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Citations for Millipore Sigma :
2451 - 2500 of 10000+ citations for SpectraDye Antibody Labeling Kit IR700 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Chromatin was precleared with 25 µl of beads for 1.5 h and 10 µg of chromatin were incubated overnight with each antibody: 10 µl Anti-MAFB antibody HPA005653 (Sigma-Aldrich) and 5 µl Anti-GR antibody C15200010-50 (Diagenode) ...
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bioRxiv - Neuroscience 2020Quote: ... the nitrocellulose membranes underwent a 1h blocking step in Rockland buffer before 6h incubation with primary antibodies (for all antibody RRIDS, see Key Resources Table): mouse α-KCC2 (1:1000, Millipore), rabbit α-phospho-KCC2-S940 (1:1000 Aviva Systems Biology) ...
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bioRxiv - Biochemistry 2022Quote: ... One hundred microliters of protein A/G agarose beads were incubated with 5 mg of anti-AGO1(WAKO Chemical, #015-22411), anti-AGO2 antibody (3148, gift from Jay A. Nelson lab) or pan antibody (Sigma, MABE56) in 0.5 mL at 4°C with gentle agitation for 2 h ...
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bioRxiv - Molecular Biology 2022Quote: ... cell surface HS level was examined after staining with anti-HS antibody (10E4, amsbio) at 5 μg/ml and FLAG-tagged enzymes were detected by using anti-FLAG® M2 antibody (Sigma), at 2 μg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... The following commercial primary antibodies were used: rabbit anti-Olig2 antibody and mouse anti-panNav mAb (clone K58/35) purchased from Sigma-Aldrich Chimie ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated overnight at room temperature with a combination of 2 different primary antibodies: a mouse monoclonal anti-PCNA antibody (1:500; Sigma-Aldrich; catalogue number P8825 ...
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bioRxiv - Molecular Biology 2022Quote: ... the accumulated lysates were incubated with protein A/G magnetic beads (Thermo-Fisher) pre-coupled with anti-Flag antibody (M2 monoclonal antibody, Sigma-Aldrich) for 2 h at 4°C with rotation ...
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bioRxiv - Neuroscience 2022Quote: ... The secondary substrate was horse radish peroxidase tagged anti-rabbit secondary antibody and anti-goat secondary antibody (1:2500, EMD Millipore). The bands were detected using a chemiluminescent substrate (SuperSignal West Pico ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were incubated for 1 hour using the following antibodies: 1:500 rabbit anti-H3S10ph (Sigma-Aldrich, Merck, 06-570), 1:500 mouse anticyclin B1 (BD Pharmingen ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then permeabilized in antibody buffer (wash buffer + 0.01% digitonin + 2mM EDTA) and incubated with STAT3 (CST, #9139) or FLAG antibody (Sigma Aldrich #F3165) on nutator overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... OFC and mPFC sections were stained to visualize glial fibrillary acidic protein (GFAP) (primary antibody: mouse anti-GFAP antibody, Sigma-Aldrich; secondary antibody ...
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bioRxiv - Molecular Biology 2024Quote: ... or incubations with species-specific biotinylated antibodies (Table S6; Vector) and then streptavidin–horseradish peroxidase (HRP) antibody (Vector; 1:200) before development with diaminobenzidine (Sigma-Aldrich). Counterstaining was performed using toluidine blue ...
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bioRxiv - Genetics 2024Quote: ... The following primary and secondary antibodies were used as per the mentioned dilutions: anti Acetyl-α-Tubulin Antibody (T7451, Sigma Aldrich), anti GAPDH (1:1000 ...
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bioRxiv - Genetics 2024Quote: The primary antibodies used in this paper were mouse anti-tubulin monoclonal antibody DM1A at 1:50 conjugated to FITC (Sigma-Aldrich), mouse anti-tubulin monoclonal antibody E7 at 1:200 (Developmental Studies Hybridoma Bank) ...
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bioRxiv - Microbiology 2024Quote: ... HRP-conjugated anti-mouse secondary antibody (57404) was purchased from SRL and anti-rabbit secondary antibodies were purchased from Sigma (A8275). Alexa Fluor 488 goat anti-mouse (A11001) ...
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bioRxiv - Microbiology 2024Quote: ... with the same combination of primary and secondary antibodies: mouse anti-acetyl-alpha tubulin antibody clone 6-11-B1 (Sigma⍰Aldrich) at a 1:100 dilution and rabbit anti-HA tag antibody (Cell Signalling Technology ...
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bioRxiv - Microbiology 2024Quote: ... The following mixture of primary antibodies was used: a mouse anti-acetyl-alpha tubulin antibody clone 6-11-B1 (Sigma⍰Aldrich) at a 1:100 dilution and a rabbit anti-HA tag antibody at a 1:50 dilution (Cell Signalling Technology) ...
