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Citations for Millipore Sigma :
2451 - 2500 of 3712 citations for Serpin A5 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... human cells iso-lated and treated in vitro with 50nM TAM (Sigma-Aldrich, H7904-25G) to induce recombination and shRNA expression ...
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bioRxiv - Cell Biology 2021Quote: ... 32 human Nups were systematically depleted using two different commercially available siRNA pools (Dharmacon; Sigma). siRNAs were transfected into the HeLa cells at a 50 nM final concentration using RNAiMAX (ThermoFisher Scientific) ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were cultured in AIM V (Thermo) supplemented with 5% human serum (Sigma #H3667). PBMCs were stimulated for 2 days with CD3/CD28 T cell activation beads (Thermo # 11131D ...
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bioRxiv - Synthetic Biology 2021Quote: ... 5% Human AB serum and 10 mM neutralized N-acetyl L-Cysteine (Sigma-Aldrich #A9165) supplemented with 30 units/mL IL-2 (NCI BRB Preclinical Repository ...
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bioRxiv - Systems Biology 2020Quote: ... 35mm glass Mat-tek dishes were coated with 10µg/ml Fibronectin (human plasma, Sigma #F0895) for 1 hour ...
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bioRxiv - Physiology 2021Quote: Plasma FGF21 (pg/ml) was measured using a human FGF21 ELISA kit (Millipore, Billerica, MA). In Experiment 2 ...
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bioRxiv - Microbiology 2021Quote: ... a human IgG1 kappa standard purified from myeloma plasma (in duplicates per plate, Sigma-Aldrich) and serum samples (starting at a 1:20 dilution ...
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bioRxiv - Molecular Biology 2020Quote: ... and anti-Human IgG (Fc specific) −Peroxidase antibody produced in goat was from Sigma-Aldrich. Two TNFα-antagonists were purchased ...
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bioRxiv - Cancer Biology 2020Quote: HCT116 human colon adenocarcinoma cell lines were maintained in Dulbecco’s Modified Eagle’s medium (DMEM, Sigma), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Microbiology 2022Quote: ... 500ng of the spike protein was treated with 100ng recombinant human cathepsin-L (Sigma-Aldrich) in sodium acetate buffer (pH 5.5 ...
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bioRxiv - Physiology 2021Quote: ... 2×106 cells/ml sperm were incubated in human tubular fluid (HTF) medium (EMD Millipore) at 37°C ...
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bioRxiv - Cell Biology 2020Quote: Human PBMC were isolated from the peripheral blood of healthy volunteers using Ficoll (Sigma-Aldrich) density centrifugation in Greiner LeucoSep-tubes (Sigma ...
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bioRxiv - Genetics 2022Quote: ... the corresponding cDNA fragment of human FLCN was cloned in the pET-30 vector (Novagen) and the His-tagged fusion protein was expressed in E ...
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bioRxiv - Developmental Biology 2022Quote: ... The COCs were incubated in human tubal fluid (HTF) medium (Millipore Corp; MR-070-D) with appropriate concentration of sperm obtained from 1 hour capacitation ...
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bioRxiv - Microbiology 2022Quote: ... Type V collagen from human placenta (Sigma Type IX, C3657, Sigma-Aldrich, St Louis, MO) was pre-solubilized in aqueous acid and diluted in carbonate coating buffer ...
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bioRxiv - Cell Biology 2019Quote: ... They were coated for 1 hour with 50 μg/mL human plasma fibronectin (Merck Millipore) diluted in PBS and washed three times with PBS.
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bioRxiv - Cell Biology 2020Quote: ... pre-coated with human type IV collagen (Cat# C7521, Sigma Aldrich, St. Louis, MO, USA) with 1 ml of BLEAM in the basolateral chamber (ALI day −2) ...
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bioRxiv - Zoology 2021Quote: C-peptide and glucocorticoid levels were determined using a radioimmunoassay (Human C-Peptide, Merck Millipore, Billerica ...
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bioRxiv - Microbiology 2020Quote: ... and 25 ng/mL of human granulocyte-macrophage colony-stimulating factor (GM-CSF, Sigma-Aldrich) for five days ...
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bioRxiv - Microbiology 2020Quote: Human foreskin fibroblasts (Hff1; ATCC® SCRC-1041™) were maintained in DMEM (Sigma-Aldrich) supplemented with 10% heat-inactivated FBS and 100 U/mL penicillin and 100 µg/mL streptomycin.
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bioRxiv - Cell Biology 2020Quote: ... U2OS cell lines and primary human fibroblasts (MRC5) were cultured in DMEM (D6546, Sigma-Aldrich) with 2 mM L-glutamine ...
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bioRxiv - Neuroscience 2019Quote: Protein lysates were collected from regional human astrocytes in radioimmunoprecipitation assay (RIPA) buffer (Sigma-Aldrich) supplemented with a protease and phosphatase-3 inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Immunology 2019Quote: ... Coverslips were washed with PBS and coated with 3% human serum albumin (HSA; A1653, Sigma) o.n ...
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bioRxiv - Developmental Biology 2020Quote: ... followed 48 hours later by intra-peritoneal injection of human chorionic gonadotropin (hCG) (Sigma-Aldrich) (5 units ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Human liver hepatocellular carcinoma (HepG2) cells were cultured in MEM (Sigma, St. Louis, MO, USA) supplemented with 10% FBS ...
