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Citations for Millipore Sigma :
2401 - 2450 of 7743 citations for Recombinant Salmon il21 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Proteins were concentrated with 30-kDa-cutoff Amicon centrifuge filters (Millipore), flash-frozen in liquid nitrogen and stored at -80°C ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... Protein extraction buffers contained 50mM HEPES (Sigma Aldrich, H0887-100 ml) and 150 mM NaCl ...
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bioRxiv - Physiology 2022Quote: ... Protein bands were visualized by chemiluminescent HRP substrate (WBKLS0500, EMD Millipore). Blots were developed with autoradiography films (HyBlot CL ...
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bioRxiv - Plant Biology 2022Quote: ... The proteins were then transferred to PVDF membranes (Immobilon-P; Millipore) and subsequently blocked with 5 % nonfat dry milk for 2 hours ...
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Construction of a constitutively active type III secretion system for heterologous protein secretionbioRxiv - Synthetic Biology 2022Quote: ... The proteins were transferred to a polyvinylidene fluoride membrane (PVDF, Millipore) using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... Cell pellets were then resuspended in RIPA protein extraction buffer (Sigma) supplemented with protease inhibitor (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... and protein concentration was measured with BCA assay (Sigma 71285-M). Then ...
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bioRxiv - Molecular Biology 2022Quote: ... and proteins were precipitated by the addition of trichloroacetic acid (TCA_SIGMA) to a final concentration of 18% ...
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bioRxiv - Immunology 2022Quote: ... or 100 µg protein + 10 µg/mL MG132 (Sigma catalog # 474787). Antigen pulses with and without inhibitors were performed for 6 hr at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... the proteins were transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, USA) that were subjected to blocking by 5% skimmed milk for 1 h at room temperature ...
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bioRxiv - Plant Biology 2022Quote: Heterologous protein expression was achieved using pET28b(+) (EMD Millipore, Burlington, MA), in which open reading frames for the enzymes were cloned into using doubly digested vectors of either BamHI/XhoI (ASAT4H) ...
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bioRxiv - Biophysics 2022Quote: ... and mouse monoclonal primary antibody against myelin basic protein antibody (Millipore) at 1:200 dilution ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells were treated with the protein synthesis inhibitor cycloheximide (Sigma) for the indicated durations before harvesting and analysis by immunoblotting.
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bioRxiv - Cell Biology 2022Quote: ... 0.5% BSA) and incubated with protein G beads (Sigma, 16-662) and antibody overnight at 4 °C with rotation ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred from gels onto PVDF membrane (EMD Millipore, IPFL00010) using the Genscript eBlot L1 wet transfer system (cat# L00686) ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were then transferred to polyvinyledene difluoride (PVDF) membranes (Millipore IPVH00010) in Phos-tag transfer buffer at 20V for 20 h at 4°C ...
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bioRxiv - Immunology 2021Quote: ... The proteins were then transferred onto polyvinylidene difluoride membranes (EMD Millipore). The membrane was blocked with PBS-0.05% Tween 20 (PBS-Tween ...
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bioRxiv - Immunology 2020Quote: ... Proteins were detected by using enhanced chemiluminescence (ECL) systems from Millipore or GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were visualized after transfer to Immobilon-FL PVDF membrane (Millipore). Membranes were blocked with Odyssey blocking buffer (LI-COR Biosciences ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The proteins were then alkylated with 50 mM iodoacetamide (Sigma Aldrich) for 30 min in the dark ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... Total protein was detected with Ponceau-S solution (Sigma-Aldrich, #P3504) or with stain-free technology (Bio-Rad) ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were precleared with protein G Affinity Gel (Sigma-Aldrich #E3403) for 1 hr on rotator at 4°C ...
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bioRxiv - Neuroscience 2021Quote: Protein was extracted from brain homogenates in RIPA buffer (Sigma, UK) containing cOmplete™ Mini Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein synthesis was measured using a DHFR assay kit (Sigma-Aldrich), which measures the oxidation of NADPH (60 mM ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins digest were carried out with 0.75 µg of trypsin (SIGMA) for 16 h at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were then transferred onto a polyvinylidene fluoride (PVDF) membrane (Millipore). After blocking with 5% milk solution ...
