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Citations for Millipore Sigma :
2451 - 2500 of 6152 citations for IL 8 CXCL8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and used for transduction in the presence of 8 µg/mL polybrene (Sigma-Aldrich). 24 hours after transduction the medium was replaced and cells were grown in fresh RPMI medium for 48 hr ...
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bioRxiv - Molecular Biology 2023Quote: ... Viral infections were performed in the presence of 8 μg/mL polybrene (Sigma-Aldrich) for 24 h ...
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bioRxiv - Cancer Biology 2023Quote: ... The M-protein solutions were diluted 1:1 with 8 M Urea (Sigma Aldrich) in Tris.HCl (pH=7 ...
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bioRxiv - Immunology 2023Quote: ... mice were gavaged with 8 mg tamoxifen dissolved in 200 μL corn oil (Sigma). Tamoxifen was dissolved by gentle agitation at 37°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... suspended in rehydration buffer (100 μL) mixed from 8 M urea (Sigma-Aldrich, U6504), thiourea (Sigma-Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... 8-Hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (HPTS) (H1529) were purchased from Sigma-Aldrich. Potassium phosphate monobasic (42420 ...
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bioRxiv - Cancer Biology 2023Quote: 2D cultured cells in 8 wells chamber were fixed with 4% PFA (Sigma-Aldrich) in PBS and permeabilized with 0.5% triton-X 100 in PBS ...
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bioRxiv - Microbiology 2023Quote: ... and resuspending in virus + polybrene (final conc 8 ug/mL, Sigma-Aldrich, TR-1003) or RPMI media + polybrene for the uninfected control at a concentration of 1E6 cells/mL ...
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bioRxiv - Plant Biology 2023Quote: ... Esculin: A 100 μl drop of 8 mg/ml esculin (66778-17-4, Sigma) (Esculin was dissolved in distilled water with 0.2% DMSO and 0.4% absolute ethyl alcohol ...
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bioRxiv - Microbiology 2023Quote: ... Between days 4 and 8 after gametocyte induction 50 mM N-acetylglucosamine (Sigma #A6525) was used to eliminate ABS parasites ...
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bioRxiv - Biochemistry 2023Quote: ... and the pH was adjusted to 8-9 with sodium hydroxide (Sigma-Aldrich, 71687) to decalcify all bones at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... and GM847 cells were transduced in the presence of 8 μg/mL Polybrene (Sigma). For all relevant experiments ...
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bioRxiv - Cell Biology 2022Quote: ... cells after 8 h of meiotic induction were treated with benomyl (Sigma #45339, USA) at a concentration of 120 µg/ml or 0.4% DMSO for 1.5 h before they were analyzed for protein localization.
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bioRxiv - Biophysics 2022Quote: ... (ii) an oxygen-scavenging system (2.5 mM Protocatechuic acid (PCA, pH ∼8, Sigma-Aldrich), 60 nM Protocatechuate-3,4-dioxygenase (PCD ...
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bioRxiv - Developmental Biology 2023Quote: ... and 100 nM 8- Bromoadensoine 3’,5’-cyclic monophosphate sodium salt (Sigma, Cat#B7880) was added ...
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bioRxiv - Developmental Biology 2023Quote: ... medium changed to IMDM+10%FCS+Penicillin-streptomycin + 8-bromo-cAMP (10μM, Sigma-Aldrich), and then incubated overnight at 37°C 5%CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... in PBS and plated in a well of an 8-well EZ Slide (Millipore) which had been pre-coated with 5 ul Matrigel ...
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bioRxiv - Cell Biology 2023Quote: ... with or without E2 (10-8 M; E1024, Sigma-Aldrich, St. Louis, MO, USA) for 3 ...
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bioRxiv - Cell Biology 2023Quote: ... with or without E2 (10-8 M; E1024, Sigma-Aldrich, St. Louis, MO, USA) for 3 ...
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bioRxiv - Genetics 2023Quote: ... in the presence of 8 μg/ml polybrene (AL-118, Sigma-Aldrich, MO, USA). After that time ...
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bioRxiv - Plant Biology 2023Quote: ... resolved on 8% SDS polyacrylamide gels and transferred to PVDF membranes (Immoblon-P, Millipore), which were then blocked with 5% non-fat dry milk for 2 h ...
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bioRxiv - Molecular Biology 2023Quote: ... washed with cold 50 mM EDTA pH 8 and digested with lyticase (Sigma, L4025) in CSE buffer (20 mM citrate/phosphate pH 5.6 ...
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bioRxiv - Microbiology 2023Quote: ... or control BHK21 cells were seeded into 8-well glass chamber slides (Millipore, PEZGS0816) to a final confluency of 80% ...
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bioRxiv - Microbiology 2023Quote: ... or control BHK21 cells were seeded in 8-well glass chamber slides (Millipore, PEZGS0816) to a final confluency of 80% and inoculated with rWA1-D614G or rWA1-Omi-S viruses (MOI = 0.1) ...
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bioRxiv - Cancer Biology 2023Quote: Cell growth and viability assays were both performed using CCK-8 assay (Sigma-Aldrich) per the manufacturer’s directions ...
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bioRxiv - Plant Biology 2023Quote: ... 8 pmol M13-labeled forward primer and 32 pmol reverse primer (Sigma Life Science), standard Taq buffer (New England Biolabs) ...
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bioRxiv - Immunology 2023Quote: ... or the A1 ADO receptor (ADORA1) antagonist 8- cyclopentyl-dipropylxanthine (DPCPX) (10 μM) (Sigma) were added to the medium in the absence of or together with MTX.
