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Citations for Millipore Sigma :
201 - 250 of 4279 citations for Recombinant Human ABHD15 His tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 1 ug inactive MEK1 (C-terminally His-tagged) in a total volume of 40 μL buffer (20 mM Hepes pH 8 (MERCK, Sigma Aldrich, catalogue No ...
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bioRxiv - Molecular Biology 2023Quote: ... Wells were washed thrice with PBST and monoclonal primary antibody (anti-his-tagged mouse antibody, 1:5000 diluted, Sigma-Aldrich, USA) was added and incubation was continued for 1 h at RT ...
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bioRxiv - Biochemistry 2024Quote: ... of GST and His tagged proteins were subjected to buffer exchange using Amicon Ultra 0.5 ml 10 kDa cutoff columns (Millipore Sigma, UFC501024) with five sequential rounds of concentration performed by centrifugation at 14000 g and 4 °C for approximately 10 minutes and dilution with “EB base” (5x ...
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bioRxiv - Molecular Biology 2024Quote: pDest17 plasmids containing an N-terminal 6x-His tag and Flag-tagged ARH3 wildtype or H182R were transformed into Rosetta (DE3)pLysS Competent Cells (Sigma, #70956). An initial 5 mL culture was grown overnight in Lennox LB broth supplemented with 100 μg/mL of ampicillin and 25 μg/mL of chloramphenicol at 37 °C while shaking at 200 rpm ...
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bioRxiv - Cell Biology 2021Quote: For conjugation of SNAP-tagged proteins (paxillin, kindlin) recombinant proteins to be labeled with fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼50 μM ...
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged recombinant protein was eluted by three sequential incubations of GSH-beads with 50mM reduced L-GSH (SIGMA, G4251) in 100mM Tris-HCl [pH8.0],150mM NaCl ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant SIRV2 gp21 and SiL_0190 with C-terminal His-tag were expressed using Escherichia coli RosettaTM (DE3) cells (Novagen). Protein expression was induced during logarithmic growth through the addition of 0.5mM isopropyl-thiogalactopyranoside (IPTG ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteases were obtained from the following vendors: human cathepsin L (Millipore Sigma, Athens Research and Technology ...
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bioRxiv - Biophysics 2020Quote: The recombinant human Mdm2 (1-125 aa) were cloned into pET11a vector (Novagen) and expressed in E ...
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bioRxiv - Cell Biology 2020Quote: ... 100 ng/ml cholera toxin and 10 μg/ml recombinant human insulin (Sigma). MCF10A I-PpoI cells and MCF10A AsiSI cells were previously described (Harding et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... JSH23 (Cat# 481408) and Recombinant human IGF-1 (Cat# GF138) purchased from Millipore; JQ1 (Cat# M2167 ...
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bioRxiv - Genomics 2022Quote: ... supplemented with 0.5 mg/mL recombinant human albumin (Sigma-Aldrich, St. Louis, MO), 213 μg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 10 ng/ml of human rIGF1 (recombinant Insulin growth factor-1) (Sigma, USA), 10 ng/ml amphotericin B ...
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bioRxiv - Cell Biology 2020Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1; Sigma-Aldrich), and 20 ng/mL human bFGF (Life Technologies)] for 14 to 35 days ...
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bioRxiv - Neuroscience 2023Quote: ... and human recombinant FGF-2 (10 ng/ml; Merck Millipore, Burlington, MA, USA) as mitogens ...
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bioRxiv - Developmental Biology 2022Quote: ... 50 μL of sample or standard (Recombinant human IFN-α, IFNA: Millipore, IF007) were added ...
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bioRxiv - Bioengineering 2023Quote: Insulin-azide and insulin-alkyne were prepared from recombinant human insulin (EMD Millipore) by selective acylation of the B29Lys residue as previously reported.33 ...
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bioRxiv - Cell Biology 2022Quote: ... After 16 h 50 ng/ml of recombinant human (rh)TGFA (Sigma-Aldrich) and/or Afatinib (5 µM ...
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bioRxiv - Biophysics 2023Quote: BCL-2 active humans (recombinant, expressed in E. coli) were purchased from Sigma. Navitoclax ...
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bioRxiv - Microbiology 2022Quote: ... and 200 nM of recombinant human purified E-cadherin (5085, Sigma-Aldrich, USA) for 1 h at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... were coated with 100 ng per well recombinant human ACE2 (hACE2) (Sigma-Aldrich) in PBS ...
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bioRxiv - Genetics 2022Quote: ... the beads were incubated in 10 μg/ml recombinant E-cadherin (human, Sigma) solution overnight at 4 °C with agitation ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1; Sigma-Aldrich), and 20 ng/mL human bFGF (Life Technologies)] for 14 to 35 days.
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bioRxiv - Bioengineering 2024Quote: ... and 100 U/mL premium grade recombinant human IL-2 (Sigma catalog #I7908) with media changes every 2-3 days to maintain the cells at a concentration of 0.2 M cells/mL.
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bioRxiv - Cancer Biology 2024Quote: ... 20 ng/ml human recombinant epidermal growth factor (EGF, Sigma Aldrich Cat#E9644) and 20 ng/ml bFGF at 37 °C in an atmosphere containing 5% CO2 ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG-tagged mouse and human SLFN14 was detected with anti-FLAG MAb (1/500) (M2; Sigma), non-tagged human SLFN14 was detected with antibodies anti-SLFN14 PAb (Abcam ...
