Labshake search
Citations for Millipore Sigma :
2451 - 2500 of 8634 citations for BD 3 Rat since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... hepatocytes were isolated after serial perfusion of rat liver by Hanks ‘ balanced salt solution (HBSS, Sigma, H6648) and collagenase CLS type II solution (50 mg/150 mL ...
-
bioRxiv - Molecular Biology 2023Quote: ... anti-rabbit or anti-rat alkaline phosphatase-conjugated secondary antibodies (dilution 1:10,000, Sigma-Aldrich; Taufkirchen, DE) in 3% (w/v ...
-
Caloric restriction prevents inflammation and insulin dysfunction in middle-aged ovariectomized micebioRxiv - Physiology 2023Quote: ... Insulin serum was measured using ELISA (Rat/Mouse Insulin ELISA, #EZRMI-13K, Millipore St. Charles, MO, USA)) ...
-
bioRxiv - Physiology 2022Quote: ... Cells were plated onto glass coverslips coated with collagen (type I rat tail, Sigma; 10 μg/cm2) and maintained in a humidified atmosphere of 5% CO2 and 95% air at 37 °C in Williams’ Medium E supplemented with penicillin ...
-
bioRxiv - Biophysics 2022Quote: ... For E-Cadherin stainings a 1:1000 dilution of Alexa 647-conjugated anti-rat (Sigma-Aldrich SAB4600186) was used ...
-
bioRxiv - Biophysics 2022Quote: ... and then incubated for 1h in BSA-PBST with a goat anti-rat CF-568 (Sigma SAb4600086) secondary antibodies ...
-
bioRxiv - Neuroscience 2023Quote: ... rats were fasted for 18 hours before experimentation and anesthetized with Inactin (Sigma T133; 150mg/kg i.p.). A tracheotomy was performed prior to making a midline abdominal incision to expose the ventral stomach ...
-
Dual RNA-seq identifies proteins and pathways modulated during Clostridioides difficile colonisationbioRxiv - Microbiology 2023Quote: ... the Snapwell inserts were coated with a 1:1 ratio of rat tail collagen (Sigma Aldrich, USA) and ethanol (VWR Chemicals ...
-
bioRxiv - Neuroscience 2023Quote: SD rats (5-week-old males) were anaesthetized with sodium pentobarbital (Sigma, 40 mg/kg, intraperitoneal injection). The brain is quickly removed and cooled in ice-cold modified artificial cerebrospinal fluid (ACSF) ...
-
bioRxiv - Microbiology 2023Quote: ... anti-rabbit or anti-rat alkaline phosphatase-conjugated secondary antibodies (dilution 1:10,000, Sigma-Aldrich; Taufkirchen, DE) in 3% (w/v ...
-
bioRxiv - Immunology 2023Quote: Cell seeding on transwell inserts: 120,000 T84 cells were seeded on rat-collagen (Sigma-Aldrich #C7667-25MG) coated 6.5 mm transwell 3.0 µm Pore Polycarbonate Membrane Inserts (Corning ...
-
bioRxiv - Cell Biology 2023Quote: ... the rats were anesthetized by intraperitoneal injection of 10 mg/kg sodium pentobarbital (Sigma□Aldrich, MO, USA). Next ...
-
bioRxiv - Cell Biology 2023Quote: ... The AP-AKI rat model was created by retrograde injection of sodium taurocholate (Sigma□Aldrich, MO, USA) into the pancreatic duct ...
-
bioRxiv - Developmental Biology 2022Quote: Pregnant rats were intraperitoneally injected with poly I:C (10 mg/kg body weight, P1530-25MG, Sigma-Aldrich) on gd 13.5 ...
-
bioRxiv - Neuroscience 2023Quote: ... male and female rat pups were injected with 5µL of 1% carrageenan (CGN; Sigma, St. Louis MO) dissolved in sterile saline into the intraplantar surface of the right hind paw.
-
bioRxiv - Neuroscience 2023Quote: ... rats underwent three conditioning trials to associate a 0.1% saccharin solution (Sigma Aldrich, Missouri, in tap water) with drug injection ...
-
bioRxiv - Neuroscience 2024Quote: ... Each sample was labeled with 5μg mouse anti-NeuN (RRID:AB_2572267) and 4μg rabbit anti-parvalbumin (Swant PV27; 1:400) and rat anti-somatostatin (Millipore) followed by fluorescent secondary antibodies ...
-
bioRxiv - Neuroscience 2023Quote: Neonatal Wistar rats were administered either vehicle (phosphate buffered saline; PBS) or cisplatin (Sigma-Aldrich; 0.3mg/kg) via intraperitoneal injection delivered on 2 occasions ...
