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Citations for Millipore Sigma :
201 - 250 of 7494 citations for hsa mir 373 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... An enhanced chemiluminescence (ECL) detection kit (Merck Millipore) was used to develop the membranes ...
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bioRxiv - Microbiology 2021Quote: ... the Duolink PLA fluorescence detection kit’s protocol (Sigma) was followed ...
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bioRxiv - Neuroscience 2022Quote: Annexin V-FITC Apoptosis Detection Kit (Sigma-Aldrich) was used for detecting surface PS-exposure ...
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bioRxiv - Biochemistry 2020Quote: Fatty acid and globulin-free HSA was purchased from Sigma-Aldrich (Product # A3782). HSA was dissolved in a buffer containing 50 mM potassium phosphate (pH 7.0 ...
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bioRxiv - Immunology 2022Quote: ... and stimulated with 50 ng/ml DNP-HSA (human serum albumin)(A6661, Sigma). For DN2 and DN3 cell culture ...
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bioRxiv - Neuroscience 2023Quote: ... we were blocking the sections in 1 % human serum albumin (HSA; Sigma-Aldrich) in TBS for 1 h ...
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bioRxiv - Immunology 2024Quote: ... for 30 min followed by stimulation with 0.2 μg/ml HSA-DNP (Sigma) for 30 min ...
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bioRxiv - Cancer Biology 2022Quote: ... RT-PCR products were separated by gel electrophoresis in 1% agarose (Sigma). Gene-Ruler 100 bp DNA ladder (ThermoScientific ...
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bioRxiv - Microbiology 2021Quote: ... Cell lines used in this study were negative for mycoplasma contamination as determined with Lookout Mycoplasma PCR Detection Kit (Millipore Sigma). During the course of this study ...
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bioRxiv - Molecular Biology 2020Quote: ... All cell lines tested negative for mycoplasma infection and were routinely tested afterwards using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich). A549 cells (ATCC CCL-185 ...
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bioRxiv - Microbiology 2019Quote: ... All cell lines tested negative for mycoplasma infection and were routinely tested afterwards using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines including stable clones were routinely evaluated for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (MP0035, Sigma-Aldrich) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2023Quote: ... and fed with StemFlex media (ThemoFisher Scientific) and undergo monthly mycoplasma testing to ensure cultures are mycoplasma-free with the LookOut Mycoplasma PCR detection kit (Millipore Sigma). For the differentiation of iPSC-derived microglia-like cells ...
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bioRxiv - Molecular Biology 2022Quote: ... The cell lines tested negative for mycoplasma contamination using a PCR-based method using the VenorGeM Classic Mycoplasma Detection Kit (Sigma-Aldrich). STR-genotyping was performed to confirm the identity of HeLa-JVM ...
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bioRxiv - Cancer Biology 2023Quote: ... All cells were regularly tested for mycoplasma contamination using the LookOut mycoplasma PCR detection kit (Sigma-Aldrich, St. Louis, MO, CA).
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bioRxiv - Biochemistry 2023Quote: ... HEK293T cells used for proteome-derived peptide library generation were tested every six months for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) according to the manufacturer’s instructions (Fig ...
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bioRxiv - Cell Biology 2023Quote: ... Working stocks were screened to ensure the absence of mycoplasma every 3 months using LookOut Mycoplasma PCR Detection Kit (Cat. No. D9307 Sigma, UK). Cells were cultured in Modified Eagle’s Medium supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2023Quote: ... All the cell lines were regularly tested for mycoplasma contamination using the LookOut Mycoplasma PCR detection kit (Product no. #MP0035 Sigma-Aldrich) and maintained in humidified cell culture incubators at 37°C and 5% CO2.
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines were confirmed to be free of mycoplasma contamination (VenorTMGeM Mycoplasma Detection Kit, PCR-based, Sigma Aldrich Cat #MP0025). Cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Cell Biology 2022Quote: Detection of an interaction between proteins was assessed using the Duolink II Detection Kit (Sigma-Aldrich) according to the manufacturer specifications ...
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bioRxiv - Immunology 2022Quote: ... Primer of miR-31 was ordered from Sigma (MIRAP00090) and RNU6-1 was used as the internal control (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: miR-6883 mimic was purchased from Sigma-Aldrich (HMI2616). This double-stranded RNA molecule mimics endogenous mature miRNA when introduced into cells.
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bioRxiv - Cell Biology 2019Quote: ... Cell lines were routinely tested negative for mycoplasma contamination using Universal Mycoplasma detection kit (ATCC, 30-1012K) or LookOut Mycoplasma Detection Kit (Sigma-Aldrich, MP0035). The INF2-KO U2OS cell line made by CRISPR-Cas9 is described elsewhere (Chakrabarti et al. ...
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bioRxiv - Biochemistry 2019Quote: Accessible amino groups in HSA were detected using fluorescamine (Sigma-Aldrich, St. Louis, USA) as described 54 ...
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bioRxiv - Biophysics 2022Quote: ... De-fatted Human Serum Albumin (DE-HSA) lyophilized powder was purchased from Sigma-Aldrich (product no ...
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bioRxiv - Developmental Biology 2023Quote: ... The digestion was terminated by α-MEM media with 10% HSA (Merck Millipore, 823022). After digestion ...
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bioRxiv - Immunology 2023Quote: ... cells were stimulated as indicated with different concentrations of Ag (DNP-HSA, Sigma, #A6661). The degree of degranulation was determined by quantification of β-hexosaminidase release as described previously (40).