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bioRxiv - Immunology 2023Quote: ... The pull-downed proteins were separated by SDS-PAGE on 10-to-20% gradient gel (ATTO) and the recombinant FLAG- tagged WT sCTLA-4 was detected by anti-FLAG M2 antibody (Sigma-Aldrich).
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bioRxiv - Microbiology 2021Quote: ... and 20 ng/mL of recombinant human IL-13 (Sigma-Aldrich, St. Louis, MO) to promote differentiation into M2 macrophages ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... human recombinant MAO-A (hMAO-A) and MAO-B (hMAO-B) enzymes (Sigma-Aldrich) were diluted in 50 mM phosphate buffer (final protein amount ...
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bioRxiv - Evolutionary Biology 2020Quote: ... ephyrae were placed in ASW supplemented with 500 nM human recombinant insulin (Sigma I0908). Insulin was refreshed weekly ...
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bioRxiv - Cell Biology 2021Quote: Human recombinant TNFα and LPS (from Escherichia coli O111:B4) were purchased from Sigma. VCAM-1 (sc-13160) ...
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bioRxiv - Cell Biology 2023Quote: ... these were supplemented with 2 ng/ml recombinant human FGF basic protein (Sigma-Aldrich), 15 mM sodium bicarbonate ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1; Sigma-Aldrich, USA), and 20 ng/ mL human bFGF (Life Technologies)] with 10 μM Rho kinase inhibitor Y-27632 (Wako ...
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bioRxiv - Immunology 2024Quote: ... Cells were treated with 100 ng/ml recombinant human IL-13 (SRP3274, Sigma–Aldrich) or 50 µM acid-activated omeprazole (O104 ...
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bioRxiv - Biochemistry 2021Quote: ... His-tagged CLIP-170 fragments and mutants were expressed in BL21 (DE3) and purified by the standard His-tagged purification protocol from Novagen (69670-5, Sigma-Aldrich) with the following modifications ...
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bioRxiv - Biochemistry 2023Quote: Thermal stability of BSA and HSA variants (His-tagged form) and of commercial wild type HSA and BSA (Sigma-Aldrich A1653 and A7638, respectively) was measured by nanoscale differential scanning fluorimetry (nanoDSF) ...
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bioRxiv - Genomics 2020Quote: ... 3.33 ml 75 mg/ml Human Recombinant Albumin (A0237, Sigma-Aldrich, St Louis, MO, USA), 2.5 mL 1 M lactate in 1 M HEPES (L(+)Lactic acid sodium (L7022 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were stimulated with human TNF recombinant (10 ng/ml) (Sigma-Aldrich Corporation, Missouri, EUA) for 24 hours.
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bioRxiv - Developmental Biology 2021Quote: ... 10 μg/ml BSA fraction V and 10 μg/ml human recombinant insulin (both Sigma). Fluorescence was measured by flow cytometry 24 h after the addition of RA.
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bioRxiv - Developmental Biology 2021Quote: ... 10 μg/ml BSA fraction V and 10 μg/ml human recombinant insulin (both Sigma)) ...
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bioRxiv - Microbiology 2022Quote: ... 500ng of the spike protein was treated with 100ng recombinant human cathepsin-L (Sigma-Aldrich) in sodium acetate buffer (pH 5.5 ...
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bioRxiv - Developmental Biology 2021Quote: ... Netherlands) and 48 hours later with 7.5 IU recombinant human chorionic gonadotrophin/hCG (Sigma-Aldrich, St ...
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bioRxiv - Cancer Biology 2022Quote: ... and 25 ng/ml of recombinant human Epidermal Growth Factor (EGF) (Sigma, Sitnyakovo Blvd, Bulgaria). Cells were then filtered through a 100 μm filter to retain the non-digested fragments ...
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bioRxiv - Cancer Biology 2023Quote: ... in the presence or absence of 100 pg/ml recombinant human (rh)-IL21 (Sigma-Aldrich). The next day ...
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bioRxiv - Genomics 2023Quote: ... 3.33 ml 75 mg/ ml Human Recombinant Albumin (A0237, Sigma-Aldrich, St Louis, MO, USA), 2.5 mL 1 M lactate in 1 M HEPES (L(+)Lactic acid sodium (L7022 ...
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bioRxiv - Microbiology 2022Quote: ... and 25 µg mL−1 of recombinant human purified E-cadherin (5085, Sigma-Aldrich, USA) in PBS for 1 h at 37 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... treated twice every 12 h with human recombinant sDLL4 (1µg/ml, Cat#: SRP3026, Sigma Aldrich) for a total of 24h ...
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bioRxiv - Microbiology 2024Quote: ... one well of each cell type was treated with recombinant human IFNβ (Millipore Sigma, #IF014) diluted to 100 U/ml in appropriate media ...
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bioRxiv - Microbiology 2024Quote: Human recombinant DNase I with a specific activity of 2,500 Kunitz units/mg (Sigma-Aldrich) and RNase A (Sigma-Aldrich ...
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bioRxiv - Genetics 2024Quote: ... 3.33 ml 75 mg/ml Human Recombinant Albumin (A0237, Sigma-Aldrich, St Louis, MO, USA), 2.5 mL 1 M lactate in 1 M HEPES (L(+)Lactic acid sodium (L7022 ...