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Citations for Millipore Sigma :
201 - 250 of 10000+ citations for Dengue Virus Serotype 1 DIII envelope protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Virus amplification was performed in Sf9 TriEX cells (Novagen / Merck #71023-3) grown in TriEx media (Novagen / Merck #71022) ...
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bioRxiv - Immunology 2022Quote: ... virus particles were precipitated by adding 50% (w/v) PEG6000 (Sigma-Aldrich) in NT buffer (150 mM NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... Virus was backfilled into a pipette of mineral oil (M5904, Sigma-Aldrich). We injected 400nl virus into the identified C2 barrel column through a single injection site over 4 min at 300μm below pia ...
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bioRxiv - Immunology 2020Quote: Inactivated virus was concentrated using Amicon Ultra Centrifugal Filter units (Millipore Sigma) at 100k cutoff ...
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bioRxiv - Microbiology 2020Quote: ... Unabsorbed virus were removed and cells were overlaid with MEM (Sigma-Aldrich) containing 0.65% agarose (Nacalai tesque ...
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bioRxiv - Neuroscience 2023Quote: ... The virus-infected GL261 cells were trypsinized with 0.25% trypsin (Sigma-Aldrich) 72 hours after infection ...
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bioRxiv - Cell Biology 2023Quote: ... Virus was then filtered with a 0.45 µm PVDF filter (SLHV013SL, Millipore) and mixed with 4-fold volume of fresh media containing 12.5 µg/ml polybrene (TR-1003-G ...
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bioRxiv - Cell Biology 2023Quote: ... Virus was then filtered with a 0.45 µm PVDF filter (SLHV013SL, Millipore) and mixed with 4-fold volume of fresh media containing 12.5 µg/ml polybrene (TR-1003-G ...
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bioRxiv - Cancer Biology 2023Quote: ... An empty TRC1 lentiviral construct was used as the Control virus (Sigma).
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bioRxiv - Cancer Biology 2023Quote: ... The virus was concentrated using Amicon Ultra filters (Millipore cat. no. UFC910024) until they have about 1.5ml of concentrated virus ...
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bioRxiv - Cell Biology 2023Quote: ... where cells and virus were mixed with polybrene (Millipore, TR-1003-G) in antibiotic-free media at 2 M cells per well of a 12-well plate ...
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bioRxiv - Cell Biology 2023Quote: ... where cells and virus were mixed with polybrene (Millipore TR-1003-G) in antibiotic-free media at 2 M cells per well of a 12-well plate ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transduced with virus along with 10 μg/mL polybrene (Sigma). Following transduction ...
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bioRxiv - Genomics 2024Quote: ... virus-containing supernatant was filtered through a 0.45 μm filter (Merck Millipore), aliquoted ...
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bioRxiv - Immunology 2019Quote: ... duplicate wells of a 12-well plate were seeded with 1 ml/well of the cell suspension (1 × 106 cells/ml) and virus was added in the presence of 6 μg/ml polybrene (Sigma-Aldrich, #107689) (multiplicity of infection of 25) ...
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bioRxiv - Cell Biology 2022Quote: ... the virus-containing supernatant was filtered and used to infect WM115 cells in the presence of 1 μg/mL polybrene (Sigma-Aldrich, H9268). This was repeated with fresh viral supernatant 48 hours later ...
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bioRxiv - Pathology 2022Quote: ... and a mouse monoclonal antibody raised against Simian Virus-40 (SV-40, Macaca mulatta polyomavirus-1, Betapolyomavirus macacae) LT antigen (CalBiochem®, Clone PAb416, Sigma-Aldrich) was used as primary antibody at a 1:200 dilution ...
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bioRxiv - Microbiology 2022Quote: ... beginning with a virus stock dilution of 1:5 in complete DMEM media as mentioned above supplemented with DEAE-Dextran (Sigma Aldrich #30461) at the final concentration of 40 mg /ml ...
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bioRxiv - Systems Biology 2023Quote: ... K562 cells were transduced in 6-well plates by mixing the cells and virus with 1:500 dilution of 4 mg/mL Polybrene (Sigma H9268-5G). For spinfection ...
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bioRxiv - Cancer Biology 2021Quote: ... phosphorylated SR proteins (clone 1H4, 1:500, Millipore, #MABE50 ...
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bioRxiv - Systems Biology 2021Quote: ... YeastBusterTM Protein Extraction Reagent with 1% THP (Novagen) was added to the human cell samples and DPBS supplemented with protease inhibitor was added to the yeast samples (both as 50% of final volume) ...
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bioRxiv - Neuroscience 2021Quote: ... Synaptic vesicle protein 2A (1:500; Millipore GR09L), Syntaxin-1a (1:500 ...
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bioRxiv - Biochemistry 2019Quote: ... anti-Protein A antibodies (Sigma, P3775, 1:5000) and anti-La antibodies (a gift from C ...
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bioRxiv - Pathology 2021Quote: ... pro-surfactant protein C (Millipore AB3786, 1:500), p53 (Abcam ab131442 ...
