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Citations for Millipore Sigma :
2451 - 2500 of 10000+ citations for Mouse IgG2a Isotype Control Antibody Biotin 15H6 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... gondii localized using antibody against the RH strain tachyzoites (67) and ubiquitin was localized using mouse antibody clone FK2 (04-263; EMD Millipore Corporation). Parasites were enumerated per parasitophorous vacuole (PV ...
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bioRxiv - Physiology 2020Quote: ... α-cell/ β-cell ratio was calculated following staining with antibodies against insulin (as above) and glucagon (primary antibody: mouse anti-glucagon 1:2000; Sigma-Aldrich, G2645) (secondary antibody goat anti-mouse Alexa Fluor 488 1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... Immunoprecipitations were performed overnight at 4°C on a rocking wheel using either mouse anti-MAGI1 antibody for endogenous immunoprecipitations or anti-HA antibody and EZview Red anti-FLAG M2 affinity gel (Sigma-Aldrich #F1804) for co-immunoprecipitations ...
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bioRxiv - Microbiology 2022Quote: ... a mouse anti-SARS-CoV-2 nucleocapsid (N) protein primary antibody and an anti-mouse IgG HRP secondary antibody in conjunction with SIGMAFAST™ OPD developing solution (Millipore Sigma) permitted SARS-CoV-2 infection quantification ...
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bioRxiv - Developmental Biology 2021Quote: ... the membrane was then incubated overnight at 4°C with primary antibody 1:12000 mouse anti-α-Tubulin antibody (SIGMA, Cat. # T5168). Followed by 1h incubation with secondary antibody ...
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bioRxiv - Neuroscience 2022Quote: For immunocytochemical staining we used the following primary and secondary antibodies: mouse anti-NFL antibody clone DA2 (Merck Millipore, cat. no. MAB1615), mouse anti-NFL antibody clone F-12 (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2020Quote: Secondary antibodies used are: Goat anti-mouse IgG (H+L) highly cross-adsorbed Alexa fluor plus 647 secondary antibody (Sigma-Aldrich, A32728), Goat anti-mouse IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: ... the membranes were washed with TBST for 3 times and incubated for 1 h with a secondary antibody: anti-mouse IgG antibody (HRD) (Sigma-Aldrich, A9044) and anti-rabbit IgG antibody (HRD ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were transferred to primary antibody solution containing monoclonal mouse anti-vesicular glutamate transporter 1 (VGlut1) antibody (1:750; Millipore, Burlington, MA) in blocking buffer and incubated overnight at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... FLAG-tag immunoprecipitation was performed using an α-FLAG antibody (Monoclonal ANTI-FLAG® M2 antibody produced in mouse, Sigma Aldrich, #F3165) and Protein G magnetic beads (Invitrogen™ Dynabeads™ Protein G ...
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bioRxiv - Genetics 2020Quote: ... using 1:1000 rabbit anti-Bestrophin1 antibody (LAgen Laboratories; Cat# 016-Best1-01) and 1:1000 mouse anti-Actin antibody (Millipore; Cat# MAB1501) as primary antibodies.
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated in PBS-T-milk for 1 h using 1:10000 dilutions of anti-rabbit or anti-mouse HRP-linked secondary antibodies (A0545 or A9044 Sigma antibodies, respectively) as required ...
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bioRxiv - Biophysics 2022Quote: ... Solutions of primary antibodies were prepared in blocking solution using the following dilutions: mouse antibody against SRRM2 (1:2000, Sigma Aldrich, #S4045), mouse antibody against SRSF1 (1:250 ...
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bioRxiv - Neuroscience 2022Quote: ... and 1 hour in secondary antibody (1:500 Alexa Fluor 568 donkey anti mouse antibody, Cat: A10037, Lot: 1917938)) with DAPI (1:1,000 Millipore Sigma, D9542-1MG). Tissue was washed 5 times for 5 minutes each in 1x PBS between each treatment and mounted on Fisherbrand Superfrost Plus slides ...
