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Citations for Millipore Sigma :
2501 - 2550 of 4532 citations for Human LACC1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... plasmids were mixed with 2 mg/ml Fast Green (Millipore Sigma, F7252). In all conditions ...
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bioRxiv - Biochemistry 2021Quote: Plasmids were transformed into chemically competent Escherichia coli BL21(DE3) cells (Novagen) and used to inoculate TB medium supplemented with 100 µg/mL of ampicillin or 50 µg/mL of kanamycin for pGEX-6P-1 or pET28b vectors ...
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bioRxiv - Biochemistry 2021Quote: ... Amplified gene drmA was cloned in plasmid pACYCDuet-1 (Sigma-Aldrich: 71147), resulting in a non-tagged protein product ...
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bioRxiv - Cell Biology 2020Quote: ... pLKO.1 puro plasmid containing scrambled sequence was used (Sigma SHC016-1EA). pLKO.1 constructs were co-transfected with psPAX2 (Addgene 12260 ...
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bioRxiv - Biochemistry 2022Quote: Plasmids for sgRNA expression were cloned by annealing synthetic oligonucleotides (Sigma-Aldrich) and ligating them into the appropriate vector ...
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bioRxiv - Microbiology 2022Quote: The C1-07020 gene was cloned into the plasmid pETDuet-1 (Novagen) and then transformed into E ...
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bioRxiv - Bioengineering 2022Quote: ... DogCatcher-NANP18 and SpyCatcher were cloned into expression plasmid pET45(+) (EMD Millipore) for protein production in BL21(DE3 ...
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bioRxiv - Biochemistry 2021Quote: ... Retromer plasmids were co-transformed into BL21(DE3) Rosetta2 pLysS cells (Millipore). Cells were grown to OD600 between 0.8-1.0 and induced for 16-20 hours at 20°C with 0.4 mM IPTG ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmid pET28a(+) was purchased from Novagen (EMD Biosciences, San Diego, CA, USA). Preparation of plasmid pACYCcamAB for coexpression of putidaredoxin reductase (CamA or PdR ...
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bioRxiv - Molecular Biology 2022Quote: ... All plasmids were prepared in Stbl3 or DH5α using MiniPrep (Sigma-Aldrich) or MidiPrep (Macherey-Nagel ...
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bioRxiv - Biochemistry 2019Quote: ... the pRSFMBP vector was constructed based on the plasmid pRSFDuetTM-1 (NOVAGEN) with the replacement of the coding sequence for the His-tag by that of MBP (Maltose Binding Protein ...
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bioRxiv - Neuroscience 2019Quote: ... DNA plasmids mixed in saline (PBS) solution and 0.025% Fast Green (Sigma) were injected through the uterine wall into the lateral ventricle of each embryo ...
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bioRxiv - Developmental Biology 2020Quote: ... Plasmids were transfected in respective iFNP cells cultured in DMEM (Sigma #5796) supplemented with 10% calf serum with 50 U/mL each penicillin and streptomycin ...
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bioRxiv - Synthetic Biology 2019Quote: ... The digested plasmids were checked by agarose gel electrophoresis (1% agarose (Sigma), 1xTris-boric acid EDTA buffer ...
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bioRxiv - Microbiology 2020Quote: ... The resulting plasmid (pBEL1307) was transformed into Rosetta 2 (DE3) cells (Novagen). For expression ...
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bioRxiv - Pathology 2021Quote: ... The lentiviral plasmids (LV01) and lentivirus production were obtained from Sigma-Aldrich. Cells were sorted for GFP expression by flow cytometry and maintained in puromycin (3μg/mL) ...
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bioRxiv - Biochemistry 2021Quote: ... The digested PCR product was cloned into a pET-52b(+) plasmid (Novagen) in frame with an N-terminal Strep-tag II obtaining the B35SSB expression vector pET52b::B35SSB ...
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bioRxiv - Genetics 2021Quote: ... coli using the GenElute Plasmid Miniprep Kit (Sigma-Aldrich, St. Louis, MO) or the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: HEK293T cells transfected with indicated plasmids were lysed using SDC (D6750, Sigma) buffer (4% SDS ...
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bioRxiv - Cell Biology 2021Quote: ... plasmids were transformed into BL21(DE3)pLysE bacteria (Sigma-Aldrich, CMC0015-20X40UL) and grown in TB supplemented with 50 µg/mL kanamycin and 33 µg/mL chloramphenicol at 37 °C until an OD600 of 0.4 was reached ...
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bioRxiv - Developmental Biology 2022Quote: ... Plasmid DNA was mixed with Fast Green FCF (final 0.05%; F7252, Sigma) and injected at 2μg/μl into the cochlear duct using the Wiretroll plunger system (53507-426 ...
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bioRxiv - Genetics 2022Quote: ... Transgenic cells were selected with puromycin (sgRNA plasmids) (1 ng/μl, Sigma) or hygromycin (FIREWach plasmids ...
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bioRxiv - Microbiology 2022Quote: ... NS5 coding sequences were subsequently transferred into expression plasmid pET-15b (Novagen) using standard molecular biology procedures (Supplementary Figure S1) ...
