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Citations for Millipore Sigma :
2451 - 2500 of 3948 citations for IL 23R Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Fluorescent immunostaining for nuclear NFκB and cytoplasmic NFκB was quantified in immortalized C8D30 astrocytes following 2-hr treatment with 1μg/ml LPS or a cocktail of 3 cytokines: Il-1α (3ng/ml, Sigma, Cat# I3901), TNFα (30ng/ml ...
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bioRxiv - Neuroscience 2021Quote: ... the reporter cells were exposed to drugs or DMSO vehicle for one hour before the addition of 3 ng/mL IL-1a (Sigma, I3901), 400 ng/mL C1q (MyBioSource ...
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bioRxiv - Immunology 2020Quote: ... extracellular Cn were removed by washing 2X with PBS and the macrophages were cultured for a further 24 h in fresh growth media containing either IFNγ to maintain the M1 polarization state or 100 ng/mL recombinant interleukin-4 (IL-4; Sigma-Aldrich, St ...
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bioRxiv - Immunology 2022Quote: ... PBMCs cultures not supplemented with IL-2 overnight were stimulated with 25 ng/ml phorbol 12-myristate-13-acetate (PMA) (Sigma-Aldrich) and 1 μg/ml ionomycin (Sigma-Aldrich) ...
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bioRxiv - Genetics 2019Quote: ... LP cells were incubated for 5 h with 20 ng/ml PMA and 1 μg/ml ionomycin in the presence of 10 μg/ml Berfeldin A (Sigma, IL). For FACS analysis ...
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bioRxiv - Cell Biology 2019Quote: Genomic DNA was isolated from the si-NC and si-Dnmt3aos-transfected M(IL-4) macrophage cells and was then treated with bisulfite using the Imprint DNA Modification Kit (Sigma-Aldrich). Bisulfite sequencing and pyrosequencing were conducted by Shanghai Sangon Biotech Corporation ...
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bioRxiv - Microbiology 2021Quote: ... All SEC-FPLC described in this study was performed on a GE Healthcare AKTA PV-908 FPLC system (Chicago, IL) on Superdex S200 10/300 GL (Sigma-Aldrich) and Superose 6 10/300 GL (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... and subsequently transduced twice with viral supernatant within 48 hrs in the presence of 50 IU/mL IL-2 and 6 mg/mL polybrene (Sigma-Aldrich). TCR-transduced T cells were expanded by stimulation with anti-CD3/CD28 Dynabeads (500,000 beads/106 cells ...
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bioRxiv - Bioengineering 2021Quote: A1 reactive astrocytes were generated by culturing the purified astrocytes on PDMS coated tissue culture plates and then treating for 24 h with IL-1α (3 ng/ml, Sigma, I3901), TNF-α (30 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... SRP4895A), N-(p-Amylcinnamoyl)anthranilic acid (ACA, A8486), PP2 (P0042), interleukin-8 (IL-8, CXCL8 SRP3098) were purchased from Sigma-Aldrich (Sigma-Aldrich Company Ltd. ...
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bioRxiv - Immunology 2020Quote: ... the wells were washed with PBS-T and 3,3’,5,5’-tetramethylbenzidine peroxidase substrate (TMB, T0440-IL, Sigma, St Louis, MO, USA) added to the wells ...
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bioRxiv - Immunology 2022Quote: ... All cocultures were maintained in SCGM supplemented with IL-2 (120 IU/ml) and in the presence of 10 µM raltegravir (Sigma-Aldrich) and 0.4 µM nevirapine (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... virus supernatant was aspirated and preactivated PBMCs (0.5-0.8 x 106/well) in TexMACS medium supplemented with 100 IU/ml IL-2 and 6 ng/ml polybrene (Sigma-Aldrich) were added to each well ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs were activated with interleukin-2 (IL-2) 450 UI/mL (Proleukin, Novartis pharma) and polybrene 2 µg/mL (Sigma-Aldrich) during at least 2 h before infection.
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bioRxiv - Neuroscience 2024Quote: The viral particle stock was diluted in sterile saline (0.9% NaCl) (Hospira, Lake Forest, IL) with 0.01% Fast Green (Sigma, St. Louis, MI). A 10-µl Hamilton syringe (Hamilton Company ...
