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Citations for Millipore Sigma :
201 - 250 of 10000+ citations for Vertical Electrophoresis Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Protein samples were resolved by electrophoresis on 12% SDS–polyacrylamide gels and electrophoretically transferred to polyvinylidene difluoride (PVDF) membranes (Millipore Corporation) for 20 min at 25 V with the Trans-Blot Turbo (Bio-Rad Laboratories) ...
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bioRxiv - Neuroscience 2020Quote: ... in retinal samples were resolved by 10% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto and electro-blotted to nitro-cellulose membranes (Millipore, USA). The membranes were subsequently blocked with 5% nonfat milk for one hour and incubated overnight at 4 °C with primary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins were separated via sodium dodecyl sulfate–polyacrylamide gel electrophoresis and then transferred to a polyvinylidene difluoride membrane (Millipore, USA) followed by blocking with 5% nonfat milk ...
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bioRxiv - Microbiology 2021Quote: ... A total of 20 µg of protein from each sample was subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to the polyvinylidene difluoride (PVDF) membrane (Merck Millipore, USA). Then ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated by blue native electrophoresis in 3-12% gradient gels (82, 83) using 10mg/ml Ferritin (404 and 880 kDa, Sigma F4503) and BSA (66 and 132kDa ...
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bioRxiv - Molecular Biology 2022Quote: ... The extracted proteins were analyzed using 5–20% gradient sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) electrophoresis and transferred onto Immobilon Transfer Membranes (Millipore, INYC00010) for immunoblotting ...
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bioRxiv - Physiology 2021Quote: ... Equal amounts of protein (5 μg) were subjected to 12.5 % SDS-polyacrylamide gel electrophoresis and blotted onto 0.2 mm PVDF membrane (Millipore, Billerica, MA, USA).
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bioRxiv - Immunology 2020Quote: ... Then the cell extracts mixed with 5 × SDS sample buffer were separated by 12 % SDS-polyacrylamide gel electrophoresis and transferred onto a nitrocellulose membrane (Millipore, USA). Subsequently ...
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bioRxiv - Microbiology 2022Quote: ... Approximately equal amounts of purified histones and parasite histones were separated by 15% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (Millipore, USA). Chemiluminescent detection of bands was carried out by using Super Signal West Femto Maximum Sensitivity Substrate (Thermo Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... The supernatant was kept for proteomics analysis and electrophoresis followed by silver staining after addition of fresh 6x sample buffer (ProteoSilver Silver Stain Kit, Sigma-Aldrich).
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bioRxiv - Bioengineering 2019Quote: The freeze-substitution media consisted of 0.2% (w/v) uranyl acetate (SERVA Electrophoresis GmbH, Heidelberg, Germany) in absolute acetone (diluted from 20% uranyl acetate stock in methanol (Sigma-Aldrich)) ...
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bioRxiv - Cell Biology 2020Quote: ... Thirty micrograms of protein per well were run on 10% SDS-polyacrylamide (sodium dodecyl sulphate polyacrylamide) gel electrophoresis and then transferred to PVDF membranes (Millipore, USA). Blots were blocked in 5% bovine serum albumin at room temperature for 1 hour and incubated with primary antibodies overnight at 4° followed by incubation with corresponding secondary antibodies at room temperature for 1 h ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by electrophoresis on 10% SDS-PAGE gels and electrotransferred onto Immobilon-polyvinylidene difluoride membranes (Millipore Sigma, Burlington, MA). After blocking with 5% non-fat milk ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal amounts of proteins (40 mg) were first loaded on SDS-PAGE gels for electrophoresis and then transferred onto polyvinylidene difluoride (PVDF) membranes (IPFL00010, Millipore, US) for primary antibody incubation ...
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bioRxiv - Cell Biology 2020Quote: ... Equivalent amounts of total protein were loaded into a 12% SDS-PAGE for electrophoresis and then transferred into a PVDF membrane (Millipore, USA). After blocking nonspecific binding with 5% skimmed milk in TBST ...
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bioRxiv - Microbiology 2021Quote: ... The purification of virions was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Coomassie brilliant blue (Sigma-Aldrich) staining.
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bioRxiv - Neuroscience 2020Quote: ... whole cell lysates were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a 0.2μm pore-size nitrocellulose membrane (Millipore-Sigma, Burlington, MA). After transfer ...