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bioRxiv - Biochemistry 2020Quote: HEK293T (human embryonic kidney) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Sigma D6429) containing 10% FBE ...
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bioRxiv - Neuroscience 2021Quote: ... full length human NPY peptide (10 pmol in 1x PBS, Sigma-Aldrich, Cat. No. N5017), anti-CART antibody (1:500 ...
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bioRxiv - Immunology 2021Quote: ... and subsequently incubated for 10 min in the presence of 5 % human serum (Sigma-Aldrich). Cells were then stained for 30 min on ice by fluorochrome-conjugated monoclonal antibodies (mAbs ...
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bioRxiv - Biochemistry 2021Quote: The human Hsf1 and Hsf1-GFP expression constructs were cloned into a pET vector (Novagen). Hsf1 CS mutant (C35S/C103S/C153S/C373S/C378S ...
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bioRxiv - Immunology 2020Quote: ... Antigen-reactive mAbs were detected with goat anti-human IgG (Fab)-horseradish peroxidase (Sigma-Aldrich) conjugate in 1:5000 PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... Netherlands) and 48 hours later with 7.5 IU recombinant human chorionic gonadotrophin/hCG (Sigma-Aldrich, St ...
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bioRxiv - Cell Biology 2020Quote: Mouse embryonic lungs and human bronchial tissue were fixed overnight in 4% PFA (Sigma; 441244) at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... A standard curve was prepared using human IgG1 kappa purified from myeloma plasma (Sigma-Aldrich). Serial dilutions of the IgG standard (in duplicates ...
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bioRxiv - Immunology 2021Quote: The human lung adenocarcinoma A549 cells (ATCC, Manassas, VA) were grown in DMEM (Sigma-Aldrich) medium containing 10% FBS (Gibco ...
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bioRxiv - Immunology 2021Quote: ... IgM was detected using Goat-anti-human-IgM-HRP (horseradish peroxidase) (1:1,000) (Sigma: A6907) and IgG was detected using Goat-anti-human-Fc-AP (alkaline phosphatase ...
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bioRxiv - Immunology 2020Quote: Fresh or cryopreserved human PBMC’s were isolated from heparinised whole blood by Ficoll gradient (Sigma). Cells were incubated for 1hr (37°C ...
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Preferred endocytosis of amyloid precursor protein from cholesterol-enriched lipid raft microdomainsbioRxiv - Neuroscience 2020Quote: ... Aβ42 levels were measured using a High Sensitivity Human Amyloid β42 ELISA Kit (Millipore, #EZHS42).
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bioRxiv - Developmental Biology 2021Quote: Sectioned tissue derived from regenerated human skin was fixed with 10% formalin solution (Sigma HT5012) for 12 minutes ...
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bioRxiv - Microbiology 2022Quote: ... and 25 µg mL−1 of recombinant human purified E-cadherin (5085, Sigma-Aldrich, USA) in PBS for 1 h at 37 °C ...
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bioRxiv - Genetics 2022Quote: ... Human embryonic samples were fixed in 10% (w/v) neutral-buffered formalin solution (Sigma-Aldrich) and embedded in paraffin wax before sectioning ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μL of goat anti-human IgG (Fc specific)-Peroxidase antibody (1 : 5000 dilution, Sigma) was added and incubated for another 1 h at 37 °C ...
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bioRxiv - Bioengineering 2022Quote: ... Fibrin hydrogels were prepared by combining fibrinogen from human plasma (30 mg/mL Sigma-Aldrich) with thrombin from human plasma (1000 U/mL ...
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bioRxiv - Cell Biology 2022Quote: ... SYBR Green I (10,000× dilution; Cambrex Bioscience) and 0.3 μM human-specific primers (Sigma-Aldrich), as shown in Supplementary Table S1 ...
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bioRxiv - Neuroscience 2022Quote: ... Both human and mouse retinal explants were flat mounted on polytetrafluoroethylene organotypic filters (Millipore-Sigma) with the photoreceptor side down ...
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bioRxiv - Cancer Biology 2024Quote: ... shRNA constructs in a pLKO.1 vector targeting human CHMP5 were purchased from Sigma Aldrich along with 2 control non-targeting shRNAs ...
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bioRxiv - Neuroscience 2022Quote: ... The following primary antibodies were used: Rabbit anti-human Aquaporin 4 (1:100, Millipore, AB3594), Rat anti-human CD44 (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... Akata cells were treated with 10 µg/ml of goat anti-human IgG (Sigma I1886) for 0 ...
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bioRxiv - Neuroscience 2022Quote: ... Two hundred microliters of medium containing 2 μM human insulin (Merck, Sigma-Aldrich Cat# I9278) or medium only was added to the thoraxes for 10 min (the final concentration of insulin was 1 μM) ...
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bioRxiv - Cancer Biology 2024Quote: ... AML cell lines and human primary cells were cultured with RPMI 1640 (R8758 Sigma-Aldrich) medium complemented with 10% FBS ...
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bioRxiv - Synthetic Biology 2024Quote: ... 5% Human AB serum and 10 mM neutralized N-acetyl L-Cysteine (Sigma-Aldrich #A9165) supplemented with 30 units/mL IL-2 (NCI BRB Preclinical Repository ...