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Citations for Millipore Sigma :
201 - 250 of 10000+ citations for 7 Chloro 2 hydrazino 5 phenyl 3H 1 4 benzodiazepine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... and then incubated with nitro blue tetrazolium chloride/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) substrates (Sigma-Aldrich) in NTM (100mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... and the blotting signals were chemically visualized with either the nitro-blue tetrazolium/5-bromo-4-chloro-3′-indolyphosphate (NBT/BCIP) chromogenic assay (Sigma) or infrared scanner ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: ... coli DH5α cells were transformed with the resulting pho-lac fusion plasmids and streaked on dual indicator plates containing LB agar with 5-bromo-4-chloro-3-indolyl phosphate disodium salt (X-Phos) (Sigma) at a final concentration of 80μg/ml and 6-chloro-3-indolyl-β-D-galactoside (Red-Gal ...
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bioRxiv - Microbiology 2021Quote: ... Immunoreactive bands were detected by the addition of BCIP (5-bromo-4-chloro-3-indolylphosphate)-nitroblue tetrazolium solution (Sigma-Aldrich). The reaction was stopped after 2 min by washing the blots with large volumes of deionized water.
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bioRxiv - Bioengineering 2021Quote: ... slides were either treated with SIGMA FAST™ BCIP/NBT (5-Bromo-4-chloro-3-indolulphosphate/Nitro blue tetrazolium, pH 9.5, Sigma) and counterstained with nuclear fast red (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... Color was developed with freshly prepared 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium (BCIP/NBT; Sigma-Aldrich) and the reaction was stopped by addition of 2 mM ethylenediaminetetraacetic acid (EDTA).
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bioRxiv - Microbiology 2023Quote: ... the blots were washed again using TBST and developed using AP-reactive Nitrobluetetrazolium (NBT) 5-bromo-4-chloro-3-indolylphosphate (BCIP) tablets (Sigma). A final image of the blots was taken on a Bio-Rad ChemiDoc gel imaging system using colorimetric detection.
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bioRxiv - Developmental Biology 2024Quote: ... the samples were incubated in NBT (nitro-tetrazolium blue)-BCIP (5 bromo-4-chloro-3’-indolyphosphate p-toluidine) (Sigma-Aldrich), 0.033% in APTMg in dark condition ...
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bioRxiv - Immunology 2021Quote: ... the γ-secretase inhibitor (2S)-N-[(3,5-Difluorophenyl)acetyl]-L-alanyl-2-phenyl]glycine 1,1-dimethylethyl ester (DAPT; R&D) or dimethyl sulfoxide (DMSO; Sigma) was added to media at the concentrations indicated.
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bioRxiv - Cancer Biology 2024Quote: ... Embryos were transferred to E3 solution containing 5 µg/ml proteases and 0.2 mM1-phenyl-2-thio-urea (Sigma) 24 h post-fertilization to dechorionate the fish embryos and prevent pigmentation ...
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bioRxiv - Neuroscience 2022Quote: ... Mice were sacrificed under anesthesia with 2-bromo-2-chloro-1,1,1-trifluoro-ethane (Sigma) by cervical dislocation ...
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bioRxiv - Developmental Biology 2022Quote: ... 4% 7-DHC (Sigma)) and 50-200 μL fresh baker’s yeast as food source and egg laying substrate on top ...
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bioRxiv - Biochemistry 2019Quote: 2 µl of 1 mM 2’,7’ Dichlorofluorescein diacetate (Sigma-Aldrich) dissolved in DMSO was added to 198 µl of cell culture in a black f-bottom 96 well plate (Greiner bio-one ...
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bioRxiv - Neuroscience 2020Quote: ... The signal was determined by nitroblue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (toluidine salt) (BCIP) (Sigma, 72091).Semithin sections for toluidine blue myelin staining and ultrathin section for TEM imaging were prepared according to our previous protocols (Zhang et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... at 60°C and developed using nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) (Sigma Aldrich) for 60 minutes ...
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bioRxiv - Microbiology 2020Quote: ... a 1:10,000 anti-rabbit dilution of IgG alkaline phosphatase was used before detection with NBP-BCIP (nitroblue tetrazolium chloride–5-bromo-4-chloro-3′-indolylphosphate; Sigma-Aldrich) as the substrate.
