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Citations for Millipore Sigma :
2451 - 2500 of 10000+ citations for Recombinant Mouse Efna1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Mouse IZ-actin (Clone AC-74) and mouse FLAG M2 F3165-1MG were obtained from Sigma-Aldrich and used at 1:10 000 and 1:2 000 dilutions in western blot ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibody (Mouse Anti-Influenza A NP, Millipore MAB8251 or Mouse Anti-Influenza B NP, Millipore MAB8661) was diluted 1:4000 in antibody diluent ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse anti-α-tubulin (1:1000; CST3873) and mouse anti-IDH1 (R132H) (1:500, SAB4200548, Sigma-Aldrich).
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bioRxiv - Developmental Biology 2020Quote: ... individual components can be added to 500 ml media bottle at the indicated final concentrations: 1) Recombinant Human Insulin (Sigma I-1882) – 12.5 µg/ml final concentration ...
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bioRxiv - Molecular Biology 2021Quote: ... The pelleted cells were lysed and used to purify the recombinant proteins with glutathione–Sepharose 4B beads (Sigma Aldrich #GE17-0756-01). The purified protein or whole cell lysate was incubated with the respective agarose beads at various time points with continuous agitation and washed before imaging ...
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bioRxiv - Cell Biology 2022Quote: ... were used as a substrate for phospho-PKCα in a 25 µl in vitro kinase reaction using 100 ng of active recombinant PKCα enzyme (Millipore-Sigma, MA), 5 µl of a lipid activator (Millipore-Sigma ...
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bioRxiv - Biophysics 2020Quote: Both unlabeled and 15N labeled recombinant full-length αS was expressed and purified in Rosetta™ 2(DE3) pLysS (EMD Millipore®) cells using IMPACT™ system protocol (New England Biolabs® ...
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bioRxiv - Evolutionary Biology 2022Quote: The enzyme assay for the zebrafish Irg1-like was performed by incubating 7 µg recombinant protein with 1 mM cis-aconitate (Sigma A3412-1G) in 300 µl reaction buffer (10 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... The organoids were then allowed to grow for 5 days followed by 24 hour treatment with recombinant human protein TNF-α (H8916, Sigma Aldrich) at 40ng/ml or IFN-gamma (PHC4031 ...
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bioRxiv - Microbiology 2020Quote: ... 0.1 ng/ml human recombinant epithelial growth factor and 50 U/ml penicillin and 50 U/ml streptomycin (Sigma-Aldrich, MO, USA). The VK2 cells were seeded into 24-well tissue culture plates ...
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bioRxiv - Microbiology 2021Quote: ... were immunized intraperitoneally with 100 µl of a 1:1 emulsion containing 50 µg recombinant protein LukS or LukF and incomplete Freund’s adjuvant (Sigma-Aldrich, Taufkirchen, Germany). Mice were boosted with an emulsion of protein and incomplete Freund’s adjuvant at day 14 and 28 and bled after six weeks ...
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bioRxiv - Developmental Biology 2022Quote: ... containing the same supplements) freshly supplemented with 25 ng/mL of human recombinant FGF4 (prepared in house) and 1 μg/mL heparin (Sigma-Aldrich, USA). TSCs were passaged every 3-5 days ...
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bioRxiv - Cell Biology 2022Quote: Recombinant His6-EGFP used in the GFP intensity calibration (Figure 1B) was expressed in Rosetta 2 (DE3) competent cells (Millipore Sigma #71400) using pDual-EGFP plasmid (Addgene ...
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bioRxiv - Immunology 2024Quote: ... MASP-3 and MAP-1 was measured in an ELISA assay where 10 µg/ml recombinant human albumin (rHA) (Sigma-Aldrich, A9731) was coated on Nunc MaxiSorp flat-bottom 96-well plates (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... Serial dilutions from 2μg to 0.0625μg of recombinant SPARC (SRP3159, Sigma-Aldrich; S5174-25UG, Sigma-Aldrich; 942-SP-050, R&D Systems) were spotted and used as standards ...
