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Citations for Millipore Sigma :
2501 - 2550 of 8159 citations for CEA Calibrator Grade Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The resolved proteins were transferred onto a PVDF membrane (Millipore) using a semi-dry blotting system (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein was expressed E.coli strain Rosetta 2 (DE3) (Novagen). The cells were grown at 37 °C in Luria-Bertani medium (LB ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein G Mag Sepharose beads (Sigma-Aldrich GE28-9513-79) were washed in cell lysis buffer and blocked in 10% BSA (Jackson ImmunoResearch 001-000-162 ...
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bioRxiv - Cell Biology 2023Quote: ... The eluates were incubated with S-protein agarose beads (Novagen) for 1 h at 4°C and then washed thrice with NETN buffer ...
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bioRxiv - Cell Biology 2023Quote: ... and the protein amounts were determined by BCA assay (Sigma). The proteins were denatured by boiling for 10 min in the DTT-containing sample buffer and separated by 4–20% Mini-PROTEIN TGX gels (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Total protein was visualized using Ponceau S stain (Sigma-Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... The proteins were concentrated using Amicon Ultra Centrifugal Filters (Millipore).
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bioRxiv - Biophysics 2023Quote: ... followed by protein concentration via Amicon Ultra 10 MWCO (Millipore). EPR Spectroscopy of Spin-Labeled Grh1 ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were reduced with 4 mM DTT (Sigma-Aldrich) for 60 min at room temperature and alkylated in the dark using 8 mM iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein content was previously checked with Ponceau (Sigma-Aldrich, #P7170) and membranes (Merck ...
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bioRxiv - Microbiology 2023Quote: ... followed by transfer of proteins to an Immobilon membrane (Millipore) using CAPS buffer (CAPS 10mM ...
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bioRxiv - Neuroscience 2023Quote: ... Total transferred protein was visualized with Ponceau S (Sigma-Aldrich) and incubated overnight in blocking buffer (Intercept Blocking Buffer ...
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bioRxiv - Cell Biology 2024Quote: ... concentrated to 500 µl using an appropriate protein concentrator (Millipore) and further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 column (GE Healthcare ...
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bioRxiv - Plant Biology 2024Quote: ... GST-tagged protein fractions were incubated with thrombin (Sigma, Germany) at 4°C overnight for cleavage of the GST-tag (Step 2 ...
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bioRxiv - Biochemistry 2024Quote: ... and an Atto 647N protein labeling kit (Sigma-Aldrich, 76508) were used ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were detected using the Crescendo Western Blotting substrate (Millipore).
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were detected using the Crescendo Western Blotting substrate (Millipore). The other half of the beads were resuspended in the TRI reagent for subsequent RNA extraction and cDNA synthesis.
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bioRxiv - Cell Biology 2024Quote: ... and recombinant protein production was induced via IPTG induction (Sigma). Cells were lysed with BugBuster HT (Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... the protein was extracted with 1X RIPA buffer (Sigma-Aldrich) supplied with EDTA-free Protease Inhibitor Cocktail (cOmplete™ ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were precipitated by adding 100% trichloroacetic acid (Sigma 76039) to a final concentration of 20% v/v and incubated on ice for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred onto polyvinylidene fluoride (PDVF) membranes (Millipore, IEVH00005). Blocking was performed with 5% non-fat milk dissolved in TBST for 1 hour followed by incubation with primary antibodies PRKN (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2024Quote: ... The resolved proteins were then transferred onto nitrocellulose membranes (Millipore), followed by blocking with 5% BSA (Merck).
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bioRxiv - Cancer Biology 2024Quote: ... and proteins were transferred to PVDF membrane (Millipore, cat. ISEQ00010). The membranes were then blocked with 5% non-fat dry milk (Kroger ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was concentrated using an Amicon filter unit (Millipore) down to 1 mL ...
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bioRxiv - Immunology 2024Quote: ... 30 μl of Protein G Sepharose™ beads (#P3296, Millipore) were added and incubated for 2 hours at 4°C with rotation ...
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bioRxiv - Cancer Biology 2024Quote: ... and surfactant protein-C (SPC, EMD Millipore, #AB3786, AT2 marker). To investigate the role of transferrin ...
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bioRxiv - Microbiology 2024Quote: ... BugBuster Lysozyme (BBL) solution (99% BugBuster Protein Extraction reagent (Millipore) and 1% egg white lysozyme (GoldBio ...
