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Citations for Millipore Sigma :
201 - 250 of 4323 citations for Recombinant Human CA10 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... PANC0813 medium was supplemented with 10units/mL human recombinant insulin (Sigma-Aldrich), and MHHNB11 medium was supplemented with MEM Non-Essential Amino Acids (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... Human interferon-β recombinant protein was purchased from Millipore (St. Louis, MO), and for stimulation ...
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bioRxiv - Cancer Biology 2023Quote: ... PANC0813 medium was supplemented with 10units/mL human recombinant insulin (Sigma-Aldrich), and MHHNB11 medium was supplemented with MEM Non-Essential Amino Acids (Sigma-Aldrich) ...
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bioRxiv - Genetics 2019Quote: ... centrifuged 20 min at 20000 g and recombinant GST-tagged proteins were purified using the GST-BindTM Kit (Novagen). To synthetize the NOVA2 RNA target ...
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bioRxiv - Developmental Biology 2021Quote: ... The obtained antisera were affinity-purified with recombinant Emi2-His protein electroblotted onto a membrane (Immobilon; EMD Millipore).
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bioRxiv - Zoology 2020Quote: ... recombinant RhATG5 and RhATG5191-199Δ were affinity-purified using Ni-NTA His•Bind Resin (Merck-Millipore, Darmstadt, Germany). Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck ...
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bioRxiv - Developmental Biology 2022Quote: Lentiviral particles encoding Flag-tagged human PDHA1 or shRNAs targeting mouse Pdha1 (TRCN0000041914/TRCN0000041914 (Sigma)) were produced in 293T packaging cells by transient transfection using Jet-PEI reagent (Ozyme) ...
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bioRxiv - Microbiology 2020Quote: ... pLysS cells expressing His-topoIB and T18 tagged genome segregation proteins in different combinations using the kit Protein G immunoprecipitation from Sigma-Aldrich as described previously (Maurya et ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 ug inactive MEK1 (C-terminally His-tagged) in a total volume of 40 μL buffer (20 mM Hepes pH 8 (MERCK, Sigma Aldrich, catalogue No ...
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bioRxiv - Molecular Biology 2023Quote: ... Wells were washed thrice with PBST and monoclonal primary antibody (anti-his-tagged mouse antibody, 1:5000 diluted, Sigma-Aldrich, USA) was added and incubation was continued for 1 h at RT ...
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bioRxiv - Biochemistry 2024Quote: ... of GST and His tagged proteins were subjected to buffer exchange using Amicon Ultra 0.5 ml 10 kDa cutoff columns (Millipore Sigma, UFC501024) with five sequential rounds of concentration performed by centrifugation at 14000 g and 4 °C for approximately 10 minutes and dilution with “EB base” (5x ...
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bioRxiv - Molecular Biology 2024Quote: pDest17 plasmids containing an N-terminal 6x-His tag and Flag-tagged ARH3 wildtype or H182R were transformed into Rosetta (DE3)pLysS Competent Cells (Sigma, #70956). An initial 5 mL culture was grown overnight in Lennox LB broth supplemented with 100 μg/mL of ampicillin and 25 μg/mL of chloramphenicol at 37 °C while shaking at 200 rpm ...
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bioRxiv - Cell Biology 2021Quote: For conjugation of SNAP-tagged proteins (paxillin, kindlin) recombinant proteins to be labeled with fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼50 μM ...
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged recombinant protein was eluted by three sequential incubations of GSH-beads with 50mM reduced L-GSH (SIGMA, G4251) in 100mM Tris-HCl [pH8.0],150mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteases were obtained from the following vendors: human cathepsin L (Millipore Sigma, Athens Research and Technology ...
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bioRxiv - Biophysics 2020Quote: The recombinant human Mdm2 (1-125 aa) were cloned into pET11a vector (Novagen) and expressed in E ...
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bioRxiv - Cell Biology 2020Quote: ... 100 ng/ml cholera toxin and 10 μg/ml recombinant human insulin (Sigma). MCF10A I-PpoI cells and MCF10A AsiSI cells were previously described (Harding et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... JSH23 (Cat# 481408) and Recombinant human IGF-1 (Cat# GF138) purchased from Millipore; JQ1 (Cat# M2167 ...
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bioRxiv - Genomics 2022Quote: ... supplemented with 0.5 mg/mL recombinant human albumin (Sigma-Aldrich, St. Louis, MO), 213 μg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 10 ng/ml of human rIGF1 (recombinant Insulin growth factor-1) (Sigma, USA), 10 ng/ml amphotericin B ...
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bioRxiv - Cell Biology 2020Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1; Sigma-Aldrich), and 20 ng/mL human bFGF (Life Technologies)] for 14 to 35 days ...
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bioRxiv - Microbiology 2022Quote: ... and 200 nM of recombinant human purified E-cadherin (5085, Sigma-Aldrich, USA) for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... After 16 h 50 ng/ml of recombinant human (rh)TGFA (Sigma-Aldrich) and/or Afatinib (5 µM ...
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bioRxiv - Neuroscience 2023Quote: ... and human recombinant FGF-2 (10 ng/ml; Merck Millipore, Burlington, MA, USA) as mitogens ...
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bioRxiv - Developmental Biology 2022Quote: ... 50 μL of sample or standard (Recombinant human IFN-α, IFNA: Millipore, IF007) were added ...