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bioRxiv - Cancer Biology 2022Quote: ... 40-100 μg of the total protein were separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred onto polyvinylidene difluoride (PVDF) membrane (Millipore, Germany) by the wet transfer method ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were resolved by 10% SDS-polyacrylamine gel electrophoresis (PAGE) and detected by western blot with polyclonal anti-BES13 and anti-tubulin (Sigma-Aldrich). Dephosphorylated BES1 ...
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bioRxiv - Microbiology 2023Quote: ... Proteins from cell lysates were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to polyvinylidene difluoride membranes (Millipore IPVH00010). For native blots ...
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bioRxiv - Plant Biology 2023Quote: ... Total proteins were separated by 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to polyvinylidene fluoride membranes (Merck Millipore, USA). OsJMT1-HA was detected by immunoblotting with HRP-conjugated anti-HA monoclonal antibodies (1:2,000 ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were subjected to SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto Immobilon-FL polyvinylidene difluoride membranes (Millipore, IPFL00010). The membrane was blocked in blocking buffer (Trisbuffered saline with 0.1% Tween 20 (TBST ...
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bioRxiv - Molecular Biology 2023Quote: ... and loaded in 4–12% NuPAGE Bis-Tris gel (Fisher, 10247002) for subsequent electrophoresis and transference to nitrocellulose membrane (Merck-Sigma, GE10600003). Precipitated RNA was analyzed by autoradiography and FLAG-PTBP protein levels were measured by western blot.
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bioRxiv - Cell Biology 2023Quote: ... the samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride membranes (PVDF, Millipore, USA). The membranes were blocked with 5% low-fat milk for 2 h at room temperature (RT) ...
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bioRxiv - Neuroscience 2023Quote: ... Fifteen micrograms of total protein were resolved by denaturing in 10% and 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and were then transferred overnight to polyvinylidene difluoride (PVDF) membranes (Cat. # IPFL00010, Millipore Sigma). 5% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2023Quote: ... Equal amounts of protein were loaded and separated by electrophoresis on 12% SDS-PAGE gels and subsequently transferred to polyvinylidene fluoride (PVDF; Merck-Millipore, Germany). After blocking with 10% skim milk for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Thirty μg of protein lysates were separated using SDS–polyacrylamide gel electrophoresis and transferred to a PVDF membrane (Immobilion-P, Millipore, IPVH00010). After blocking the membranes in 5% BSA in TBST (1× TBS (Cell Signalling) ...
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bioRxiv - Neuroscience 2023Quote: Proteins from the homogenized hippocampi were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then electroblotted onto the polyvinylidene difluoride (PVDF) membranes (EMD Millipore). Blotted membranes were blocked with 5% skim milk in Tris-buffered saline supplemented with 0.1% Tween-20 (TBST ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were resolved on SDS-polyacrylamide gel electrophoresis (PAGE) and detected by immunoblotting with the following antibodies: APP C-terminal fragment (CTF; Sigma, A8717), human Aβ (6E10 ...
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bioRxiv - Immunology 2024Quote: ... Equal amounts of proteins were separated on sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) on 4–12% acrylamide gel (Yeasen, China) and transferred onto 0.22 μm PVDF membranes (Millipore Corporation, USA). Membranes were incubated with primary antibodies (anti-RIP1 ...
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bioRxiv - Molecular Biology 2023Quote: ... were separated in a 12% denaturing polyacrylamide gel electrophoresis (SDS-PAGE) and electrotransferred to a polyvinylidene difluoride membrane (PVDF) (EMD Millipore) at 100V for 1h ...
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bioRxiv - Cell Biology 2020Quote: ... Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS) polyacrylamide gels and blotted onto polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Darmstadt, Germany). After blocking for 1 h with 5% bovine serum albumin ...
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bioRxiv - Synthetic Biology 2019Quote: Cells were lysed in ice-cold cell lysis buffer and equal amounts of the protein were loaded for electrophoresis on denaturing SDS/PAGE gel and then transferred onto PVDF membranes (Millipore, Billerica, MA). The blots were probed with the primary antibodies overnight at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... protein (30 μg for each sample) was separated using 10% SDS- polyacrylamide gel electrophoresis and subsequently transferred onto polyvinylidene difluoride membranes (Millipore, Darmstadt, Germany). After 1 h of blocking with Western blocking buffer (CWbio ...