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bioRxiv - Cell Biology 2021Quote: HT29-18N2 cells (obtained from ATCC) (RRID:CVCL_5942) were tested for mycoplasma contamination with the Lookout mycoplasma PCR detection kit (Sigma-Aldrich, St. Louis, MO). Mycoplasma negative HT29-18N2 cells were used for the experiments presented here.
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bioRxiv - Microbiology 2022Quote: ... All cell cultures and chlamydial stocks were routinely tested for Mycoplasma contamination using the Mycoplasma PCR detection kit (Sigma, St. Louis, MO). All chemicals and antibiotics were obtained from Sigma unless otherwise noted.
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bioRxiv - Cell Biology 2021Quote: HT29-18N2 cells (obtained from ATCC) (RRID:CVCL_5942) were tested for mycoplasma contamination with the Lookout mycoplasma PCR detection kit (Sigma-Aldrich, St. Louis, MO). Mycoplasma negative HT29-18N2 cells were used for the experiments presented here.
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bioRxiv - Microbiology 2019Quote: ... All cell cultures and chlamydial stocks were routinely tested for Mycoplasma contamination using the Mycoplasma PCR detection kit (Sigma, St. Louis, MO). For E ...
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bioRxiv - Cancer Biology 2021Quote: ... Aforementioned single clone cultures were STR validated and screened for any mycoplasma contamination using PCR based Myco detection kit (Sigma, MP0035-1KT) before use ...
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bioRxiv - Cell Biology 2020Quote: HT29-18N2 cells (obtained from ATCC) (RRID:CVCL_5942) were tested for mycoplasma contamination with the Lookout mycoplasma PCR detection kit (Sigma-Aldrich, St. Louis, MO). Mycoplasma negative HT29-18N2 cells were used for the experiments presented here.
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bioRxiv - Microbiology 2020Quote: ... All cell cultures and chlamydial stocks were routinely tested for Mycoplasma contamination using the Mycoplasma PCR detection kit (Sigma, St. Louis, MO). Chlamydia trachomatis strain L2/434 Bu was obtained from the laboratory of Dr ...
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bioRxiv - Microbiology 2023Quote: ... All cell lines were tested every two weeks for mycoplasma contamination either by in-house DAPI staining or LookOut® Mycoplasma PCR Detection Kit (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2023Quote: ... a polymerase chain reaction (PCR)-based method was used to screen for mycoplasma once a month employing the LookOut® Mycoplasma PCR Detection Kit (MP0035, Sigma-Aldrich) and JumpStart™ Taq DNA Polymerase (D9307 ...
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bioRxiv - Immunology 2024Quote: ... Cell lines were kept at 37°C in 5% CO2 and routinely checked for mycoplasma contamination via LookOut Mycoplasma PCR detection kit (Sigma, Cat. MP0035).
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bioRxiv - Neuroscience 2021Quote: In Situ Cell Death Detection Kit (11684795910, Sigma-aldrich) was used to evaluate cell death in monolayer cultured neural progenitor cells according to the manufacturer’s instruction ...
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bioRxiv - Bioengineering 2020Quote: The Alkaline Phosphatase Detection Kit Fluorescence (Sigma-Aldrich, UK) was used according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... and visualized using the enhanced chemiluminescence detection kit (Sigma). The following primary antibodies were used in this study ...
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bioRxiv - Developmental Biology 2019Quote: ... using Apoptag Peroxidase In Situ Apoptosis Detection Kit (Millipore). The embryos were fixed in 4% PFA for 2 hours at room temperature and then stored in methanol at −20°C and processed according to the standard protocol ...
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bioRxiv - Biochemistry 2021Quote: ... pIMAGO phosphoprotein detection kit from Sigma (Cat. no. 18419) was used and receptor phosphorylation was detected as per the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... malachite green phosphate detection kit (Sigma, Cat No. MAK307) was used ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were visualized using enhanced chemiluminescent detection kit (Millipore). To detect changes in the protein glycosylation ...
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bioRxiv - Immunology 2020Quote: ... for 1 hour at RT and detection of anti-mouse IgG by the addition of pNPP substrate (Sigma-Aldrich). An arbitrary number of ELISA units were assigned to the reference pool and OD values of each dilution were fitted to a 4-parameter logistic curve using SOFTmax PRO software ...
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bioRxiv - Cancer Biology 2019Quote: ... miR-325 and control miRNA were purchased from Sigma-Aldrich.
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bioRxiv - Biochemistry 2022Quote: ... were authenticated by short tandem repeat (STR) profiling and tested for mycoplasma contamination prior to experiments using the LookOut® Mycoplasma PCR Detection Kit (Sigma, MP0035-1KT). HEK293T (ATCC CRL-3216 ...
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bioRxiv - Microbiology 2023Quote: ... All bacterial and eukaryotic cell stocks were confirmed to be Mycoplasma negative using the LookOut Mycoplasma PCR Detection Kit (Sigma, St. Louis, MO). Molecular biology reagents were purchased from ThermoFisher unless otherwise noted.
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bioRxiv - Molecular Biology 2021Quote: Detection of apoptotic cells was determined by using ApopTag® Fluorescein In Situ Apoptosis Detection Kit (Sigma Aldrich). Paraffin sections were deparaffinized and hydrated as described and then treated with proteinase K (20 μg/mL ...
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bioRxiv - Bioengineering 2021Quote: ... Fluorescein isothiocynate human serum albumin (FITC-HSA) was purchased from Sigma Aldrich (Oakville, ON, Canada). Milrinone was purchased from Selleck Chemicals (Burlington ...