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bioRxiv - Cell Biology 2024Quote: ... 6-8 hearts were washed in 20mM butanedione monoxime (BDM)(Sigma-Aldrich, B-0753) in 1x phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2024Quote: ... pH 8) containing protease inhibitors (100 μM PMSF + complete protease-inhibitor cocktail (Sigma P7626) and denatured in Laemmli buffer at 1000C for 5 min ...
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bioRxiv - Biochemistry 2024Quote: IPSC-derived iMNs were cultured in 8-well chamber slides (Millicell EZ slides, Millipore) and fixed with 4% paraformaldehyde (PFA ...
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bioRxiv - Cancer Biology 2024Quote: ... filtered lentiviral supernatant was incubated with cells in 8 µg/ml polybrene (Sigma-Alrich) containing media and spinoculation was performed at 500 xg for 45 min to increase transduction efficiency.
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bioRxiv - Neuroscience 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; human α-syn: 804-258-1001, Enzo Life Science; beta-actin: A4700, Sigma). After incubation with the first antibody ...
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bioRxiv - Immunology 2022Quote: Concentrations of human serum albumin in human plasma and bronchoalveloar lavage fluid were measured using an ELISA kit from Sigma Aldrich (Cat. #RAB0603) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies for immunostaining included rabbit anti-human/mouse PKD-1 (SAB4502371) and mouse anti-human/mouse α-smooth muscle actin (α-SMA, A2547) (Sigma-Aldrich), mouse anti-human/rat CD44 (5640S ...
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bioRxiv - Developmental Biology 2024Quote: ... anti-Ku80 (CST, #2180, 1:400), anti-human nuclear antigen (Novus NBP2-34342, 1:200) and anti-human-specific Albumin (Sigma, A3293, 1:200). Species-specific secondary antibodies conjugated with Alexa Fluor 555 ...
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bioRxiv - Cell Biology 2019Quote: ... the membranes were incubated overnight at 4°C with the adequate primary antibodies in TBST buffer: mouse anti-His-tag (1:2000, Millipore, 05-949), rabbit anti-CAP-D2 serum (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... ICR1-His6 and ICR2-His6 were stained with an anti-His antibody (1:5,000) and secondary antibody conjugated with fluorescein (Sigma; F0257; 1:5,000). The solution was then centrifuged at 12,000 g for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... Cells were routinely passaged in Dulbecco’s modifies Eagle medium (DMEM+) culture media with serum (DMEM, low glucose, from Sigma-Aldrich; 10% HI-FBS; 1% penicillin-streptomycin solution from Sigma-Aldrich; 1% 200mM L-glutamine from Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2021Quote: ... were inserted between the NdeI and XhoI sites of the bacterial expression vector pET-24a containing a HIS-tag (Novagen, Madison, USA). The ligated products were transformed into TOP10 E ...
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bioRxiv - Microbiology 2021Quote: ... from ffmA was PCR amplified and cloned in frame as a NdeI/HindIII fragment upstream of the C-terminal 6x-His tag in pET29a+ (EMD Millipore, Inc.) to form plasmid pSP113 ...
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bioRxiv - Cell Biology 2022Quote: Anti-His tag and microtubule-associated protein 1 light chain 3 beta (LC3) Antibodies were purchased from Millipore Sigma (Burlington, MA, USA). Anti-Adipose Differentiation-Related Protein (ADRP ...
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bioRxiv - Microbiology 2020Quote: Recombinant full length SIC proteins were purified using affinity chromatography with a His-bind column as per manufacturer’s instructions (Novagen, Merck, Darmstadt, Germany). sic1.300 ...
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bioRxiv - Microbiology 2021Quote: N-terminal His-tagged recombinant proteins were expressed in Rosetta™ 2(DE3) pLysS competent cells (71403; Novagen, Inc., Madison, WI, USA) by induction with 0.5 mM isopropyl β-d-1-thiogalactopyranoside at 30°C for 4 h (N ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant containing the nanobody proteins was then purified by immobilized metal-ion affinity chromatography (IMAC) using HIS-Select® Cobalt Affinity Gel (Sigma H8162) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... then concentrated using 15 ml Amicon 100 kDa centrifuge filter columns and purified by nickel column affinity chromatography (Novagen His-Bind Resin) as per the manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: The open reading frame of WsGT4 and WsGT6 was cloned into the NheI/HindIII and NdeI/BamHI sites of pET28a bacterial expression vector in-frame with the C-terminal 6X-His-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a::WsGT4 and pET28a::WsGT6 respectively (Figure S1 A&B) ...
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bioRxiv - Plant Biology 2024Quote: The open reading frame of CfADH1 and CfAKR2b was cloned into the NdeI/EcoRI sites of pET28a bacterial expression vector in-frame with the C-terminal 6X-His-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a::CfADH1 and pET28a::CfAKR2b ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant containing the nanobody proteins was then purified by immobilized metal-ion affinity chromatography (IMAC) using HIS-Select® Cobalt Affinity Gel (Sigma H8162) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... the nitrocellulose membranes containing the purified recombinant fusion proteins were incubated with mouse anti-His antibody [clone HIS.H8] (Millipore Sigma, Burlington, Massachusetts). HRP-conjugated rabbit anti-mouse IgG antibody (SouthernBiotech ...
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bioRxiv - Immunology 2021Quote: ... and all fractions containing IL-22 were concentrated using a 10 kDa centrifugal filter (Merck Millipore, USA).