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bioRxiv - Immunology 2024Quote: ... The coating of the Ibidi® µ-slides IV 0,4 Uncoated (Cat# 80601) with fibrin was performed by adding PBS mixed with recombinant human albumin (Sigma-Aldrich, Cat# A9731), fibrinogen from human plasma AF488 Conjugate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: The custom recombinant antibody-like anti-MAR binding reagent (anti-MAR) was generated and purified in-house (now available from Millipore Sigma, MABE1076) (Gibson et al. ...
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bioRxiv - Biochemistry 2023Quote: We performed the phosphorylation kinetics presented in Figure 4a using commercial recombinant kinases GST-p38βat 10 μg/mL (Sigma-Aldrich, ref. B4437), GST-Erk2 at 20 μg/mL (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: BCBL-1/Cas9 cells containing pLenti SpBsmBI Guide Hygro (AAVS1 or TRAIL-R1 #1/2) were treated with the indicated serial dilutions of recombinant human TRAIL (Sigma-Aldrich, T9701) and absolute cell numbers were measured 24 hours later by flow cytometry as described above for cumulative growth curves.
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bioRxiv - Cancer Biology 2024Quote: TKO and QKO mESC were cultured in Serum/LIF media (DMEM-KO, 15% FBS, 1000 U/ml murine recombinant LIF (Millipore Sigma #ESG1106), 1× nonessential amino acids (100x ...
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bioRxiv - Molecular Biology 2024Quote: ... insulin-like growth factor 1 (IGF-1; 100 ng/ml; recombinant Human LONG R3 IGF-1, Sigma-Aldrich, Cat# I1271, MO, USA), rapamycin (10 ng/ml ...
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bioRxiv - Microbiology 2023Quote: ... were transformed with the lithium acetate method 45 and strains containing the recombinant plasmids were selected on YPD agar with 200 μg/mL geneticin (G418 – Sigma A1720-5G).
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products from each targeted exon were purified and mixed with 0.5 units of recombinant spCas9-GFP (Sigma-Aldrich, Cat # CAS9GPRO), 10 μM gRNA in 2 μL of 10x Cas9 reaction buffer ...
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bioRxiv - Cell Biology 2024Quote: ... The recombinant protein from the cell lysate was purified using Glutathione Sepharose® 4B GST affinity columns (Millpore Sigma, GE17-0756-05). The purified PCM1-GST fusion protein was analyzed on SDS-PAGE stained with Coomassie Blue (Invitrogen) ...
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bioRxiv - Genetics 2021Quote: ... Anti-His (SCBT, #sc-8036; used in 1:2000), anti-GST (SCBT, #459; used in 1:5000) and anti-Flag (Sigma-Aldrich, #F7425;used in 1:2000). Peroxidase conjugated anti-mouse (Jackson Immunoresearch ...
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bioRxiv - Cell Biology 2019Quote: ... Protein eluates were separated by SDS PAGE and stained with Coomassie Brilliant Blue or with anti-His antibody after transfer onto a nitrocellulose membrane (Sigma-Aldrich, Steinheim, Germany; dilution 1: 5000).
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bioRxiv - Cell Biology 2019Quote: ... The proteins of the eluate were separated by SDS PAGE and stained with Coomassie Brilliant Blue or with anti-His antibody after transfer onto nitrocellulose membrane (Sigma-Aldrich, Steinheim, Germany; dilution 1: 5000).
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bioRxiv - Genetics 2021Quote: ... mice were treated with E2 (6.7 ng per mouse) and P4 (P0130, Sigma-Aldrich; 1 mg per mouse) for 3 days ...
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bioRxiv - Cell Biology 2019Quote: ... The primary antibodies were mouse anti-KCa1.1 (1:500; NeuroMab) and mouse anti-α-tubulin (1:10,000; Sigma).
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bioRxiv - Cancer Biology 2020Quote: ... Mouse monoclonal antibodies against human LMα3 (clone CL3112) and mouse IgG (both Sigma-Aldrich, St. Louis, Missouri, USA) were used at 0.5 μg mL−1.
