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Citations for Millipore Sigma :
2401 - 2450 of 4079 citations for Recombinant Human VWC2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... with 1% human platelet poor-derived serum (HS; Sigma-Aldrich, P2918), 20 ng/mL basic FGF (bFGF ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... human specific N terminal anti-collagen VII LH7.2 (1:250 Millipore), The fluorescence images were taken using the TCS SP5 confocal laser scanning microscope (Leica) ...
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bioRxiv - Genetics 2022Quote: ... containing to a final concentration human serum 5% (Sigma H4522-100mL), heparin 2IU/ml (Sigma H3149-25KU) ...
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bioRxiv - Immunology 2023Quote: ... The human lymphoblastoid line BOLETH was obtained from Sigma (#88052031-1VL). B-cell lines were cultured in GlutaMAXTM supplemented RPMI 1640 medium further modified with 10% FBS ...
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bioRxiv - Bioengineering 2023Quote: ... stained using mouse anti-human fibronectin monoclonal antibody (Sigma-Aldrich, US) and detected with goat anti-mouse Alexa Fluor 488 secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and fibronectin from human plasma (Sigma-Aldrich F1056; 1 μg/ml). For some experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... and human samples were cryopreserved in 10% DMSO (Sigma-Aldrich #F4135) in FBS in liquid nitrogen at -196°C until use ...
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bioRxiv - Cancer Biology 2023Quote: ... PC3 human cell line was obtained from Sigma Aldrich (90112714-1VL) and cultured in DMEM medium with 10 % FBS.
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bioRxiv - Cancer Biology 2023Quote: PNT2 human cell line was purchased from Sigma Aldrich (95012613-1VL) and cultured in RPMI 1640 containing 2 mM Glutamine and 10 % Foetal Bovine Serum (FBS) ...
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bioRxiv - Cell Biology 2023Quote: ... containing 1% Human Platelet Poor Plasma-derived Serum (SH, Sigma, P2918), 20 ng/mL FGF2 and 10 uM All-trans Retinoic Acid (RA ...
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bioRxiv - Systems Biology 2023Quote: Human insulin (10 mg/ml) was from Sigma (#91077C, lot: 21M018) and stored at 4°C.
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bioRxiv - Biochemistry 2023Quote: ... USA) Albumin from human serum (HSA) was purchased from Sigma-Aldrich Canada ...
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bioRxiv - Neuroscience 2023Quote: ... purified calpain-1 from human or porcine erythrocytes (8 µg, Millipore) or human or rat recombinant calpain-2 produced by bacterial expression as described by (Elce et al. ...
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bioRxiv - Bioengineering 2024Quote: ... anti-human nuclei (HuNu; 1:100; MAB4383, Merck Millipore, MA, USA), anti-Ku80 (1:100 ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 10% (v/v) human AB-serum (Sigma Aldrich,, Germany) seeded into well culture plates and stimulated with antigens for 18 h in presence of 1 mg/ml of anti-CD40 blocking antibody (both Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... and mouse anti-human IgG4 biotin (Sigma-Aldrich, St Louis, MO), respectively ...
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bioRxiv - Cell Biology 2023Quote: ... 1% PS) with freshly added 8 ng/mL human bFGF (Sigma). The medium was changed daily ...
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bioRxiv - Cell Biology 2023Quote: ... human plasma fibronectin (1mg/mL overnight at 37◦C; FC010, Millipore) or adhesion protein ...
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bioRxiv - Immunology 2024Quote: ... Anti-Human IgG-Peroxidase antibody (Fab specific) produced in goat (Sigma) diluted 1:45,000 in dilution buffer for RBD and NTD plates ...
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bioRxiv - Cell Biology 2024Quote: ... Standard curves were prepared using purified human VLDL (Millipore Sigma, LP1) diluted in same cell culture media and were used for determining the concentration of ApoB in the samples.
