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Citations for Millipore Sigma :
201 - 250 of 10000+ citations for SpectraDye Antibody Labeling Kit 550 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... we synthesized DIG-labeled RNA probes with the DIG RNA labeling mix (Sigma-Aldrich; Cat. No. 11277073910) and T7 RNA polymerase (Promega ...
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bioRxiv - Biochemistry 2019Quote: ... 12CH2O formaldehyde was used for labeling of WT proteome and heavy 13CD2O formaldehyde (Sigma, cat. no. 596388) for the VPE0 quadruple mutant proteome ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell death was assessed by Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL; S7100, Merck Millipore). Images were obtained with a Leica DM3000 microscope with a mounted Leica DFC420 camera ...
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bioRxiv - Cell Biology 2023Quote: ... Proximity labeling was induced overnight by supplementing the medium with 10 μM Biotin (Sigma-Aldrich, cat. #B4501). The cells were lysed ...
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Activation of glucocorticoid receptor signaling inhibits KSHV-induced inflammation and tumorigenesisbioRxiv - Cancer Biology 2023Quote: ... Cell cycle was examined by flow cytometry following 5′-bromo-2′-deoxyuridine (BrdU) labeling (BrdU: Sigma-Aldrich, MB5002 ...
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bioRxiv - Developmental Biology 2023Quote: ... Working labeling solution was a 1:10 dilution of the stock solution in 0.3 M sucrose (Sigma). Embryos were injected with labeling solution in the ventral tailbud by mouth pipetting using a glass capillary and collected individually in a 24 well-plate on ice ...
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bioRxiv - Evolutionary Biology 2019Quote: The CcP activity was measured by monitoring at 550 nm the oxidation of Cytochrome c (from the bovine heart, Sigma-Aldrich) previously reduced ...
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bioRxiv - Bioengineering 2020Quote: ... After that, the sample of benzalkonium chloride was prepared by dissolving 10 mg in 550 μL D2O (D, 99.9 %) (Sigma-Aldrich, Norway) and transferred to 5mm LabScape Stream NMR tube ...
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bioRxiv - Bioengineering 2022Quote: ... samples were incubated for 1 h with 1 μg/ml DAPI and 50 pmol Atto 550-conjugated Phalloidin (19083, Sigma-Aldrich). Samples were washed and imaged in PBS images were acquired with a confocal laser scanning microscope (Leica TCS SP8X ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were imaged (Ex/Em – 561 and 650 nm for MtPhagy dye and 488/550 nm for Lysosomal dye) using an Amnis ImagestreamX MKII imaging flow cytometer (EMD Millipore). Appropriate unstained cell and single-color cell controls were used to generate a compensation matrix which was used to convert raw image files to compensated image files and the co-localization wizard from IDEAS® v6.0 (Amnis ...
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bioRxiv - Microbiology 2021Quote: ... antibodies and visualized using Immobilon Western Chemiluminescent HRP substrate kit (Millipore) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2022Quote: Antibodies against Glut1 and TRAP staining kits were ordered from Sigma. Antibodies against MMP13 ...
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bioRxiv - Microbiology 2023Quote: ... the H-Tag Antibody HRP Conjugate Kit (Merck-Millipore, Darmstadt, Germany) was used according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2019Quote: ... F-actin labeling was done using Alexa Fluor 488-conjugated phalloidin (2 u/ml 66 nM, Sigma-Aldrich) and nucleus staining was done with DAPI (1:1000 ...
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bioRxiv - Biochemistry 2019Quote: For fluorescent labeling of Ag85B the green fluorescent dye CFTM 488A succinimidyl ester (Sigma-Aldrich, St. Louis, USA) was used ...
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bioRxiv - Cancer Biology 2021Quote: ... Chemical labeling of live cells was achieved by adding 10 μl of hydroxylamine solution (Sigma: 467804, ~15M solution) and incubated for 10 minutes with gently vortexing intermittently ...
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bioRxiv - Neuroscience 2021Quote: ... including an additional labeling against acetylated-α-tubuline (monoclonal, 1:1000 in blocking solution; Sigma cat No. T6793), which was detected with Alexa488 conjugated goat anti-mouse (1:250 in blocking solution ...