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bioRxiv - Plant Biology 2021Quote: ... immunodetection of the DIG-labelled probes was carried out by using an anti-DIG antibody coupled with alkaline phosphatase and a ready-to-use 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro-blue tetrazolium (NBT) solution (Sigma-Aldrich) as the chromogenic substrate for the enzyme ...
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bioRxiv - Developmental Biology 2020Quote: ... The staining reactions lasted between 2h and 8h at room temperature using nitro-blue tetrazolium (NBT) and 5-bromo-4-chloro-3′-indolyphosphate (BCIP) staining reactions (Sigma Aldrich). Embryos were then rinsed in PBS-T (0.05% Tween-20) ...
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bioRxiv - Plant Biology 2020Quote: ... and placed in Petri dishes filled with 0.5% agarose containing 35 mM β-mercaptoethanol (v/v) and 1.5 mg/mL of 5-bromo-4-chloro-3-indolyl-β-D-galactoside (Sigma). For X-gal staining ...
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bioRxiv - Microbiology 2022Quote: ... Detection was performed by colorimetry using nitro blue tetrazolium chloride / 5-bromo-4-chloro-3-indolyl-phosphate (NBT-BCIP, Sigma-Aldrich) as a substrate.
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bioRxiv - Molecular Biology 2023Quote: ... the cells were washed four to five times with warm media (DME-HG for MEFs and DME-HG/F12 for MCF10A) and pulse-labeled with 100 µM 5- chloro-2’-deoxyuridine (CldU; Sigma) for 20 min at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos derived from rpe65a:nfsB-eGFP crosses were treated with 1-phenyl-2-thiourea (PTU; Sigma-Aldrich) from 6hpf to 5dpf to inhibit pigmentation ...
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bioRxiv - Developmental Biology 2019Quote: ... medium was supplemented with 8 mM PTU (1-phenyl-2-thiourea; Sigma-Aldrich, St. Louis, MO) to avoid formation of melanin pigments ...
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bioRxiv - Developmental Biology 2021Quote: ... which was renewed daily and supplemented with 0.2 mM 1-phenyl-2-thiourea (PTU) (Sigma-Aldrich) from 8±1 hpf onwards to prevent pigmentation ...
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bioRxiv - Developmental Biology 2022Quote: ... 2011) and rpe65a:nfsB-eGFP outcrosses were treated with 0.003% 1-phenyl 2-thiourea (PTU; Sigma-Aldrich) from 6hpf to 5dpf to prevent pigmentation ...
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bioRxiv - Microbiology 2022Quote: ... embryos were maintained in 0.5X E2 medium (80) with 0.003% PTU (1-phenyl-2-thiourea, Sigma).
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated with 10μM 1,2-dioleoyl-sn-glycero-3-phospho-L-serine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (18:1 NBD-PS) (1:300, Sigma-Aldrich) for 15 minutes at 25°C ...
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bioRxiv - Neuroscience 2020Quote: ... were performed in DJ-1-deficient cells with MTT (3-(4, 5-dimethylthiazol-2-yl)−2–5-diphenyltetrazolium bromide) (Sigma-Aldrich) as described in [24] ...
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bioRxiv - Genomics 2020Quote: ... 4-(1,1,3,3-tetramethylbutyl)phenyl-polyethylene glycol (Triton X-100, Sigma-Aldrich, X100), Bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2020Quote: ... 5 μM [3H]-amino acid (L-Phe) and 1 μM tRNAPhe from brewer’s yeast (Sigma, US). In each experiment ...
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bioRxiv - Developmental Biology 2023Quote: Embryos used later than 34 hours post-fertilization were kept transparent by soaking them in embryo medium (Danieau’s Solution) with 1% 1-phenyl-2-thiourea (PTU) (Sigma) to inhibit pigment formation (M. ...
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bioRxiv - Microbiology 2021Quote: ... Immediately prior to plating on agar plates containing 20 mM 4-chloro-phenylalanine (4-CP; Sigma-Aldrich), the cells were washed twice with phosphate buffered saline (PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... The bound probe was visualized using 5-bromo-4-chloro-3 indoyl phosphate (NBT/BCIP; Sigma-Aldrich Corp., St. Louis, MO, USA). Bright field images were captured on the A1+ Nikon confocal microscope fitted with a DS-Ri1-U3 color camera at 20x magnification and 4076×3116 pixel resolution ...