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bioRxiv - Cancer Biology 2024Quote: ... The human immortalized HSC line LX2 was purchased from Millipore Sigma (Cat ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 ng/mL human epidermal growth factor (hEGF, Sigma Aldrich). Medium of organoids was refreshed every 2 days ...
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bioRxiv - Genetics 2024Quote: ... CpGenome™ Human Methylated & Non-Methylated DNA standard set (Sigma#S8001M) was used to prepare a set of methylation standards (0% ...
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bioRxiv - Immunology 2024Quote: Human serum IgG (hIgG) and IgA (hIgA) were purchased from Sigma Chemical Co ...
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bioRxiv - Cancer Biology 2024Quote: ... Peroxidase-conjugated anti-human IgG (Sigma-Aldrich, St. Louis, Missouri, US) was added (1 hour ...
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bioRxiv - Cell Biology 2024Quote: HEK293 (Human Embryonic Kidney, ECACC 96121229 from Sigma-Aldrich Chemie GmbH) were cultured in DMEM (Dulbecco’s modified Eagle’s medium PAN biotech ...
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Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviaebioRxiv - Microbiology 2019Quote: ... Recombinant rMSPDHA and rMSPDHB were then expressed through 1.0 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) induction and purified using Ni-NTA His-Bind® Resin Kit (Novagen, San Diego, USA). The purified protein products were quantified by using BCA Protein Assay Kit (Thermo Scientific-Pierce ...
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bioRxiv - Plant Biology 2019Quote: ... and axr3-3 were expressed as 6X His-tag (N-terminal) fusion proteins in Escherichia coli strain Rosetta™ DE3 competent cells (Supplementary Table 4; Novagen, product code: 70954). These proteins were expressed in minimal media with 13C D-glucose and 15N ammonium chloride ...
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bioRxiv - Molecular Biology 2019Quote: ... and zinc binding proteins were eluted according to the supplied protocol using HIS-Select wash and elution buffers purchased from Sigma-Aldrich (Saint Louis, MO).
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bioRxiv - Genomics 2022Quote: ... or T-175 flasks (Hi-C, CUT&RUN) at a density of 1 × 105 cells/mL and treated with 25 nM PMA (Sigma-Aldrich, cat # P1585-1MG). After 24h ...
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bioRxiv - Cancer Biology 2021Quote: ... Kinase assays were performed as described in (20) using 200ng of purified EPHA2 recombinant protein (OriGen Technologies) or indicated concentration of purified Enolase (Sigma Aldrich), in the absence or presence of about 50ng of purified Src-GFP (or Src3A-GFP as indicated ...
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bioRxiv - Evolutionary Biology 2022Quote: ... blocked in 10% NGS in PBST at 4°C for 3 hr and immunohistochemistry steps were carried out by incubating the samples with primary anti-pH3 antibody (diluted 1:300 in blocking buffer, REF 05-817R, clone 63-1C-8, recombinant rabbit monoclonal antibody, Millipore, USA) for overnight at 4°C as previously described [37–39] ...
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bioRxiv - Cell Biology 2021Quote: ... Base media for the culture of mESCs were supplemented with 1000 units/mL ESGRO Recombinant mouse LIF protein (EMD Millipore ESG1107), and 2i small molecule inhibitors ...
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bioRxiv - Biochemistry 2021Quote: ... The membrane was cut in two (one half for the commercial and one half for the recombinant anti-FLAG™-M2) and each half was probed with either commercial (Sigma) or recombinant anti-FLAG™-M2 at 1 µg/mL in PBS for 45 minutes ...
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bioRxiv - Microbiology 2021Quote: ... both Bactrian camel and llama AEC cultures were treated with recombinant universal type I and III IFNs (100 IU/ml; Sigma Aldrich) or recombinant type III IFN (100 ng/ml ...
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bioRxiv - Microbiology 2020Quote: ... strain BL21(DE3) was used for the production of recombinant proteins expressed from the pET series of plasmids (Novagen Sigma-Aldrich). All L ...
