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Citations for Millipore Sigma :
201 - 250 of 10000+ citations for SpectraDye Antibody Labeling Kit IR700 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... freshly purified tau was first reduced with 1 mM DTT for 10 minutes and buffer exchanged into a labeling buffer (20 mM Tris pH 7.4, 50 mM NaCl, and 6 M guanidine HCl) using Amicon concentrators (Millipore) (17) ...
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bioRxiv - Biochemistry 2022Quote: ... protein destined for dye labeling was concentrated in an Amicon Ultra-15 centrifugal filter units (30 kDA, Millipore #UFC9030). These samples were then mixed at a molar ratio of 1:1 with Cyanine3 NHS-Ester (Lumiprobe #21020 ...
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bioRxiv - Microbiology 2020Quote: ... antibodies and visualized using Immobilon Western Chemiluminescent HRP substrate kit (Millipore®) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2020Quote: ... We adapted the FlowCellect Autophagy LC3 antibody-based assay kit (FCCH100171, Millipore) as follows ...
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bioRxiv - Genetics 2023Quote: ... using the Montage antibody purification kit according to manufacturer’s instructions (Millipore Sigma), and dialyzed against PBS containing 10% glycerol and aliquots stored at -80°C.
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bioRxiv - Microbiology 2021Quote: Isotyping of the monoclonal antibodies and polyclonal sera responses was performed using the Mouse Monoclonal Antibody Isotyping Kit (Sigma-Aldrich), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Labeling of the DNA with biotin was confirmed in dot blots using HRP-conjugated streptavidin (Sigma S5512, 1 µg/ml) for detection ...
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bioRxiv - Genomics 2019Quote: ... The reaction was carried out at 60°C for 2 hours in a custom labeling buffer with a final concentration of 50 mM K-acetate (Sigma), 10 mM Mg-Acetate (Sigma) ...
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bioRxiv - Biochemistry 2019Quote: ... were seeded on coverslips and incubated at their indicated oxygen concentrations for 24 h prior to treatment with 10 μmol/l bromodeoxyuridine (BrdU) cell proliferation labeling reagent (Sigma) for 1 h ...
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bioRxiv - Bioengineering 2020Quote: ... Ten µl of the C18 SPE purified extracts were DMB-labeled with 60 µl of DMB labeling reagent (Sigma-Aldrich), at 50°C for 2.5 h in the dark under shaking conditions at 750 rpm (Hara et al. ...
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bioRxiv - Cell Biology 2022Quote: ... The standard labeling conditions involved incubation of cells at 37°C for 120 min with 500 µM biotin (Sigma, B4501), diluted from a 100 mM biotin stock solution made in DMSO ...
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bioRxiv - Developmental Biology 2019Quote: 2-[4-(Dimethylamino)styryl]-1-ethylpyridinium iodide (DASPEI) labeling in live fish was performed by diluting 1mM DASPEI (Sigma-Aldrich) in system water ...
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bioRxiv - Plant Biology 2020Quote: 15N-accumulation assay after 15N-nitrate labeling was performed with 15N-labeled KNO3 (99 atom % 15N, Sigma-Aldrich, no. 335134). For 15N-nitrate accumulation assay ...
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bioRxiv - Immunology 2022Quote: ... FITC labeling was accomplished by incubating bacteria (5 × 107 CFU in 100 μl PBS) with 20 µg FITC (Sigma-Aldrich) at room temperature for 30 min as described (73) ...
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bioRxiv - Neuroscience 2023Quote: In order to visualize the BDA labeling, the remaining sections were processed using the avidin-biotin-peroxidase method (Vector, US) with 3,3’-diaminobenzidine (DAB; Sigma, USA) as the chromogen ...
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bioRxiv - Cell Biology 2022Quote: ... using the anti-DNP primary antibody provided with Oxiblot assay kit (Millipore, S7150).
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bioRxiv - Cell Biology 2020Quote: ... to a final concentration of 1mM for 1 minute after which the labeling reaction was stopped by removing the medium and washing once with quenching buffer (5 mM Trolox (Sigma-Aldrich) and 10 mM sodium ascorbate (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... 0 dpf embryos were transferred to 10 cm petri dishes containing 40 ml of labeling media either with or without 2 mM H2O2 (Sigma-Aldrich) at a density of 1000ul of embryo medium/embryo and kept at 28°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 pmol of DNA probe was used for 5′ terminal γ-32P labeling followed by cleanup with ProbeQuant G-50 columns (Sigma) when assessing mRNA levels ...
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bioRxiv - Molecular Biology 2020Quote: ... The labeling solution was aspirated and cells were washed twice with 25 mL of quencher solution (10 mM sodium azide [Sigma Aldrich, Cat.# S2002-5G] ...
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bioRxiv - Immunology 2020Quote: ... The bioswitch labeling procedure was performed according to the protocols described in (11, 45) with modifications using N-ethylmaleimide (NEM, Sigma Aldrich) to alkylate the reduced thiols ...
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bioRxiv - Cancer Biology 2020Quote: The sections from two biological replicate experiments were stained using a Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay (Millipore S7165), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... with the control and labeling diets containing regular L-methionine (Met) and methyl-13C-L-methionine (13C-Met, 99%, Sigma-Aldrich), respectively (i.e. ...
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bioRxiv - Cell Biology 2022Quote: ... were anesthetized with isoflurane and underwent retro-orbital injection with 100 μl PKH26 Red Fluorescent Cell Linker Dye for Phagocytic Cell Labeling (catalog number PKH26PCL-1KT; Millipore Sigma) 1 day before lung challenge ...