-
bioRxiv - Bioengineering 2023Quote: ... The slides were then incubated in the primary antibodies (rat anti-PECAM-1, clone 390, EMD Millipore; rabbit anti-NG-2 chondroitin sulfate ...
-
bioRxiv - Physiology 2024Quote: ... Rats were then given neuromuscular block with pancuronium bromide (2 mg/kg; Sigma-Aldrich, St. Louis, MO).
-
bioRxiv - Neuroscience 2024Quote: Rats were deeply anesthetized with isoflurane and transcardially perfused with cold phosphate-buffered saline (PBS, Sigma, USA), followed by cold 4% paraformaldehyde (PFA ...
-
bioRxiv - Bioengineering 2021Quote: ... A 1 : 2 mixture of elastase (BD Millipore) and saline was injected into the RCCA while maintaining the aneurys clip secured ...
-
bioRxiv - Microbiology 2021Quote: ... with 2% w/v Noble Agar (BD, Sigma). Prior to use ...
-
bioRxiv - Cell Biology 2022Quote: ... phosphotyrosine (clone 4G10, Sigma or PY20, BD Biosciences); phospho-FAK pY407 (polyclonal ...
-
bioRxiv - Cell Biology 2021Quote: ... anti-human THEMIS (Sigma, HPA031425 and BD, 562587), anti-pY34 THEMIS (Generated by Abclonal ...
-
bioRxiv - Immunology 2022Quote: ... (Southern Biotechnology Associates; eBioscience; Sigma and BD Biosciences). Flow cytometry cell sorting was performed on an ARIA 3 (BD Biosciences ...
-
bioRxiv - Neuroscience 2022Quote: ... and triethyl amine were made in MilliQ ultrapure water (18 megaohm resistance) and 1-octen-3-ol was diluted in paraffin oil (EMD Millipore #PX0045-3). Aliquots of 10 µL of each compound and dilution was pipetted onto a piece of filter paper (5 × 20 mm ...
-
bioRxiv - Molecular Biology 2019Quote: ... Cells were then washed 3 times with PBS and incubated with an anti-active Caspase-3 antibody (EMD Millipore, Billerica, MA, 1:200) for 2h at room temperature ...
-
bioRxiv - Bioengineering 2021Quote: ... they were anaesthetized in 3-amino benzoic acid ethyl ester (Tricaine/ethyl 3-aminobenzoate; Sigma Aldrich; 168 μg·ml−1 in Tris pH 7) and embedded in 1% low melting point agarose (UltraPure Agarose ...
-
bioRxiv - Neuroscience 2020Quote: ... N- (Piperidin-1-yl)-5- (4-iodophenyl)-1- (2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM251, 3 mg/kg; Sigma Aldrich, St. Louis, MO), or vehicle (VEH ...
-
bioRxiv - Genetics 2021Quote: ... Mouse neural progenitor cells were routinely passaged 1:2-1:4 every 3-5 days using Accutase and maintained in NS expansion medium on laminin-coated plates (Sigma, 3 μg/ml). Both mESCs and mNPCs were incubated at 37 °C and 5% CO2 ...
-
bioRxiv - Genetics 2020Quote: ... containing either tunicamycin only (Enzo Life-Sciences; 2-3 μg/mL) or tunicamycin and auxin (IAA, indole-3-acetic acid, Sigma-Aldrich; 1 mM), and seeded with OP50-1 bacteria ...
-
bioRxiv - Microbiology 2022Quote: ... and then transferred to medium differing by the absence of histidine (his) and the presence of 5 mM 3-amino-1,2,4-triazole (3-AT; Sigma-Aldrich, Saint-Louis, MO, USA). Homologous recombination between pPC86 and the PCR product then reconstituted the expression cassette encoding the AD-fused prey protein ...
-
bioRxiv - Neuroscience 2024Quote: ... Tissue was mounted on charged slides and dried prior to ethanol washes (70%, 80%, 95%, 100% Ethanol, 3 minutes each) and washed in a histological clearing agent (Xylene substitute; Sigma Aldrich; 3 minutes), and then cover slipped with DPX mounting agent (Sigma Aldrich).
-
bioRxiv - Neuroscience 2023Quote: ... The excess fluorescent probe was removed through molecular weight and the buffer was exchanged with PBS (pH 7.4) using 3 kDa centrifugal filter units (UFC500396, Amicon® Ultra – 0.5mL, 3 kDa, Sigma-Aldrich®, Dublin, Ireland). Absorbance was measured at 555 and 280 nm for all tagged samples and calculations were performed according to manufacturer’s instructions using an arbitrary extinction coefficient of 100,000 and molecular mass of 100,000 SI unit ...