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bioRxiv - Cancer Biology 2024Quote: calf thymus DNA and supercoiled plasmid pUC19 DNA were obtained from Sigma Chemical Company ...
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bioRxiv - Synthetic Biology 2023Quote: ... lac operon amplicon was amplified from the pETDuet-1 plasmid (Novagen 71146) and cloned into the pBBR1-MCS1 plasmid to construct the pBBR1-MCS1-plac plasmid ...
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bioRxiv - Neuroscience 2023Quote: ... and 32.5µg gag-pol packaging plasmid (pCMVΔR8.74) using 10 mM polyethylenimine (Sigma) in OptiMEM for three hours (48) ...
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bioRxiv - Plant Biology 2023Quote: ... ATG3 coding regions were cloned into the pET28a (+) plasmid (Novagen, 69864-3) for expression of the corresponding His-tagged protein ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids were transformed into Escherichia coli BL21 Tuner (DE3) strain (Sigma-Aldrich) or Rossetta DE3 (Novagen ...
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bioRxiv - Microbiology 2023Quote: ... or control plasmid expressing 3xFLAG-tagged TRIM25 through X-tremeGENE9 (Millipore Sigma). Twenty-four hours later ...
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bioRxiv - Immunology 2023Quote: ... 293T cells were transfected with premixed plasmids and PEI (MW25000Da, Sigma, USA) and incubated for 72 h ...
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bioRxiv - Cell Biology 2023Quote: ... transfection agent with 3rd generation packaging plasmids and either shScr (SHC332; Sigma), shYAP (addgene #42541) ...
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bioRxiv - Microbiology 2023Quote: ... This plasmid carries the pRSF1030 replicon from RSFDuet™ (Novagen, Merck Millipore), araC and the araBAD promoter from pBAD/His (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... This plasmid carries the pRSF1030 replicon from RSFDuet™ (Novagen, Merck Millipore), araC and the araBAD promoter from pBAD/His (Invitrogen) ...
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bioRxiv - Cancer Biology 2024Quote: 293T cells were transfected with the modified plasmid using GeneJuice (Novagen 70967) mixed with a combination of the assembled plasmid containing the luciferase tag ...
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bioRxiv - Cancer Biology 2024Quote: ... lentivirus containing plasmids from either non-target short hairpin RNA (Sigma #SHC0020) or shCSPG4 (gene target NM_001897 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... DNA was isolated and purified by GeneEluteTM Plasmid Miniprep kits (Sigma, USA). Presence of the mutations was verified by DNA sequencing (The Applied Genomics Core ...
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bioRxiv - Synthetic Biology 2024Quote: ... These cell suspensions were midiprepped (GenElute HP Plasmid Midiprep Kit, Millipore Sigma), and the final pools of library DNA were used for lentivirus production and titering at the University of San Francisco Viracore ...
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bioRxiv - Microbiology 2020Quote: ... bacteria were incubated with 0.5 µg/mL of chicken anti-human C1qA (Sigma-Aldrich), followed by incubation with PE-conjugated donkey F(ab’)2 anti-chicken (Jackson ImmunoResearch) ...
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bioRxiv - Immunology 2021Quote: ... 0.4µg pCMV-VSV-G and 1.6µg of a lentiviral vector (for human shSUV39H1, Sigma, TRCN0000157251 ...
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bioRxiv - Developmental Biology 2021Quote: ... were soaked overnight with human SDF-1 3.3 μg ml−1 (Sigma-Aldrich, S1577) at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... and 20 ng/mL of recombinant human IL-13 (Sigma-Aldrich, St. Louis, MO) to promote differentiation into M2 macrophages ...
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bioRxiv - Genomics 2021Quote: ... Human HEK293T embryonic kidney cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2021Quote: Human neutrophils were purified from fresh peripheral blood by Histopaque®-1119 (Millipore Sigma) and subsequent discontinuous Percoll® gradient centrifugation (102) ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated with primary antibodies (mouse anti-human synuclein, Sigma S5566 (clone SYN211) for human alpha-synuclein ...
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bioRxiv - Immunology 2022Quote: Human monocytes from 3 donors were culture in 8-well chamber slides (Millipore-Sigma) in SFM ...
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bioRxiv - Immunology 2022Quote: Human monocytes from 3 donors were culture in 8-well chamber slides (Millipore-Sigma) in SFM ...
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bioRxiv - Immunology 2022Quote: Human monocytes from 3 donors were cultured in 8-well chamber slides (Millipore-Sigma) in SFM ...
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bioRxiv - Immunology 2022Quote: Human monocytes from 3 donors were cultured in 8-well chamber slides (Millipore-Sigma) in SFM ...
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bioRxiv - Biochemistry 2020Quote: ... full length human PCNA (accession no. NM182649) was cloned into pETDuet-1 MCS1 (Novagen) Amp+ to obtain 6× His N-terminally tagged protein ...
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bioRxiv - Developmental Biology 2019Quote: ... S4 are anti human Tcf7l2 (N-20, SCBT) and anti-gamma tubulin (T9026, Sigma) HRP coupled secondary antibodies (1/2,000 ...