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Citations for Millipore Sigma :
2451 - 2500 of 8002 citations for Recombinant NRG4 Protein GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: Hybridoma supernatants were incubated with Protein G sepharose beads (SIGMA) at room temperature for 4 hrs ...
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bioRxiv - Cell Biology 2022Quote: ... Protein was transferred to a polyvinylidene fluoride membrane (Millipore Sigma) overnight at 4ºC and 50mA current ...
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bioRxiv - Neuroscience 2022Quote: ... Protein bands were visualized using chemiluminescence using ECL (EMD Millipore). Densitometric analysis was performed using ImageJ ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysates were precleared with protein G-sepharose 4B beads (Sigma) and then incubated with anti-HA antibody (HA.11 ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein transfer was performed using an Immobilon-FL membrane (Millipore). To detect tubulin ...
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bioRxiv - Biochemistry 2022Quote: ... Protein reduction and alkylation and digestion with trypsin (Sigma-Aldrich) and LysC (WAKO ...
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bioRxiv - Biochemistry 2022Quote: ... KRAS protein was incubated with alkaline phosphatase beads (Sigma Aldrich) and 20-fold excess nucleotide overnight with gentle rotation at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Samples were prepared by precipitating protein with acetonitrile (Sigma-Aldrich) containing diazepam (Cerilliant ...
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bioRxiv - Developmental Biology 2022Quote: ... proteins were precipitated using 1 mg of gamma-globulins (Sigma) and 2 ml 20% TCA followed by centrifugation at 2,000 r.p.m ...
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bioRxiv - Microbiology 2022Quote: ... Purified proteins were concentrated using Centricon YM-10 columns (Millipore) and the eluants were replaced by storage buffer (50 mM Tris-HCl ...
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bioRxiv - Immunology 2022Quote: ... for protein separation and transferred to PVDF membrane (Millipore, IPVH09120) using Mini Gel Tank and Blot Module (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... lysates were quantified for normalisation (BCA Protein Assay kit, Millipore) and loaded on 10% denaturing SDS-polyacrylamide gels ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were detected using α -His from mouse (Sigma H1029), α-Gfp from mouse ...
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bioRxiv - Biochemistry 2022Quote: ... Specific protein bands were visualized using luminol-based substrates (Millipore) and images obtained using a Fusion FX (Vilber Lourmat) ...
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bioRxiv - Genetics 2022Quote: ... the antibodies against other proteins were obtained from Sigma-Aldrich India ...
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bioRxiv - Neuroscience 2021Quote: ... Protein bands were transferred to polyvinylidene difluoride (PVDF) membrane (Millipore) overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentrations were measured by Bradford test kit (Sigma, B6916) and 10 μg/sample was loaded on a 15% polyacrylamide SDS-PAGE gel (30:1 acrylamide/bis-acrylamide) ...
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bioRxiv - Microbiology 2021Quote: ... filtered by a 0.45 µM low protein binding filter (Millipore) and medium was exchanged three times for Opti-MEM I (1X ...
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bioRxiv - Developmental Biology 2021Quote: ... and protein was transferred to a Immobilon-FL membrane (Millipore), which was incubated with goat anti-h/m GLI3 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... proteins were transferred to Immobilon PVDF 0.45 μm membranes (Millipore). Nonspecific binding was blocked by 5% Bovine Serum Albumin in Tris-buffered saline ...
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bioRxiv - Molecular Biology 2020Quote: Antibody to the GAPDH protein was purchased from Millipore (MAB374). Antibodies to the p15 (sc-612 ...
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bioRxiv - Plant Biology 2021Quote: ... and 50 μl Protein A Agarose/Salmon Sperm DNA (Millipore) at 4°C for 2 h ...
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bioRxiv - Biophysics 2020Quote: ... proteins were electrophoretically transferred onto an Immobilon-P membrane (Millipore). After transfer ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore) using a Bio-Rad transfer system at 90 V for 80 min ...
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bioRxiv - Plant Biology 2021Quote: ... Purified proteins were concentrated by ultrafiltration (Amicon Ultra system, Millipore) and stored at −80 °C ...