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bioRxiv - Plant Biology 2023Quote: Isolation of Gluconacetobacter xylinus (acetobacter) cellulose and quantum dot (Qdot) labeling of Cel7A (Sigma-Aldrich; Cas: E6412-100UN) were carried out as previously reported (29) ...
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bioRxiv - Molecular Biology 2023Quote: ... DMSO-treated controls were treated 2 hours prior to EdU labeling with 10 μl DMSO/plate (Sigma, D8418). Inhibitors and DMSO were included for the duration of EdU labeling and chromatin maturation in all experiments.
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bioRxiv - Biophysics 2024Quote: ... All other chemicals and reagents for labeling and fluorescence measurements were purchased from Sigma-Aldrich (St. Louis, MO). 2 M hydroxylamine hydrochloride in water was prepared for use in Fe2+ experiments and used for only one day ...
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bioRxiv - Cell Biology 2023Quote: ... For fluorescent imaging the 30 µM protein solutions were supplemented with 300 nM (100:1 molar ratio) NTA-Atto 550 (Sigma Aldrich, Merck). Movies were acquired at 37°C with a PerkinElmer UltraVIEW VoX microscope equipped with Yokogawa CSU-X1 spinning disk head and Nikon TiE microscope body with an Apo 60x/1.49 numerical aperture (NA ...
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bioRxiv - Molecular Biology 2021Quote: ... 1/1000) antibodies for 2 h at 37 °C in antibody diluent solution (Duolink in situ kit, Sigma-Aldrich). The cells were then labeled with the PLA antimouse PLUS probe (DUO92001 ...
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Tracking of quiescence in Leishmania by quantifying the expression of GFP in the ribosomal DNA locusbioRxiv - Microbiology 2019Quote: To test BrdU labeling in promastigotes and amastigotes the cells were cultured in complete M199 media containing 0.05 mM BrdU (Sigma) for 4 ...
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bioRxiv - Biophysics 2020Quote: ... The isotope labeling reactions were quenched by acidification (pDcorrected 2.5) with pre-chilled quenching solution containing urea (Urea-d4; Sigma) to a final urea concentration of 1.52 M (0.73 μM protein) ...
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bioRxiv - Biochemistry 2022Quote: The samples for total cysteine content labeling were reduced with 5mM Tris (2-carboxyethyl) phosphine (TCEP) (646547 Sigma-Aldrich). Total cysteine content was labeled with tandem mass tag (TMT ...
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bioRxiv - Biochemistry 2019Quote: ... This 2x denaturing/labeling buffer contained 50 mg/mL (∼10 mM) methoxypolyethylene glycol 5000 maleimide (PEG-Mal, Sigma-Aldrich) dissolved in the 2x denaturing buffer described above ...
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bioRxiv - Biophysics 2019Quote: ... freshly purified tau was first reduced with 1 mM DTT for 10 minutes and buffer exchanged into a labeling buffer (20 mM Tris pH 7.4, 50 mM NaCl, and 6 M guanidine HCl) using Amicon concentrators (Millipore) (17) ...
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bioRxiv - Biochemistry 2022Quote: ... protein destined for dye labeling was concentrated in an Amicon Ultra-15 centrifugal filter units (30 kDA, Millipore #UFC9030). These samples were then mixed at a molar ratio of 1:1 with Cyanine3 NHS-Ester (Lumiprobe #21020 ...
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bioRxiv - Microbiology 2020Quote: ... antibodies and visualized using Immobilon Western Chemiluminescent HRP substrate kit (Millipore®) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2020Quote: ... We adapted the FlowCellect Autophagy LC3 antibody-based assay kit (FCCH100171, Millipore) as follows ...
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bioRxiv - Genetics 2023Quote: ... using the Montage antibody purification kit according to manufacturer’s instructions (Millipore Sigma), and dialyzed against PBS containing 10% glycerol and aliquots stored at -80°C.
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bioRxiv - Neuroscience 2021Quote: ... after passing through a dichroic mirror (Di01-R405/488/561/635-25×36; Semrock, Rochester, NY, USA) and an emission filter (YIF-BA510-550S; SIGMA KOKI, Tokyo, Japan). To acquire an OT-dependent signal and an isosbestic signal ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 μM mVP40 proteins or His-tag alone were incubated with 50 μg/ml Ni-NTA-Atto 550 dye (Millipore Sigma, Burlington, MA) in a final volume of 500 μl ...