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bioRxiv - Microbiology 2021Quote: ... The transferred proteins were stained with Ponceau S solution (Sigma-Aldrich). The nitrocellulose membrane was soaked (blocked ...
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bioRxiv - Biophysics 2020Quote: ... Protein fractions were pooled and concentrated (10.000 MWCO Amicon; Merck-Millipore) up to 5 mL and analyzed on a HiLoad 26/60 Superdex 200 (GE ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred to polyvinylidinefluoride membranes (Millipore Corporation, Billerica, MA, USA), subsequently blocked 5% dry milk in TBST (Tris Buffered Saline with 0.1% Tween 20 ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by addition of Protein-A agarose beads (Sigma-Aldrich #1719408001) for 1 h at 4°C ...
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bioRxiv - Immunology 2020Quote: Total proteins were extracted by RIPA Buffer (R0278, Sigma-Aldrich, USA) containing protease inhibitors (HY-K0010 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by 8% resolving gel containing trichloroethanol (TCE, Sigma) to visualize proteins (Ladner et al. ...
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bioRxiv - Microbiology 2020Quote: ... The protein bands were visualized with a chemiluminescent detection reagent (Millipore).
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bioRxiv - Neuroscience 2021Quote: ... polyclonal goat anti-ribosomal protein L8 (SAB2500882, Sigma-Aldrich; 1:500), monoclonal mouse anti-β-Actin (GTX26276 ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein bands were detected using enhanced chemiluminescence (Luminata Crescendo, Merck Millipore). Membranes were re-probed with β-Actin antibody to ensure equal loading.
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bioRxiv - Physiology 2020Quote: ... Proteins were transferred from the gel onto a PVDF membrane (Millipore) at 30 V for 17 hours at 4°C.
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bioRxiv - Physiology 2021Quote: ... After probing for total protein transferred using Ponceau S (Sigma-Aldrich), membranes were blocked overnight in Odyssey Blocking Buffer (LICOR ...
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bioRxiv - Immunology 2020Quote: ... Digested glycans were cleaned using a PVDF protein-binding membrane (Millipore) and analyzed as described above.
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bioRxiv - Immunology 2020Quote: ... In the experiments using the protein translation inhibitor cycloheximide (Sigma Aldrich), CH12-F3 cells were exposed to low oxygen conditions (1% O2 ...
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bioRxiv - Biochemistry 2021Quote: ... The resolved proteins were transferred to nitrocellulose membranes (0.22 μm, Sigma) and probed with a mouse anti-Strep II monoclonal antibody (1/10,000 ...
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bioRxiv - Biochemistry 2021Quote: ... Protein samples were subsequently concentrated using Amicon 3-kDa concentrators (Millipore). The purity of each protein was detected by SDS-PAGE and western blotting ...
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bioRxiv - Systems Biology 2020Quote: ... Co-immunoprecipitated proteins were detected using anti-Flag antibody (M2 – Sigma), eGFP fusion protein levels monitored using anti-GFP antibody (ab290 – abcam ...
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bioRxiv - Neuroscience 2022Quote: ... The proteins were transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, IPVH00010). Then the membranes were blocked with 5% non-fat milk for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... proteins were purified from the infected cells on M2 agarose (Sigma) as previously described (Kim and Roeder ...
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bioRxiv - Cell Biology 2022Quote: ... bound to protein A agarose beads (11719408001, Millipore Sigma, Darmstadt, Germany), as previously done (Carbonneau et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... polyclonal rabbit anti-pro surfactant protein C (1:4000, Millipore, #AB3786), mouse monoclonal acetylated tubulin (Sigma ...
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bioRxiv - Bioengineering 2022Quote: Protein concentration was determined with use of the Bradford assay (Sigma) according to manufacturer’s manual if not explicitly mentioned otherwise ...
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bioRxiv - Bioengineering 2022Quote: ... Protein concentration was determined by the Bradford assay (Sigma-Aldrich, Europe) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: ... purified proteins were concentrated using a 10,000 MWCO spin concentrator (Millipore), their concentrations were determined based on their absorption at 280 nm ...
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bioRxiv - Biochemistry 2020Quote: ... The protein containing fractions were concentrated in an Amicon filter (Millipore) with cut off of 10 kDa (GrpE ...