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Citations for Millipore Sigma :
2451 - 2500 of 7667 citations for Recombinant Ovine IL4 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were determined by Bradford assay kit (Sigma-Aldrich, B6916). Lysates were mixed with 5 × sample buffer (250 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... stained with 1:200 mouse anti-ubiquitinylated proteins (Sigma #04-263) overnight at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were electro-transferred to a polyvinylidene fluoride membrane (Millipore) using Turbo-Blot Turbo transfer system (settings ...
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bioRxiv - Microbiology 2022Quote: ... HA-tagged proteins were immunoprecipitated with anti-HA agarose beads (Sigma) and processed for Western blotting as previously described.
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bioRxiv - Microbiology 2022Quote: ... affinity purified IcsAp protein was mixed with 0.1 mM DSP (Sigma) in PBS and incubated at room temperature for 30 min ...
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bioRxiv - Synthetic Biology 2022Quote: ... resuspended and lysed in 200 μL Bugbuster protein extraction reagent (Millipore). Following another centrifugation ...
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bioRxiv - Plant Biology 2022Quote: ... the proteins were transferred on a PVDF membrane (Immobilon-P, Millipore) using a wet blotting system ...
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bioRxiv - Plant Biology 2022Quote: ... samples were incubated with 25 μl of protein A beads (Millipore) for 2 h in cold room (100 μL were collected as input) ...
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bioRxiv - Neuroscience 2022Quote: ... Resulting protein lysates were precipitated with trichloroacetic acid (TCA) (Sigma, T0699). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-phosphoepitope SR proteins clone1H4 (Millipore, cat#MABE50, 1:2,000 dilution) were used as primary antibodies ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... as measured by a BCA protein assay kit (Sigma-Aldrich, USA).
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bioRxiv - Plant Biology 2022Quote: ... Proteins were electro-transferred onto Immobilon TM PVDF membranes (Millipore, USA). The membrane was incubated with rabbit anti-C ...
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bioRxiv - Molecular Biology 2023Quote: ... resolved proteins were transferred onto Immobilon-P PVDF membranes (Millipore Sigma) using a Bio-Rad Trans-blot® semi-dry transfer cell ...
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bioRxiv - Plant Biology 2023Quote: ... Protein extracts were then reduced with 10 mM dithiothreitol (D9779, Sigma), followed by alkylation with 30 mM Iodoacetamide (I1149 ...
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bioRxiv - Neuroscience 2022Quote: ... Protein was then transferred onto Infrared PVDF membranes (Millipore, Burlington, MA) and membranes were blocked in Immobilon Blok Solution (Millipore ...
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bioRxiv - Physiology 2022Quote: ... Proteins were visualized using a chemiluminescence (ECL) kit (Millipore, MA, USA). Target protein expression was normalized to β-actin (Sigma-Aldrich ...
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bioRxiv - Physiology 2022Quote: ... Target protein expression was normalized to β-actin (Sigma-Aldrich, #2066) or vinculin (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was pre-cleared with Protein G agarose beads (Sigma) and anti-rabbit IgG (Sigma) ...
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bioRxiv - Microbiology 2022Quote: ... were firstly incubated with the Protein-G agarose (Millipore, 16-266) for 2 hrs ...
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bioRxiv - Bioengineering 2022Quote: ... anti-Fatty acid binding protein 1 (FABP1) (1:50, Sigma-Aldrich) and anti-cytokeratin 20 (CK20 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Protein was concentrated using a 10-kDa Amicon filter device (Millipore) and aliquoted into 1 mg aliquots for further use.
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bioRxiv - Physiology 2022Quote: ... to standardize protein loading in Laemmli 2X Concentrate sample buffer (Sigma) and deionized water for gel electrophoresis (Bio-Rad) ...
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bioRxiv - Physiology 2022Quote: ... Total protein was quantified with Bradford reagent (SIGMA, cat. no. B6916) and 25 μg of extract were suspended 1:6 (v/v ...
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bioRxiv - Systems Biology 2022Quote: ... Protein was visualized with Immobilon Western Chemiluminescent HRP Substrate (Millipore; #WBKLS0500) and imaged on a ChemiDoc (BioRad) ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were transferred Immobilon-E PVDF membrane (Merck Millipore, Tullagreen, Ireland) and AGAL was detected by monoclonal anti-Galactosidase alpha antibody (# ab168341 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred to a PVDF membrane (Immobilon-P, Millipore, IPVH00010) at 100 mA overnight ...