-
bioRxiv - Biophysics 2022Quote: ... SPR2 DNA encoding residues 649-864 was generated using the polymerase chain reaction method (primers: 5’-GGCAGGACCCATATGGGCAGGAGAGGGTGGGATAATAAAGC-3’ and 5’-GCCGAGCCTGAATTCTTACTTGTCGAACTGTTGGAGATCGATTTC-3’) and individually sub-cloned into pET28 (Millipore Sigma, Burlington, MA) using engineered NdeI and EcoRI restriction endonuclease sites ...
-
bioRxiv - Microbiology 2024Quote: ... The chromatic mutants were mixed in different ratios (1:1, 1:3, 3:1) and the mixtures were fixed using 4% paraformaldehyde (Sigma−Aldrich/Merck, Germany) for 4 h at 4°C.
-
bioRxiv - Cell Biology 2020Quote: ... or cellulose solution in N-hydroxysuccinimide (NHS; Aldrich; 100 mM) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Sigma; 100 mM, 90 min, RT)44 ...
-
bioRxiv - Microbiology 2019Quote: ... 5α-androstan-17β-ol-3-one (=17β-hydroxyandrostan-3-one) and 3aα-H-4α(3’-propanoate)-7aβ-methylhexahydro-1,5-indanedione (HIP) were obtained from Sigma-Aldrich (St. Louis, MO, USA). 5α-androstan-3β,17β-diol (=3β,17β-dihydroxyandrostane ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... peptides (<3 kDa) were separated from high molecular size proteins by a 3 kDa cutoff ultrafiltration column (Amicon®, Merck Millipore Ltd., Cork, Ireland). The crude peptides (<3 kDa ...
-
bioRxiv - Immunology 2021Quote: The livers of freshly-sacrificed mice were perfused retrogradely via the IVC(3) with 3 ml of PBS and then 10 ml of 2% paraformaldehyde (Sigma, catalogue# 30525-89-4) in PBS ...
-
bioRxiv - Immunology 2020Quote: ... injection of 106 hemocytes from late third instar larvae of the lethal(3)malignant blood neoplasm [l(3)mbn1] mutant larvae in Drosophila Ringer’s solution (Sigma-Aldrich, St. Louis, MI, USA). Booster injections were given 4 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The expression of 3 × FLAG-tagged MpRBOHB proteins was confirmed by western blot analysis using an anti-3 × FLAG antibody (Sigma-Aldrich, Cat. No. F1804).
-
bioRxiv - Biophysics 2023Quote: ... The surface of the coverslips were then coated with a solution of 3% (v/v) (3-Aminopropyl) triethoxysilane (APTES, Sigma-Aldrich, St. Louis, MO) in ethanol for 20 min at room temperature ...
-
bioRxiv - Cell Biology 2023Quote: ... strains containing a degron tag were arrested at 30 °C for 3 hours (total) and then treated with 250 μM 3-indole acetic acid (Sigma-Alrich, St. Louis, MO) for 30 minutes before harvesting ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: When the tumor sizes reached to 80 mm3 animals were treated with a PFKFB3 inhibitor 3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one) (3PO) (Sigma-Aldrich, Merck, Overijse, Belgium). The animals received intraperitoneal (i.p. ...
-
bioRxiv - Biophysics 2023Quote: 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) dry powders were purchased from Sigma Aldrich (St. Louis, MO, USA). Different amounts of DOPC and DOTAP were used to tune the surface charge of the employed liposomes ...
-
bioRxiv - Biophysics 2023Quote: ... the quench buffer also included 3 μL of 0.8% GDN and 3 μL of 300 mg/ml aqueous suspension of ZrO2-coated silica (Sigma-Aldrich, reference no. 55261-U). The resulting mixture was incubated on ice for 1 min to remove lipids and solubilize prestin in GDN before being filtered through a cellulose acetate spin cup (Thermo Pierce ...
-
bioRxiv - Microbiology 2019Quote: ... Membranes were probed with rat anti-HA antibodies (1:100 to 1:3,000 dilutions; clone 3F10, Sigma, 11867423001), rabbit anti-TgTom40 [44] (1:2,000 dilution) ...
-
bioRxiv - Microbiology 2019Quote: ... Cells were then blocked with ultra-block solution (10% rat sera, 10% hamster sera, 10% mouse sera (Sigma), and 10 μg/ml of anti-CD16/32 (2.4G2 ...