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bioRxiv - Neuroscience 2022Quote: ... Amyloid β Protein Fragment 1-42 (A9810, Sigma) was diluted at 1 µg/mL in PBS and passively absorbed on multiwell plates (SpectraPlate-384 HB ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-Protein A antibodies (Sigma, P3775, 1:5000). IRDye secondary antibodies (800CW and 680RD ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were reduced using 1 mM DTT (Sigma) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... HEK-293T cells were cotransfected with gene-of-interest (GOI)-containing vectors with doxycycline dependent promoter and components of 2nd generation packaging vectors: pPAX2 packaging vector and pMD2G envelope vector using linear PEI 25000 (SIGMA-ALDRICH). 48 h post-transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... Retroviruses were packaged by co-transfection of Gag-Pol expressing 293 T cells with expression constructs and envelope vectors (VSV-G) using polyethylenimine (PEI; Sigma-Aldrich). Following transduction with Ccl2 or control Empty constructs ...
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bioRxiv - Cancer Biology 2022Quote: ... Retroviruses were packaged by co-transfection of Gag-Pol expressing 293 T cells with expression constructs and envelope vectors (VSV-G) using polyethylenimine (PEI; Sigma-Aldrich). Following transduction with shRNA retroviral constructs ...
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bioRxiv - Biochemistry 2019Quote: ... 50 ng of envelope plasmid (pCMV-VSV-G) and 500 ng of pLKO vector containing the hairpin sequence (Sigma; Table S2) using TransIT-LT1 (Mirus Bio ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were mixed with 200uL of pre-washed slurry of a 1:1 mix of EZview Red Protein G and EZview Red Protein A bead affinity gel slurry (Sigma-Aldrich) and rotated at 4C for 1hr ...
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bioRxiv - Cell Biology 2023Quote: ... protein inhibitor cocktail (1:100) and Benzonase nuclease (Novagen, 70746, 1:100) in PBS ...
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bioRxiv - Genomics 2023Quote: The lentiviral titer was determined by transducing 400,000 cells with increasing volumes (0, 1, 2, 5, 10, 20, and 40 μl) of concentrated virus and polybrene (6 μg/ml; Millipore, TR-1003-G). 48 hours after the transduction ...
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bioRxiv - Microbiology 2024Quote: ... ½-log serially diluted influenza viruses in virus growth medium plus trypsin (DMEM, 0.1% BSA, 1 µg/mL TPCK-treated trypsin (Sigma, St. Louis, MO, USA)) were added to confluent monolayers of MDCK-SIAT1 cells (37 ...
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Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: ... Virus was then concentrated using Amicon Ultra-4 centrifugal filter units (Merck-Millipore) and the solution was filtered with a 0.2 µm Acrodisc syringe filter (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2019Quote: ... Virus was thawed at 37°C and 5μl 10mg/ml polybrene (Sigma H9268) was added to the virus ...
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bioRxiv - Biochemistry 2021Quote: ... Cholesteryl hemisuccinate (CHS) and tobacco etch virus (TEV) protease were from Sigma-Aldrich.
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bioRxiv - Neuroscience 2021Quote: ... Virus was then concentrated and purified via Iodixanol gradients (“Optiprep” Sigma D1556-250mL). Centrifuge the gradients for 90 min at 46,500 rpm at 16 °C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... virus containing supernatant was harvested and filtered (0.45 μm, Millex-HV Filter; Millipore). Subsequently ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were infected with virus in the presence of 8μg/ml polybrene (Sigma).
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bioRxiv - Cell Biology 2022Quote: ... Live filtered virus was used to transduce cells with polybrene (10μg/ml, Sigma).
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bioRxiv - Cell Biology 2020Quote: ... the virus was concentrated using Amicon ultra-15 centrifugal filter units (Millipore Sigma) by centrifuging at 3000 rpm and stored at -80°C until use ...
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bioRxiv - Immunology 2019Quote: ... Virus-containing supernatants were filtered through a 0.45-μm-pore-size filter (Millipore) and supplemented with polybrene (8 μg/ ml ...
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bioRxiv - Cell Biology 2019Quote: ... Virus was resuspended by fresh MEFs medium with 8 μg/ml polybrene (Sigma).
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bioRxiv - Microbiology 2020Quote: ... 100 μl of virus preparations were treated with 25 units of Benzonase (Sigma) for 30 min before RNA isolation.
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bioRxiv - Cancer Biology 2020Quote: ... the virus was harvested from the medium filtered by 0.45 mm Steriflipfilter (Millipore). Virus infection was carried out by incubating cells with cell medium containing indicated virus and 5 μg/ml polybrene (Sigma ...
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bioRxiv - Microbiology 2020Quote: Cells or virus were lysed with RIPA buffer and protease inhibitor cocktail (Sigma). Protein loaded from viral lysates were normalized to p24 antigen content ...
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bioRxiv - Immunology 2021Quote: ... Virus and cell pellets were solubilized in lysis buffer [10mM iodoacetamide (Sigma-Aldrich), CompleteTM protease inhibitor tablets (Roche) ...
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bioRxiv - Immunology 2021Quote: ... The virus-plasma-cell mixtures were plated in white polystyrene plates (Millipore-Sigma) and cultured at 37°C 5% CO2 ...