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bioRxiv - Molecular Biology 2024Quote: ... Yhb1-TAP and α-tubulin proteins were detected using the polyclonal anti-mouse IgG antibody (ICN Biomedicals) and monoclonal anti-α-tubulin antibody (clone B-5-1-2; Sigma-Aldrich), respectively ...
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bioRxiv - Microbiology 2023Quote: ... overnight at 4 °C following incubation with mouse anti-rAgApyrase antibody (1:10,000 in blocking buffer) or rabbit anti-tPA antibodies (1:2500) (Millipore-Sigma, Marlborough, MA, USA). Membranes were incubated with anti-mouse or anti-rabbit horseradish peroxidase (1:2500 in blocking buffer ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted in the Antibody Diluent Reagent from the kit [mouse anti-c-myc (clone 9E10; 1:200, Sigma-Aldrich #11667149001) and rabbit anti-HA (1:200 ...
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bioRxiv - Physiology 2023Quote: ... and incubated in anti-PEMT rabbit polyclonal antibody (1:1000, Bio-techne #NBP1-59580) or anti-α-TUBULIN mouse monoclonal antibody (1:10,000, Millipore-SIGMA #T5168) in 5% dairy milk ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then blocked in 3% BSA before adding primary antibody mouse monoclonal anti-IAV nucleoprotein A1 and A3 blend antibody (EMD Millipore, MAB8251). The primary antibody was followed by a secondary anti-mouse Alexa-488 (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... The membrane was then washed with TBS-T and incubated with 1:10000 goat anti-rabbit or rabbit anti-mouse poly-HRP secondary antibody (Thermo, 32260) and 1:5000 anti-β-actin-peroxidase antibody (Sigma, A3854) in TBS-T for 1 hr ...
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bioRxiv - Microbiology 2022Quote: ... Expression of recombinant antigens in induced and un-induced controls was assessed by immunoblot using a monoclonal anti-poly- histidine antibody (Millipore-Sigma, diluted 1:2000) after SDS-PAGE ...
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bioRxiv - Developmental Biology 2023Quote: ... The signal was compared to antibody stainings of loading controls (H3 and β-Catenin) and the membranes were quality controlled by Ponceau (Sigma Aldrich, #P7170-1L) staining ...
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bioRxiv - Immunology 2020Quote: ... the medium was replaced by medium containing 40 μM of D-biotin (Sigma). The initial time-lapse acquisition characterizing TMEM176B was performed at Institut Curie (Paris ...
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bioRxiv - Plant Biology 2021Quote: ... aestivum (Gerlach & Bedbrook, 1979) was labelled using the Biotin-nick translation mix (Sigma). Digoxigenin-labelled probes were detected with anti-digoxigenin-fluorescein Fab fragments (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... Immunoblotting (IB) at the indicated dilutions: rabbit anti-biotin (IB 1:1000, Sigma), guinea pig anti-MAP2 (IF 1:1000 ...
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bioRxiv - Immunology 2022Quote: ... Free biotin was removed with spin filters with a 10kDa cutoff (Merck, Millipore).
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bioRxiv - Immunology 2022Quote: ... RBCs were biotinylated with controlled concentration of biotin-X-NHS linker (Millipore/Sigma), coated with saturating amount of streptavidin (ThermoFisher) ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated with Dibenzocyclooctyne-PEG4-biotin conjugate (Sigma-Aldrich #760749; 50μM final concentration) in a reaction volume of 100 μl for 1 h on a rotator in slow motion (9 rpm ...
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bioRxiv - Immunology 2022Quote: ... RBCs were biotinylated with controlled concentration of biotin-X-NHS linker (Millipore/Sigma), coated with saturating amount of streptavidin (ThermoFisher) ...
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bioRxiv - Plant Biology 2019Quote: ... α-RFP-biotin was detected using α-rabbit IgG–Alkaline Phosphatase (Sigma, a3687).
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bioRxiv - Biochemistry 2021Quote: hACE2 was chemically biotinylated using the EZ-Link NHS-PEG4-Biotin kit (Sigma). The sample was first desalted using a PD-10 gravity flow column (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... NaN3 (0.02% w/v) and an excess of D-biotin (26.4 mM, Sigma) were added to block residual binding sites ...