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bioRxiv - Plant Biology 2020Quote: ... Detection was performed by adding 200 μl of western blue substrate (SIGMAFAST™ BCIP®/NBT = 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium, Sigma) followed by incubation at room temperature in a dark and moist environment until the reagent’s color became visible ...
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bioRxiv - Neuroscience 2023Quote: ... Fixed cells were incubated with staining solution (1mg/mL of X-Gal,5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside; Sigma Aldrich #11680293001)(Lorenzo ...
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bioRxiv - Bioengineering 2023Quote: Nuclear DNA integrity and gross cell morphology in dPPM was assessed with 4’,6’- diamidino - 2 - phenyl indole (DAPI, Sigma-Aldrich) staining and fluorescence microscopy (Nikon Eclipse Ti ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were seeded on a 18×24 mm coverslip and the next day they were pulse-labeled with CldU (5-Chloro-2’-deoxyuridine, Millipore Sigma) and IdU (5-Iodo-2’-deoxyuridine ...
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bioRxiv - Cancer Biology 2023Quote: CRISPR-Cas13d or Tet-On overexpressing cells were sequentially pulse-labeled with 50 µM 5-chloro-2′-deoxyuridine (CldU, Sigma-Aldrich) and 250 µM 5-iodo-2′-deoxyuridine (IdU ...
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bioRxiv - Neuroscience 2022Quote: ... a selective antagonist of the benzodiazepine-site of the GABAAR was purchased from Sigma-Aldrich and dissolved in sterile HEPES buffer supplemented with KCl (2.5 mmol/L ...
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bioRxiv - Immunology 2023Quote: ... for 1 hour and then alkaline phosphatase substrate solution containing 4-nitro-phenyl phosphate (N2765-50TAB, Sigma-Aldrich) for approximately 30min ...
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bioRxiv - Developmental Biology 2021Quote: In situ hybridizations (ISH) were performed on larvae treated with 1-phenyl-2-thiourea (PTU, Sigma-Aldrich) and fixed in freshly made 4% paraformaldehyde (PFA ...
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bioRxiv - Cell Biology 2019Quote: ... From 8 hpf the medium was supplemented with 0.2 mM 1-phenyl-2-thiourea (PTU) (Sigma-Aldrich) to prevent pigmentation ...
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bioRxiv - Neuroscience 2021Quote: For whole-mount ISH embryos were treated with E3 containing 0.2mMPTU (1-phenyl-2 thiourea; Sigma-Aldrich) to avoid pigmentation ...
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bioRxiv - Developmental Biology 2022Quote: ... or 32°C in E3 medium supplemented with 0.2 mM 1-phenyl-2-thiourea (PTU, Sigma-Aldrich) from 8 hours post fertilization (hpf ...
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bioRxiv - Developmental Biology 2023Quote: ... the pigmentation of embryos was suppressed by the addition of 1-phenyl-2-thiourea (PTU) (Sigma-Aldrich) in E3 media ...
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bioRxiv - Developmental Biology 2023Quote: Zebrafish embryos were raised in 0.003% 1-phenyl-2-thiourea (PTU, phenylthiocarbamide, or n-phenylthiourea, Sigma P7629) from gastrulation stage to prevent pigmentation ...
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bioRxiv - Developmental Biology 2024Quote: ... incubated at 28.5 °C and treated at 24 hpf with 1-phenyl-2-thiourea (PTU) (Sigma Aldrich) to inhibit pigmentation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Bixafen, (N-(3’,4’-dichloro-5-fluorobiphenyl-2-yl)-3-(difluoromethyl)-1-methylpyrazole-4-carboxamide) (Bixafen, (Sigma-Aldrich, St ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 3’-phosphoadenosine-5’-phosphosulfate (PAPS), and [1,2,6,7-3H(N)]dehydroepiandrosterone ([3H]DHEA, 60 Ci/ mmol) were purchased from Sigma-Aldrich (St. Louis,MO). SDS–polyacrylamide gel electrophoresis reagents were obtained from Bio-Rad (Hercules ...
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bioRxiv - Neuroscience 2020Quote: ... were performed with MTT (3-(4, 5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide) (Sigma-Aldrich) as described in Sanz et al ...