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Citations for Millipore Sigma :
201 - 250 of 3268 citations for GDF 11 BMP 11 Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... MOLM-13 and MV-4-11 cells were maintained in Roswell Park Memorial Institute 1640 media (RPMI; Sigma-Aldrich) supplemented with 2mM GlutaMAX and 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... IE were maintained in complete RPMI 1640 medium with 11 mM glucose and 0.2% sodium bicarbonate (R-0883, Sigma) supplemented with 5% human serum ...
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bioRxiv - Cell Biology 2020Quote: ... After digestion at 37 °C for 11 min and density gradient centrifugation with Histopaque-1077 (Sigma-Aldrich, St.Louis, MO, USA) for 23 min ...
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bioRxiv - Molecular Biology 2022Quote: ... genomic DNA was extracted from 5 individual embryos at mid-gastrula (st. 11) or later employing the GenElute Mammalian Genomic DNA Miniprep Kit (Sigma). The genomic region containing the CRISPR / Cas9 targeted region was PCR amplified using a nested PCR approach (Table 1 ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then fixed and rat anti-MBP (1/5) [11] and rabbit anti-NF-H (1/1000, EMD Millipore) primary antibodies were added o/n at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... Synechocystis PCC6803 (glucose tolerant, obtained from K. Hellingwerf, UvA, Amsterdam) cells were maintained at 30°C on BG-11 media (Sigma) supplemented with 10 mM TES-NaOH (pH 8.2) ...
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Cell culture dimensionality influences mesenchymal stem cell fate through cadherin-2 and cadherin-11bioRxiv - Cell Biology 2019Quote: pLKO.1 plasmids containing short hairpin RNA (shRNA) sequences targeting cadherin-11 or cadherin-2 were obtained from Sigma-Aldrich together with a scrambled negative control ...
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bioRxiv - Genomics 2019Quote: ... 1.5 g/L (days 0-5 and days 11-28) or 2.5 g/L (days 6-10) NaHCO3 (Sigma-Aldrich), and 0.25 mM (days 3-10 ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 isolate nCoV-WA1-2020 (MN985325.1)11 (Vero passage 3) was kindly provided by CDC and propagated once in Vero E6 cells in DMEM (Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: ... Samples with equal amounts of total protein were separated in 11% SDS-polyacrylamide gel before electrophoretic transfer onto Immobilon-P membranes (#IPVH00010; Millipore). Membranes were cut horizontally ...
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bioRxiv - Biophysics 2020Quote: ... 293T cells were transfected with 2.5 μg of ΔEnv IN-HIV-1 plasmid (DHIV3-GFP-D116G)11 using 10 μg of polyethylenimine (PEI, branched, MW ∼25,000, Sigma-Aldrich). After 20 h ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein (11 µg) were run on a 10% SDS-PAGE gel and transferred to a polyvinylidene difluoride membrane (Millipore) overnight ...
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bioRxiv - Neuroscience 2019Quote: ... 20 μg per lane Figure S3a) were separated in 11% SDS-polyacrylamide gel before electrophoretic transfer onto Immobilon-P membranes (#IPVH00010; Millipore). Membranes were cut horizontally ...
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bioRxiv - Synthetic Biology 2021Quote: ... The natural transformation was started by adding 3.5 µL of the precultures (OD600 ∼ 9 - 11) to 350 µL sea salt medium (28 g/L (Sigma, S9883)) with 100 µM IPTG in a 1.5 mL reaction tube ...
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bioRxiv - Microbiology 2020Quote: The crushed onion and garlic were then combined with equal volumes of wine (Pennard’s organic dry white, 11% ABV, sourced from Avalon Vineyard, Shepton Mallet) and bovine bile salts (Sigma Aldrich) made up to 89 mg·ml-1 in water and sterilised by exposing to UV radiation for 10 minutes (Carlton Germicidal Cabinet fitted with a 2537Å ...
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bioRxiv - Cell Biology 2022Quote: ... CAS#96736-11-7) was used at a final concentration of 100 μg/ml and thiabendazole (TBZ) (Sigma-Aldrich; T8904) at 5-15 μg/ml in YE media where appropriate ...