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bioRxiv - Cancer Biology 2020Quote: ... the samples were separated by premade 5-20% gradient SDS-polyacrylamide gel electrophoresis (PAGE) (Nakalai or Atto) and transferred to Immobilon-FL Polyvinylidene Difluoride (PVDF) membranes (Millipore, Billerica, MA). After blocking with skim milk (Morinaga ...
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bioRxiv - Pathology 2022Quote: ... Twenty micrograms of protein per lane were loaded onto an 8-12% polyacrylamide Gel and then transferred to PVDF membranes using an electrophoresis chamber (Millipore, Shanghai, China) for 1.5 h ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of protein from each sample were loaded into 10-20% Tricine sodium dodecyl sulphate– polyacrylamide gel electrophoresis (SDS-PAGE; Sigma-Aldrich, Sweden), followed by immunoblotting on polyvinylidene difluoride (PVDF ...
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bioRxiv - Bioengineering 2022Quote: ... and subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (10%) and then transferred onto poly vinylidene fluoride membrane (EMD Millipore, MA, USA). The membranes were blocked in phosphate-buffered saline containing 5% fat-free milk for 2 h ...
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bioRxiv - Microbiology 2020Quote: ... and HA-tagged proteins of samples were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and were transferred to a PVDF membrane (Merck Millipore, Tokyo, Japan). The membrane was incubated with rabbit anti-N antiserum or mouse anti-GFP antibody ...
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bioRxiv - Molecular Biology 2019Quote: ... Gel electrophoresis was performed in a Min Gel Tank Life Technology system in 1x Tris Tricine buffer (T1165-500ML Sigma Aldrich Co) at 150 V samples were left to migrate until the protein ladder (PageRuler Plus pre-stained protein ladder 10–250 kDA ...
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bioRxiv - Systems Biology 2020Quote: ... separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a polyvinylidene difluoride membrane (PVDF, Millipore, Bedford, MA, USA). The PVDF membrane was blocked with 5% milk in Tris-buffered saline with Tween-20 (TBST ...
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bioRxiv - Pathology 2021Quote: ... 20 µg of total protein was separated by electrophoresis in a 10% SDS polyacrylamide gel and transferred to a PVDF membrane (Millipore, Billerica, MA) for western blot analysis.
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes (Immobilon-P transfer membrane, EMD Millipore corporation). After blocking the membranes with Blocking One (Nacalai Tesque ...
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bioRxiv - Cell Biology 2019Quote: ... Eluted samples were run on 12% SDS-polyacrylamide gel electrophoresis and probed for receptor using HRP-coupled anti-FLAG M2 antibody (Sigma, 1:2000). Fabs were visualized on gel using Coomassie staining.
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bioRxiv - Cell Biology 2019Quote: ... Eluted samples were run on 12% SDS-polyacrylamide gel electrophoresis and probed for receptor using HRP-coupled anti-FLAG M2 antibody (Sigma, 1:2000). Fabs were visualized on gel using Coomassie staining ...
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bioRxiv - Biochemistry 2021Quote: ... were heat-denatured in loading buffer and separated via 10% gel electrophoresis (SDS-PAGE) followed by transferred onto PVDF membrane (Millipore, Germany, IPVH00010). Then the membrane was blocked with 5% skim milk-TBST solution (20 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Cell Biology 2021Quote: ... 8% SDS-PAGE was used for protein separation by electrophoresis and transferred to a PVDF membrane (Immobilon®-P, Millipore Corporation, IPVH00010). 10% BSA was used to block the membrane followed by protein detection using primary antibody of 1:1000 dilution such as anti-β actin (C4 ...
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bioRxiv - Neuroscience 2021Quote: ... were separated by 7,5% or 10% SDS-polyacrylamide gel (SDS-PAGE) electrophoresis and transferred to polyvinylidene fluoride (PVDF) membranes (EMD Millipore Corporation, USA). Western blot analysis was performed with the following primary antibodies ...
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bioRxiv - Microbiology 2022Quote: ... The amplified PCR products were visualized by 1.5% agarose gel electrophoresis and purified using a Millipore filter plate MSNU030 (Millipore SAS, Molsheim, France). The purified PCR products were then subjected to Sanger sequencing using the BigDye Terminator v3.1 sequencing kit and a 3730xl automated sequencer (Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: Proteins were separated by 8.5% Sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis and transferred onto a polyvinylidene fluoride membrane (Millipore, Immobilon-P, IPVH00010) using a semi-dry transfer unit (PEQLAB) ...