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bioRxiv - Developmental Biology 2020Quote: ... Blocking was carried out in freshly made blocking buffer (5% FCS, Sigma-Aldrich, Gillingham, Dorset, UK; 2% BSA, Sigma-Aldrich, Gillingham, Dorset, UK in PBS). Anti-filaggrin primary antibody staining (mouse monoclonal 15C10 from Leica Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... Fixed brain samples were placed securely one a time in an MR-compatible holder immersed in proton-free susceptibility matching fluid (FluorinertTM FC-70; Sigma-Aldrich, St-Louis, MO, USA). In order to assess volume differences throughout the mouse brain ...
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bioRxiv - Biochemistry 2021Quote: J774A.1 murine macrophage-like cells were grown in tissue culture flasks with hydrophobic surface for suspension cells in DMEM/FCS (DMEM high glucose medium (Sigma-Aldrich, St. Louis, United States) supplemented with 2 mM glutamine and 10% heat-deactivated FCS) ...
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bioRxiv - Microbiology 2021Quote: ... the MDCK monolayers were washed twice to remove FCS using PBS containing 1 μg/mL of trypsin treated with N-tosyl-L-phenylalanyl chloromethyl ketone (TPCK) (Sigma-Aldrich, St. Louis, Mo, USA), inoculated with each sample ...
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bioRxiv - Cancer Biology 2022Quote: ... both in the presence and absence of oestradiol (Complete medium with 10% FCS +/- 17-ß Oestradiol 1x10-8 M (Sigma Aldrich – cat no E-060)) in parallel ...
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bioRxiv - Genetics 2021Quote: ... the brains were removed from the fixative and placed into plastic tubes (internal diameter 1 cm, volume 13 mL) filled with a proton-free susceptibility-matching fluid (Fluorinert® FC-770, Sigma-Aldrich, St. Louis, MO). Images of excised brains were acquired on a 7T BioSpec animal MRI system (Bruker Biospin MRI GmbH ...
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bioRxiv - Genetics 2022Quote: ... the brains were removed from the fixative and placed in plastic tubes (internal diameter 1 cm, volume 13 mL) filled with a proton-free susceptibility-matching fluid (Fluorinert® FC-770, Sigma-Aldrich, St. Louis, MO). Images of excised brains were acquired on a 7T BioSpec animal MRI system (Bruker Biospin MRI GmbH ...
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bioRxiv - Microbiology 2023Quote: ... used in this study were previously cultivated in a rabbit kidney cell lineage (RK-13, ATCC CCL-37) in RPMI medium supplemented with 10% fetal calf serum (FCS-Sigma-Aldrich, St. Louis, MO, USA) and gentamicin at 37 °C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... were assessed using the following ELISA kits per manufacturer protocols: (IgG; Euroimmun - EI2606-9601G, IgA; Euroimmun - EI2606-9601A, IL-6; Invitrogen - 88-7066.22, CRP; Millipore Sigma - RAB0096-1KT). In brief ...
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bioRxiv - Immunology 2021Quote: Degranulation and cytokine production of NK cell subsets were determined after cytokine stimulation o/n with IL-12 (10ng/ml, Sigma Aldrich, USA) and IL-18 (5ng/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... and carbonic anhydrase (29 kD) from a combination of two kits (GE Healthcare, catalogue No. 28-4038-41, Chicago, Illinois, IL, USA and MERCK, Sigma Aldrich, catalogue No ...
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bioRxiv - Neuroscience 2021Quote: ... and phosphorylated-tau 181 (p-Tau) were measured according to manufacturer specifications (Luminex xMAP technology; EMD Millipore, Chicago, IL, USA: HNABTMAG-68K), modified by a 1:10 dilution of CSF ...
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bioRxiv - Microbiology 2021Quote: ... The resulting hybridoma cells were plated onto 96-well plates and cultured in HAT selection medium (Hybridoma-SFM [Life Technologies, Grand Island, CA, USA]; 10% FBS; 1 ng/mL mouse IL-6; 100 μM hypoxanthine [Sigma-Aldrich, St ...
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bioRxiv - Neuroscience 2019Quote: Synaptoneurosome samples and a protein ladder were separated on 4-20% SDS-PAGE gels (Pierce, Rockford, IL) and transferred to polyvinyldenine fluoride (PVDF) membranes (Millipore, Billerica, MA). The blots were blocked in PBS containing 0.05% Triton-x (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... conjugated anti-histidine was added and color developed by addition of and 3,3’,5,5’-tetramethylbenzidine peroxidase substrate (TMB, T0440-IL, Sigma, St. Louis, MO, USA). Plates were read at an OD of 450 nm with a SpectraMax Plus plate reader (Molecular Devices ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration was determined using BCA™ protein assay (Pierce, Rockford, IL, USA) using BSA (Sigma- Aldrich, St. Louis, MO, USA) as a standard ...