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bioRxiv - Cell Biology 2023Quote: ... and of PTMs (tyrosinated tubulin: YL ½ antibody, ab6160; rat monoclonal, Abcam; acetylated tubulin: T6793 antibody, clone 6-11B-1, mouse, Sigma-Aldrich; polyglutamylated tubulin ...
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bioRxiv - Developmental Biology 2023Quote: ... bead-bound samples were successively incubated with appropriate amount of primary antibodies against protein-of-interest (CTCF antibody: Millipore, 07-729) in 50µL of CUT&RUN antibody buffer for overnight at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were incubated with primary antibody solution 1:8000 dilution of acetylated α tubulin mouse monoclonal antibody (Sigma Aldrich T7451) and a 1:125 dilution of ift88 rabbit monoclonal antibody (Abcam ab184566 ...
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bioRxiv - Genomics 2023Quote: ... sections were incubated with appropriate secondary antibodies as well as directly conjugated primary antibodies at concentrations according to vendor’s guidelines with DAPI (Sigma Aldrich D9542) at a final concentration of 1μg/ml in Agilent Antibody Diluent Solution for 1 hour at room temperature and washed again three times for 5 minutes with Agilent Wash Buffer Solution ...
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bioRxiv - Plant Biology 2023Quote: ... The membrane was probed with 1:10000 anti-phosphoserine or anti-threonine antibody followed by 1:20000 anti-mouse antibody and detected using Luminata Forte Western reagent (Millipore, USA). Same protocol was followed for in vitro kinase assay using the mutated osbzip1.1 protein ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were then incubated overnight at 4°C with primary antibodies (rabbit anti-AUTS2, 1:200, Atlas Antibodies; rabbit anti-GRIA1, 1:10, Calbiochem; mouse anti-GRIA2, 1:100, Millipore; mouse anti-GRIN2BB ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were blocked with PBS 1x-1% non-fat milk overnight at 4°C and incubated in primary antibody anti-Orco (1:500) and the secondary antibody Goat anti-rabbit IgG HRP conjugate (1:5000 Sigma-Aldrich) for 1 hour each ...
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Molecular identification of ALDH1A1 and SIRT2 in the astrocytic putrescine-to-GABA metabolic pathwaybioRxiv - Neuroscience 2023Quote: ... The cells were then incubated with primary antibodies in blocking solution in the following composition: guinea-pig anti-GABA antibody (1:1000, AB175, Millipore, USA), chicken anti-GFAP antibody (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... Goat anti-IgG rabbit alkaline phosphatase conjugated secondary antibody (A9919) and goat anti-IgG mouse alkaline phosphatase conjugated secondary antibody (A2429) were purchased from Sigma Aldrich.
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bioRxiv - Cell Biology 2023Quote: ... Rinsed membranes were incubated in primary antibody solution (primary antibody reserved in 5% bovine serum albumin, BSA, Sigma, 0.1% (w/v) NaN3 in TBS ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then incubated in primary antibody solution containing Anti-Neurofilament 200 primary antibody produced in rabbit IgG (N4142, Sigma Aldrich) and biotin-conjugated Anti-NeuN primary antibody produced in mouse IgG (MAB377B ...
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bioRxiv - Molecular Biology 2023Quote: ... Wells were washed thrice with PBST and monoclonal primary antibody (anti-his-tagged mouse antibody, 1:5000 diluted, Sigma-Aldrich, USA) was added and incubation was continued for 1 h at RT ...
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bioRxiv - Microbiology 2023Quote: ... Incubation with primary antibodies (1 h, RT) was followed by washing and incubation with secondary antibodies and DAPI (25 µg ml-1, Sigma-Aldrich) (1 h ...
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bioRxiv - Microbiology 2023Quote: ... anti-p24 mouse monoclonal antibody (1: 500; MAB7360, R&D, Minneapolis, MN, USA) and anti-ZCCHC3 mouse monoclonal antibody (1: 500; SAB1408147, Sigma-Aldrich). The following secondary antibodies were used ...
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bioRxiv - Genomics 2023Quote: ... Revelation of the primary antibodies was performed by incubation with goat anti-rabbit Horseradish peroxidase-conjugated secondary antibody (Ref#A0545, Sigma-Aldrick) at 1/10000 ratio in blocking solution for 1 hour at room temperature ...
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bioRxiv - Physiology 2023Quote: ... and incubated in anti-PEMT rabbit polyclonal antibody (1:1000, Bio-techne #NBP1-59580) or anti-α-TUBULIN mouse monoclonal antibody (1:10,000, Millipore-SIGMA #T5168) in 5% dairy milk ...