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bioRxiv - Immunology 2020Quote: ... The tetramerized peptide–MHC complexes were incubated with 10μM of free biotin (SIGMA) to saturate remaining unbound streptavidins ...
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bioRxiv - Genomics 2020Quote: ... and subsequently hybridized with a biotin-labeled (TTAGGG)3 probe synthesized by Sigma. The North2South® Chemiluminescent Hybridization and Detection Kit (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... as well as the Roche Biotin RNA labeling kit (Sigma Aldrich, Darmstadt, Germany), was used for in vitro transcription (IVT ...
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bioRxiv - Biochemistry 2024Quote: ... IgA deglycosylation was monitored using a biotin–conjugated Helix aspersa agglutinin (HAA) (Sigma) that binds specifically to GalNAc ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were incubated with biotin-conjugated hyaluronic acid binding protein (HABP) (Sigma 385911), followed by streptavidin conjugated to AlexaFluor-488 (ThermoFisher S11223) ...
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bioRxiv - Systems Biology 2023Quote: ... beads were washed twice with 1X PBST + 2 mM biotin (Sigma, #B4639-5G), resuspended in 200 μL of 1x PBST + 2mM biotin ...
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bioRxiv - Biophysics 2023Quote: Mice were injected with 40 μg Tf-biotin conjugates (Sigma-Aldrich, Inc., MO) or PBS buffer and sacrificed 6 hr post-injection ...
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bioRxiv - Biochemistry 2023Quote: Biotinylated RNA duplexes were generated by incorporation of Biotin-16-UTP (Sigma-Aldrich) during in vitro transcription of 1-kb sense and antisense RNA strands (see above for in vitro transcription protocol) ...
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bioRxiv - Cell Biology 2023Quote: ... Release of cargo from the ER was triggered by adding biotin (Sigma-Aldrich) to the live-cell imaging solution at a final concentration of 500 µM.
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bioRxiv - Cancer Biology 2024Quote: ... Biotin switch-treated protein reduced with 2.5 μL of 0.2 M dithiothreitol (Sigma) for 1 hr at 57°C and the samples cooled before adding 2.5 μL of 0.5M iodoacetamide and incubated in the dark for 45 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... one 24 mg/kg intraperitoneal (IP) injection of diluted biotin solution (B4639, Sigma- Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... incubated 15 min in 100 μl 2 μg/ml Atto565-biotin (Sigma-Aldrich), rinsed again in 100 μl PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were treated with either 0.5% DMSO (vehicle control) or 20 µM Dacarbazine (clinical control; Sigma-Aldrich; St. Louis, MO, United States) or HDAC6i (Tubastatin A (SelleckChem ...
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bioRxiv - Immunology 2021Quote: ... Bulk endotoxin-free preparations of VIU-1005 and the empty control plasmid (control) were prepared for animal studies using GenElute™ HP Select Plasmid Gigaprep Kit (Sigma, Germany).
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bioRxiv - Neuroscience 2019Quote: ... 40 μm sections of PFA-fixed mouse brains were stained with the following antibodies: mouse anti-Gad67 (cat. no.: MAB5406; Millipore; 1:1000; RRID: AB_2278725); chicken anti-GFP (cat ...
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bioRxiv - Microbiology 2020Quote: ... Blotting was performed using a mouse monoclonal anti-6x His antibody (1:1000; Millipore HIS.H8) and a goat anti-mouse IgG HRP conjugated secondary antibody (1:2000) ...
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bioRxiv - Biophysics 2021Quote: ... the samples were incubated with the primary antibody (monoclonal mouse anti-vinculin, 1:100, Sigma V9264 ...
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bioRxiv - Biophysics 2019Quote: ... antibodies were used in combination with peroxidase-labeled goat anti-mouse IgG (A4416, Sigma-Aldrich). The reactivity was detected with an Amer-sham ECL Detection Reagent (GE Healthcare Life Science ...