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bioRxiv - Biochemistry 2022Quote: ... Valine only labeling used 150 mg/L 2-Keto-3-(methyl-d3)-butyric acid-4-13C,3-d sodium salt (Sigma 691887) and 150 mg/L sodium 4-methyl-2-oxovalerate (Sigma K0629) ...
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bioRxiv - Biochemistry 2022Quote: ... 13C-ILV labeling used 150 mg/L 2-Keto-3-(methyl-d3)-butyric acid-4-13C,3-d sodium salt (Sigma 691887) and 70 mg/L 2-Ketobutryic acid-4-13C,3,3,d2 sodium salt hydrate (Sigma 589276 ...
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bioRxiv - Immunology 2022Quote: ... Approximately 1 million effector cells and target cells were both spun down at 400 × g for 5 min followed by labeling with 1 μM PKH67 and PKH26 fluorescent dyes (Sigma-Aldrich) respectively according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... Anti-sense DIG-labeled in-situ probes were generated using linearized plasmid cDNA templates with 10X DIG RNA labeling mix (Sigma 11277073910) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Anti-sense DIG labeled in- situ probes were generated using linearized plasmid cDNA templates with the10X DIG RNA labeling mix (Sigma 11277073910) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... 600 μg protein equivalents of exosomes pelleted by ultracentrifugation were resuspended in 500 μl Diluent-C for membrane labeling (Sigma CGLDIL) and then mixed 1:1 with 500 μl Diluent-C containing 2 μl CellVue Claret Far-Red Fluorescent Membrane Linker (Sigma MINCLARET) ...
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bioRxiv - Developmental Biology 2023Quote: ... Full lengthrfx6.L and pdx1.L anti-sense DIG-labeled in-situ probes were generated using linearized plasmid cDNA templates with 10× DIG RNA labeling mix (Sigma 11277073910) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2024Quote: ... The labeling solution contained the following reagents at the stated final concentrations in PBS: 2 mM Copper(II) Sulfate Pentahydrate (Sigma 12849), 8 µM Sulfo-Cyanine5 Azide (Lumiprobe B1330) ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted in the Antibody Diluent Reagent from the kit [mouse anti-c-myc (clone 9E10; 1:200, Sigma-Aldrich #11667149001) and rabbit anti-HA (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... Antigen-antibody complexes were visualized by enhanced chemiluminescence using Immobilon Western blotting kit (Millipore).
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bioRxiv - Cell Biology 2020Quote: ... Normal mouse/rabbit IgG or RNA Polymerase II antibodies (provided by Millipore ChIP kit) were used as negative or positive control ...
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bioRxiv - Microbiology 2019Quote: ... The antigen-antibody complexes were visualized by the Immubilon™ chemiluminescent detection kit (Millipore).
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bioRxiv - Cancer Biology 2023Quote: ... and bound antibodies were detected using an ECL detection kit (Immobilon Western ECL, Millipore) or with the ChemiDoc Imaging Systems (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: ... secondary antibodies were added and the proteins were detected using an ECL kit (Millipore). Immunoreactive signals were visualized using the GE Amersham Imager 600 chemiluminescence system.
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bioRxiv - Biophysics 2023Quote: For all remaining experimental steps and sample processing and labeling (Fixation, permeabilization, and immunostaining, etc) materials used included: paraformaldehyde (Sigma-Aldrich, P6148), Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Cre-induction for the lineage tracing or total CAM labeling experiments was induced with a single intraperitoneal injection of Tamoxifen (Sigma- Aldrich; T5648) (100µg or 4mg in corn oil ...
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bioRxiv - Microbiology 2024Quote: A standard curve for Alcian Blue labeling of acid polysaccharides was prepared with Xanthan Gum (“XG”; Sigma-Aldrich G1253, ordered 10/2022) using the updated method of Bittar et al ...
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bioRxiv - Cell Biology 2022Quote: ... Detection of the hybridized antibody was performed using an ECL detection kit (Immobilon Western, Millipore), and the signal was visualized with a digital camera (ChemiDoc XRS+ ...
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bioRxiv - Physiology 2023Quote: ... Antibody binding was detected using an enhanced chemiluminescence HRP substrate detection kit (Millipore; Watford, UK). The following antibodies were used ...
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bioRxiv - Physiology 2023Quote: ... IELs were stained with the following antibodies: Live/DeadTM Fixable Dead Stain kit (Sigma; L23105), CD45 (V500 ...
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bioRxiv - Plant Biology 2020Quote: 15N-accumulation assay after 15N-nitrate or 15N-ammonium labeling was performed with 15N-labeled KNO3 (99 atom % 15N, Sigma-Aldrich, no. 335134) or 15N-labeled NH4Cl (98 atom % 15N ...
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bioRxiv - Microbiology 2023Quote: Lipid II-Atto565 was obtained by labeling of lipid II with a 10-fold excess of Atto565-NHS ester (Sigma-Aldrich, Taufkirchen, Germany) in presence of 0.6 vol% diisopropylethylamine (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... the supernatant discarded and the cells re-suspended in 500 µl of azide labeling solution (1X PBS, 1 mM CuSO4 (Sigma-Aldrich, C1297-100G), 100 µM Fluorescein Azide (Sigma-Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... Acetamide-labeling was achieved by addition of a 4.5-fold molar excess of iodoacetamide (Sigma-Aldrich; 50 mM stock in 100% d6-DMSO) and the labeling reaction was incubated ON at 4 °C in the dark ...
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bioRxiv - Neuroscience 2021Quote: ... The isotype of RNF5 was determined using the IsoStrip Mouse Monoclonal Antibody Isotyping Kit (Sigma-Aldrich) and found to be IgG2b with a kappa light chain.
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bioRxiv - Bioengineering 2019Quote: ... The isotype of lead clone was determined using mouse antibody isotyping kit (Sigma, St. Louis, MO).