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bioRxiv - Cell Biology 2021Quote: ... along with the appropriate antibody (mouse HA 12CA5, 7.5 μl 0.4 mg/ml, Roche; mouse M2 FLAG, 5 μl 1 mg/ml, Sigma), before incubating at 4 °C on a rotating wheel at 14 rpm for 15-18 h ...
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bioRxiv - Bioengineering 2020Quote: ... The mouse anti-HA antibody (Covance, Princeton, NJ, USA) and goat anti-mouse IgG-alkaline phosphatase (Sigma-Aldrich) were added to the wells for incubation successively ...
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bioRxiv - Cell Biology 2021Quote: ... goat-anti-mouse and goat anti-rabbit Abberior STAR 635P and goat anti-mouse Atto 647N (Sigma-Aldrich), together with Atto 647N Phalloidin (ATTO-TEC ...
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bioRxiv - Neuroscience 2020Quote: ... They were then incubated with primary monoclonal mouse anti-TH antibody (mouse monoclonal MAB5280, Millipore, France, 1/2500) diluted in TBST containing 1% NGS overnight (4°C) ...
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bioRxiv - Microbiology 2021Quote: ... using the mouse sera at dilution 1:1000 and anti-mouse IgG antibody conjugated to peroxidase (Sigma Aldrich), the antibody response specific to the EV-associated proteins was examined ...
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bioRxiv - Developmental Biology 2019Quote: ... mouse anti-Zrf1 (Gfap; IgG1; ZIRC 1:20) and mouse anti-acetylated tubulin (AT; IgG2b; Sigma 1:800). Secondary were all raised in goat and included anti-rabbit conjugated to Alexa 488 (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse monoclonal anti-α-tubulin (T5168, 1:4000) and mouse monoclonal anti-GFP (11814460001,1:2000) from Sigma-Aldrich; rabbit polyclonal anti-Myc (ab9106 ...
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bioRxiv - Microbiology 2020Quote: ... Mouse monoclonal anti-FLAG M2 antibody and Mouse monoclonal anti-β-Actin antibody were purchased from Sigma-Aldrich. Integrin β-1 rabbit polyclonal antibody was purchased from Proteintech (Wuhan ...
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bioRxiv - Cancer Biology 2021Quote: ChIP-grade mouse anti-PLK1 (Cat. # SAB1404220) and mouse IgG (Cat. #sc2025) antibodies were obtained from Sigma Aldrich. Anti-FLAG (Cat ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-vinculin (mouse) and anti-LC3 (mouse) antibodies were purchased from Sigma-Aldrich (St. Louis, MO, USA). Chemiluminescence reagent for Western blot was obtained from BioRad Laboratories (Hercules ...
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bioRxiv - Microbiology 2020Quote: ... and mouse anti-GAPDH (Sigma-Aldrich, G8795). Secondary antibodies IRDye® 680RD goat anti-rabbit IgG and IRDye® 800CW goat anti-mouse IgG (Li-Cor ...
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bioRxiv - Biophysics 2021Quote: ... mouse-anti-HA (1:5,000; Sigma-Aldrich). Secondary antibodies were IRDye680 and IRDye800 antibodies (LI-COR ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti-Flag (Sigma, F1804, 1:1000) and rabbit anti-HA (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti-Flag (Sigma #F1804, 1:1000); mouse anti-Akt (Cell Signaling #2920 ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti-Flag (Sigma #F1804, 1:200); mouse anti-TRIM9 (Abnova #ABN-H00114088-M01 ...
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bioRxiv - Cancer Biology 2021Quote: ... γH2AX (mouse mAb, clone JBW301, Millipore Sigma) histone H3K27me3 (rabbit ...
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bioRxiv - Cancer Biology 2021Quote: ... and mouse-anti GAPDH (MAB374, Millipore Sigma). Secondary antibodies used were HRP anti-rabbit (Rockland ...
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bioRxiv - Biophysics 2020Quote: ... Anti-Mouse IgG (whole molecule)–FITC (Sigma) was added at a 1:100 concentration and placed in the refrigerator for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... and anti-pH2AX (Mouse, Millipore #O5-636) antibodies were diluted at 1:200 in MaxBlock ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-acetylated Tubulin (1/500, Sigma), and chicken anti-GFP (1/1000 ...