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bioRxiv - Immunology 2022Quote: ... BLaER1 cells were trans-differentiated for 5-6 day in medium containing 10 ng/mL IL-3, 10 ng/mL M-CSF (MPI of Biochemistry, Munich) and 100 nM β-estradiol (Sigma-Aldrich) at a concentration of 7 × 104 cells/well in 96 well plates ...
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bioRxiv - Developmental Biology 2024Quote: ... Plasma was stored at −30°C for analysis of leptin and insulin (CrystalChem Inc., Chicago, IL, USA) and triglyceride content (Roche triglyceride reagent, Sigma glycerol standard).
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bioRxiv - Microbiology 2024Quote: ... the maintenance and expansion of T cells was done by adding medium with 10 ng/ml interleukin-2 (IL-2, Stemcell) (10 ng/ml) and for activation Lectin from Phaseolus vulgaris (red kidney bean, PHA) (Sigma, 1µg/ml).
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bioRxiv - Genetics 2023Quote: Tissue lysates in RIPA buffer containing protease and phosphatase inhibitors as above were used for ELISA measurements of IL-1 beta (#RAB0275, Sigma Aldrich, GmbH) and IL-6 (#RAB0309 ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were blocked with bovine serum albumin (0.5%) before labeling with anti-STIM1 monoclonal antibody (1:50 dilution; 30 min; Sigma-Aldrich, Rockford, IL). Cells were then washed before the addition of Alexa Fluor 488-conjugated goat anti-mouse IgG (1 µg/ml dilution ...
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bioRxiv - Microbiology 2020Quote: ... bacteria were incubated with 0.5 µg/mL of chicken anti-human C1qA (Sigma-Aldrich), followed by incubation with PE-conjugated donkey F(ab’)2 anti-chicken (Jackson ImmunoResearch) ...
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bioRxiv - Immunology 2021Quote: ... 0.4µg pCMV-VSV-G and 1.6µg of a lentiviral vector (for human shSUV39H1, Sigma, TRCN0000157251 ...
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bioRxiv - Developmental Biology 2021Quote: ... were soaked overnight with human SDF-1 3.3 μg ml−1 (Sigma-Aldrich, S1577) at 4 °C ...
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bioRxiv - Genomics 2021Quote: ... Human HEK293T embryonic kidney cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2021Quote: Human neutrophils were purified from fresh peripheral blood by Histopaque®-1119 (Millipore Sigma) and subsequent discontinuous Percoll® gradient centrifugation (102) ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated with primary antibodies (mouse anti-human synuclein, Sigma S5566 (clone SYN211) for human alpha-synuclein ...
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bioRxiv - Immunology 2022Quote: Human monocytes from 3 donors were culture in 8-well chamber slides (Millipore-Sigma) in SFM ...
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bioRxiv - Immunology 2022Quote: Human monocytes from 3 donors were culture in 8-well chamber slides (Millipore-Sigma) in SFM ...
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bioRxiv - Immunology 2022Quote: Human monocytes from 3 donors were cultured in 8-well chamber slides (Millipore-Sigma) in SFM ...
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bioRxiv - Immunology 2022Quote: Human monocytes from 3 donors were cultured in 8-well chamber slides (Millipore-Sigma) in SFM ...
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bioRxiv - Biochemistry 2020Quote: ... full length human PCNA (accession no. NM182649) was cloned into pETDuet-1 MCS1 (Novagen) Amp+ to obtain 6× His N-terminally tagged protein ...
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bioRxiv - Developmental Biology 2019Quote: ... S4 are anti human Tcf7l2 (N-20, SCBT) and anti-gamma tubulin (T9026, Sigma) HRP coupled secondary antibodies (1/2,000 ...
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bioRxiv - Genomics 2019Quote: ... RNA was extracted from human islets using Trizol (Ambion, UK or Sigma Aldrich, Canada). To clean remaining media from the islets ...
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bioRxiv - Immunology 2019Quote: ... incubated with alkaline phosphatase-conjugated goat anti-human IgG (1 μg/mL, Sigma-Aldrich) for 1 hr at room temperature ...