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bioRxiv - Molecular Biology 2023Quote: ... The secondary antibody was the anti-mouse IgG (whole molecule)–peroxidase antibody produced in rabbit (Sigma; cat# A9044; 1:10000 dilution).
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bioRxiv - Genetics 2023Quote: ... then incubated in the primary antibody cocktail for 48-72 hours (1:1000 dilution of tyrosine hydroxylase mouse monoclonal antibody, MAB318, Millipore Sigma). The secondary antibody was biotinylated horse anti-mouse ...
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bioRxiv - Biochemistry 2023Quote: ... and detected by standard western blotting procedures using rabbit anti-sera against IbpA (Eurofin) as primary antibody and HRP-conjugated anti-rabbit IgG as secondary antibody (Sigma-Aldrich). For IbpACn and IbpAVh detection ...
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bioRxiv - Physiology 2023Quote: Proteins of interest were labeled with well-validated custom and commercial antibodies: Connexin43 (Cx43; mouse monoclonal antibody, Cat. #: MAB3067, EMD Millipore Corp. ...
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bioRxiv - Bioengineering 2023Quote: ... The membrane was submerged in secondary antibody solution (TBS-T + 5% BSA + 1:3000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody (Sigma-Aldrich) and incubated for two hours at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... The sections were stained with primary antibodies diluted in 5% v/v horse serum over night at 4°C: mouse anti- glucagon antibody (1:1000, Sigma-Aldrich), guinea pig anti-insulin (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... rat monoclonal antibody against the N-terminus of DAT (MAB369) and rabbit polyclonal antibody against tyrosine hydroxlase (TH) (AB152) were from EMD Millipore, Rabbit polyclonal antibodies against vacuolar protein sorting-associated protein 35 (VPS35 ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... and analyzed by western blot with anti-SARS-CoV-2 N protein monoclonal antibody (0.5 μg/mL, anti-SARS-CoV-2 N mAb, clone 1G10C4 mAb)42 and anti-β-actin antibody (Sigma-Aldrich, cat ...
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bioRxiv - Evolutionary Biology 2023Quote: ... MH27 mouse monoclonal antibody (from supernatent of cells provided by R. Waterston) was used with rhodamine-conjugated goat anti-mouse secondary antibody (Sigma-Aldrich) to stain fixed animals of different stages.
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bioRxiv - Neuroscience 2023Quote: ... A secondary antibody was incubated at room temperature for 1 hour (Donkey anti-rabbit IgG antibody: Sigma-Aldrich, SAB3700932; 1:100). Fusion protein ProteinA-Tn5 was prepared in-house (plasmid Addgene #124601 ...
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bioRxiv - Physiology 2023Quote: ... blots were stripped of connexin or ZO-1 antibodies and reprobed with an actin-specific monoclonal antibody (Ω-actin, 1:5000, Sigma). Intensities of Cx43 and ZO-1 bands were normalized to those of actin and quantified using the Las-3000 software.
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bioRxiv - Cell Biology 2024Quote: ... Cells were immunostained with rabbit anti-SON antibody (1:200 dilution, Novus) or mouse anti-SRRM2 antibody (1:200 dilution, Sigma Aldrich) for 1h at room temperature followed by 3 times wash with DPBS ...
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bioRxiv - Biochemistry 2023Quote: ... If additional primary antibody incubations were required the membrane was stripped using ReBlot Plus Strong Antibody Stripping Solution (EMD Millipore, 2504), washed and blocked again before being reincubated with primary antibody.
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was then washed with TBS-T and incubated with 1:10000 goat anti-rabbit poly-HRP secondary antibody (Thermo, 32260) and 1:10,000 anti-β-actin-peroxidase antibody (Sigma, A3854) in 5% bovine serum album/TBS-T for 1 hr ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were exposed to either fluorophore-conjugated secondary antibodies (Li-Cor; 1:2000) or HRP-conjugated secondary antibodies (Sigma; 1:3000) in TBST with 5% milk for 30 min (Li-Cor ...
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bioRxiv - Microbiology 2024Quote: ... the cells were incubated with secondary antibody in PBS (1:250) anti-Mouse IgG (whole molecule) –fluorescein isothiocyanate (FITC) antibody produced in goat (Sigma, F9006) in dark for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were washed twice with TN buffer and once with DPBS and then were incubated with primary antibodies (anti-FUS, Bioss Antibodies bs-2980R, anti-FUS, Santa Cruz sc-47711, anti-G3BP1, Sigma GW22382A, anti-G3BP1, Sigma PLA0231 ...