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bioRxiv - Cell Biology 2022Quote: ... The wells were coated with a 100 µL solution of 10 µg/mL porcine fibronectin (prepared from whole blood) and 11 mg/mL bovine serum albumin (BSA, endotoxin- free, fatty acid free; A8806, Sigma) dissolved in Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Developmental Biology 2020Quote: ... 1.5 g/L (days 0-5 and days 11-14) or 2.5 g/L (days 6-10) NaHCO3 (Sigma-Aldrich), and 0.25 mM ascorbic acid (days 3-10) ...
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bioRxiv - Genetics 2020Quote: ... Bead-beating was done in an Axygen 1.1 ml plate (P-DW-11-C-S) with 250 μl of acid washed 425–600 μm beads (Sigma G8722) per well ...
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bioRxiv - Immunology 2020Quote: 8 to 12-weeks old female C57BL/6j were inoculated subcutaneously with 5×105 B16-F10-OVA and conditioned 11 days later with peritoneal injection of 300 mg/kg cyclophosphamide (Sigma). On day 14 ...
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bioRxiv - Immunology 2020Quote: ... overlapping 15mer peptides offset by 11 residues were designed and synthesised (Mimotopes) and reconstituted in sterile 100% DMSO (Sigma-Aldrich) at a concentration of 100 mg/mL ...
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bioRxiv - Microbiology 2020Quote: ... pounded together in a pestle and combined with equal volumes of wine (Pennard’s organic dry white, 11% ABV, Avalon Vineyard, Shepton Mallet) and bovine bile salts (Sigma Aldrich). The bovine bile salts were made up to 89 mg/mL in water and sterilised by exposing to UV radiation for ten minutes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The resulting multilamellar liposomes were extruded 11 times through two stacked polycarbonate membranes of 0.8 μm pore diameter (Whatman-Nuclepore, Sigma-Aldrich) using a custom-built high-pressure extrusion device or a syringe extruder (Avanti Polar Lipids). ...
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bioRxiv - Cancer Biology 2022Quote: ... see Figure S1A and 22) added to the cells with 400 µg/ml of poloxamer-407 (Sigma, 9003-11-6) and incubated in a 37°C CO2 incubator for another 24 hours (Figure 1A) ...
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bioRxiv - Biochemistry 2022Quote: ... coli using the pET28a T7 expression vector and were purified and crystallized as described previously.11 KPC-2E166Q crystals were soaked with solutions of 10 mM ampicillin (Sigma), 15 mM cefalothin (Sigma) ...
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bioRxiv - Microbiology 2022Quote: ... Clarified supernatants were purified by affinity chromatography using an anti-HA affinity matrix (Cat# 11 815 016 001, Millipore Sigma) pre-equilibrated with 20 mM Tris ...
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bioRxiv - Neuroscience 2022Quote: ... The sex steroid-containing feed was prepared by mixing 5 mg dry feed with 10-100 ng of 11-KT or E2 (Sigma) using 96% ethanol as the vehicle and then evaporated at 40 °C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Viral Vector Facility, University of Zurich) using a pneumatic injector (Narishige, IM-11-2) and calibrated microcapillaries (Sigma-Aldrich, P0549). During the same surgery ...
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bioRxiv - Bioengineering 2023Quote: The decolorization of indigo carmine by hydrogels was determined by suspending an 83 mm3 subsection of a hydrogel (two interconnected hollow squares) in a 500 μL solution of BG-11 media with 0.1 mM indigo carmine (Sigma-Aldrich). Indigo carmine concentrations were determined spectrophotometrically by generating a standard curve for absorbance at 612 nm for 200 μL samples in a 96-well plate with a Tecan Infinite M200 plate reader ...
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bioRxiv - Biochemistry 2023Quote: Mouse monoclonal anti-FLAG M2 (#F3165) and anti-CD95 mAb CH-11 (#05-201) was from sigma-Aldrich (Sigma Aldrich Chimie ...
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bioRxiv - Cell Biology 2023Quote: ... The NF-κB activation inhibitor BAY 11-7085 and lipopolysaccharides (LPS) from Escherichia coli O55:B5 were from Sigma Aldrich. Recombinant human vascular endothelial growth factor-165 (VEGF ...