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bioRxiv - Microbiology 2020Quote: ... strain BL21(DE3) was used for the production of recombinant proteins expressed from the pET series of plasmids (Novagen Sigma-Aldrich). All L ...
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bioRxiv - Microbiology 2020Quote: ... mouse polyclonal antibody was produced immunizing a mouse with 50 mg of purified recombinant MtEPSPS containing Freund’s incomplete adjuvant (Sigma-Aldrich, USA) (total volume of 100 µL ...
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bioRxiv - Developmental Biology 2022Quote: ... The dish was then incubated with 40 µg/mL mouse E-cadherin recombinant protein (8875-EC-050, Bio-Techne) or 40 µg/mL fibronectin (F1141, Sigma-Aldrich) at 4 □ overnight ...
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bioRxiv - Cell Biology 2022Quote: ... were used as a substrate for phospho-PKCα in a 25 µl in vitro kinase reaction using 100 ng of active recombinant PKCα enzyme (Millipore-Sigma, MA), 5 µl of a lipid activator (Millipore-Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing the respective recombinant proteins were pooled and concentrated using Amicon® Ultra-15 spin concentrators with 10 kDa cut-off (Merck Millipore). Concentrated protein pools were run on a Superdex 200 Increase 10/300 GL ...
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bioRxiv - Immunology 2020Quote: ... extracellular Cn were removed by washing 2X with PBS and the macrophages were cultured for a further 24 h in fresh growth media containing either IFNγ to maintain the M1 polarization state or 100 ng/mL recombinant interleukin-4 (IL-4; Sigma-Aldrich, St ...
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bioRxiv - Neuroscience 2021Quote: ... The recombinant LymCBP HAT domain (200 ng) were incubated with the histone H1 purified from calf thymus (Sigma-Aldrich, 1 μg) and 7.4 kBq of [14C]-acetyl-CoA (Moravek ...
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bioRxiv - Microbiology 2021Quote: ... Sublines expressing a doxycycline-inducible BZLF1 transactivator were produced by culturing 106 LCL cells with the recombinant lentivirus in presence of 8 μg/ml polybrene (TR-1003-G, Sigma-Aldrich) for 24 hours followed by replacement of the infection medium with fresh complete medium ...
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bioRxiv - Neuroscience 2020Quote: ... EAE in bone marrow transplanted male animals was induced in anesthetized 8-10 week-old animals after irradiation by subcutaneous injection at the tail base of 10μg of recombinant rat MOG together with incomplete Freud’s adjuvant 1:1 (Sigma, St Louis, MO). EAE was induced in 10-12weeks old female animals using the same protocol using 10μg MOG ...
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bioRxiv - Biochemistry 2019Quote: ... recombinant mTLR9-ECD was titrated into a solution of oligonucleotide labeled at the 5’ end with Alexa 488 Fluor (Sigma-Aldrich). The oligonucleotide concentration ...
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bioRxiv - Zoology 2020Quote: ... Each lysate was confirmed to express the appropriate recombinant protein at the expected size using an anti-V5 antibody produced in rabbit (Sigma V8137). Isoform specificity was tested via immunoblotting all cell lysates (empty vector control ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant MBP fusion protein was concentrated with an Amicon Ultra-15 centrifugal filter unit (Ultracel 3K; Merck Millipore, Darmstadt, Germany) and used directly for electrophoretic mobility shift assay (EMSA).
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bioRxiv - Cell Biology 2020Quote: ... Base media for the culture of mESCs were supplemented with 1000 units/mL ESGRO Recombinant mouse LIF protein (EMD Millipore ESG1107), and 2i small molecule inhibitors ...
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bioRxiv - Neuroscience 2021Quote: ... αSyn oligomers were produced by incubating recombinant αSyn to a final concentration of 20 µM with 300 µM DOPAL or dopamine (Sigma-Aldrich) in PBS ...