. Louis, MO) and received as lyophilized power.
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bioRxiv - Biophysics 2022Quote: ... cells were incubated for four days with OF Medium complemented with 10 μg/ml human transferrin (Sigma), 20 nM human insulin (Sigma) ...
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bioRxiv - Microbiology 2022Quote: ... Human BlaER1 cells (a kind gift of Thomas Graf) (29) cells were grown in RPMI (Sigma-Aldrich) supplemented with 10% FCS and 2 mM L-glutamine at 37°C and 5% CO2 ...
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bioRxiv - Developmental Biology 2022Quote: ... HUVECs were suspended in 10 mg/ml fibrin hydrogel (human thrombin, Sigma-Aldrich; bovine fibrinogen, Sigma-Aldrich) and mixed with organoids in un-polymerized matrigel at a 20:1 ratio ...
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bioRxiv - Developmental Biology 2022Quote: ... HUVECs were suspended in 10 mg/ml fibrin hydrogel (human thrombin, Sigma-Aldrich; bovine fibrinogen, Sigma-Aldrich) and mixed with organoids in un-polymerized matrigel at a 20:1 ratio ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with HKSdaFREE and HKGE (AvantBio) and 5 ng/mL human recombinant animal-free KGF (Sigma-Aldrich), 1.64 mM CaCl2 and 50 ug/mL ascorbic acid ...
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bioRxiv - Cell Biology 2022Quote: The chip top channel was emptied and subsequently filled with 300 µg/mL human collagen IV (Sigma) solution in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... They were then overlaid with 10-15 μl of 1 mg/ml Human IgG (Millipore Sigma, I4506) mixed with either 20 μg/ml Goat anti-Mouse IgG Alexa Fluor 405 (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2020Quote: The cathepsin L enzymatic assay was carried out as follows: human liver cathepsin L (EMD Millipore 219402) was activated by incubating at reaction buffer (20 mM sodium acetate ...
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bioRxiv - Microbiology 2020Quote: ... and binding detected using 1 in 25,000 dilution of HRP-conjugated goat anti-human IgG (Sigma-Aldrich).
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bioRxiv - Bioengineering 2021Quote: ... and binding was detected with an anti-human IgG (Fc specific) (1:5000 dilution, Sigma-Aldrich, A0170) or anti-FLAG (1:1000 dilution ...
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bioRxiv - Immunology 2022Quote: ... cells were transduced with lentiviral particles harboring a custom human ORF for MEOX1 (Sigma-Aldrich, Mission TRC3) or empty backbonevectors (mock) ...
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bioRxiv - Developmental Biology 2022Quote: ... followed 48 hours later by 5 IU of human chorionic gonadotropin (hCG; Millipore Sigma, St. Louis, MO). The mice were euthanized 8 hours after hCG treatment and ovaries harvested.
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bioRxiv - Genomics 2019Quote: The MISSION pLKO.1-puro human TDP-43 (TRCN0000016038) and control shRNAs (Sigma Aldrich SHC007 and SHC016) were used to produce lentivirus ...
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: HEK293T human embryonic kidney cells were maintained in high-glucose DMEM medium supplemented with 10% FBS (Sigma) at 37°C in 5% CO2 ...
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bioRxiv - Bioengineering 2019Quote: ... Recombinant human BMP-2 (Pfizer) was reconstituted in a 0.1% solution of rat serum albumin (Sigma-Aldrich) and 4 mM HCl and mixed with alginate ...
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bioRxiv - Cancer Biology 2019Quote: ... and Hs578T cells were additionally supplemented with 0.01 mg/mL human insulin (Sigma-Aldrich, St. Louis, MO). All the cells were grown at 37°C and 5% CO2 ...
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bioRxiv - Developmental Biology 2021Quote: Samples from SC-islets and human islets were chemically fixed with 2.5% glutaraldehyde (EM-grade, Sigma-Aldrich) in 0.1 M sodium cacodylate buffer ...
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bioRxiv - Microbiology 2020Quote: ... Calu-3 (human epithelial lung adenocarcinoma) cells were cultured in Minimum Essential Medium Eagle (MEM, Sigma #M4655) supplemented with 10% FCS ...
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bioRxiv - Microbiology 2019Quote: Hormone secretion was quantified by Luminex assay (Human Metabolic Hormone Magnetic Bead Panel, HMHEMAG-34K, EMD Millipore) according to manufacturer’s standard protocol ...
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bioRxiv - Cell Biology 2020Quote: PC-9 (human lung cancer) cells were cultured in RPMI-1640 medium (Sigma-Aldrich, R8758-500 mL) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2020Quote: ... remaining archived samples were processed using a non-human primate multiplexing bead immunoassay (Milipore-Sigma, Burlington, MA). The kit was run according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Lentivirus based knockdown of human ATG16L1 (5’-ACTGTAGCTTTGCCGTGAATG-3’) was performed using MISSION shRNA constructs (Sigma-Aldrich) and psPAX2 packaging system ...