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bioRxiv - Cell Biology 2021Quote: ... the proteins were transferred to polyvinylidene difluoride (PVDF) membrane (Millipore). Membranes were blocked in 5 % low fat milk in PBS and then incubated with primary antibodies in 0.1 % Tween- PBS at 4°C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates were treated with benzonase (200 U/mg protein, Sigma) for 5 min and then clarified by centrifugation at 15,000 g for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... and filtered through a 0.45um low protein binding filter (Millipore) to remove debris ...
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bioRxiv - Neuroscience 2021Quote: ... Separated proteins were transferred onto PVDF Immobilon P membrane (Millipore) using a Mini Trans-Blot (Biorad ...
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bioRxiv - Immunology 2020Quote: ... followed by 10 nm gold-conjugated protein A (Sigma-Aldrich). Labeled cells were adhered to poly-L-lysine-coated mica strips and fixed with 0.1% glutaraldehyde ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... β-actin was used as protein loading control (Sigma A2066,1:10000). Secondary antibodies anti-rabbit ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were transferred onto Immobilon-FL PVDF membranes (Sigma Aldrich), blocked Odyssey Blocking Buffer in TBS for 1 hour and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 % Protein G-agarose beads (30 μl) (Millipore, 16-266) were added to lysates after 1 h ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were transferred to an Immobilin-P membrane (Millipore®) at 10 Volts for 1 h in a semi-dry apparatus (Trans-Blot® SD ...
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bioRxiv - Biochemistry 2020Quote: ... Immunoprecipitations using antibodies coupled to protein G-Sepharose beads (Sigma) were as described previously (Levy et al. ...
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bioRxiv - Bioengineering 2020Quote: ... Separated proteins were electro-transferred to PVDF membranes (Millipore, UK) for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... the protein samples were transferred onto a PVDF membrane (Millipore). The transferred PVDF membrane was blocked with 3% bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein spots were stained using Direct Blue (Sigma-Aldrich, Australia), excised and transferred to a flat bottom polypropylene 96-well plate (Corning Life Sciences ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were then transferred to a PVDF membrane (Millipore), blocked with 5% non-fat milk ...
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bioRxiv - Plant Biology 2022Quote: ... Six ml of protein extraction buffer (1 tablet of Sigma Complete™ mini protease inhibitor ...
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bioRxiv - Neuroscience 2022Quote: ... proteins were transferred to Immobilon-P PVDF membranes (Millipore, USA), followed by immunoblotting ...
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bioRxiv - Immunology 2022Quote: ... Proteins were reduced with 5 mM dithiothreitol (DTT) (Sigma-Aldrich) for 30 min at 55 °C and then alkylated by the addition of 10 mM iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... the bound proteins were eluted with 3X FLAG peptide (Sigma). The purity of DNA2 proteins was analyzed by 4–15% gradient SDS–polyacrylamide electrophoresis (SDS–PAGE ...
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bioRxiv - Molecular Biology 2022Quote: Protein lysates were generated by incubation with RIPA Buffer (Sigma) supplemented with 1% complete protease inhibitor cocktail (Sigma ...
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bioRxiv - Biophysics 2022Quote: ... FN3 proteins were expressed in Rosetta DE3 cells (EMD Millipore), lysed by sonication ...
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bioRxiv - Cancer Biology 2022Quote: Protein was extracted using Radioimmunoprecipitation Assay (RIPA) buffer (Sigma- Aldrich) supplemented with 1 mM EDTA ...
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bioRxiv - Neuroscience 2022Quote: ... astrocytic marker glial fibrillary acidic protein (GFAP) (chicken, Millipore, AB5541), microglial marker ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Plant Biology 2019Quote: ... before capture of immune complexes with Protein A agarose (Millipore). Crosslink reversal and protein removal was performed as described previously (Nelson et al. ...
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bioRxiv - Plant Biology 2019Quote: ... After transferring proteins from gels to PVDF membranes (Merck-Millipore) using Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2019Quote: ... and purified by protein A-agarose beads (16-157; Millipore). NIPBL ChIP-seq were from recently published data [46] ...