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bioRxiv - Microbiology 2023Quote: ... Human neuroblastoma cell line SK-N-SH (ATCC HTB-11) was cultured in Dulbecco’s Modified Eagle’s Medium with 1g/L glucose (Sigma-Aldrich, FIN) supplied with 10 % FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 and 7 post IL-12mRNA/scRNA injection (day 11, 13 and 17 post inoculation) and digested in 30% collagenase (Sigma) for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... the water filter was replaced with a filter paper with 10 μL of 1% TMT (Sigma, CAS:13623-11-50). Mice were then recorded for an additional 5 min to analyze the test trial with TMT ...
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bioRxiv - Synthetic Biology 2024Quote: ... The natural transformation was started by adding 3.5 µL of the precultures (OD600 ∼ 9 – 11) to 350 µL sea salt medium (28 g/L (Sigma, S9883)) with 100 µM IPTG and 100 ng of tDNA in a 1.5 mL reaction tube ...
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bioRxiv - Cell Biology 2024Quote: ... CAS#96736-11-7) was used at a final concentration of 100 μg/ml and thiabendazole (TBZ) (Sigma-Aldrich; T8904) at 5-15 μg/ml in YE media where appropriate ...
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bioRxiv - Cell Biology 2019Quote: ... supplemented with 2 nM BMP-2 (Sigma-Aldrich) for two weeks ...
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bioRxiv - Cancer Biology 2021Quote: ... or DMH1 (BMP receptor, Sigma #D8946, 0.5 µM) or EC330 (LIF receptor ...
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bioRxiv - Bioengineering 2024Quote: ... BMP-2 (50 ng/ml; Sigma-Aldrich, H4791) in PBS was used for 2 h at RT ...
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bioRxiv - Immunology 2020Quote: ... The bioswitch labeling procedure was performed according to the protocols described in (11, 45) with modifications using N-ethylmaleimide (NEM, Sigma Aldrich) to alkylate the reduced thiols ...
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bioRxiv - Genetics 2019Quote: ... We used the anti-HA high affinity monoclonal antibody from rat to recognize the HA-peptide (clone 3F10, ref 11 867 423 001, Sigma-Aldrich).
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bioRxiv - Neuroscience 2021Quote: ... Retinaloxime standards were prepared from 11-cis retinal using published methods(Garwin & Saari, 2000) and retinyl palmitate for use as standard was from Sigma Aldrich Inc ...
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bioRxiv - Physiology 2021Quote: ... female C57BL/6J mice (age 10-11 weeks) were given 2mg/ml IDO1 inhibitor (1-methyl-L-tryptopham, L1MT; Sigma-Aldrich) in drinking water supplemented with Splenda sweetener (2 sachets/liter ...
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bioRxiv - Bioengineering 2021Quote: ... once with a 1% SDS-TRIS pH 11 solution (5% v/v of 20% sodium dodecyl sulfate #05030, from Sigma Aldrich and 2.4 % w/v TRIS base PBP151-500 from Fisher Scientific in reverse osmosis water ...
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bioRxiv - Molecular Biology 2021Quote: Exponentially growing Jurkat Tat KB001 or mdHIV clone 11 cells (3×107 cells/IP) were fixed with 1% formaldehyde (Sigma-Aldrich) for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... 2×106 MOLM-14 or MV4-11 cells were resuspended in a medium containing 8μg/mL polybrene (Sigma-Aldrich, Cat# 107689) and 2 mL of thawed lentivirus-containing HEK 293T/17 supernatant ...
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bioRxiv - Microbiology 2021Quote: ... the anoxic treatments comprised incubations to which either 11 µM of sodium sulfide (Na2S*9H2O; Sigma-Aldrich, St. Louis, MS, USA) was added (anoxic-sulfidic ...
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bioRxiv - Bioengineering 2022Quote: ... the 11-mercaptoundecanoic acid (CAS no: 71310-21-9) and L-Tryptophan (CAS no: 73-22-3) were obtained from Sigma Aldrich. The RBD ...
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bioRxiv - Biochemistry 2019Quote: All image-based experiments were conducted on L1 animals which were collected from mixed-stage plates and isolated using a 96 well 11 µM Multiscreen Nylon Mesh filter plate (Millipore: S5EJ008M04) as described previously (Spensley et al. ...
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bioRxiv - Neuroscience 2021Quote: ... 4-6 flies were introduced one by one into 5 cm diameter circular arena with sloped edges (11°) covered with glass that was pre-coated with Sigmacote (SIGMA-ALDRICH). This arena triggers an escape reflex to the point that lifting the glass cover allows flies to immediately take-off from the open container ...