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bioRxiv - Microbiology 2024Quote: ... 6g/L peptone (from casein and other animal proteins, Sigma), 2 mL/L glycerol (Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... BugBuster Lysozyme (BBL) solution (99% BugBuster Protein Extraction reagent (Millipore) and 1% egg white lysozyme (GoldBio ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were concentrated using Amicon Ultra-15 centrifugal filters (Millipore; 10K MWCO for TamA ...
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bioRxiv - Immunology 2024Quote: ... Hydrophobic high protein binding membranes 96-well plates (Millipore Sigma) were hydrated with 40% ethanol ...
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bioRxiv - Immunology 2024Quote: ... Proteins were concentrated using an Amicon spin filter (Millipore Sigma) and filtered through 0.22 µm centrifuge Tube Filters (Costar) ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were detected using a primary α-Flag antibody (Sigma) at a 1:3000 dilution and a secondary goat α-rabbit-HRP antibody (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... The protein was concentrated in 10kDa MWCO centrifugal concentrators (Millipore).
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bioRxiv - Molecular Biology 2024Quote: ... protein lysates were incubated with V5 affinity gel (Millipore A7345) and eluted with V5 peptide (Sigma Aldrich V7754 ...
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bioRxiv - Molecular Biology 2024Quote: ... The resolved proteins were electro-transferred to PVDF membranes (Millipore). The proteins of interest were detected with the specific antibodies indicated in the figure legends ...
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bioRxiv - Molecular Biology 2024Quote: Human recombinant IFNα A protein (Sigma-Aldrich catalog number: IF007) was diluted in 0.1% BSA and added to the cells for 24 hours before further analysis.
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bioRxiv - Biochemistry 2024Quote: ... The protein was concentrated using an Amicon filter unit (Millipore) and further purified by SEC using a Superose® 6 Increase 10/300 GL column equilibrated with buffer C (supplemented with 1 mM EDTA when purifying TgREMIND) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Total protein concentrations were measured using Bicinchoninic acid assay (Sigma).
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bioRxiv - Immunology 2024Quote: ... Protein lysates were first boiled in Laemmli loading buffer (Sigma) for 5 minutes at 95°C ...
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bioRxiv - Genetics 2024Quote: ... Purified antibodies were incubated with magnetic G protein beads (Millipore) and separated from Fab fragments following enzymatic digestion (IdeZ ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred to PVDF Immobilon-FL membranes (Merck Millipore) using the Power Blotter Semi-dry Transfer System (Thermo Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were blotted onto a PVDF membrane (Merck Millipore, IPVH00010) or nitrocellulose membrane (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were reduced by incubation with 5 mM dithiothreitol (Sigma) for 45 min at 60°C ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were blotted to a PVDF membrane (0.45 μm, Millipore) and incubated in a rabbit polyclonal antibody against STOP1 (ABclonal ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred onto Immobilion-FL PVDF membranes (Millipore IPFL00010) at 100V for 60 min for acute astrocytes ...
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bioRxiv - Immunology 2024Quote: ... Proteins were then transferred onto 0.45 μm PVDF membranes (Millipore) for wet transfer using Towbin transfer buffer ...
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bioRxiv - Biochemistry 2023Quote: ... was used to plot elution profiles of the protein standard mix Supelco® 69385 Protein Standard Mix 15-600 kDa (Sigma-Aldrich/Merck, Darmstadt, Germany), and the analyzed protein samples.
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bioRxiv - Bioengineering 2023Quote: ... loaded to a polyacrylamide gel (30 μg of total protein/lane) and after gel electrophoresis and protein transfer to polyvinylidene difluoride (PVDF) membranes (Millipore, cat. no. IPVH00010, Burlington, MA). The membranes were blocked with 5% milk in Tris-buffered saline with 0.1% Tween-20 (TBST ...
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bioRxiv - Molecular Biology 2022Quote: ... 167 mM NaCl) and precleared for 2 h with 250 μL of previously blocked 50% protein-A and protein-G beads (Sigma P-7786 and P-3296 respectively). Blocking was achieved by incubating the beads with 0.5 mg/ml of Ultrapure BSA and 0.2 mg/mL of salmon sperm DNA for 4 h at 4°C ...