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bioRxiv - Physiology 2024Quote: ... Protein was visualized with Immobilon Western Chemiluminescent HRP Substrate (Millipore, #WBKLS0500) and imaged on a ChemiDoc (BioRad) ...
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bioRxiv - Neuroscience 2024Quote: ... The proteins were transferred to a PVDF membrane (Millipore, catalog #IPVH00010) at 100V for 30 min in transfer buffer (20% methanol ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies and biotinylated Hyaluronic Acid Binding Protein (HABP, #385911 Millipore) were incubated for 72 hours at 4°C or 24 hours at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... Protein extracts were then reduced with 10 mM dithiothreitol (D9779, Sigma) for 5 min at 95 °C ...
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bioRxiv - Microbiology 2024Quote: ... Protein was concentrated with Amicon® Ultracel ® 3K filters (Millipore) for immunization of rabbits by Covance (Denver ...
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bioRxiv - Plant Biology 2024Quote: ... total proteins were detected with anti–HA–peroxidase (Sigma, clone 3F10), anti–FLAG (Sigma ...
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bioRxiv - Plant Biology 2024Quote: ... was immobilised on MagnaChIP™ Protein A magnetic beads (Sigma-Aldrich). Both the wildtype as well as the med25-1 mutant were used for the analysis under normoxic and hypoxic conditions ...
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bioRxiv - Immunology 2023Quote: ... Proteins were transferred to an Immobilon-FL membrane (Sigma Aldrich, USA) using a humid transfer device and 350 mA ...
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bioRxiv - Physiology 2024Quote: ... Protein concentration was determined using Bradford assay (Sigma, Cat. No. B6916). 10 μg from each sample was separated in 10% SDS-PAGE gels ...
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bioRxiv - Physiology 2024Quote: ... Protein concentration was determined using Bradford assay (Sigma, Cat. No. B6916). 35 μg of protein were electrophoretically separated using 8% SDS-PAGE and transferred to PDVF membranes for 2.5 h at 200 mA per membrane using a semi-dry system at 4°C ...
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bioRxiv - Physiology 2024Quote: ... Protein content was determined using a Bradford assay (Sigma-Aldrich, USA).
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bioRxiv - Cell Biology 2024Quote: ... and the protein concentration was determined with Bradford reagent (Sigma-Aldrich). Lysates were subsequently denatured in Laemmli sample buffer for 5 minutes at 95°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins bound to anti-Flag M2 affinity beads (Sigma-Aldrich) were recovered from the supernatants and washed by Flag wash buffer (50 mM NaPi (pH 7.5) ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were subsequently transferred to a 0.45 µm PVDF membrane (Millipore). Primary antibodies were used at the following dilutions in 5% Blotting-Grade Blocker (BioRad) ...
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bioRxiv - Physiology 2023Quote: ... proteins were transferred to polyvinylidene difluoride (PVDF) membranes (Millipore, Burlington, MA) overnight ...
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bioRxiv - Genetics 2023Quote: ... PREPL proteins were immunoprecipitated using anti-FLAG M2 antibody (Sigma Aldrich) and separated by SDS-PAGE ...
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bioRxiv - Genetics 2024Quote: ... the proteins were transferred onto a nylon membrane (Immobilon-P, Millipore) and incubated with primary antibodies in a blocking buffer (1ξ PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... protein lysates were prepared in 1× RIPA buffer (Millipore, #20-188) from Drosophila head tissue expressing UAS-FLAG-Dx ...
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bioRxiv - Immunology 2023Quote: ... FLAG-tagged proteins were immunoprecipitated with anti-FLAG M2-agarose (Sigma), following the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2023Quote: ... and pellets were resuspended in BugBuster® protein extraction reagent (Millipore). Protein from the lysed cell suspension was then extracted by chloroform/methanol precipitation ...
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bioRxiv - Genomics 2024Quote: ... The protein concentration was measured with the Bradford Assay (Sigma-Aldrich). Aliquots of the extracts were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (Amersham/GE Healthcare) ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined by a Bradford assay (Sigma Aldrich; B6916). Proteins were denatured in NuPAGE™ LDS Sample Buffer (4X ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred onto Immobilon-P PVDF membranes (Millipore, Darmstadt, Germany) in 1× Tris Glycine with 20% ethanol ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was concentrated using Amicon Ultra centrifugal filter units (Merck Millipore). Protein was then aliquoted ...