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bioRxiv - Microbiology 2023Quote: ... WR99210-resistant parasites appeared at ∼21 days post-transfection and the parasites were treated with medium supplemented with 550 μg/ml neomycin (G418 disulfate salt; Sigma-Aldrich; Taufkirchen, DE) for two weeks to remove WT NF54 parasites from the culture ...
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bioRxiv - Microbiology 2021Quote: Isotyping of the monoclonal antibodies and polyclonal sera responses was performed using the Mouse Monoclonal Antibody Isotyping Kit (Sigma-Aldrich), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Labeling of the DNA with biotin was confirmed in dot blots using HRP-conjugated streptavidin (Sigma S5512, 1 µg/ml) for detection ...
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bioRxiv - Genomics 2019Quote: ... The reaction was carried out at 60°C for 2 hours in a custom labeling buffer with a final concentration of 50 mM K-acetate (Sigma), 10 mM Mg-Acetate (Sigma) ...
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bioRxiv - Biochemistry 2019Quote: ... were seeded on coverslips and incubated at their indicated oxygen concentrations for 24 h prior to treatment with 10 μmol/l bromodeoxyuridine (BrdU) cell proliferation labeling reagent (Sigma) for 1 h ...
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bioRxiv - Bioengineering 2020Quote: ... Ten µl of the C18 SPE purified extracts were DMB-labeled with 60 µl of DMB labeling reagent (Sigma-Aldrich), at 50°C for 2.5 h in the dark under shaking conditions at 750 rpm (Hara et al. ...
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bioRxiv - Cell Biology 2022Quote: ... The standard labeling conditions involved incubation of cells at 37°C for 120 min with 500 µM biotin (Sigma, B4501), diluted from a 100 mM biotin stock solution made in DMSO ...
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bioRxiv - Developmental Biology 2019Quote: 2-[4-(Dimethylamino)styryl]-1-ethylpyridinium iodide (DASPEI) labeling in live fish was performed by diluting 1mM DASPEI (Sigma-Aldrich) in system water ...
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bioRxiv - Plant Biology 2020Quote: 15N-accumulation assay after 15N-nitrate labeling was performed with 15N-labeled KNO3 (99 atom % 15N, Sigma-Aldrich, no. 335134). For 15N-nitrate accumulation assay ...
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bioRxiv - Immunology 2022Quote: ... FITC labeling was accomplished by incubating bacteria (5 × 107 CFU in 100 μl PBS) with 20 µg FITC (Sigma-Aldrich) at room temperature for 30 min as described (73) ...
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bioRxiv - Neuroscience 2023Quote: In order to visualize the BDA labeling, the remaining sections were processed using the avidin-biotin-peroxidase method (Vector, US) with 3,3’-diaminobenzidine (DAB; Sigma, USA) as the chromogen ...
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bioRxiv - Cell Biology 2022Quote: ... using the anti-DNP primary antibody provided with Oxiblot assay kit (Millipore, S7150).
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bioRxiv - Cell Biology 2020Quote: ... to a final concentration of 1mM for 1 minute after which the labeling reaction was stopped by removing the medium and washing once with quenching buffer (5 mM Trolox (Sigma-Aldrich) and 10 mM sodium ascorbate (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... 0 dpf embryos were transferred to 10 cm petri dishes containing 40 ml of labeling media either with or without 2 mM H2O2 (Sigma-Aldrich) at a density of 1000ul of embryo medium/embryo and kept at 28°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 pmol of DNA probe was used for 5′ terminal γ-32P labeling followed by cleanup with ProbeQuant G-50 columns (Sigma) when assessing mRNA levels ...
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bioRxiv - Molecular Biology 2020Quote: ... The labeling solution was aspirated and cells were washed twice with 25 mL of quencher solution (10 mM sodium azide [Sigma Aldrich, Cat.# S2002-5G] ...
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bioRxiv - Immunology 2020Quote: ... The bioswitch labeling procedure was performed according to the protocols described in (11, 45) with modifications using N-ethylmaleimide (NEM, Sigma Aldrich) to alkylate the reduced thiols ...