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bioRxiv - Bioengineering 2023Quote: Insulin-azide and insulin-alkyne were prepared from recombinant human insulin (EMD Millipore) by selective acylation of the B29Lys residue as previously reported.33 ...
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bioRxiv - Immunology 2022Quote: ... were coated with 100 ng per well recombinant human ACE2 (hACE2) (Sigma-Aldrich) in PBS ...
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bioRxiv - Genetics 2022Quote: ... the beads were incubated in 10 μg/ml recombinant E-cadherin (human, Sigma) solution overnight at 4 °C with agitation ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1; Sigma-Aldrich), and 20 ng/mL human bFGF (Life Technologies)] for 14 to 35 days.
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bioRxiv - Biophysics 2023Quote: BCL-2 active humans (recombinant, expressed in E. coli) were purchased from Sigma. Navitoclax ...
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bioRxiv - Bioengineering 2024Quote: ... and 100 U/mL premium grade recombinant human IL-2 (Sigma catalog #I7908) with media changes every 2-3 days to maintain the cells at a concentration of 0.2 M cells/mL.
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bioRxiv - Plant Biology 2019Quote: ... The recombinant proteins with either FLAG-tag or His-tag were purified by corresponding immune affinity agarose beads (Sigma).
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bioRxiv - Microbiology 2022Quote: ... Recombinant SIRV2 gp21 and SiL_0190 with C-terminal His-tag were expressed using Escherichia coli RosettaTM (DE3) cells (Novagen). Protein expression was induced during logarithmic growth through the addition of 0.5mM isopropyl-thiogalactopyranoside (IPTG ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG-tagged mouse and human SLFN14 was detected with anti-FLAG MAb (1/500) (M2; Sigma), non-tagged human SLFN14 was detected with antibodies anti-SLFN14 PAb (Abcam ...
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bioRxiv - Cell Biology 2024Quote: ... respectively (Millipore Cat# HI-14K, HI-13K). Samples treated with 3 mM glucose KRBH were measured undiluted ...
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bioRxiv - Immunology 2023Quote: ... The pull-downed proteins were separated by SDS-PAGE on 10-to-20% gradient gel (ATTO) and the recombinant FLAG- tagged WT sCTLA-4 was detected by anti-FLAG M2 antibody (Sigma-Aldrich).
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bioRxiv - Microbiology 2021Quote: ... and 20 ng/mL of recombinant human IL-13 (Sigma-Aldrich, St. Louis, MO) to promote differentiation into M2 macrophages ...
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bioRxiv - Cell Biology 2019Quote: Morphogenesis was induced by the addition of recombinant human HGF (product H1404, Sigma-Aldrich) and conditioned media from NIH/3T3 cells (product CRL-1658 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... human recombinant MAO-A (hMAO-A) and MAO-B (hMAO-B) enzymes (Sigma-Aldrich) were diluted in 50 mM phosphate buffer (final protein amount ...
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bioRxiv - Evolutionary Biology 2020Quote: ... ephyrae were placed in ASW supplemented with 500 nM human recombinant insulin (Sigma I0908). Insulin was refreshed weekly ...
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bioRxiv - Cell Biology 2021Quote: Human recombinant TNFα and LPS (from Escherichia coli O111:B4) were purchased from Sigma. VCAM-1 (sc-13160) ...
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bioRxiv - Cell Biology 2023Quote: ... these were supplemented with 2 ng/ml recombinant human FGF basic protein (Sigma-Aldrich), 15 mM sodium bicarbonate ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1; Sigma-Aldrich, USA), and 20 ng/ mL human bFGF (Life Technologies)] with 10 μM Rho kinase inhibitor Y-27632 (Wako ...
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bioRxiv - Biochemistry 2021Quote: ... His-tagged CLIP-170 fragments and mutants were expressed in BL21 (DE3) and purified by the standard His-tagged purification protocol from Novagen (69670-5, Sigma-Aldrich) with the following modifications ...
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bioRxiv - Biochemistry 2023Quote: Thermal stability of BSA and HSA variants (His-tagged form) and of commercial wild type HSA and BSA (Sigma-Aldrich A1653 and A7638, respectively) was measured by nanoscale differential scanning fluorimetry (nanoDSF) ...
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bioRxiv - Genomics 2020Quote: ... 3.33 ml 75 mg/ml Human Recombinant Albumin (A0237, Sigma-Aldrich, St Louis, MO, USA), 2.5 mL 1 M lactate in 1 M HEPES (L(+)Lactic acid sodium (L7022 ...
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bioRxiv - Cell Biology 2019Quote: ... growth media was replaced with media containing 50ng/ml BMP-12 (recombinant human, Sigma Aldrich) and 10ng/ml IGF-1 (recombinant human ...
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bioRxiv - Neuroscience 2021Quote: ... cells were stimulated with human TNF recombinant (10 ng/ml) (Sigma-Aldrich Corporation, Missouri, EUA) for 24 hours.
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bioRxiv - Developmental Biology 2021Quote: ... 10 μg/ml BSA fraction V and 10 μg/ml human recombinant insulin (both Sigma). Fluorescence was measured by flow cytometry 24 h after the addition of RA.
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bioRxiv - Developmental Biology 2021Quote: ... 10 μg/ml BSA fraction V and 10 μg/ml human recombinant insulin (both Sigma)) ...